Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

作     者：SS KOIDE XIAO BING HE, YUAN CHANG YAN, YI PING LI, SS KOIDE1 Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China2 The Population Council Center for Biomedical Research, 1230 York Avenue, New York, New York 100021, USA

作者机构：The Population Council Center for Biomedical Research 1230 York Avenue New York New York 100021 USA 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第2期

页      面：121-129页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：国家重点基础研究发展计划(973计划)(G199905592) 

主　　题：ZP3 receptor sp56 rat testis sperm cloning. 

摘      要：Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig. namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a -2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr -42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivi