Translational regulation of DNA repair systems by eIF3a in cancer chemotherapeutic response

作     者：CHEN Juan CUI Jia-jia ZHANG Jian-ting ZHOU Hong-hao LIU Zhao-qian YIN Ji-ye 

作者机构：Department of Clinical PharmacologyXiangya HospitalCentral South University Institute of Clinical Pharmacology Central South University Hunan Key Laboratory of Pharmacogenetics Department of Pharmacology and ToxicologyIndiana University School of Medicine IU Simon Cancer CenterIndiana University School of Medicine 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2016年第30卷第10期

页      面：1050-1051页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：The project supported by National High-tech R&D Program of China 863 Program Grant(2009AA022704) National Natural Science Foundation of China(81573463,81173129,81202595 and NIH Grant CA 94961) 

主　　题：eIF3a DNA repair translation regulation platinum anthracycline cancer chemotherapy 

摘      要：OBJECTIVE To investigate the role of e IF3a in the regulation of DNA repair pathways in cancer chemotherapeutic *** Immunohistochemistry was used to determine the expression of e IF3a in lung and breast cancer tissues followed by association analysis of e IF3a expression with patient′s response to *** overexpression and RNA interference knockdown of e IF3a were carried out in NIH3T3and H1299 cell lines,respectively,to determine the effect of altered e IF3a expression on cellular response to chemotherapeutic drugs by using MTT *** DNA repair capacity of these cells was evaluated by using host-cell reactivation,NHEJ and HR ***-time reverse transcriptase PCR and Western Blot analyses were carried out to determine the effect of e IF3a on the DNA repair genes by using cells with altered e IF3a *** e IF3a expression associates with response of lung and breast cancer patients to platinum and anthracycline.e IF3a knockdown or overexpression,respectively,increased and decreased the cellular resistance to cisplatin and anthracycline anticancer drugs,DNA repair activity,and expression of NER and NHEJ DNA repair *** e IF3a plays an important role in regulating the expression of NER and NHEJ DNA repair proteins which,in turn,contributes to cellular response to DNA-damaging anticancer drugs and patients′response to platinum and anthracycline chemotherapy.