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Liposome Immunoassay for Determination of Phenytoin

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Chinese Chemical Letters 1998年第11期9卷 0-0,0-0页

作者： Wen Yu ZHU Wen Bao CHANG yun xiang ci (Department of Chemistry, Peking University, Beijing 100871) Department of Chemistry Peking University Beijing 100871 China

Homogeneous liposome immunoassay for the determination of phenytoin was *** were prepared from cholesterol (CH) and phospholipids including dipalmitoyl phosphatidyl ethanolamine (DPPE) tbr conjugation with thiol-containing antibodies. Hemin chloride was entrapped in the liposome and antibody was modified by reaction with 3' (2-pyndyl-dithio) propionyl N-hydroxysuccinimide ester (SPDP) to introduce thiol groups for efficient coupling. Antibody-coupled liposomes (immunoliposomes) were incubated with phenytoin and complement, and then with hemin substrate. The amount of hemin released from immunoliposomes, which increases with concentration increase of phenytoin, can be detected rapidly by determining the fluorescence with its substrate p-hydroxyphenyl propionic acid (HPPA)and hydrogen peroxide.

关键词： Iiposome phenytoin liposome immunoassay

The Electrochemical Impedance Behavior of the Living Cells Escherichia Coli

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Chinese Chemical Letters 1997年第12期8卷 0-0页

作者： Wei LI Jun FENG Hua Quan YANG and yun xiang ci(Department of Chemistry, Peking University, Beijing 100871) Department of Chemistry Peking University Beijing 100871 China

The semiconductive characteristics of clectron-transfrring proteins in living cells E coli was investigated by electrochemsical impedance spectroscopy(EIS). We found that the electrochemical impedance of living cells as a function of temprature followed the Arrhenius equation for semiconductors. This result shows a strong evidence to prove the semiconductive behavior of proteins

关键词： The Electrochemical Impedance Behavior of the Living Cells Escherichia Coli

STUDY　OF　THE　CHEMILUMINESCENCE　AND　FLUORESCENCE　REACTIONS　CATALYZED　BY　MIMETIC　PEROXIDASE　MN（II）－TETA

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Chinese Chemical Letters 1995年第12期6卷 1055-1056页

作者： Hui Zhi FAN yun xiang ci (Dept. of Chem., Peking Univ., Beijing, 100871) Beijing Univ Dept Chem Beijing 100871 Peoples R China

The mimeses of peroxidase by Mn(II)-TETA complex in catalytic luminol H2O2 chemiluminescence and L-Tyr-H2O2 fluorescence reactions were etudied The chemiluminescence and spectrofluorimetric methods of determination of H2O2 and glucose in human serum have been developed. The Michaelis-Menten constant K and maximum rate Vmax were measured.

关键词： II TETA AND

LABELING OF PROTEIN WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE(CTTA)AND APPLICATION TO IMMUNOASSAY

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Chinese Chemical Letters 1992年第12期3卷 1007-1010页

作者： yun xiang ci Xiao Da YANG Chemistry Department,Peking University,Beijing 100871

We describe for the first time the synthesis and the optimal conditions for protein labeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms a highly fluorescent conplex with Eu^(3+) when conjugated to *** labeled proteins were characterized by absorbance and fluorescence measurements and the effect of labeling on the biological activity of sone proteins was also *** is shown that the new label is suitable for applications in time-resolved fluoroimmunoassay.

关键词： Eu CTTA)AND APPLICATION TO IMMUNOASSAY LABELING OF PROTEIN WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE HSA

Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone

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Chinese Chemical Letters 1999年第6期10卷 495-498页

作者： Jun GAO Yuan Zong LI Zhen Quan GUO Wen Bac CHANG yun xiang ci(Department of Chemistry Department of Biology. Peking University. Beijing 100871) Department of Chemistry Department of Biology Peking University

A novel immunoassay method for methyltestosterone (MT) in serum ovas *** antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was *** to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.

关键词： Methyltcstosterone immunoassay poly-N-isopropylacrylamide thermally reversible hydrogel

STUDY　ON　THE　RELATIONSHIP　BETWEEN　MELTING　POINT　AND　AciD　DENATURING　AciDITY　OF　DNA　BY　ULTRAVIOLET　SPECTROMETER

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Chinese Chemical Letters 1994年第6期5卷 515-518页

作者： Yuan Zong LI xiang Dong LIU Wen Bao CHANG and yun xiang ci (Department of Chemistry. Peking University, Beijing 100871)

The relationship between meltillg point and acid denaturing acidity of DNA was studied by ultraviolet spectrometer. According to our investigation there exists a good linear relationship between melting point and acid denaturing acidity of DNA. The acid denaturing acidity. which could be obtained easily. may be used in the field of biochemistry as an alternative to melting point.

关键词： AciDITY DENATURING OF DNA AND

A FLUORESCENCE QUENCHING IMNUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED EUROPIUM CHELATE

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Chinese Chemical Letters 1992年第12期3卷 1011-1014页

作者： Feng Ji YAO Mei HONG Xiao Da YANG yun xiang ci Department of Chemistry,Peking University,100871

A fluoroimmunoassay method using unlabeled europium chelate is *** principle is similar to that of fluoroimmunoassay method using lanthanide chelate as *** procedure is simple because labeling process is *** detection limit is about 10^(10) mol/L *** relative standard deviation of immunoassay is less than 10%.The recoveries of human serum albumin and estradiol protein conjugate are 96-105% and 111% respectively.

关键词： RSA Eu A FLUORESCENCE QUENCHING IMNUNOASSAY METHOD FOR DETERMINATION OF TRACE ALBUMIN USING UNLABELED EUROPIUM CHELATE

A SENSITIVE PROTEIN ASSAY BY Co(II)-BIURET METHOD

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Chinese Chemical Letters 1994年第12期5卷 1039-1040页

作者： yun xiang ci Xiao Ting TANG and Xiao Da YANG (Department of Chemistry, Peking University, Beijing. 100871)

A new and senseitive Co (II)-biuret method for determination of protein has for the first time been established. In visible wavelength region, the Co (II) -protein complexes had anabsorption peak at 360 nm. -Under the present conditions. the assay had a linear range of 0-120 μg/ml of protein with a detection limit of about 2 μg/ml, and similar calibration curves were obtained for BSA. and BγG. Compared with Cu (II) -biuret method, the present Co (II) -biuret method was more simple, sensitive, and tolerant of interferences from non-protein molecules.

关键词： II A SENSITIVE PROTEIN ASSAY BY Co BIURET METHOD

CATALYTIC COLOR REACTION OF THE SYSTEM OF MIMETIC PEROXIDASE(Fe-T(3MPy)P)-LEUCO CRYSTAL VIOLET-HYDROGEN PEROXIDE

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Chinese Chemical Letters 1992年第12期3卷 993-996页

作者： Shu Sheng SUN Xiao Wei WU An WANG yun xiang ci Department of Chemistry,Peking University,Beijing,100871

The mimetic peroxidase iron-tetrakis(3-N-methylpyridyl)- porphyrin (Fe-T(3MPy)P)can be used as a catalyst in the spectrophoto- metric determination of H_2O_2 and *** method is based on the formation of crystal violet from leuco crystal *** glucose in human serum is determined by this reaction combined with catalytic reaction of glucose oxidase.

关键词： Fe-T CATALYTIC COLOR REACTION OF THE SYSTEM OF MIMETIC PEROXIDASE LEUCO CRYSTAL VIOLET-HYDROGEN PEROXIDE