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Cloning of rat sp56, the homologue of mouse sperm ZP3.receptor-sp56

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cell Research 2003年第2期13卷 121-129页

作者： SS KOIDE The Population Council Center for Biomedical Research 123. York Avenue New York New York 100021 USA

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3.(mZP3. receptor. Up to date, its homologue has only been cloned from guinea pig. namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743.bp and 93. bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743.bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743.bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a -2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr -42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivi

关键词： ZP3.receptor sp56 rat testis sperm cloning.

CMV-hFasL transgenic mice are sensitive to low doses of streptozotocin-induced type Ⅰ diabetes mellitus

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Acta Pharmacologica Sinica 2003年第12期 25-30页

作者： LIN Bo, zhang Zhen-Lin~2, YU Lu-Yang, GUO Li-He~3.institute of biochemistry and {3. biology, shanghai lnstitute for biological Sciences, chinese academy of Sciences shanghai 20003. ~2Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, shanghai Jiaotong University Affiliated Sixth People's Hospital, shanghai 20023., {13.

AIM: To investigate the role of Fas-FasL pathway in the pathogenesis of streptozotocin (STZ)-induced type Ⅰ diabetes mellitus. METHODS: Low dose injections of STZ were used to induce type Ⅰ diabetes mellitus in the CMV-hFasL transgenic mice. Blood glucose concentration was measured with Glucotrand Plus blood glucose test strips. Expression of hFasL was detected by RT-PCR and Western blotting. The severity of insulitis was determined by histological examination. Expressions of IL-1β and TNF-α mRNA in the pancreas were detected by semi-quantitative RT-PCR analysis. Fas expression in apoptotic RIN-5F cells was also confirmed by RT-PCR in vitro. RESULTS: hFasL was expressed in the islets of CMV-hFasL transgenic mice. The transgenic mice were sensitive to diabetic induction than the control WT mice. IL-1β and TNF-α expressions in the pancreas of CMV-hFasL transgenic mice were far more than that in WT mice. We also found STZ and IL-1β could both induce higher expression of Fas in RIN-5F. The combining

关键词： transgenic mice Fas Fas ligand type Ⅰ diabetes mellitus streptozotocin apoptosis interleukin-1 tumor necrosis factor

An armed oncolytic adenovirus system, ZD55-gene, demonstrating potent antitumoral efficacy

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cell Research 2003年第6期13卷 481-489页

作者： ZI LAI zhang, WEI Guo ZOU, CHUN XIA LUO, BING HUA LI, JIN HUI WANG, LAN YING SUN, QI JUN QIAN, XIN YUAN LIULaboratory of Biotechnology, institute of biochemistry and {3. biology, shanghai institutes for biological Sciences, chinese academy of Sciences, shanghai 20003., china. E-mail: xyliu@*** Eastern Heptobiliary Hospital, Second Military Medical University, shanghai 20043., china 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@*** 2Eastern Heptobiliary Hospital Second Military Medical University Shanghai 200438 China 1Laboratory of Biotechnology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. E-mail: xyliu@***

ONYX-015 is an attractive therapeutic adenovirus for cancer because it can selectively replicate in tumor cells and kill them. To date, clinical trials of this adenovirus have demonstrated marked safety but not potent enough when it was used alone. In this paper, we put forward a novel concept of Gene-ViroTherapy strategy and in this way, we constructed an armed therapeutic oncolytic adenovirus system, ZD55-gene, which is not only deleted of ElB 55-kD gene similar to ONYX-015, but also armed with foreign antitumor gene. ZD55-gene exhibited similar cytopathic effects and replication kinetics to that of ONYX-015 in vitro. Importantly, the carried gene is expressed and the expression level can increase with the replication of virus. Consequently, a significant antitumoral efficacy was observed when ZD55-CD/5-FU was used as an example in nude mice with subcutaneous human SW620 colon cancer. Our data demonstrated that ZD55-gene, which utilizing the Gene-ViroTherapy strategy, is more efficacious than each ind

关键词： ZD55-gene oncolytic adenovirus ElB 55-kD gene cancer Gene-ViroTherapy.

Hyaluronan Promotes Tumor Lymphangiogenesis and Intralymphantic Tumor Growth in Xenografts

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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 601-606页

作者： Li-Xia GUO Ke ZOU Ji-Hang JW Hong XIE Laboratory of Biotherapy,institute of biochemistry and cell {3.,shanghai institutes for biological Sciences,chinese academy of Sciences,Graduate School of the chinese academy of Sciences,shanghai 20003.,{13. LaboratoryofBiotherapy InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChinese

Hyaluronan (HA),a high molecular weight glycosaminoglycan in the extracellular matrix,hasbeen implicated in the promotion of malignant phenotypes,including tumor ***,little isknown about the effect of HA on tumor-associated *** this study,mouse hepatocellularcarcinoma Hca-F cells combined with or without HA were injected subcutaneously into C3./Hej mice,thenangiogenesis and lymphangiogenesis of implanted tumors were examined by immunostaining for platelet-endothelial cell adhesion molecule-1 and lymphatic vascular endothelial hyaluronan receptor-1 ***,we found HA promotes tumor lymphangiogenesis and the occurrence of intratumoral lymphaticvessels,but has little effect on tumor ***,HA also promotes intralymphatic tumorgrowth,although it is not sufficient to potentiate lymphatic *** results suggest that HA,which is elevated in most malignant tumor stroma,may also play a role in tumor progression by promotinglymphangiogenesis.

