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Journal of Integrative Agriculture 2013年第2期12卷 319-326页

作者： LIU Da-cheng ZHOU Xiang-li zhao pei-ting GAO Min HAN Hao-qi HU Hong-lian College of Veterinary Medicine Inner Mongolia Agricultural University Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease of Ministry of Agriculture Animal Nutrition Institute of Agriculture and Animal Husbandry Academy of Inner Mongolia

Six rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute rtmainal acidosis （SARA） inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate （NFC） to neutral detergent fiber （NDF） ratio from 1.02 （stage 1） to 1.24 （stage 2）, 1.63 （stage 3） and 2.58 （stage 4）. A dynamic pH monitoring system, real-time fluorescent quantitative PCR and conventional anaerobic culture were used to assess changes in ruminal pH and microbiota. Results indicated that rumen fermentation patterns changed significantly with increased NFC：NDF ratio. The decline in ruminal pH was caused by increased ruminal total volatile fatty acids （TVFA）, which was mainly attributed to a significant increase in ruminal butyrate, rather than the accumulation of ruminal lactic acid. In addition, in the course of SARA, the number of rumen microoganisms altered significantly, with increases in ruminal amylolytic bacteria, Lactobacilli, Streptococcus bovis and Megasphaera elsdenii, the latter particularly dramatically indicating that it may be the main factor responsible for the increase in butyrate, and decrease in protozoa.

关键词： rumen fermentation rumen microorganisms subacute ruminal acidosis goat

交直流混合外送系统风火容量配比对暂态稳定性的影响

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内蒙古工业大学学报（自然科学版） 2021年第3期40卷 226-232页

作者：赵培廷徐涛内蒙古工业大学电力学院呼和浩特010050

风火打捆经交直流外送的混合输电方式,充分利用了交流和直流输电系统的各自优势,不仅提高了外送容量而且增强了远距离输电的灵活性和控制力.但大规模风电并入交直流混合外送系统后,风电的波动性和特有的控制方式对系统暂态稳定性造成了... 详细信息

风火打捆经交直流外送的混合输电方式,充分利用了交流和直流输电系统的各自优势,不仅提高了外送容量而且增强了远距离输电的灵活性和控制力.但大规模风电并入交直流混合外送系统后,风电的波动性和特有的控制方式对系统暂态稳定性造成了一定的不利影响.为确保风电并入后混合输电系统的暂态稳定性,深入研究风火容量配比对系统的暂态稳定性的影响意义重大.利用相关文献所建立的风火打捆经交直流外送的混合系统模型及理论推导,定性总结了风电接入对系统暂态稳定性的影响规律,在PSAT仿真软件中搭建了系统仿真模型并进行了仿真实验.仿真结果验证了所总结规律的合理性,为进一步探索利用风火容量配比改善系统阻尼比,弱化风电并入后对混合系统暂态稳定性的不利影响奠定了基础.

关键词：风火打捆交直流混合系统容量配比暂态稳定性仿真

不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响

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畜牧兽医学报 2015年第1期46卷 96-103页

作者：郭鹏刘大程赵培厅高民胡红莲内蒙古农业大学兽医学院呼和浩特010018 农业部动物疾病临床诊疗技术重点实验室呼和浩特010018 内蒙古农牧业科学院动物营养研究所呼和浩特010030

旨在观察不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,试验分四期进行,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、... 详细信息

旨在观察不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,试验分四期进行,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮,以逐渐增加日粮精料的方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA)。分别利用pH动态监测记录系统和滚管培养法测定瘤胃pH、瘤胃细菌总数、淀粉分解菌及坏死梭形杆菌的浓度;以鲎试验试剂法和荧光分光光度法测定内毒素和组胺含量。结果表明:随着日粮NFC/NDF比的增大,瘤胃pH显著降低(P<0.05),并且瘤胃pH下降速率和下降幅度也随之加快;日粮NFC/NDF比为2.58时,瘤胃内组胺和内毒素的浓度明显增加(P<0.05),坏死梭形杆菌和淀粉分解菌的数量也显著升高(P<0.01)。内毒素、组胺和坏死梭形杆菌三者浓度的异常增加对SARA发生和发展起重要作用。

关键词：亚急性瘤胃酸中毒瘤胃pH 瘤胃细菌内毒素组胺

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乡村学校体育的现实困囿与发展路径

体育文化导刊 2024年第6期 1-5页

作者：赵姵婷郭立亚陈凯黄丽西南大学体育学院重庆400715

运用文献资料等方法,分析乡村学校体育的价值、现实困囿,并提出发展路径。价值:发挥主体作用,激活乡村振兴内生动力;推动教育发展,促进构建高质量教育体系;培育后备人才,助力建成现代化体育强国。现实困囿:体育场馆分布不均,体育师资结... 详细信息

