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Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton

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Science China Life Sciences 2025年

作者： Yanlong Li Yinuo fu Yaoyao Li Rui zhang Jing Yang Huanhuan Ma Ling Min Xianlong zhang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

High temperature (HT) stress causes male sterility,leading to reduced upland cotton ***,we identified a key gene,Casein Kinase I (GhCKI),that negatively regulates male fertility in upland cotton under ***,conventional genetic manipulations of GhCKI would result in male sterility,hindering its utilization in breeding ***,we engineered quantitative variation for anther thermotolerance-related traits in upland cotton by creating weak promoter alleles of GhCKI genes,using CRISPR/Cas9 and CRISPR/Cpf1genome ***,we screened and identified two new upland cotton plant lines exhibiting a HT-tolerant phenotype with edited GhCKI promoters,and characterized their corresponding heat-tolerant allelic *** research revealed that the primary reason for the HT tolerance of the GhCKI promoter editing mutants is that the trans-acting factors GhMYB73 and GhMYB4,which positively regulate GhCKI expression under HT,failed to bind and activate the expression of ***,our study not only provides a rapid strategy to generate new beneficial alleles but also offers novel germplasm resources and molecular insights for crop HT tolerance breeding.

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中国常用水稻保持系及恢复系开花灌浆期耐热性评价

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中国水稻科学 2011年第5期25卷 495-500页

作者：符冠富宋健廖西元章秀福熊杰王熹乐明凯陶龙兴中国水稻研究所水稻生物学国家重点实验室浙江杭州310006

以我国常用的15个水稻保持系和26个丰产性较好的恢复系为材料,于主茎见穗当天起连续15d进行高温胁迫处理(9:00-15:00,39～43℃),研究花期高温胁迫对水稻保持系和恢复系结实特性的影响。结果表明,根据高温胁迫指数,可把水稻品种分为热钝... 详细信息

以我国常用的15个水稻保持系和26个丰产性较好的恢复系为材料,于主茎见穗当天起连续15d进行高温胁迫处理(9:00-15:00,39～43℃),研究花期高温胁迫对水稻保持系和恢复系结实特性的影响。结果表明,根据高温胁迫指数,可把水稻品种分为热钝感型、耐热型、不耐热型和热敏感型等4个类型。其中,水稻保持系K22B、博白B和V20B为热钝感型材料,Ⅱ-32B、中浙B和中9B为热敏感型材料;水稻恢复系明恢63耐热性最强,其次为R207和P929,P62-2-2、R8006和P51等耐热性最弱。进一步分析表明,高温胁迫指数与水稻在自然条件下结实率、空壳率和秕谷率之间相关不显著,但与胁迫环境下结实率和空壳率的相关性均达极显著水平。花期高温胁迫致使水稻保持系和恢复系结实率下降,空壳率大幅度上升。

关键词：水稻保持系恢复系高温胁迫育性分析

Staged dendrite suppression for high safe and stable lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 674-683页

作者： Jun Jiang Zhenjie Lu Yanwen Ding Shujun Liu Zhijie Qi Tian Tang Yunfan zhang Zhiyuan Ma Jingwen Sun Liang Xue Wenyao zhang Pan Xiong Xin Wang Junwu Zhu Yongsheng fu Key Laboratory for Soft Chemistry and functional Materials of Ministry of Education Nanjing University of Science and Technology Jiangsu Pylon Battery Co.Ltd

The unavoidable dendrite growth and shuttle effect have long been stranglehold challenges limiting the safety and practicality of lithium-sulfur ***,we propose a dual-action strategy to address the lithium dendrite issue in stages by constructing a multifunctional surface-negatively-charged nanodiamond layer with high ductility and robust puncture resistance on polypropylene （PP） *** uniformly loaded compact negative layer can not only significantly enhance electron transmission efficiency and promote uniform lithium deposition,but also reduce the formation of dendrite during early deposition *** importantly,under the strong puncture stress encountered during the deterioration of lithium dendrite growth under limiting current,the high ductility and robust puncture resistance（145.88 MPa） of as-obtained nanodiamond layer can effectively prevent short circuits caused by unavoidable lithium *** Li||Li symmetrical cells assembled with nanodiamond layer modified PP demonstrated a stable cycle of over 1000 h at 2 mA cm-2with a polarization voltage of only 29.3 ***,the negative charged layer serves as a physical barrier blocking lithium polysulfide ions,effectively mitigating capacity *** improved cells achieved a capacity decay of only 0.042%per cycle after 700 cycles at 3 C,demonstrating effective suppression of dendrite growth and capacity attenuation,showing promising prospect.

