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Science Bulletin 2015年第14期60卷 1241-1251页

作者： Pin Liu Hong Ji Chao Li Li-Qiao Chen zhen-yu du College of Animal Science and Technology Northwest A&F University Station of China Fisheries Law Enforcement School of Life Sciences East China Normal University

To investigate the differentiation mechanism of grass carp preadipocytes, a primary adipocytes culture system was established. Confluent preadipocytes were induced to differentiation, and the morphology and gene expression were evaluated at different stages. It was shown that preadipocytes were gradually filled with droplets and the cellular lipid content increased during the differentiation. Ultrastructure observation indicated that the number of mitochondria increased with adipocytes differentiation. Consistently, the mitochondrial protein content was ele- vated in the differentiating adipocytes, qRT-PCR showed that the expression level of lipogenesis-related genes such as peroxisome proliferator activator receptor 7 （PPAR 7）, lipoprotein lipase （LPL）, fatty acid synthase （FAS） and stearoyl-CoA desaturase （SCD） increased during adipocytes differentiation. The mitochondrial relevant gene also elevated when adipocyte differentiation, such as PPAR coactivator-1 （PGC-1 α）, PGC-1β and nuclear respiratory factor （NRF-1）. However, the expression of carnitine palmitoyltransferase 1α （CPT-1 α） gene decreased at the initial stage, but increased at the last stage of cell differ- entiation. These results indicated that the differentiation process of grass carp preadipocytes is similar to that of land animals, but the molecular mechanisms are not exactly the same. The findings revealed in this study provides new information to the study of fish adipocyte differentiation.

关键词： Grass carp. Adipocytes. DifferentiationMitochondria

Double-edged effect of sodium citrate in Nile tilapia(Oreochromis niloticus):Promoting lipid and protein deposition *** hyperglycemia and insulin resistance

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Animal Nutrition 2023年第3期14卷 303-314页

作者： Jun-Xian Wang Fang Qiao Mei-Ling Zhang Li-Qiao Chen zhen-yu du yuan Luo Laboratory of Aquaculture Nutrition and Environmental Health(LANEH) School of Life SciencesEast China Normal UniversityShanghaiChina

Citrate is an essential substrate for energy metabolism that plays critical roles in regulating glucose and lipid metabolic ***,the action of citrate in regulating nutrient metabolism in fish remains poorly ***,we investigated the effects of dietary sodium citrate on growth performance and systematic energy metabolism in juvenile Nile tilapia(Oreochromis niloticus).A total of 270Nile tilapia(2.81±0.01 g)were randomly divided into three groups(3 replicates per group,30 fish per replicate)and fed with control diet(35%protein and 6%lipid),2%and 4%sodium citrate diets,respectively,for 8 *** results showed that sodium citrate exhibited no effect on growth performance(P>0.05).The whole-body crude protein,serum triglyceride and hepatic glycogen contents were significantly increased in the 4%sodium citrate group(P0.05).The 4%sodium citrate treatment significantly increased the serum glucose and insulin levels at the end of feeding trial and also in the glucose tolerance test(Pduced crude protein deposition in muscle by activating m TOR signaling and inhibiting AMPK signaling(P<0.05).Furthermore,the 4%sodium citrate significantly suppressed serum aspartate aminotransferase and alanine aminotransferase activities,along with the lowered expression of pro-inflammatory genes,such as nfκb,tnfa and il8(P<0.05).Although the 4%sodium citrate significantly increased phosphor-nuclear factor-k B p65 prote

关键词： Sodium citrate Lipid and protein deposition Insulin resistance Nile tilapia

Inhibition of pyruvate dehydrogenase kinase improves carbohydrate utilization in Nile tilapia by regulating PDK2/4-PDHE1αaxis and insulin sensitivity

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Animal Nutrition 2022年第4期11卷 25-37页

作者： yuan Luo Wenhao Zhou Ruixin Li Samwel MLimbu Fang Qiao Liqiao Chen Meiling Zhang zhen-yu du LANEH School of Life SciencesEast China Normal UniversityShanghai 200241China University of Dar Es Salaam Department of Aquaculture TechnologyDar Es Salaam 60091Tanzania UDSM-ECNU Joint Research Center for Aquaculture and Fish Biology(JRCAFB) Dar Es Salaam 60091Tanzania ECNU-UDSM Joint Research Center for Aquaculture and Fish Biology(JRCAFB) Shanghai 200241China

