Double-edged effect of sodium citrate in Nile tilapia(Oreochromis niloticus):Promoting lipid and protein deposition *** hyperglycemia and insulin resistance
作者机构：Laboratory of Aquaculture Nutrition and Environmental Health(LANEH)School of Life SciencesEast China Normal UniversityShanghaiChina
出 版 物：《Animal Nutrition》 (动物营养（英文版）)
年 卷 期：2023年第14卷第3期
页 面：303-314页
核心收录：
学科分类：1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 100214[医学-肿瘤学] 10[医学]
基 金：support provided by National Key Research and Development Program of China China(2018YFD0900400)
主 题：Sodium citrate Lipid and protein deposition Insulin resistance Nile tilapia
摘 要：Citrate is an essential substrate for energy metabolism that plays critical roles in regulating glucose and lipid metabolic ***,the action of citrate in regulating nutrient metabolism in fish remains poorly ***,we investigated the effects of dietary sodium citrate on growth performance and systematic energy metabolism in juvenile Nile tilapia(Oreochromis niloticus).A total of 270Nile tilapia(2.81±0.01 g)were randomly divided into three groups(3 replicates per group,30 fish per replicate)and fed with control diet(35%protein and 6%lipid),2%and 4%sodium citrate diets,respectively,for 8 *** results showed that sodium citrate exhibited no effect on growth performance(P0.05).The whole-body crude protein,serum triglyceride and hepatic glycogen contents were significantly increased in the 4%sodium citrate group(P0.05).The 4%sodium citrate treatment significantly increased the serum glucose and insulin levels at the end of feeding trial and also in the glucose tolerance test(P0.05).The 4%sodium citrate significantly enhanced the hepatic phosphofructokinase activity and inhibited the expression of pyruvate dehydrogenase kinase isozyme 2 and phosphor-pyruvate dehydrogenase E1 component subunit alpha proteins(P0.05).Additionally,the 4%sodium citrate significantly increased hepatic triglyceride and acetyl-Co A levels,while the expressions of carnitine palmitoyl transferase 1a protein were significantly down-regulated by the 4%sodium citrate(P0.05).Besides,the 4%sodium citrate induced crude protein deposition in muscle by activating m TOR signaling and inhibiting AMPK signaling(P0.05).Furthermore,the 4%sodium citrate significantly suppressed serum aspartate aminotransferase and alanine aminotransferase activities,along with the lowered expression of pro-inflammatory genes,such as nfκb,tnfa and il8(P0.05).Although the 4%sodium citrate significantly increased phosphor-nuclear factor-k B p65 prote
维普期刊数据库
同方期刊数据库