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STUDY OF DERIVATIZATION OF PROTEIN WITH A EUROPIUM CHELATE AND APPLICATION TO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

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Chinese Chemical Letters 1993年第3期4卷 233-234页

作者： xiao da {2. Lan HUANG Qing Wei WANG {3. xiang ci Peking University,chemistry department,beijing 100871

A europium chelate of 5-chlorosulfoyl-2. thenoyltrifluoroacetone(CTTA)was investigated for precolumn derivatization of protein for high performance liquid *** new label was highly fluorescent and suitable for time-resolved fluorometric *** detective limit of protein is less than 0.3.g in sample with a volume of ***,the new label can also be used in gel electrophoresis and protein blotting.

关键词： high STUDY OF DERIVATIZATION OF PROTEIN WITH A EUROPIUM CHELATE AND APPLICATION TO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone

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Chinese Chemical Letters 1999年第6期10卷 495-498页

作者： Jun GAO Yuan Zong LI Zhen Quan GUO Wen Bac CHANG yun xiang {2.({3. of chemistry {3. of Biology. Peking University. beijing 100871) department of chemistry Peking University Beijing 100871 China department of Biology Peking University Beijing 100871 China

A novel immunoassay method for methyltestosterone (MT) in serum ovas *** antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was *** to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.

关键词： Methyltcstosterone immunoassay poly-N-isopropylacrylamide thermally reversible hydrogel

A FLUORESCENCE QUENCHING IMNUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED EUROPIUM CHELATE

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Chinese Chemical Letters 1992年第12期3卷 1011-1014页

作者： Feng Ji YAO Mei HONG xiao da {2. {3. xiang ci department of chemistry,Peking University,100871

A fluoroimmunoassay method using unlabeled europium chelate is *** principle is similar to that of fluoroimmunoassay method using lanthanide chelate as *** procedure is simple because labeling process is *** detection limit is about 10^(10) mol/L *** relative standard deviation of immunoassay is less than 10%.The recoveries of human serum albumin and estradiol protein conjugate are 96-105% and 111% respectively.

关键词： RSA Eu A FLUORESCENCE QUENCHING IMNUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED EUROPIUM CHELATE

CATALYTIC COLOR REACTION OF THE SYSTEM OF MIMETIC PEROXIdaSE(Fe-T(3.Py)P)-LEUCO CRYSTAL VIOLET-HYDROGEN PEROXIDE

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Chinese Chemical Letters 1992年第12期3卷 993-996页

作者： Shu Sheng SUN xiao Wei WU An WANG yun {2. {3. department of chemistry,Peking University,beijing,100871

The mimetic peroxidase iron-tetrakis(3.N-methylpyridyl)- porphyrin (Fe-T(3.Py)P)can be used as a catalyst in the spectrophoto- metric determination of H_2._2.and *** method is based on the formation of crystal violet from leuco crystal *** glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.

关键词： Fe-T CATALYTIC COLOR REACTION OF THE SYSTEM OF MIMETIC PEROXIdaSE LEUCO CRYSTAL VIOLET-HYDROGEN PEROXIDE

A QUENCHING FLUORESCENCE IMMUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED TERBIUM CHELATE

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Chinese Chemical Letters 1991年第9期2卷 737-738页

作者： Feng Ji YAO Pei Hong LI xiao da {2. Bo XING {3. xiang ci (department of chemistry,Peking University,100871)

A fluoroimmunoassay method using unlabeled Terbium chelate is *** principle is similar to that of fluoroimmunoassay method using lanthanide chelate as *** procedure is simpte because labeling process is *** recovery o... 详细信息

关键词： FIA A QUENCHING FLUORESCENCE IMMUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED TERBIUM CHELATE

STUDIES ON FLUORESCENCE ENHANciNG EFFECT AND ALKALINE DEGRAdaTION MECHANISM OF DOXYCYCLINE AND METHACYCLINE

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Chinese Chemical Letters 1993年第9期4卷 791-792页

作者： Yi Bing ZHAO department of chemistry,Xiamen University,Xiamen 3.1005Wen Bao CHANG {2. {3. ci department of chemistry,Peking University,beijing 100871

Degradation reaction of doxycycline or methacycline was carried out in KOH solution and intense fluorescence was obtained.A degradation mechanism of doxycycline or methacycline was suggested.

