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CCS Chemistry 2023年第5期5卷 1086-1095页

作者： xuelian zheng Pan Peng Cheng Huang Qingquan Lu The Institute for Advanced Studies Wuhan UniversityWuhan 430072

Sustainable and site-selective C–H functionalization is still a challenge because of the comparable properties of bonds of the same ***,an electrochemical C(sp^(2))–H/C(sp^(3))–H coupling with aryl iodides is reported for the first *** C(sp^(2))–H bond outperformed the much weaker C(sp^(2))–I bond participating in C(sp^(2))–H/C(sp^(3))–H *** protocol features mild reaction conditions,good functional group tolerance,and *** products with C–I bonds are versatile moieties for further synthetic manipulation,that can undergo Suzuki,Sonogashira,Ullmann-Ma,Catellani,Heck reactions,and so on to introduce new functional groups into target molecules with good *** investigations showed that the site selectivity of the reaction is enabled by an iodineassisted[3,3]-sigmatropic rearrangement,and the interaction of hypervalent iodine generated in situ with an enolate is the rate-determining step.

关键词： electrosynthesis electrooxidation C–H functionalization oxidative coupling alkylation

Theoretical Studies on the Electronic Structure of Nano-graphenes for Applications in Nonlinear Optics

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Chemical Research in Chinese Universities 2022年第2期38卷 579-587页

作者： CHEN Kaichun zheng xuelian YANG Cuicui TIAN Wei Quan LI Weiqi YANG Ling Chongqing Key Laboratory of Theoretical and Computational Chemistry College of Chemistry and Chemical EngineeringChongqing UniversityHuxi CampusChongqing 401331P.R.China School of Physics Harbin Institute of TechnologyHarbin 150001P.R.China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006P.R.China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage Institute of Theoretical and Simulational ChemistrySchool of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001P.R.China

In this work,azulene is introduced into nano-graphene with coronene center to enhance the second-order nonlinear optical(NLO)*** sum-over-states(SOS)model based calculations demonstrate that dipolar contributions are larger than octupolar contributions to the static first hyperpolarizability(〈β0〉)in most nano-graphenes except those with high symmetry(e.g.,a C2v nano-graphene has octupolar contributionsΦJ=3 up to 59.0%of the〈β0〉).Nano-graphenes containing two parallel orientating azulenes(i.e.,Out-P and Out-Ps)have large dipole moments,while their ground state is *** B/N/BN atoms into the positions with a high spin density transfers the ground state of Out-P and Out-Ps to closed-shell singlet,and the Out-Ps-2N has a large〈β0〉of 1621.67×10−30 *** addition of an electron donor(NH2)at the pentagon end enhances the〈β0〉to 1906.22×10−30 *** two-dimensional second-order NLO spectra predicted by using the SOS model find strong sum frequency generations and difference frequency generations,especially in the near-infrared and visible *** strategies to stabilize the electronic structure and improve the NLO properties of azulene-defect carbon nanomaterials are proposed,and those strategies to engineer nano-graphenes to be semiconducting while maintaining theπ-framework are exten-dable to other similar systems.

关键词： Nonlinear optics Azulene-defect nano-graphene Two-dimensional second-order nonlinear optical spectrum Sum frequency generation Difference frequency generation

Modulation of the Second Order Nonlinear Optical Properties of Helical Graphene Nanoribbons Through Introducing Azulene Defects or/and BN Units

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Chemical Research in Chinese Universities 2022年第4期38卷 974-984页

作者： zheng xuelian LIU Ling YANG Cuicui HE Yuanyuan CHEN Jiu TIAN Wei Quan Chongqing Key Laboratory of Theoretical and Computational Chemistry College of Chemistry and Chemical EngineeringChongqing UniversityChongqing401331P.R.China Department of Materials Science and Engineering Southern University of Science and TechnologyShenzhen518055P.R.China

The current study has obtained excellent potential nonlinear optical(NLO)materials by combining density functional theory methods with sum-over-states model to predict the second order NLO properties of helical graphene nanoribbons(HGNs)through introducing azulene defects or/and BN *** introduction of these functional groups deforms the pristine HGN(compression or tension)and enhances obviously the static first hyperpolarizability(〈β0〉)of system by up to two orders of *** tensor components along the helical axis of HGNs play a dominant role in the total〈β0〉.The azulene defects and the BN units polarize the pristine HGN to different degrees,and the azulenes and contiguous benzenes are involved in the major electron excitations with significant contributions to〈β0〉but the BN units are *** BN-doped chiral HGNs have good kinetic stability and strong second order NLO properties(6.84×10^(5)×10^(−30) esu),and can be a potential candidate of high-performance second order NLO *** predicted two-dimensional second order NLO spectra provide useful information for further exploration of those helicenes for electro-optic applications.

