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pH-Responsive polymer boosts cytosolic siRNA release for retinal neovascularization therapy

Acta Pharmaceutica Sinica B 2024年第2期14卷 781-794页

作者： Shuai Guo Chunhui Li Changrong Wang Xiaowen Cao Xinyue Liu Xing-Jie Liang Yuanyu Huang yuhua weng School of Medical Technology Advanced Research Institute of Multidisciplinary ScienceSchool of Life ScienceKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China School of Pharmacy Shandong New Drug Loading&Release Technology and Preparation Engineering LaboratoryBinzhou Medical UniversityYantai 264003China School of Ophthalmology and Optometry School of Biomedical EngineeringThe Eye HospitalWenzhou Medical UniversityWenzhou 325027China Chinese Academy of Sciences(CAS) Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyCAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology of ChinaBeijing 100190China

Small interfering RNA(siRNA)has a promising future in the treatment of ocular diseases due to its high efficiency,specificity,and low toxicity in inhibiting the expression of target genes and ***,due to the unique anatomical structure of the eye and various barriers,delivering nucleic acids to the retina remains a significant *** this study,we rationally design PACD,an A-B-C type non-viral vector copolymer composed of a hydrophilic PEG block(A),a siRNA binding block(B)and a pH-responsive block(C).PACDs can self-assemble into nanosized polymeric micelles that compact siRNAs into polyplexes through simple *** evaluating its pH-responsive activity,gene silencing efficiency in retinal cells,intraocular distribution,and anti-angiogenesis therapy in a mouse model of hypoxia-induced angiogenesis,we demonstrate the efficiency and safety of PACD in delivering siRNA in the *** are surprised to discover that,the PACD/siRNA polyplexes exhibit remarkable intracellular endosomal escape efficiency,excellent gene silencing,and inhibit retinal *** study provides design guidance for developing efficient nonviral ocular nucleic acid delivery systems.

关键词： SiRNA Oculardeliverysystem Block polymer Endosomalescape Retinal neovascularization Blood-retinal barrier VEGFA Safety

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Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential ant1ip1 an-coronavirus activity

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1186-1198页

作者： Minghui Yang Chunhui Li Guoguo Ye Chenguang Shen Huiping Shi Liping Zhong Yuxin Tian Mengyuan Zhao Pengfei Wu Abid Hussain Tian Zhang Haiyin Yang Jun Yang yuhua weng Xinyue Liu Zhimin Wang Lu Gan Qianyu Zhang Yingxia Liu Ge Yang Yuanyu Huang Yongxiang Zhao School of Life Science Advanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijing 100081China National Clinical Research Center for infectious disease Shenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China Guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Targeting Oncology National Center for International Research of Biotargeting TheranosticsGuangxi Key Laboratory of Biotargeting TheranosticsCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Medical UniversityNanning 530021China CAMS Key Laboratory of Antiviral Drug Research Beijing Key Laboratory of Antimicrobial AgentsInstitute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Emerging and recurrent infectious diseases caused by human coronaviruses(HCoVs)continue to pose a significant threat to global public health *** light of this ongoing threat,the development of a broad-spectrum drug to combat HCoVs is an urgently ***,we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment(SELEX).These aptamers have nanomolar affinity with the nucleocapsid protein(NP)of Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and also show excellent binding efficiency to the N proteins of both SARS,MERS,HCoV-OC43 and-NL63 with affinity KD values of 1.31 to 135.36 *** aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant(BA.5)with EC50 values at 2.00 nM and 41.08 nM,*** protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs(−229E and-HKU1).In conclusion,we have identified six aptamers with a high pan-coronavirus antiviral activity,which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.

关键词： acute threat conclusion

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Biosafety materials:Ushering in a new era of infectious disease diagnosis and treatment with the CRISPR/Cas system

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Biosafety and Health 2022年第2期4卷 70-78页

作者： Yuquan Zhang Ziyue Li Julien Milon Essola Kun Ge Xuyan Dai Huining He Haihua Xiao yuhua weng Yuanyu Huang School of Life Science School of Medical TechnologyAdvanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China Key Laboratory of Analytical Science and Technology of Hebei Province College of Chemistry and Environmental ScienceKey Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of EducationHebei UniversityBaoding 071000China Hunan Agricultural University Changsha 410128China Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics School of PharmacyTianjin Medical UniversityTianjin 300070China Beijing National Laboratory for Molecular Science State Key Laboratory of Polymer Physical and ChemistryInstitute of ChemistryChinese Academy of ScienceBeijing 100190China School of Materials and the Environment Beijing Institute of TechnologyZhuhai 519085China

Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on global *** clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system has the potential to address these critical needs for tackling infectious diseases to detect viral nucleic acids and inhibit viral *** review summarizes how the CRISPR/Cas system is being utilized for the treatment and diagnosis of infectious diseases with the help of biosafety materials and highlights the design principle and in vivo and in vitro efficacy of advanced biosafety materials used to deal with virus attacks.

关键词： Biosafety materials CRISPR/Cas SARS-CoV-2 Diagnosis Infectious disease

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Advances of mRNA vaccines for COVID-19:A new prophylactic revolution begins

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Asian Journal of Pharmaceutical Sciences 2021年第3期16卷 263-264页

作者： yuhua weng Yuanyu Huang Institute of Engineering Medicine Advanced Research Institute of Multidisciplinary ScienceSchool of Life ScienceKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijing 100081China

The world is in the midst of 2019 coronavirus infection disease(COVID-19)*** of 30 January 2021,more than 100 million cases and 2.1 million deaths were confirmed according to the data from the World Health Organization(WHO),resulting in a widespread social and economic ***,researches worldwide are racing to deploy safe and effective COVID-19 vaccines.

关键词： vaccines infection cases

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Ionizable lipid-assisted efficient hepatic delivery of gene editing elements for oncotherapy

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Bioactive Materials 2022年第3期7卷 590-601页

作者： Chunhui Li Tongren Yang yuhua weng Mengjie Zhang Deyao Zhao Shuai Guo Bo Hu Wanxuan Shao Xiaoxia Wang Abid Hussain Xing-Jie Liang Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceInstitute of Engineering MedicineKey Laboratory of Molecular Medicine and BiotherapyBeijing Institute of TechnologyBeijing100081China Department of Radiation Oncology The First Affiliated Hospital of Zhengzhou UniversityErqiZhengzhou450000China Institute of Molecular Medicine College of Future TechnologyPeking UniversityBeijing100871China Chinese Academy of Sciences(CAS)Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology of ChinaBeijing100190China School of Materials and the Environment Beijing Institute of TechnologyZhuhai519085China

CRISPR/Cas9-based gene editing has emerged as a powerful biotechnological tool,that relies on Cas9 protein and single guided RNA(sgRNA)to edit target ***,the lack of safe and efficient delivery carrier is one of the crucial factors restricting its clinical ***,we report an ionizable lipid nanoparticle(iLP181,pKa=6.43)based on iLY1809 lipid enabling robust gene editing in vitro and in *** iLP181 effectively encapsulate psgPLK1,the best-performing plasmid expressing for both Cas9 protein and sgRNA targeting Polo-like kinase 1(PLK1).The iLP181/psgPLK1 nanoformulation showed uniformity in size,regular nanostructure and nearly neutral zeta potential at pH *** nanoformulation effectively triggered editing of PLK1 gene with more than 30%efficiency in HepG2-Luc ***181/psgPLK1 significantly accumulated in the tumor for more than 5 days after a single intravenous *** addition,it also achieved excellent tumor growth suppression compared to other nucleic acid modalities such as siRNA,without inducing adverse effects to the main organs including the liver and *** study not only provides a clinically-applicable lipid nanocarrier for delivering CRISPR/Cas system(even other bioactive molecules),but also constitutes a potential cancer treatment regimen base on DNA editing of oncogenes.

关键词： CRISPR/Cas Gene editing Lipid nanoparticle PLK1 Cancer therapy

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Nanotechnology-based strategies for treatment of ocular disease

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Acta Pharmaceutica Sinica B 2017年第3期7卷 281-291页

作者： yuhua weng Juan Liu Shubin Jin Weisheng Guo Xingjie Liang Zhongbo Hu College of Materials Science and Opto-Electronic Technology University of Chinese Academy of Sciences Laboratory of Controllable Nanopharmaceuticals Chinese Academy of Sciences(CAS)Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology of China University of Chinese Academy of Sciences

Ocular diseases include various anterior and posterior segment diseases. Due to the unique anatomy and physiology of the eye, efficient ocular drug delivery is a great challenge to researchers and pharmacologists. Although there are conventional noninvasive and invasive treatments, such as eye drops,injections and implants, the current treatments either suffer from low bioavailability or severe adverse ocular effects. Alternatively, the emerging nanoscience and nanotechnology are playing an important role in the development of novel strategies for ocular disease therapy. Various active molecules have been designed to associate with nanocarriers to overcome ocular barriers and intimately interact with specific ocular tissues. In this review, we highlight the recent attempts of nanotechnology-based systems for imaging and treating ocular diseases, such as corneal d iseases, glaucoma, retina diseases, and choroid diseases. Although additional work remains, the progress described herein may pave the way to new,highly effective and important ocular nanomedicines.

关键词： Nanosystems Nanocarrier Eye Ocular disease Ocular drug delivery Therapy Diagnosis

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A Dual-Task Learning Approach for Bearing Anomaly Detection and State Evaluation of Safe Region

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 229-241页

作者： yuhua Yin Zhiliang Liu Bin Guo Mingjian Zuo School of Mechanical and Electrical Engineering University of Electronic Science and Technology of ChinaChengdu 611731China Qingdao International Academician Park Research Institute Qingdao 266041China

Predictive maintenance has emerged as an effective tool for curbing maintenance costs,yet prevailing research predominantly concentrates on the abnormal *** the ostensibly stable healthy phase,the reliance on anomaly detection to preempt equipment malfunctions faces the challenge of sudden anomaly *** address this challenge,this paper proposes a dual-task learning approach for bearing anomaly detection and state evaluation of safe *** proposed method transforms the execution of the two tasks into an optimization issue of the hypersphere *** leveraging the monotonicity and distinguishability pertinent to the tasks as the foundation for optimization,it reconstructs the SVDD model to ensure equilibrium in the model’s performance across the two *** experiments verify the proposed method’s effectiveness,which is interpreted from the perspectives of parameter adjustment and enveloping *** the meantime,experimental results also show two deficiencies in anomaly detection accuracy and state evaluation *** theoretical analysis inspires us to focus on feature extraction and data collection to achieve *** proposed method lays the foundation for realizing predictive maintenance in a healthy stage by improving condition awareness in safe regions.

关键词： Bearing condition monitoring Anomaly detection Safe region Support vector data description

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A novel polyethyleneimine-decorated FeOOH nanoparticle for efficient siRNA delivery

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Chinese Chemical Letters 2021年第1期32卷 102-106页

作者： Shuai Guo Bei Liu Mengjie Zhang Chunhui Li Xiaoxia Wang yuhua weng Lele Li Yuanyu Huang School of Life Science Advanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyInstitute of Engineering MedicineBeijing Institute of TechnologyBeijing 100081China CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology(NCNST)Beijing 100190China School of Pharmacy Hunan University of Chinese MedicineChangsha 410208China College of Materials Science and Optoelectronic Technology University of Chinese Academy of SciencesBeijing 100049China Hunan University of Science and Engineering Yongzhou 425199China Institute of Molecular Medicine Peking UniversityBeijing 100871China

Despite the promising prospect of small interfering RNA(siRNA) for the treatment of diverse diseases,it remains challenging to develop novel delive ry materials to desired tissues and *** this study,a novel iron oxyhydroxide(FeOOH) nanoparticle(NP) whose surface was modified with branched polyetherimide(PEI) was developed to deliver siRNA into the cancer *** was demonstrated that PEI-FeOOH(PFeOOH) efficiently complexed siRNA,mediated effective cellular uptake and endosomal escape,thereby triggering robust gene silencing in *** addition,PFeOOH/siRNA formulation loading with anti-RRM2 siRNA effectively inhibited the growth of tumor tissues,and exhibited excellent safety profiles in ***,this study conceptually provided a FeOOH-based nucleic acid delivery vesicle which can potentially use to achieve diagnosis and therapy simultaneously.