Pyruvate dehydrogenase kinases(PDKs)-pyruvate dehydrogenase E1αsubunit(PDHE1α)axis plays an important role in regulating glucose metabolism in ***,the regulatory function of PDKsPDHE1α axis in the glucose metabolism of fish is not well *** study determined whether PDKs inhibition could enhance PDHE1αactivity,and improve glucose catabolism in *** tilapia fingerlings(1.90±0.11 g)were randomly divided into 4 treatments in triplicate(30 fish each)and fed control diet without dichloroacetate(DCA)(38% protein,7% lipid and 45% corn starch)and the control diet supplemented with DCA,which inhibits PDKs through binding the allosteric sites,at 3.75(DCA3.75),7.50(DCA7.50)and 11.25 g/kg(DCA11.25),for 6 *** results showed that DCA3.75,DCA7.50 and DCA11.25significantly increased weight gain,carcass ratio and protein efficiency ratio(Pduced feed efficiency(Pduced the mesenteric fat index,serum and liver triglyceride concentration and total lipid content in whole fish,and down-regulated the expressions of genes related to lipogenesis(Pduced the conversion of glucose to lipid(P<0.05).Furthermore,the DCA3.75 treatment significantly decreased the PDK2/4 gene and protein expressions(P<0.05),accordingly stimulated PDHE1αactivity by decreasing the phosphorylated PDHE1αprotein *** addition,DCA3.75 treatment significantly increased the phosphorylated levels of key proteins involved in insulin signaling pathway and glycogen synthase kinase 3β(P<0.05).Taken together,the present study demonstrates that PDK2/4 inhibition by using DCA promotes glucose utilization in Nile tilapia by activating PDHE1αan

关键词： Dichloroacetate Glucose utilization Insulin sensitivity Nile tilapia PDK2/4-PDHE1αaxis

Partial replacement of soybean meal by yellow mealworm(Tenebrio molitor)meal influences the flesh quality of Nile tilapia(Oreochromis niloticus)

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Animal Nutrition 2023年第1期12卷 108-115页

作者： Le Zhang Hong-Xia Wu Wei-Jie Li Fang Qiao Wen-Bing Zhang zhen-yu du Mei-Ling Zhang Laboratory of Aquaculture Nutrition and Environmental Health School of Life SciencesEast China Normal UniversityShanghai 200241China The Key Laboratory of Mariculture Ministry of EducationThe Key Laboratory of Aquaculture Nutrition and FeedsMinistry of AgricultureOcean University of ChinaQingdao 266003China

This study investigated the effects of yellow mealworm meal(YM)replacing soybean meal(SBM)at different proportions(0%,15%,30%and 45%,referred as YM0,YM15,YM30 and YM45,respectively)on the flesh quality of Nile tilapia.A total of 360 fish(70.0±0.12 g)were randomly divided into 4 groups(3 tanks per group).Fish were fed the experimental diet twice daily for 10 *** results showed that muscle protein content significantly decreased in YM30 and YM45,while the lipid content significantly decreased in YM45(P<0.05).The essential amino acids and flavor amino acids of the muscle were not affected by the YM substitution,while saturated fatty acid content decreased in YM30 and YM45 compared with YM0(P<0.05).Fillets in YM45 had higher hardness,gumminess,and a higher proportion of thin myofibers(≤100μm,P<0.05)than those in other *** analysis revealed that apoptosis and atrophy related genes were up-regulated,while the muscle antioxidant capacity decreased significantly in YM45(P<0.05),which may be related to the high acid value in YM45 *** findings indicated that YM could replace up to 30%SBM without substantially altering the flesh *** the replacement ratio increased to 45%,the flesh quality would *** attention should be paid to avoid feed rancidity which may affect the flesh quality of fish.

关键词： Yellow mealworm meal Novel feed protein source Nile tilapia Flesh quality Texture property

Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates

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动物学研究(英文) 2023年第5期44卷 954-966页

作者： Qiang Ma yuan Luo Jia Zhong Samwel Mchele Limbu Ling-yu Li Li-Qiao Chen Fang Qiao Mei-Ling Zhang Qiang Lin zhen-yu du LANEH School of Life SciencesEast China Normal UniversityShanghai 200241China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdaoShandong 266071China CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouGuangdong 510301China School of Aquatic Sciences and Fisheries Technology University of Dar es SalaamDar es Salaam 60091Tanzania