关键词： hyaluronan tumor lymphangiogenesis intralymphatic tumor growth

EMT or apoptosis: a decision for TGF-β

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cell Research 2007年第4期17卷 289-290页

作者： Jianguo Song ~1Laboratory of Molecular cell biology,institute of {3. and cell biology,shanghai institutes for biological Sciences,chinese academy of Sciences,shanghai 20003.,{13. aboratoryofMolecularcellbiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China

Apoptosis and epithelial-to-mesenchymal transition(EMT)are very fundamental physiological *** are both independent and interrelated events in nor-mal development and in maintaining body *** recent years,EMT has emerged as a focus of studiesin cancer research,and increasing data indicate that EMTfunctions as a central step in the invasion and metastasisof some tumor *** the cytoskeleton rearrange-ment and subsequent morphological changes,EMT is oftencharacterized by the dissolution or attenuation of epithelialadhesion and other polarized structures,for example,tightjunctions,and by accordingly the acquisition of migratoryand invasive properties of the cells undergoing *** these changes,EMT can be associated with transcrip-tional or expressional alterations of some epithelial andmesenchymal *** of apoptosis and EMT are linked withvarious pathological processes,such as fibrosis of liver

关键词： a decision for TGF EMT or apoptosis

Leukemia,an effective model for chemical biology and target therapy

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Acta Pharmacologica Sinica 2007年第9期28卷 1316-1324页

作者： Guo-qiang CHEN~(2,3.4),Li-shun WANG~2,Ying-li WU~2,Yun YU~2~2 Department of Pathophysiology,Key Laboratory of cell Differentiation and Apoptosis of chinese Ministry of Education,shanghai Jiao TongUniversity School of Medicine(SJTU-SM,formerly shanghai Second Medical University),shanghai 200025,china,~3.institute of HealthScience,SJTU-SM/shanghai institutes for biological Sciences,shanghai 200025,china Department of Pathophysiology Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education Shanghai Jiao Tong University School of Medicine (SJTU-SM formerly Shanghai Second Medical University) Shanghai China institute of Health Science SJTU-SM/Shanghai Institutes for Biological Sciences Shanghai China

The rapid rise of chemical biology aimed at studying signaling networks for basiccellular activities using specific,active small molecules as probes has greatlyaccelerated research on pathological mechanisms and target therapy of *** research is especially important for malignant tumors such as leukemia,aheterogeneous group of hematopoietic malignancies that occurs *** use of a chemical approach combined with genetic manipulation,great progresshas been achieved over the past few decades on the biological,molecular andcytogenetic aspects of leukemia,and in its diagnosis and *** particular,discoveries of the clinical effectiveness of all-trans retinoic acid and arsenictrioxide in the treatment of acute promyelocytic leukemia and the kinase inhibitorsImatinib and Dasatinib in the treatment of chronic myelogenous leukemia not onlymake target therapy of leukemia a reality,but also push mechanisms of leukemo-genesis and leukemic cell activities *** review will outline advances inchemical biology that help our understanding of the molecular mechanisms of celldifferentiation and apoptosis induction and target therapy of leukemia.

关键词： leukemia chemical biology target therapy retinoic acid arsenic trioxide differentia tion apoptosis

Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes with Virus Infection

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 22-28页

作者： Ming-Min ZHAO~(1,2),De-Rong AN~(1*),Jian ZHAO~2,Guang-Hua HUANG~3.Zu-Hua HE~4,and Jiang-Ye CHEN~3. ~1 College of Plant Protection,Northwest Science and Technology University of Agriculture and Forestry,Yangling 712100,china ~2 College of Agriculture,Yangtze University,Jingzhou 43.025,china ~3.State Key Laboratory of Molecular biology,{3. of biochemistry and cell biology,shanghai institutes for biological Sciences, chinese academy of Sciences,shanghai {13.,china ~4 State Key Laboratory of Plant Molecular Genetics,{3. of Plant Physiology and Ecology,shanghai institutes for biological Sciences, chinese academy of Sciences,shanghai {13.,china College of Plant Protection Northwest Science and Technology University of Agriculture and Forestry Yangling 712100 China

RNA-interference (RNAi) silences gene expression by'guiding mRNA degradation in asequence-specific *** interfering RNA (siRNA),an intermediate of the RNAi pathway,has beenshown to be very effective in inhibiting virus infection in mammalian cells and cultured plant ***,wereport that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) couldinhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-asso-ciated 126 kDa protein in intact plant *** results indicate that transiently expressed shRNA efficientlyinterfered with TMV *** interference observed is sequence-specific,and time-and *** expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumbermosaic virus (CMV),an unrelated *** order to interfere with TMV accumulation in tobaccoleaves,it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV *** support the view that RNAi opens the door for novel therapeutic procedures against virus *** propose that a combination of the RNAi technique and Agrobacterium-mediated transient expressioncould be employed as a potent antiviral treatment in plants.