关键词： FeOOH siRNA delivery RRM2 RNA interference Polyetherimide Cancer treatment

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MXene Key Composites:A New Arena for Gas Sensors

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Nano-Micro Letters 2024年第10期16卷 350-391页

作者： Yitong Wang yuhua Wang Min Jian Qinting Jiang Xifei Li Hubei Province Key Laboratory of Systems Science in Metallurgical Process Wuhan University of Science and TechnologyWuhan 430081People's Republic of China Key Materials and Components of Electrical Vehicles for Overseas Expertise Introduction Center for Discipline Innovation Institute of Advanced Electrochemical Energy and School of Materials Science and EngineeringXi'an University of TechnologyXi'an 710048People's Republic of China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108FujianPeople's Republic of China

With the development of science and technology,the scale of industrial production continues to grow,and the types and quantities of gas raw materials used in industrial production and produced during the production process are also constantly *** gases include flammable and explosive gases,and even contain toxic ***,it is very important and necessary for gas sensors to detect and monitor these gases quickly and *** recent years,a new two-dimensional material called MXene has attracted widespread attention in various *** abundant surface functional groups and sites,excellent current conductivity,tunable surface chemistry,and outstanding stability make them promising for gas sensor *** the birth of MXene materials,researchers have utilized the efficient and convenient solution etching preparation,high flexibility,and easily functionalize MXene with other materials to pre-pare composites for gas *** has opened a new chapter in high-performance gas sensing materials and provided a new approach for advanced sensor ***,previous reviews on MXene-based composite materials in gas sensing only focused on the performance of gas sensing,without systematically explaining the gas sensing mechanisms generated by different gases,as well as summarizing and predicting the advantages and disadvantages of MXene-based composite *** article reviews the latest progress in the application of MXene-based composite materials in gas ***,a brief summary was given of the commonly used methods for preparing gas sens-ing device structures,followed by an introduction to the key attributes of MXene related to gas sensing *** article focuses on the performance of MXene-based composite materials used for gas sensing,such as MXene/graphene,MXene/Metal oxide,MXene/Transition metal sulfides(TMDs),MXene/Metal-organic framework(MOF),MXene/*** summarizes the advantages and disad

关键词： MXene Compound material Gas sensor Gas sensitive preparation Gas sensitivity performance

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Atomically dispersed Ni electrocatalyst for superior urea-assisted water splitting

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Journal of Energy Chemistry 2024年第3期90卷 1-6,I0002页

作者： Fang Luo Shuyuan Pan yuhua Xie Chen Li Yingjie Yu Zehui Yang College of Materials Science and Engineering State Key Laboratory of New Textile Materials&Advanced Processing TechnologyWuhan Textile UniversityWuhan 430200China Hubei Hydrogen Energy Technology Innovation Center Faculty of Materials Science and ChemistryChina University of GeosciencesWuhan 430074HubeiChina

Urea oxidation reaction(UOR) has been selected as substitution for oxygen evolution reaction ascribing to its low thermodynamic voltage as well as utilization of nickel as ***,we report the formation of nickel single atoms(Ni-SAs) as exceptional bifunctional electrocatalyst toward UOR and hydrogen evolution reaction(HER) in urea-assisted water *** UOR catalysis,Ni-SAs perform a superior catalytic performance than Ni-NP/NC and Pt/C ascribing to the formation of HOO-Ni-N_(4) structure evidenced by in-situ Raman spectroscopy,corresponding to a boosted mass activity by 175-fold at 1.4 V *** than Ni-NP/***,Ni-SAs requires only 450 mV overpotential to obtain HER current density of 500 mA cm^(-2).136 mA cm^(-2) is achieved in urea-assisted water splitting at1.7 V for Ni-SAs,boosted by 5.7 times than Pt/C-IrO_(2) driven water splitting.

关键词： Urea oxidation reaction Hydrogen evolution reaction Nickel single atoms Water splitting

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