运用文献资料等方法,分析乡村学校体育的价值、现实困囿,并提出发展路径。价值:发挥主体作用,激活乡村振兴内生动力;推动教育发展,促进构建高质量教育体系;培育后备人才,助力建成现代化体育强国。现实困囿:体育场馆分布不均,体育师资结构性短缺,体育育人功能发挥不佳,学校体育开展力度不足。发展路径:优化资源分配,满足不同地区学校发展需求;坚持强基固本,合力建设高质量师资队伍;聚焦育人内涵,充分利用各类资源创新育人模式;更新教学理念,不断丰富学校课外活动内容。

关键词：乡村体育乡村学校体育学校体育

Protein nanoparticles as potent delivery vehicles for polycytosine RNA-binding protein one

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World Journal of Diabetes 2025年第1期16卷 222-225页

作者： Zi-Yu zhao pei-Li Luo Xia Guo Zheng-Wei Huang State Key Laboratory of Bioactive Molecules and Druggability Assessment Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative DrugsCollege of PharmacyJinan UniversityGuangzhou 511436Guangdong ProvinceChina

Ma et al recently reported in the World Journal of Diabetes that ferroptosis occurs in osteoblasts under high glucose conditions,reflecting diabetes *** condition could be protected by the upregulation of the gene encoding polycytosine RNA-binding protein 1(PCBP1).Additionally,Ma et al used a lentivirus infection system to express *** the authors’method of administration can be improved in terms of stability and cost,we propose delivering PCBP1 to treat type 2 diabetic osteoporosis by encapsulating it in protein ***,PCBP1 is small and ***,intravenous injection can help deliver PCBP1 across the mucosa while avoiding acid and enzyme-catalyzed ***,incorporating PCBP1 into nanoparticles prevents its interaction with water or oxygen and protects PCBP1’s structure and ***,the safety of the protein materials and the industrialization techniques for large-scale production of protein nanoparticles must be comprehensively investigated before clinical application.

关键词： Polycytosine RNA-binding protein 1 Protein nanoparticle Osteoblast Ferroptosis Diabetes

Differential distribution of PINK1 and Parkin in the primate brain implies distinct roles

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Neural Regeneration Research 2025年第4期20卷 1124-1134页

作者： Yanting Liu Wei Huang Jiayi Wen Xin Xiong ting Xu Qi Wang Xiusheng Chen Xianxian zhao Shihua Li Xiaojiang Li Weili Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan UniversityJinan UniversityGuangzhouGuangdong ProvinceChina

The vast majority of in vitro studies have demonstrated that PINK1 phosphorylates Parkin to work together in mitophagy to protect against neuronal ***,it remains largely unclear how PINK1 and Parkin are expressed in mammalian *** has been difficult to address because of the intrinsically low levels of PINK1 and undetectable levels of phosphorylated Parkin in small *** this issue is critical for elucidating the in vivo roles of PINK1 and ***,we showed that the PINK1 kinase is selectively expressed as a truncated form(PINK1–55)in the primate *** the present study,we used multiple antibodies,including our recently developed monoclonal anti-PINK1,to validate the selective expression of PINK1 in the primate *** found that PINK1 was stably expressed in the monkey brain at postnatal and adulthood stages,which is consistent with the findings that depleting PINK1 can cause neuronal loss in developing and adult monkey ***1 was enriched in the membrane-bound fractionations,whereas Parkin was soluble with a distinguishable *** double staining experiments showed that PINK1 and Parkin did not colocalize under physiological conditions in cultured monkey astrocytes,though they did colocalize on mitochondria when the cells were exposed to mitochondrial *** findings suggest that PINK1 and Parkin may have distinct roles beyond their well-known function in mitophagy during mitochondrial damage.

关键词： neurodegeneration Parkin Parkinson’s disease PINK1 subcellular distribution

The cGAS-Sting-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou ting Li Wei Zhang Jian Wu Hui Hong Wei Quan Xinyu Qiao Chun Cui Chenmeng Qiao Weijiang zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 Sting type I interferon

RGS4 promotes the progression of gastric cancer through the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition

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World Journal of Gastroenterology 2025年第2期31卷 113-127页

作者： Peng-Yu Chen pei-Yao Wang Bang Liu Yang-Pu Jia zhao-Xiong Zhang Xin Liu Dao-Han Wang Yong-Jia Yan Wei-Hua Fu Feng Zhu Department of General Surgery Tianjin Medical University General HospitalTianjin Medical UniversityTianjin 300052China Department of General Surgery Jincheng People’s HospitalJincheng 048000Shanxi ProvinceChina