关键词： tobacco mosaic virus 126 kDa protein RNA interference short hairpin RNA

Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

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Acta Pharmacologica Sinica 2006年第1期27卷 80-90页

作者： Zhao HE~(2,3. Jing-jing LI~(2,3. Chang-hong ZHEN~(3.4) Lin-ying FENG~3.Xiao-yan DING~(2,5)2 Laboratory of Molecular and cell biology and Laboratory of Stem cell biology,institute of biochemistry and cell biology:shanghai institutes for biological Sciences,chinese academy of Sciences,shanghai 20003.,china 3.shanghai institute of Materia Medica.chinese academy of Sciences.shanghai 201203.china 4 Graduate School of the chinese academy of Sciences,shanghai 20003.,china. LaboratoryofMolecularandcellbiologyandLaboratoryofStemcellbiology InstituteofBiochemistryandCellBiology shanghaiinstitutesforbiologicalSciences ChineseAcademyofSciencesShanghai200031China

Aim:Leukemia inhibitory factor (LIF),a pleiotropic cytokine,has been used ex-tensively in the maintenance of mouse embryonic stem cell *** thiscurrent work,we examined the effect of the LIF signaling pathway in embryonicstem (ES) cell differentiation to a neural ***:In the presence of LIF(1000U/mL),the production of neuronal cells derived from embryoid bodies (EB)was tested under various culture *** of the LIF pathway wasexamined with specific *** effects of cell apoptosis and proliferationon neural differentiation were *** cell differentiation into three-germlayers was ***:Under various culture conditions,neuronal differ-entiation was increased in the presence of *** the LIF-activated STAT3.ignaling pathway with specific inhibitors abolished the neuronal differentiationof ES cells,whereas inhibition of the LIF-activated MEK signaling pathway im-paired the differentiation of ES cells toward a glial *** suppressed cellapoptosis and promoted cell proliferation during ES cell *** inhib-ited the differentiation of ES cells to both mesoderm and extraembryonic endo-derm fates,but enhanced the determination of neural ***:These results suggest that LIF plays a positive role during the differentiation ofES cells into neuronal cells.

关键词： leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies

A20 overexpression under control of mouse osteocalcin promoter in MC3.3.E1 cells inhibited tumor necrosis factor-alpha-induced apoptosis

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Acta Pharmacologica Sinica 2006年第9期27卷 1231-1237页

作者： Yue-juan QIN~2 Zhen-lin zhang~(2,4)Lu-yang YU~3.Jin-wei HE~2 Ya-nan HOU~3.Tian-jin LIU~3.Jia-cai WU~3.Song-hua WU~2 Li-he GUO~3.2 Center for Preventing and Treating Osteoporosis,Osteoporosis Research Unit,shanghai Sixth People's Hospital,shanghai Jiao Tong University,shanghai 20023.,china 3.institute of biochemistry and cell biology,shanghai institutes for biological Science,chinese {13. of Sciences,shanghai 20003.,china CenterforPreventingandTreatingOsteoporosis OsteoporosisResearchUnitShanghaiSixthPeople'sHospitalShanghaiJiaoTongUniversityShanghai200233China instituteofbiochemistryandcell{3. ShanghaiInstitutesforBiological

Aim:To construct an A20 expression vector under the control of mouse osteocalcinpromoter(OC-A20),and investigate osteoblastic MC3.3.EI cell line,whichstably overexpresses A20 protein prevented tumor necrosis factor(TNF)-alpha-induced ***:OC-A20 vector was constructed by fusing a frag-ment of the mouse osteocalcin gene-2 promoter with human A20 *** the mouse MC3.3.E1 cell line,stably transfected by A20,*** expression of A20 mRNA and A20 protein in the cells weredetected by reverse transcription-polymerase chain reaction(RT-PCR)and West-ern blot analysis,*** determine the specificity of A20 expression inosteoblast,the mouse osteoblastic MC3.3.E1 cell line and mouse embryo fibro-blast NIH3.3.cell line were transiently transfected with *** anti-apoptoticrole of A20 in MC3.3.E1 cells was determined by Flow cytometric analysis(FACS),terminal dUTP nick endo-labeling(TUNEL)and DNA gel electrophoresis analysis(DNA Ladder),***:Weak A20 expression was found in MC3.3.E1 cells with the primers of mouse A20.A20 mRNA and A20 protein expressionwere identified in MC3.3.E1 cells transfected with OC-A20 using RT-PCR andWestern blot *** A20 mRNA expression was found in MC3.3.E1 cellafter MC3.3.E1 cells and NII-/3.3.cells were transient transfected with *** obviously occurred in the rate of apoptosis in the OC-A20 group com-pared with the empty vector(pcDNA3.group by FACS(P3.3.E1 cells andconfirmed that A20 protects osteoblast against TNF-alpha-induced apoptosis.

关键词： A20 osteoblast apoptosis tumor necrosis factor-alpha