关键词： Ductility

稻田冬季保护性耕作对土壤酶活性以及稻米品质的影响

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植物营养与肥料学报 2009年第3期15卷 618-624页

作者：符冠富王丹英徐春梅彭建韩博陶龙兴章秀福中国水稻研究所水稻生物学国家重点实验室浙江杭州310006

为研究稻田冬季保护性耕作对土壤酶活性以及对水稻品质的影响,本试验设计了5个不同种植模式,即稻田免耕稻草全程覆盖种植马铃薯(T1)、免耕直播黑麦草处理(T2)、免耕直播油菜处理(T3)、免耕直播燕麦草处理(T4)以及空闲(CK),以杂交组合国... 详细信息

为研究稻田冬季保护性耕作对土壤酶活性以及对水稻品质的影响,本试验设计了5个不同种植模式,即稻田免耕稻草全程覆盖种植马铃薯(T1)、免耕直播黑麦草处理(T2)、免耕直播油菜处理(T3)、免耕直播燕麦草处理(T4)以及空闲(CK),以杂交组合国稻-6号为材料。研究结果表明:(1)比之对照(CK),T1、T2和T3处理均可显著提高其土壤蛋白酶、中性磷酸酶、过氧化氢酶以及转化酶活性,尤其是T1处理,其土壤酶活性明显高于其他处理;(2)T1、T2和T3处理均能改善稻米品质,和对照(CK)相比,整精米率和蛋白质含量明显提高,垩白度、直链淀粉含量以及胶稠度明显降低;(3)在本试验条件下土壤酶活性对稻米品质影响的程度依次为中性磷酸酶>蛋白酶>过氧化氧酶>转化酶。

关键词：水稻稻田冬季保护性耕作模式土壤酶活性稻米品质

Calibration of the Solar X-ray Detector on-board the Macao Science Satellite-1B for Soft X-ray Detection

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Science China Earth Sciences 2025年第1期68卷 11-26页

作者： Man-Hei NG Xiaoping zhang Jianwu CHEN Yongqiang SHI fuchang ZUO Wenxi PENG Weichun fu Liansheng LI Pengwei LUO Chi-Long TANG Guozheng zhang Zhiwu MEI Li WANG Cunhui LI Jiaming OU Yanyan XU State Key Laboratory of Lunar and Planetary Sciences Macau University of Science and Technology China National Space Administration (CNSA) Macau Center for Space Exploration and Science Space Optoelectronic Measurement and Perception Laboratory Beijing Institute of Control Engineering Institute of High Energy Physics Chinese Academy of Sciences Science and Technology on Vacuum Technology and Physics Laboratory Lanzhou Institute of Physics

The Solar X-ray Detector(SXD) on-board the Macao Science Satellite-1B(MSS-1B) was successfully launched via the Chinese Long March-2C rocket on 21 May 2023, and commenced operations in early June of the same year. The MSS-1B/Soft X-ray Detection Units(SXDUs) employ two silicon drift detectors(SDDs), providing a wide range of energy spectra spanning from 0.7 to 24 keV. Notably, the SXDUs deliver a high-resolution capability of 0.14 keV@5.9 keV and operate with a time cadence of 1 second. Here, we perform thorough calibrations of the MSS-1B/SXDUs, employing a combination of ground experiments and simulations. In addition, quantitative analysis comparing the flux measurements obtained by the MSS-1B/SXDUs to the data collected by the Geostationary Operational Environmental Satellite(GOES), provides compelling evidence of their consistency. furthermore, the preliminary spectral analysis results showcase the robustness and expected performance of the MSS-1B/SXDUs, unlocking their potential for facilitating the study of dynamic evolution of solar flares. Moreover, the innovative MSS-1B/Solar X-ray Detector facilitates concurrent observations of solar soft and hard X-rays, thereby making valuable contributions to the advancements in solar research.