BACKGROUND Regulator of G protein signaling(RGS)proteins participate in tumor formation and metastasis by acting on theα-subunit of heterotrimeric G *** speci-fic effect of RGS,particularly RGS4,on the progression of gastric cancer(GC)is not yet *** To explore the role and underlying mechanisms of action of RGS4 in GC *** The prognostic significance of RGS4 in GC was analyzed using bioinformatics based public databases and verified by immunohistochemistry and quantitative polymerase chain reaction in 90 patients with *** assays were employed to assess the carcinogenic impact of RGS4,and the mechanism of its possible influence was detected by western blot analysis.A nude mouse xenograft model was established to study the effects of RGS4 on GC growth in *** RGS4 was highly expressed in GC tissues compared with matched adjacent normal *** RGS4 expression was correlated with increased tumor-node-metastasis stage,increased tumor grade as well as poorer overall survival in patients with *** experiments demonstrated that RGS4 knockdown suppressed GC cell proliferation,migration and ***,xenograft experiments confirmed that RGS4 silencing significantly inhibited tumor ***,RGS4 knockdown resulted in reduced phosphorylation levels of focal adhesion kinase,phosphatidyl-inositol-3-kinase,and protein kinase B,decreased vimentin and N-cadherin,and elevated *** High RGS4 expression in GC indicates a worse prognosis and RGS4 is a prognostic ***4 influences tumor progression via the focal adhesion kinase/phosphatidyl-inositol-3-kinase/protein kinase B pathway and epithelial-mesenchymal transition.

关键词： Gastric cancer Prognosis Regulator of G protein signaling 4 Focal adhesion kinase Epithelial-mesenchymal transition

Intestinal glucagon-like peptide-1:A new regulator of impaired counterregulatory responses to hypoglycemia in type 1 diabetes mellitus

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World Journal of Diabetes 2025年第1期16卷 217-221页

作者： Le-Rong Liu Yuan-Yuan Luo pei-Zhu Su Cong Zhang zhao-Tao Li Department of Endocrinology and Metabolism Nanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Endocrinology Chongqing University Three Gorges HospitalChongqing 404100China Chongqing Municipality Clinical Research Center for Endocrinology and Metabolic Diseases Chongqing University Three Gorges HospitalChongqing 404100China Department of Endocrinology The Second Affiliated HospitalChongqing Medical UniversityChongqing 400013China Department of Gastroenterology The First People's Hospital of FoshanFoshan 528000Guangdong ProvinceChina

In this article,we review the study by Jin et al,which examined the role of intestinal glucagon-like peptide-1(GLP-1)in counterregulatory responses to hypoglycemia in patients with type 1 diabetes mellitus(T1DM).With the global rise of T1DM,there is an increased burden on society and healthcare *** to insulin therapy and islet dysfunction,T1DM patients are highly vulnerable to severe hypoglycemia,a leading cause of *** healthy individuals,counterregulatory mechanisms restore blood glucose during hypoglycemia,but repeated episodes impair these *** et al demonstrated that overexpression of GLP-1 attenuates the sympathetic-adrenal reflex and disrupts the secretion of counterregulatory hormones such as glucagon during hypoglycemia,leading to counterregulatory *** findings highlight the critical role of GLP-1 in the impaired counterregulatory response to hypoglycemia in T1DM patients and provide new insights into the potential application of GLP-1-related therapies in T1DM patients.

关键词： Glucagon-like peptide-1 Impaired counterregulation Type 1 diabetes mellitus Sympathetic-adrenal reflex Glucagon

Deep Eutectic Solvent-assisted Synthesis of Nitrogen-doped Carbon Quantum Dots for Cell Imaging

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Chemical Research in Chinese Universities 2020年第5期36卷 955-961页

作者： LI Long HUANG Yan zhao pei MIAO Haochi zhao ting School of Chemistry and Chemical Engineenng Jiangsu UniversityZhenjiang 212013P.R.China

Carbon quantum dots(CQDs)are widely used in fluorescence imaging due to their negligible toxicity,low-cost and excellent Huorescence *** fluorescence properties of CQDs are greatly a fleeted by the synthesis method,passivation agent and reaction *** this study,the nitrogen-doped CQDs(N-CQDs)were synthesized by a facile microwave-assisted method with citric acid(CA)as a carbon source and deep eutectic solvents as a N-dopaiit as well as the reaction *** detailed characterizations,the as-prepared N-CQDs exliibited near spherical morphology with an average size of(2.64±0.55)***,the abundant hydrophilic limctional groups including hydroxyl,carboxyl,amine and quaternary-ammonium were present on the surface of the ***-CQDs displayed the excitation-dependent fluorescence feature under the excitation wavelength of 350—80 ***,the N-CQDs could be efficiently uptaken by RAW264.7 cells and emitted bright green ***,the resultant N-CQDs showed the low cytotoxicity in living cells,suggesting the great potential in bio-imaging applications.

关键词： N-Dopant carbon quantum dot Microwave Deep eutectic solvent Green light emission Cell imaging