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Exploring the molecular mechanism of Suoquan pill in the treatment of diabetic kidney disease based on network pharmacology,molecular docking,in vitro experiment

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Traditional Medicine Research 2024年第11期9卷 27-37页

作者： Zi-Jie Yan yu Kang Shu-Man Liu fang-yu wang Man Xiao Yi-Qiang Xie College of Traditional Chinese Medicine Hainan Medical UniversityHaikou 571199China Graduate School Heilongjiang Academy of Traditional Chinese MedicineHarbin 150001China Department of Pharmacology College of PharmacyHainan Medical UniversityHaikou 571199China Key Laboratory of Biochemistry and Molecular Biology Hainan Medical UniversityHaikou 571199China.

Background:Diabetic kidney disease(DKD)is a microvascular complication of diabetes mellitus and is the main cause of end-stage renal *** pills(SQP)has a variety of pharmacological activities and multiple therapeutic effects,and it is used clinically as a basic formula for the treatment of ***:Public databases were used to identify SQP compounds and the potential targets of SQP and DKD.A drug-component-therapeutic target network was ***-protein interaction network analysis,Gene Ontology functional analysis,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to analyse the potential molecular mechanisms of SQP based on common targets of drugs and *** docking simulations were conducted to confirm the binding abity of the core compounds to key *** efficacy and predicted molecular mechanisms of SQP were validated using cell counting kit-8 assay,flow cytometry,and western blotting with HK-2 cells as a ***:Network pharmacology analysis showed that 26 compounds and 207 potential targets of SQP were involved in the treatment of DKD;boldine,denudatin B,pinocembrin,kaempferoid,and quercetin were considered core compounds,and epidermal growth factor receptor(EGFR)and proto-oncogene,non-receptor tyrosine kinase(SRC)were considered key *** Ontology enrichment analysis indicated that protein phosphorylation and negative regulation of apoptotic processes are important biological processes in the treatment of DKD by *** docking confirmed the excellent binding abilities of boldine,denudatin B,kaempferide,and quercetin to EGFR and *** results of in vitro experiments showed that treatment with an ethanolic extract of SQP significantly protected HK-2 cells from high glucose-induced cell *** addition,the SQP ethanol extract inhibited the phosphorylation of EGFR and SRC,suppressed the apoptosis rate,and regulated apoptosis-related proteins in HK-2 cells under hig

关键词： traditional Chinese medicine diabetic kidney disease Suoquan pill network analysis molecular docking

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Whole exome sequencing identifies risk variants associated with intracranial epidermoid cyst deterioration:A case report

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World Journal of Clinical Oncology 2024年第11期15卷 1428-1434页

作者： Zhao-Na Song Yan Cheng Dan-Dan wang Ming-Jun Li Xiang-Rong Zhao Fa-wang Li Zhen Liu Xiao-Ru Zhu Xiao-Dong Jia yu-fang wang Feng-Fan Liang Joint Laboratory for Translational Medicine Research Liaocheng People's HospitalLiaocheng 252000Shandong ProvinceChina Harbin Genars Technology Co. Ltd.Harbin 150060Heilongjiang ProvinceChina Department of Radiotherapy Liaocheng People's HospitalLiaocheng 252000Shandong ProvinceChina Department of Medical laboratory Qilu Hospital of Shandong University Dezhou HospitalDezhou 253600Shandong ProvinceChina

BACKGROUND Intracranial epidermoid cyst(IEC)transformation to malignant squamous cell carcinoma(SCC)is extremely rare,and its etiology is yet ***,SCC is treated by performing surgery,followed by a combination of radiotherapy and *** is crucial to identify efficient and trustworthy therapeutic targets for SCC to improve its diagnosis,prognosis,and *** SUMMARY In this study,we report the case of a 47-year-old female patient with SCC,which progressed from IEC in the left internal capsule *** patient was sought treatment at our hospital for severe diplopic vision,accompanied with speech disorder and memory *** on the clinical and postoperative pathology,this patient was finally diagnosed with *** identify disease-causing variants,whole exome sequencing(WES)was performed on the *** revealed two pathogenic missense mutations on Gap junction protein beta 2(GJB2)(c.257C>T)and Toll-like receptor 2(TLR2)(c.1039A>G),*** This study provided the first clinical evidence for demonstrating the role of GJB2 and TLR2 in IEC development and *** further confirmed WES as a robust and reliable technique for underlying rare and complex disease-related genetic factor identification.

