Distinctive quality control method for solid-state fermented Isaria cicadae from strain Ic-17-7 and application in a rat model of type 2 diabetes

作     者：SUN Chang-Sheng WANG Yu-Qin ZHANG Zhong-Liang LIOU Yuligh ZHOU Hong-Hao SUN Chang-Sheng;WANG Yu-Qin;ZHANG Zhong-Liang;LIOU Yuligh;ZHOU Hong-Hao

作者机构：Department of Clinical PharmacologyXiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical PharmacologyCentral South UniversityHunan Key Laboratory of PharmacogeneticsChangsha 410078China Engineering Research Center of Applied Technology of PharmacogenomicsMinistry of EducationChangsha 410078China National Clinical Research Center for Geriatric DisordersChangsha 410008China China Zhejiang Bioasia Life Science InstitutePinghu 314200China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2021年第19卷第12期

页      面：921-929页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by China Spark Program (No.2015GA700011) 

主　　题：Isaria cicadae Quality control Adenosine HEA Type 2 diabetes model Blood glucose and lipid 

摘      要：This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabetes(T2DM). Random amplified polymorphic DNA, sequence-characterized amplified region, and high-performance liquid chromatography(HPLC) were used for the quality control of Ic-17-7fb. The pharmacological effects on streptozocin(STZ)-induced high fat diet(HFD)-fed Albino Wistar rats were evaluated. The rats underwent the following treatments: control, metformin, Ic-17-7fb(0.166 and 0.5 g·kg;) or without treatment. The fasting blood glucose(FBG), blood glucose, total cholesterol(TC), triglyceride(TG), high-density lipoprotein(HDL-c), and low-density lipoprotein(LDL-c) were measured. Ic-17-7fb amplified a single specific band by S11-2-F3 and S11-2-R3 primers. An HPLC-based quality and quantity method was established for industrial application. The contents of adenosine and N;-(2-hydroxyethyl) adenosine(HEA) of the cultivated Ic-17-7fb were analyzed. All of the validation lots of cultured Ic-17-7fb passed the quantity control of the training set(0.90 mg·g;of adenosine and 0.89 mg·g;of HEA). After two weeks of administration, the average FBG was 4.89 ± 0.42(control), 26.10 ± 5.77(model), 23.63 ± 6.15(metformin), 17.96 ± 9.36(Ic-17-7fb for 0.166 g·kg;), and 19.69 ±8.71 mmol·L;(Ic-17-7fb for 0.5 g·kg;). The FBG of Ic-17-7fb(0.166 g·kg;) treatment significantly reduced by 31.19%, compared with the model after two weeks of administration(P 0.01). Metformin, Ic-17-7fb(0.166 g·kg;), and Ic-17-7fb(0.5 g·kg;) reduced TC, TG, HDL-c, and LDL-c compared with the T2DM model treatment at the 6 th week of treatment(P 0.05). This study established the first quality standard for Ic-17-7fb, which can be effectively applied in the treatment of T2DM. The reliable quality control method and pharmacological effect will broaden its application space.