关键词： Macao Science Satellite-1B (MSS-1B) Calibration Solar X-Ray Detector Spectrometer Soft X-rays Solar flares Solar abundances

Development of a general circumferentially nonuniform heat transfer fuel rod model for subchannel analysis and application to helical fuel rods

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Science China Technological Sciences 2025年

作者： Junsen fu Junlong LI Xiaowen WANG Qi zhang Yao XIAO Hanyang GU School of Mechanical Engineering Shanghai Jiao Tong University Key Laboratory of Nuclear Power Systems and Equipment of the Ministry of Education Shanghai Jiao Tong University National Key Laboratory of Nuclear Reactor Technology Nuclear Power Institute of China State Key Laboratory of Nuclear Power Safety Technology and Equipment Shanghai Jiao Tong University

The subchannel analysis method is one of the most crucial transient safety analysis methods in the thermal design of nuclear reactors. The nonuniformity of circumferential heat transfer is slight in conventional pressurized water reactor (PWR)cores, but it is significant in advanced reactors with wire-wrapped or helical fuel rods. Predicting circumferentially nonuniform heat transfer behavior can be challenging owing to the complex geometry of helical fuel rods. In this study, a general circumferentially nonuniform heat transfer fuel rod (GCNF) model is developed to predict the fuel central temperature and circumferential heat flux and wall temperature. This model incorporates a refined two-dimensional fuel conduction model and circumferential nonuniform shape factor, addressing the dual factors contributing to the circumferential nonuniformity of helical fuel rods. An empirical correlation for the nonuniform shape factor is developed based on the computational fluid dynamics (CFD) results, and it is implemented to the subchannel code. The newly developed model is applied to a helical fuel annulus and validated by comparing the prediction results with CFD data. The maximum wall temperature predicted by the code is 1.15°C lower than the value calculated through CFD. In terms of the heat flux, the maximum value at the inner corner is22 kW lower than that obtained from the CFD prediction. The accurate prediction of circumferentially nonuniform heat transfer in helical fuel, concerning the surface heat flux and cladding temperature, addresses existing shortcomings in helical fuel subchannel analysis methods. Additionally, the capability to predict the fuel central temperature is essential for the safety analysis to determine whether fuel rods are melting. The generality of the model framework allows it to be used for the prediction of circumferential nonuniform heat transfer behavior in other types of fuel assemblies.

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Laser assisted phase transformation of carbon-based transition metal oxide electrode for high-performance supercapacitor application

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Science China Chemistry 2025年

作者： Mingzhen xiu Jianghong zhang Yu Lu Xun Cao Kang Huang Siyu Zhu Bowei zhang Yu Liang Chunwang Zhao Junsheng Wu Yizhong Huang School of Materials Science and Engineering Nanyang Technological University Energy Research Institute Interdisciplinary Graduate Programme Nanyang Technological University Institute of Sustainability for Chemicals Energy and Environment Agency for Science Technology and ResearchSingapore Institute of Advanced Materials and Technology University of Science and Technology Beijing School of Materials Science and Technology Shenyang University of Chemical Technology School of Materials Science and Hydrogen Energy Foshan University

Transitional metal oxides have a wide range of applications due to their superior *** enhanced performance of metal oxide relies on their desirable oxidation states,which is challenging to achieve and requires complex *** study demonstrates a facile strategy enabling rapid phase transformation of transition metal *** approach involves synthesis of a tetragonal Mn3O4/carbon cloth composite using the liquid plasma discharge deposition （LPDD） technique,followed by laser irradiation with the presence of ethylene glycol under an N2gas *** resulting face-centered cubic MnO/carbon cloth electrode exhibits an excellent capacitance of 1,125 F g-1at 2 A g-1,with remarkable capacitance retention of 95%after10,000 *** approach can be extended to other transition metals including Co,Cu,Cr,Fe,Ni,and *** simulations reveal the significance of ethylene glycol and the underlying mechanism associated with the phase transformation of transition metal *** present study offers a powerful technique for the phase transformation of transition metal oxide which can guide the development of new electrode materials with enhanced electrochemical properties for high-performance energy storage applications.

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中国粮食安全和水稻生产

农业现代化研究 2005年第2期26卷 85-88页

作者：章秀福王丹英方福平曾衍坤廖西元中国水稻研究所浙江杭州310006

在分析水稻生产历史、粮食消费和国际贸易的基础上,指出水稻在我国粮食生产中具重要地位,水稻的稳定增产对保障国家食物安全起基础作用;在对国内外水稻单产水平进行比较的基础上,分析了我国水稻的生产潜力,提出水稻单产还有很大的提高空... 详细信息

在分析水稻生产历史、粮食消费和国际贸易的基础上,指出水稻在我国粮食生产中具重要地位,水稻的稳定增产对保障国家食物安全起基础作用;在对国内外水稻单产水平进行比较的基础上,分析了我国水稻的生产潜力,提出水稻单产还有很大的提高空间,可以满足我国未来对稻谷的需求;探讨了确保粮食安全的水稻发展对策。

关键词：粮食安全水稻生产发展对策

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting zhang Yanlin fu Peiyan Long Xiao Gao Zhengwei Wang Zhizhong Guan Xiaolan Qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK