DETERMINATION　OF　PROTEIN　USING　CATALYTIC　COLORIMETRY　OF　SULFANILIC　ACID　BY　COBALT（Ⅱ）

作     者：Yun Xiang CI Xiao Da YANG and Xiao Ting TANG(Department of Chemistry. Peking University. Beijing, 100871) 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：1994年第5卷第12期

页      面：1037-1038页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

主　　题：ACID SULFANILIC COBALT BY CATALYTIC 

摘      要：A new protein analysis method by the use of sulfanilic acid-hydrogen peroxide(H2O2)-Co2+ system was described. The method was based on the inhibition effect of protein on the catalytic activity of Co2+ for the coloration of sulfanilic acid. It took advantages of rather mild assay condition(pH 9.0), similar responses for albumin and globulin, and little interference. The assay could have a dynamic range of 0-1.00 mg/mL with a detection limit of about 0.01 mg/mL.