Hypoxia is a common environmental stress factor in aquatic organisms,which varies among fish ***,the mechanisms underlying the ability of fish species to tolerate hypoxia are not well ***,we showed that hypoxia response in different fish species was affected by lipid catabolism and preference for lipid or carbohydrate energy *** of biochemical lipid catabolism through peroxisome proliferator-activated receptor alpha(Pparα)or increasing mitochondrial fat oxidation in tilapia decreased tolerance to acute hypoxia by increasing oxygen consumption and oxidative damage and reducing carbohydrate catabolism as an energy ***,lipid catabolism inhibition by suppressing entry of lipids into mitochondria in tilapia or individually knocking out three key genes of lipid catabolism in zebrafish increased tolerance to acute hypoxia by decreasing oxygen consumption and oxidative damage and promoting carbohydrate ***,anaerobic glycolysis suppression eliminated lipid catabolism inhibition-promoted hypoxia tolerance in adipose triglyceride lipase(atgl)mutant *** 14 fish species with different trophic levels and taxonomic status,the fish preferentially using lipids for energy were more intolerant to acute hypoxia than those preferentially using *** study shows that hypoxia tolerance in fish depends on catabolic preference for lipids or carbohydrates,which can be modified by regulating lipid catabolism.

关键词： Hypoxia tolerance Catabolic preference Lipid Carbohydrate Oxidative damage

Uridine alleviates high-carbohydrate diet-induced metabolic syndromes by activating sirt1/AMPK signaling pathway and promoting glycogen synthesis in Nile tilapia(Oreochromis niloticus)

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Animal Nutrition 2023年第3期14卷 56-66页

作者： Nan-Nan Zhou Tong Wang yu-Xin Lin Rong Xu Hong-Xia Wu Fei-Fei Ding Fang Qiao zhen-yu du Mei-Ling Zhang Laboratory of Aquaculture Nutrition and Environmental Health(LANEH) School of Life SciencesEast China Normal UniversityShanghai 200241China

Carbohydrates have a protein sparing effect,but long-term feeding of a high-carbohydrate diet(HCD)leads to metabolic disorders due to the limited utilization efficiency of carbohydrates in *** to mitigate the negative effects induced by HCD is crucial for the rapid development of *** is a pyrimidine nucleoside that plays a vital role in regulating lipid and glucose metabolism,but whether uridine can alleviate metabolic syndromes induced by HCD remains *** this study,a total of480 Nile tilapia(Oreochromis niloticus)(average initial weight 5.02±0.03 g)were fed with 4 diets,including a control diet(CON),HCD,HCD+500 mg/kg uridine(HCUL)and HCD+5,000 mg/kg uridine(HCUH),for 8 *** results showed that addition of uridine decreased hepatic lipid,serum glucose,triglyceride and cholesterol(Pduced metabolic syndrome by activating the sirt1/AMPK signaling pathway and promoting glycogen *** finding reveals the function of uridine in fish metabolism and facilitates the development of new additives in aquatic feeds.

关键词： Uridine High-carbohydrate diet Metabolism AMPK Nile tilapia

Lipophagy is essential for lipid metabolism in fish

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Science Bulletin 2018年第14期63卷 879-882页

作者： Jing wang Si-Lan Han Ling-yu Li Dong-Liang Lu Samwel Mchele Limbu Dong-Liang Li Mei-Ling Zhang zhen-yu du Laboratory of Aquaculture Nutrition and Environmental Health(LANEH) School of Life SciencesEast China Normal University Shanghai 200241. China Department of Aquatic Sciences and Fisheries Technology University of Dar es SalaamDares Salaam Tanzania

Lipophagy is a form of autophagy in *** this cellular process,lipid droplets(LDs)are degraded through the lysosomal degradative pathway[1].during lipophagy,LDs sequestrated in autophagosomes are delivered to lysosomes,which fuse to form autophagosomes and then develop into autophagolysosomes,leading to LDs degradation of[1].A number of studies have demonstrated that lipophagy play important roles in lipid

关键词：类脂化合物新陈代谢鱼哺乳动物生物功能脊椎动物 ing LD

High cholesterol intake remodels cholesterol turnover and energy homeostasis in Nile tilapia(Oreochromis niloticus)

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Marine Life Science & Technology 2023年第1期5卷 56-74页

