Exploring the molecular mechanism of Suoquan pill in the treatment of diabetic kidney disease based on network pharmacology,molecular docking,in vitro experiment
作者机构：College of Traditional Chinese MedicineHainan Medical UniversityHaikou 571199China Graduate SchoolHeilongjiang Academy of Traditional Chinese MedicineHarbin 150001China Department of PharmacologyCollege of PharmacyHainan Medical UniversityHaikou 571199China Key Laboratory of Biochemistry and Molecular BiologyHainan Medical UniversityHaikou 571199China.
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2024年第9卷第11期
页 面：27-37页
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：supported by the grants from National Natural Science Foundation of China(No.82174334) Hainan Province in 2022 postgraduate innovation research projects(No.Qhys2022-273)
主 题：traditional Chinese medicine diabetic kidney disease Suoquan pill network analysis molecular docking
摘 要：Background:Diabetic kidney disease(DKD)is a microvascular complication of diabetes mellitus and is the main cause of end-stage renal *** pills(SQP)has a variety of pharmacological activities and multiple therapeutic effects,and it is used clinically as a basic formula for the treatment of ***:Public databases were used to identify SQP compounds and the potential targets of SQP and DKD.A drug-component-therapeutic target network was ***-protein interaction network analysis,Gene Ontology functional analysis,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to analyse the potential molecular mechanisms of SQP based on common targets of drugs and *** docking simulations were conducted to confirm the binding abity of the core compounds to key *** efficacy and predicted molecular mechanisms of SQP were validated using cell counting kit-8 assay,flow cytometry,and western blotting with HK-2 cells as a ***:Network pharmacology analysis showed that 26 compounds and 207 potential targets of SQP were involved in the treatment of DKD;boldine,denudatin B,pinocembrin,kaempferoid,and quercetin were considered core compounds,and epidermal growth factor receptor(EGFR)and proto-oncogene,non-receptor tyrosine kinase(SRC)were considered key *** Ontology enrichment analysis indicated that protein phosphorylation and negative regulation of apoptotic processes are important biological processes in the treatment of DKD by *** docking confirmed the excellent binding abilities of boldine,denudatin B,kaempferide,and quercetin to EGFR and *** results of in vitro experiments showed that treatment with an ethanolic extract of SQP significantly protected HK-2 cells from high glucose-induced cell *** addition,the SQP ethanol extract inhibited the phosphorylation of EGFR and SRC,suppressed the apoptosis rate,and regulated apoptosis-related proteins in HK-2 cells under hig
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