关键词： Helical graphene nanoribbon Azulene defect BN-doping Second order nonlinear optical(NLO)property Sum-over-states model

Boosting genome editing in plants with single transcript unit surrogate reporter systems

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Plant Communications 2024年第6期5卷 32-43页

作者： Xu Tang Qiurong Ren Xiaodan Yan Rui Zhang Li Liu Qinqin Han xuelian zheng Yiping Qi Hongyuan Song Yong Zhang Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science CitySchool of Life SciencesSouthwest UniversityChongqing 400715China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River Ministry of EducationChongqing 400715China College of Horticulture and Landscape Architecture Southwest UniversityChongqing 400715China Department of Biotechnology School of Life Sciences and TechnologyCenter for Informational BiologyUniversity of Electronic Science and Technology of ChinaChengdu 610054China School of Synbiology School of Life ScienceShanxi UniversityTaiyuan 030006China Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA Institute for Bioscience and Biotechnology Research University of MarylandRockvilleMD 20850USA

CRISPR-Cas-based genome editing holds immense promise for advancing plant genomics and crop ***,the challenge of low editing activity complicates the identification of editing *** this study,we introduce multiple single transcript unit surrogate reporter(STU-SR)systems to enhance the selection of genome-edited *** systems use the same single guide RNAs designed for endogenous genes to edit reporter genes,establishing a direct link between reporter gene editing activity and that of endogenous *** strategies are used to restore functional reporter genes after genome editing,including efficient single-strand annealing(SSA)for homologous recombination in STUSR-SSA ***-SR-base editor systems leverage base editing to reinstate the start codon,enriching C-to-T and A-to-G base editing *** results showcase the effectiveness of these STU-SR systems in enhancing genome editing events in the monocot rice,encompassing Cas9 nuclease-based targeted mutagenesis,cytosine base editing,and adenine base *** systems exhibit compatibility with Cas9 variants,such as the PAM-less SpRY,and are shown to boost genome editing in Brassica oleracea,a dicot vegetable *** summary,we have developed highly efficient and versatile STU-SR systems for enrichment of genome-edited plants.

关键词： CRISPR-Cas9 single transcript unit STU surrogate reporter SR single strand annealing SSA cyto-sine base editing adenine base editing

A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants

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Molecular Plant 2016年第7期9卷 1088-1091页

作者： Tang, Xu zheng, xuelian Qi, Yiping Zhang, Dengwei Cheng, Yan Tang, Aiting Voytas, Daniel F. Zhang, Yong Univ Elect Sci & Technol China Sch Life Sci & Technol Dept Biotechnol Chengdu 610054 Peoples R China Univ Minnesota Dept Genet Cell Biol & Dev Minneapolis MN 55455 USA Univ Minnesota Ctr Genome Engn Minneapolis MN 55455 USA East Carolina Univ Dept Biol Greenville NC 27834 USA

The Streptococcus pyogenes CRISPR-Cas9 system effectively mediates RNA-guided DNA double-strand breaks and is used for genome editing in many different organisms, including plants （Puchta, 2016）. CRISPR-Cas9 is a two-component system in which the Cas9 protein is expressed from a Pol II promoter （Lowder et al., 2015）. In contrast, the sgRNAs are typically expressed from Pol III promoters, such as U6 and U3.

关键词： PROTOPLASTS CATALYTIC RNA

An improved plant prime editor for efficient generation of multiple-nucleotide variations and structural variations in rice

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Plant Communications 2024年第9期5卷 21-24页

作者： Zhaohui Zhong Tingting Fan Yao He Shishi Liu xuelian zheng Yang Xu Jingqi Ren Hua Yuan zhengyan Xu Yong Zhang Department of Biotechnology School of Life Sciences and TechnologyCenter for Informational BiologyUniversity of Electronic Science and Technology of ChinaChengdu 610054China Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science CitySchool of Life SciencesSouthwest UniversityChongqing 400715China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Rice Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina

Dear Editor,The ability to manipulate single-nucleotide variations(SNVs),multiple-nucleotide variations(MNVs),and structural variations(SVs)in rice genomes is crucial for studying gene function and improving agricultural ***,these manipulations were achieved mainly through homology-directed repair,which has limited efficiency in plants.

关键词： structural editor nucleotide

IsDge10 is a hypercompact TnpB nuclease that confers efficient genome editing in rice

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Plant Communications 2024年第11期5卷 6-9页

作者： Rui Zhang Xu Tang Yao He Yangcun Li Wei Wang Yawei Wang Danning Wang xuelian zheng Yiping Qi Yong Zhang Department of Biotechnology School of Life Sciences and TechnologyCenter for Informational BiologyUniversity of Electronic Science and Technology of ChinaChengdu 610054China Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Chongqing Key Laboratory of Tree Germplasm Innovation and UtilizationSchool of Life SciencesSouthwest UniversityChongqing 400715China State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 311400 ZhejiangChina Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA Institute for Bioscience and Biotechnology Research University of MarylandRockvilleMD 20850USA

Dear Editor,Cas9 and Cas12a have been widely applied in genome engineering in both plant and human cells(Tang and Zhang,2023).However,the relatively large sizes restrict their delivery into cells via viral *** hypothetical ancestors of Cas9 and Cas12a,IscB and TnpB have been reported as RNA-guided DNA endonucleases suitable for genome editing in human cells(Han et al.,2023;Xiang et al.,2023).More recently,a eukaryotic RNA-guided endonuclease named Fanzor has demonstrated genome editing capabilities in human cells(Saito et al.,2023).These nucleases,such as IsDge10,are significantly smaller(∼390 amino acids for IsDge10)compared to Cas9 and Cas12a(e.g.,∼1300 amino acids for SpCas9).However,the applicability of TnpB,IscB,or Fanzor for plant genome engineering remained ***,we evaluated a series of nucleases from the TnpB,IscB,and Fanzor families and successfully developed a miniature plant genome editor using IsDge10,a TnpB nuclease from Deinococcus geothermalis.

关键词： sizes restrict compact

Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice

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Rice science 2019年第2期26卷 98-108,I0007-I0015页

作者： WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan YU Deshui zheng xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong College of Life Sciences Nankai University Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China

Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding.

关键词： rice NAC transcription factor CRISPR Cas9 RNA-seq salinity

Plant Prime Editors Enable Precise Gene Editing in Rice Cells

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Molecular Plant 2020年第5期13卷 667-670页

作者： Xu Tang Simon Sretenovic Qiurong Ren Xinyu Jia Mengke Li Tingting Fan Desuo Yin Shuyue Xiang Yachong Guo Li Liu xuelian zheng Yiping Qi Yong Zhang Department of Biotechnology School of Life Sciences and TechnologyCenter for Informational BiologyUniversity of Electronic Science and Technology of ChinaRoom 216Main BuildingNo.4Section 2North Jianshe RoadChengdu 610054China Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA Hubei Academy of Agricultural Sciences Wuhan 430064China lnstitute for Bioscience and Biotechnology Research University of MarylandRockvilleMD 20850USA

Genome editing is revolutionizing plant research and crop ***-specific nucleases(SSNs)such as zinc finger nuclease(ZFN)and TAL effector nuclease(TALEN)have been used to create site-specific DNA double-strand breaks and to achieve precise DNA modifications by promoting homology-directed repair(HDR)(Steinert et al.,2016;Voytas,2013).Later,RNA-guided SSNs such as CRISPR-Cas9,Cas12a,Cas12b,and their variants were applied for genome editing in plants(Li et al.,2013;Nekrasov et alM 2013;Tang et al.,2017;Zhong et al.,2019;Ming et al.,2020;Tang et al.,2019).However,HDR relies on simultaneous delivery of SSNs and DNA donors,which has been challenging in plants(Steinert et al.,2016;Zhang et aL,2019).Another challenge for realizing efficient HDR in plants is that DNA repair favors nonhomologous end joining(NHEJ)pathways over HDR in most cell types(Puchta,2005;Qi et al.,2013).

关键词： precise Prime Plant

Versatile plant genome engineering using anti-CRISPR-Cas12a systems

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Science China Life Sciences 2024年第12期67卷 2730-2745页

作者： Yao He Shishi Liu Long Chen Dongkai Pu Zhaohui Zhong Tang Xu Qiurong Ren Chuan Dong Yawei Wang Danning Wang xuelian zheng Fengbiao Guo Tao Zhang Yiping Qi Yong Zhang Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization School of Life Sciences Southwest University Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China Sichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education College of AgricultureYangzhou University Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University Department of Respiratory and Critical Care Medicine Zhongnan Hospital of Wuhan University Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education and School of Pharmaceutical Sciences Wuhan University Department of Plant Science and Landscape Architecture University of MarylandCollege Park Institute for Bioscience and Biotechnology Research University of Maryland

CRISPR-Cas12a genome engineering systems have been widely used in plant research and crop breeding. To date, the performance and use of anti-CRISPR-Cas12a systems have not been fully established in plants. Here, we conduct in silico analysis to identify putative anti-CRISPR systems for Cas12a. These putative anti-CRISPR proteins, along with known anti-CRISPR proteins, are assessed for their ability to inhibit Cas12a cleavage activity in vivo and in planta. Among all anti-CRISPR proteins tested, AcrVA1 shows robust inhibition of Mb2Cas12a and LbCas12a in E. coli. Further tests show that Acr VA1 inhibits LbCas12a mediated genome editing in rice protoplasts and stable transgenic lines. Impressively, co-expression of Acr VA1 mitigates off-target effects by CRISPR-LbCas12a, as revealed by whole genome sequencing. In addition, transgenic plants expressing AcrVA1 exhibit different levels of inhibition to LbCas12a mediated genome editing, representing a novel way of fine-tuning genome editing efficiency. By controlling temporal and spatial expression of AcrVA1, we show that inducible and tissue specific genome editing can be achieved in plants. Furthermore, we demonstrate that AcrVA1 also inhibits Lb Cas12a-based CRISPR activation(CRISPRa) and based on this principle we build logic gates to turn on and off target genes in plant cells. Together, we have established an efficient anti-CRISPR-Cas12a system in plants and demonstrate its versatile applications in mitigating off-target effects, finetuning genome editing efficiency, achieving spatial-temporal control of genome editing, and generating synthetic logic gates for controlling target gene expression in plant cells.

关键词： anti-CRISPR Cas12a AcrVA1 off-target effects fine-tuning genome editing inducible and tissue specific genome editing CRISPRa synthetic logic circuit