Liposome Immunoassay for Determination of Phenytoin

作     者：Wen Yu ZHU Wen Bao CHANG Yun Xiang CI (Department of Chemistry, Peking University, Beijing 100871) 

作者机构：Department of Chemistry Peking University Beijing 100871 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：1998年第9卷第11期

页      面：0-0,0-0页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：Iiposome phenytoin liposome immunoassay 

摘      要：Homogeneous liposome immunoassay for the determination of phenytoin was *** were prepared from cholesterol (CH) and phospholipids including dipalmitoyl phosphatidyl ethanolamine (DPPE) tbr conjugation with thiol-containing antibodies. Hemin chloride was entrapped in the liposome and antibody was modified by reaction with 3 (2-pyndyl-dithio) propionyl N-hydroxysuccinimide ester (SPDP) to introduce thiol groups for efficient coupling. Antibody-coupled liposomes (immunoliposomes) were incubated with phenytoin and complement, and then with hemin substrate. The amount of hemin released from immunoliposomes, which increases with concentration increase of phenytoin, can be detected rapidly by determining the fluorescence with its substrate p-hydroxyphenyl propionic acid (HPPA)and hydrogen peroxide.