Plant Prime Editors Enable Precise Gene Editing in Rice Cells
作者机构：Department of BiotechnologySchool of Life Sciences and TechnologyCenter for Informational BiologyUniversity of Electronic Science and Technology of ChinaRoom 216Main BuildingNo.4Section 2North Jianshe RoadChengdu 610054China Department of Plant Science and Landscape ArchitectureUniversity of MarylandCollege ParkMD 20742USA Hubei Academy of Agricultural SciencesWuhan 430064China lnstitute for Bioscience and Biotechnology ResearchUniversity of MarylandRockvilleMD 20850USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2020年第13卷第5期
页 面：667-670页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the National Transgenic Major Project of China 国家自然科学基金 the Sichuan Youth Science and Technology Foundation the Science Strength Promotion Program of UESTC to Y.Z supported by the National Science Foundation Plant Genome Research Program grant Biotechnology Risk Assessment Grant Program competitive from the U.S. Department of Agriculture to Y.Q
摘 要：Genome editing is revolutionizing plant research and crop ***-specific nucleases(SSNs)such as zinc finger nuclease(ZFN)and TAL effector nuclease(TALEN)have been used to create site-specific DNA double-strand breaks and to achieve precise DNA modifications by promoting homology-directed repair(HDR)(Steinert et al.,2016;Voytas,2013).Later,RNA-guided SSNs such as CRISPR-Cas9,Cas12a,Cas12b,and their variants were applied for genome editing in plants(Li et al.,2013;Nekrasov et alM 2013;Tang et al.,2017;Zhong et al.,2019;Ming et al.,2020;Tang et al.,2019).However,HDR relies on simultaneous delivery of SSNs and DNA donors,which has been challenging in plants(Steinert et al.,2016;Zhang et aL,2019).Another challenge for realizing efficient HDR in plants is that DNA repair favors nonhomologous end joining(NHEJ)pathways over HDR in most cell types(Puchta,2005;Qi et al.,2013).
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