Uridine alleviates high-carbohydrate diet-induced metabolic syndromes by activating sirt1/AMPK signaling pathway and promoting glycogen synthesis in Nile tilapia(Oreochromis niloticus)

作     者：Nan-Nan Zhou Tong Wang Yu-Xin Lin Rong Xu Hong-Xia Wu Fei-Fei Ding Fang Qiao Zhen-Yu Du Mei-Ling Zhang Nan-Nan Zhou;Tong Wang;Yu-Xin Lin;Rong Xu;Hong-Xia Wu;Fei-Fei Ding;Fang Qiao;Zhen-Yu Du;Mei-Ling Zhang

作者机构：Laboratory of Aquaculture Nutrition and Environmental Health(LANEH)School of Life SciencesEast China Normal UniversityShanghai 200241China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第14卷第3期

页      面：56-66页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：financial support provided by the National Key Research and Development Program(grant number:2022YFD2400800) National Natural Science Foundation of China(grant number:31972798) 

主　　题：Uridine High-carbohydrate diet Metabolism AMPK Nile tilapia 

摘      要：Carbohydrates have a protein sparing effect,but long-term feeding of a high-carbohydrate diet(HCD)leads to metabolic disorders due to the limited utilization efficiency of carbohydrates in *** to mitigate the negative effects induced by HCD is crucial for the rapid development of *** is a pyrimidine nucleoside that plays a vital role in regulating lipid and glucose metabolism,but whether uridine can alleviate metabolic syndromes induced by HCD remains *** this study,a total of480 Nile tilapia(Oreochromis niloticus)(average initial weight 5.02±0.03 g)were fed with 4 diets,including a control diet(CON),HCD,HCD+500 mg/kg uridine(HCUL)and HCD+5,000 mg/kg uridine(HCUH),for 8 *** results showed that addition of uridine decreased hepatic lipid,serum glucose,triglyceride and cholesterol(P0.05).Further analysis indicated that higher concentration of uridine activated the sirtuin1(sirt1)/adenosine 5-monophosphate-activated protein kinase(AMPK)signaling pathway to increase lipid catabolism and glycolysis while decreasing lipogenesis(P0.05).Besides,uridine increased the activity of glycogen synthesis-related enzymes(P0.05).This study suggested that uridine could alleviate HCD-induced metabolic syndrome by activating the sirt1/AMPK signaling pathway and promoting glycogen *** finding reveals the function of uridine in fish metabolism and facilitates the development of new additives in aquatic feeds.