关键词： HC DC STUDIES ON FLUORESCENCE ENHANciNG EFFECT AND ALKALINE DEGRAdaTION MECHANISM OF DOXYCYCLINE AND METHACYCLINE KOH

STUDY　ON　THE　RELATIONSHIP　BETWEEN　MELTING　POINT　AND　AciD　DENATURING　AciDITY　OF　DNA　BY　ULTRAVIOLET　SPECTROMETER

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Chinese Chemical Letters 1994年第6期5卷 515-518页

作者： Yuan Zong LI xiang Dong LIU Wen Bao CHANG and yun xiang {3. (department of chemistry. Peking University, beijing 100871)

The relationship between meltillg point and acid denaturing acidity of DNA was studied by ultraviolet spectrometer. According to our investigation there exists a good linear relationship between melting point and acid denaturing acidity of DNA. The acid denaturing acidity. which could be obtained easily. may be used in the field of biochemistry as an alternative to melting point.

关键词： AciDITY DENATURING OF DNA AND

PRELIMINARY STUDIES ON THE IMMUNOCHEMICAL BEHAVIOR OF BSA-TTA-Eu CONJUGATES

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Chinese Chemical Letters 1994年第4期5卷 327-328页

作者： Wen Bac CHANG Shan WANG Nian Qing LU yun xiang {2.({3. of chemistry, Peking University, beijing 100871)Zhen Quan GUO({3. of Biology, Peking University, beijing 100871)

Thenoyltrifluoroacetone europium (TTA-Eu), as a hapten, was covalently attached to bovine serum albumin (BSA). The BSA-TTA-Eu conjugate was injected into rabbits to initiate anti-TTA-Eu sera. The sera were tested against TTA-Eu coupled to immunologically irrelative protein such as rabbit serum albumin (RSA) by an established enzyme-linked immunosorbent assay(ELISA). A cross-reaction test was performed to examine the specificity of the sera to recognize TTA-Eu and TTA. The preliminary results show that such sera have no detectable specificity to discriminate if an europium ion is coordinated to the TTA residue on the protein-TTA conjugate.

关键词： BSA TTA Eu

Causal relationship between feelings and cognitive decline:An univariable and multivariable Mendelian randomization study

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World Journal of Psychiatry 2024年第3期14卷 421-433页

作者： Juan Liu Lin Liu Yi-Xin Hu Jian-Hua Li xiao Zou Hao-yun Zhang Li Fan department of Cardiology The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China department of Pulmonary and Critical Care Medicine The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China The Fourth department of Geriatric Health Care The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China department of Anesthesiology First Medical Center of Chinese PLA General HospitalBeijing 100853China

BACKGROUND While the impact of depression on cognition is well-documented,the relationship between feelings and cognition has received limited *** To explore the potential association between feelings and cognition with a twosample Mendelian randomization(MR)*** Our analysis utilized genome-wide association {2.ta on various feelings(fed-up feelings,n=453.71;worrier/anxious feelings,n=450765;guilty feelings,n=45-0704;nervous feelings,n=450700;sensitivity/hurt feelings,n=449419;miserableness,n=454982.loneliness/isolation,n=4553.4;happiness,n=152.48)in the European population and their impact on cognitive functions(intelligence,n=2.9867).Conducting a univariable MR(UVMR)analysis to assess the relationship between feelings and *** this analysis,we applied the inverse variance weighting(IVW),weighted median,and MR Egger ***,we performed sensitivity analysis(leave-one-out analysis),assessed heterogeneity(using MR-PRESSO and Cochran’s Q test),and conducted multiple validity test(employing MR-Egger regression).Subsequently,a multivariable MR(MVMR)analysis was employed to examine the impact of feelings on *** served as the primary method in the multivariable analysis,complemented by median-based and MR-Egger *** In this study,UVMR indicated that sensitivity/hurt feelings may have a negative causal effect on cognition(OR=0.63.95%ci:0.43.0.92.P=0.017).After adjustment of other feelings using MVMR,a direct adverse causal effect on cognition was observed(OR_(MVMR)=0.3.,95%ci:0.17-0.90,P_(MVMR)=0.02.).While a potential increased risk of cognitive decline was observed for fed-up feelings in the UVMR analysis(ORUVMR=0.64,95%ci:0.42.0.97,PUVMR=0.03.),this effect disappeared after adjusting for other feelings(OR_(MVMR)=1.42.95%ci:0.43.4.74,P_(MVMR)=0.569).These findings were generally consistent across MV-IVW,median-based,and MR-Egger ***-Egger regression revealed pleiotropy in the impact of worrier/anxious

关键词： Mendelian randomization analysis Feelings Cognition Intelligence