作者： Rui-Xin Li Ling-yun Chen Samwel M.Limbu yu-Cheng Qian Wen-Hao Zhou Li-Qiao Chen yuan Luo Fang Qiao Mei-Ling Zhang zhen-yu du LANEH School of Life SciencesEast China Normal UniversityShanghai200241China Department of Aquaculture Technology School of Aquatic Sciences and Fisheries TechnologyUniversity of Dar es SalaamP.O.Box 60091Dar es SalaamTanzania

The roles of dietary cholesterol in fish physiology are currently *** issue reflects the limited studies on the metabolic consequences of cholesterol intake in *** present study investigated the metabolic responses to high cholesterol intake in Nile tilapia(Oreochromis niloticus),which were fed with four cholesterol-contained diets(0.8,1.6,2.4 and 3.2%)and a control diet for eight *** fish-fed cholesterol diets showed increased body weight,but accumulated cholesterol(the peak level was in the 1.6%cholesterol group).Then,we selected 1.6%cholesterol and control diets for further *** high cholesterol diet impaired liver function and reduced mitochondria number in ***,high cholesterol intake triggered protective adaptation via(1)inhibiting endogenous cholesterol synthesis,(2)elevating the expression of genes related to cholesterol esterification and efflux,and(3)promoting chenodeoxycholic acid synthesis and ***,high cholesterol intake reshaped the fish gut microbiome by increasing the abundance of Lactobacillus *** Mycobacterium spp.,both of which are involved in cholesterol and/or bile acids ***,high cholesterol intake inhibited lipid catabolic activities through mitochondrialβ-oxidation,and lysosome-mediated lipophagy,and depressed insulin signaling *** catabolism was elevated as a compulsory response to maintain energy ***,although high cholesterol intake promoted growth,it led to metabolic disorders in *** the first time,this study provides evidence for the systemic metabolic response to high cholesterol intake in *** knowledge contributes to an understanding of the metabolic syndromes caused by high cholesterol intake or deposition in fish.

关键词： Cholesterol metabolism Metabolic response Energy metabolism Gut microbiome

Recent progress in self-propelled particles

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Journal of Hydrodynamics 2024年第1期36卷 61-77页

作者： zhen-yu Ouyang Jian-zhong Lin Laboratory of Impact and Safety Engineering Ministry of EducationNingbo UniversityNingbo315201China Department of Mechanics State Key Laboratory of Fluid Power and Mechatronic SystemsZhejiang UniversityHangzhou310027China

Self-propelled particles are commonly found in a large number of planktonic organisms such as bacteria,fungi,and algae in nature,and researchers have taken a long interest in exploring their swimming mechanisms for more than a *** in the past 20 years,with the development of computational fluid dynamics and flow display technology,as well as the need for the design of synthetic self-propelled particles and micro-swimming devices,self-propelled particles have become the forefront and hotspot of current research in the field of fluid *** paper first introduces the swimming characteristics of common self-propelled particles,leading to a classic“squirmer”type self-propelled particle *** this basis,a systematic introduction and summary of the theoretical and numerical simulation research of“squirmer”will be ***,the main challenges and opportunities faced by the current research will be summarized.

关键词： Self-propelled particles multiphase flow hydrodynamic interaction

Design and Gait Planning of a Worm-inspired Metameric Robot for Pipe Crawling

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Journal of Bionic Engineering 2024年第3期21卷 1265-1277页

作者： yu Liu Qingbiao Shi zhen Chen Pen-Tung Sah Institute of Micro-Nano Science and Technology Xiamen UniversityXiamen361102China

The earthworm has been attracted much attention in the research and development of biomimetic robots due to their unique locomotion mechanism,compact structure,and small motion *** paper presents a new design and prototype of a worm-inspired metameric robot with a movement pattern similar to that of *** robot consists of multiple telescopic modules connected in series through joint *** telescopic module mimics the contraction and elongation motion modes of the earthworm segments.A kinematic and dynamic analysis is conducted on the telescopic module,and an input torque calculation method is provided to ensure sufficient friction between the robot and the pipe *** gait modes of the prototype robot for straight and turning locomotion are introduced,and these modes are extended to robots constructed by different numbers of telescopic *** addition,a method is proposed to increase the friction between the robot and the pipe wall in the aforementioned gait modes without changing the robot structure,thereby improving the robot’s motion ability in *** theoretical model of gait modes has also been validated through gait *** findings of this paper would provide a useful basis for the design,modeling,and control of future worm inspired robots.

关键词： Bio-inspired robot Spatial linkage mechanism Kinematics model Peristaltic locomotion