关键词： Intracranial epidermoid cyst Squamous cell carcinoma Whole exome sequencing Variants Case report

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Recent progress in microRNA study:Benefits from technique advance

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Science China(Life Sciences) 2012年第7期55卷 649-650页

作者： yu Jia wang fang Department of Biochemistry and Molecular Biology National Laboratory of Medical Molecular Biology Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100005 China

Over the last decade, sequencing of different genomes re- vealed that an increase in organismal complexity is not merely explained by a dramatic increase in the number of protein-coding genes. However, the gradual increase in protein diversity contributes a lot to the complexity of high- er organisms [1]. It is now widely accepted that non-coding RNAs （ncRNAs）, especially microRNAs （miRNAs） largely define eukaryotic cell functions and the impacts of a variety of gene regulation [2].

关键词：小分子RNA 技术进步蛋白编码基因非编码RNA 基因组测序 miRNA 细胞功能微RNA

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Magnetically assisted fluorescence ratiometric assays for adenosine deaminase using water-soluble conjugated polymers

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Chinese Science Bulletin 2009年第8期54卷 1340-1344页

作者： HE fang yu MingHui wang Shu Beijing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids institute of Chemistry Chinese Academy ofSciences Beijing 100190 China

A magnetically assisted fluorescence ratiometric technique has been developed for adenosine deaminase assays with high sensitivity using water-soluble cationic conjugated polymers(CCPs).The assay contains three elements:a biotin-labeled aptamer of adenosine(biotin-aptamer),a signaling probe single-stranded DNA-tagged fluorescein at terminus(ssDNA-Fl) and a *** specific binding of adenosine to biotin-aptamer makes biotin-aptamer and ssDNA-Fl unhybridized,and the ssDNA-Fl is washed out after streptavidin-coated magnetic beads are added and separated from the assay solution under magnetic *** this case,after the addition of CCP to the magnetic beads solution,the fluo-rescence resonance energy transfer(FRET) from CCP to fluorescein is *** adding adenosine deaminase,the adenosine is converted into inosine,and the biotin-aptamer is hybridized with ssDNA-Fl to form doubled stranded DNA(biotin-dsDNA-Fl).The ssDNA-Fl is attached to the mag-netic beads at the separation step,and the addition of CCP to the magnetic beads solution leads to efficient FRET from CCP to *** the adenosine deaminase activity can be monitored by fluorescence spectra in view of the intensity decrease of CCP emission or the increase of fluorescein emission in aqueous *** assay integrates surface-functionalized magnetic particles with significant amplification of detection signal of water-soluble cationic conjugated polymers.

关键词：腺苷脱氨酶共轭聚合物荧光素水溶性荧光共振能量转移单链DNA 生物素标记检测灵敏度

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Unfavorable outcome of antiviral therapy in cytomegaloviruspositive ulcerative colitis may be due to inappropriate study inclusion in meta-analysis

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World Journal of Gastroenterology 2015年第5期21卷 1689-1690页

作者： Xiao-Wei Wu Miao-fang Yang Nan Li fang-yu wang Department of Gastroenterology and Hepatology Jinling HospitalMedical School of Nanjing University

Some previous articles reported that antiviral treatment was effective to reduce the colectomy rate in ulcerative colitis(UC) patients with cytomegalovirus(CMV) *** et al recently carried out a systematic review and meta-analysis to evaluate the impact of antiviral therapy on CMV-positive *** results showed that patients who received antiviral treatmenthad a higher risk of 30-d *** found that in this meta-analysis,some studies were inappropriately included,leading to an unfavorable outcome of antiCMV therapy in UC patients.

关键词： Ulcerative colitis Cytomegalovirus Antiviral thera

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Grinding Properties of Abandoned Concrete

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Journal of China University of Mining and Technology 2007年第2期17卷 281-285页

作者： ZHENG fang-yu wang Li-jiu LI Qiang School of Civil and Hydraulic Engineering Dalian University of Technology Dalian Liaoning 116024 China

The grinding properties of abandoned concrete, which consists primarily of hardened cement, limestone aggregate and river sand, are studied. Theoretical models of grinding are used to explain the experimental observation. The results show that 1) The principle disintegration mechanism of hardened cement and river sand is volumetric grinding, although at later stages grinding of cement becomes difficult because of its flaked structure; 2) The lime- stone grinding process can be divided into two steps. First, volumetric grinding, with an obvious component of surface grinding, followed by primarily surface grinding as the micro-particle content increases; 3) Initially, the principle mechanism of grinding limestone and river sand is volumetric grinding, albeit less efficient grinding than if these components were ground separately, and; 4) After 10 to 20 min of grinding the grinding bottleneck phenomenon ap- pears and after 20 min of grinding the content of micro-particles is large and surface grinding is the main mechanism while the particle size of the mixture is smaller than that of separately ground river sand and cement but bigger than that of separately ground limestone.

关键词： abandoned concrete cement stone limestone river sand grinding

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Non-Noether Conserved Quantity of Poincaré-Chetaev Equations of a Generalized Classical Mechanics

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Communications in Theoretical Physics 2006年第6期45卷 961-964页

作者： ZHANG Peng-yu fang Jian-Hui wang Peng DING Ning College of Physics Science and Technology China University of Petroleum （East China） Dongying 257061 China

In the present paper the Lie symmetrical non-Noether conserved quantity of the Poincaré Chetaev equations of a generalized classical mechanics under the general infinitesimal transformations of Lie groups is discussed. First, we establish the determining equations of Lie symmetry of the equations. Second, the Lie symmetrical non-Noether conserved quantity of the equations is deduced. Finally, an example is given to illustrate the application of the results.

关键词： Poincaré-Chetaev equations generalized classical mechanics Lie symmetry non-Noether conserved quantity

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Conformations and adsorption behavior of poly(allylamine hydrochloride) studied by single molecule force spectroscopy

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Chinese Science Bulletin 2008年第1期53卷 22-26页

作者： yu Ying wang fang SHI WeiQing wang LiYan wang WenBin SHEN JiaCong Key Lab of Supramolecular Structure and Materials Jilin University Changchun 130012 China

Poly(allylamine hydrochloride) (PAH), which is frequently used in fabricating polyelectrolyte multilayer films, was studied by single molecule force spectroscopy (SMFS). Plenty of force-extension curves with a long plateau were obtained in water, indicating that train-like structure was predominant when PAH was adsorbed on the substrate. It was found that the peak-type force-extension curves of PAH in water were not able to be fitted by the modified freely-jointed chain model. Additionally, there was a flat region in the derivative of force-extension curves. Thus, it was inferred that PAH chain in water was in a special conformation and underwent a "conformational transition" under the stretching of an external force. This phenomenon did not appear in the SMFS experiment in 1 mol/L urea solution, which indi-cated that urea was able to break the special conformation.

关键词：光谱学单分子吸附丙烯胺

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Gastrocolic fistula in Crohn’s disease detected by oral agent contrast-enhanced ultrasound: A case report of a novel ultrasound modality

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World Journal of Gastroenterology 2020年第17期26卷 2119-2125页

作者： Shuang Wu Hua Zhuang Jie-Ying Zhao yu-fang wang Department of Diagnostic Ultrasound West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Gastroenterology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND Fistulas are common complications of Crohn’s disease(CD).Gastrocolic fistulas(GFs)are rare,occult and potentially life-threatening *** cases of GFs have been *** agent contrast-enhanced ultrasound(OA-CEUS)is a novel technique of ultrasound(US)for *** agent made by Chinese yam is taken orally to dilate the lumen of the upper gastrointestinal ***,the impediment of gas inside gastrointestinal tract is removed and a good acoustic window is provided for gastroin-testinal tract *** paper describes a case of GF secondary to CD detected by OA-CEUS when it was missed by endoscopy and computed tomography(CT).To our knowledge,this is the first report of GF secondary to CD detected by OA-CEUS up to *** SUMMARY A 29-year-old woman with a 6-year history of CD was admitted to our hospital for abdominal pain and diarrhea for 5 months without obvious predisposing *** gastroscopy failed to show any evidence of *** revealed multiple erosions,mucosal nodularity,linear ulcers and a cobblestone appearance.A CT scan of her abdomen showed a complex multilocular structure adherent to the greater curvature of the stomach in her left lower abdomen,with fluid,gas and significant surrounding *** also demonstrated an abdominal abscess,which was later treated with US-guided ***,gastroscopy and CT missed the presence of a ***-CEUS was performed.A contrast agent made from Chinese yam was taken orally to dilate the lumen of the gastrointestinal tract.A good acoustic window was provided for gastrointestinal tract scanning and the impediment of gas inside the gastrointestinal tract was *** the aid of the“window”,a canal with hypoechoic wall was identified connecting the greater curvature of stomach to the splenic colon flexure in free *** also observed the hyperechoic gas flowing dynamically inside the ***,a GF was *** is the first imaging modali

关键词： Gastrocolic fistula Ultrasound Oral agent Crohn’s disease Complication Case report

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Extracellular ATP Promotes Stomatal Opening of Arabidopsis thaliana through Heterotrimeric G Protein α Subunit and Reactive Oxygen Species

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Molecular Plant 2012年第4期5卷 852-864页

作者： Li-Hua Hao Wei-Xia wang Chen Chen yu-fang wang Ting Liu Xia Li Zhong-Lin Shang a Key Laboratory of Molecular and Cell Biology of Hebei Province College of Life SciencesHebei Normal UniversityShijiazhuang 050016RR.China State Key Laboratory of Plant Cell and Chromosome Engineering Center of Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021P.R.China

In recent years, adenosine tri-phosphate （ATP） has been reported to exist in apoplasts of plant cells as a signal molecule. Extracellular ATP （eATP） plays important roles in plant growth, development, and stress tolerance. Here, extra- cellular ATP was found to promote stomatal opening of Arabidopsis thaliana in light and darkness. ADP, GTP, and weakly hydrolyzable ATP analogs （ATPγS, Bz-ATP, and 2meATP） showed similar effects, whereas AMP and adenosine did not affect stomatal movement. Apyrase inhibited stomatal opening. ATP-promoted stomatal opening was blocked by an NADPH oxidase inhibitor （diphenylene iodonium） or deoxidizer （dithiothreitol）, and was impaired in null mutant of NADPH ox- idase （atrbohD/F）. Added ATP triggered ROS generation in guard cells via NADPH oxidase. ATP also induced Ca^2＋ influx and H ＋ efflux in guard cells. In atrbohD/F, ATP-induced ion flux was strongly suppressed. In null mutants of the heterotrimeric G protein α subunit, ATP-promoted stomatal opening, cytoplasmic ROS generation, Ca^2＋ influx, and ^H＋ efflux were all sup- pressed. These results indicated that eATP-promoted stomatal opening possibly involves the heterotrimeric G protein, ROS, cytosolic Ca^2＋, and plasma membrane H＋-ATPase.

关键词： Extracellular ATP stomatal movement Arabidopsis thaliana heterotrimeric G protein reactive oxygenspecies.

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