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Characterization of Fam20C expression in odontogenesis and osteogenesis using transgenic mice

International Journal of Oral Science 2015年第2期7卷 89-94页

作者： Er-Xia Du Xiao-Fang Wang wu-Chen Yang Deborah Kaback Siu-Pok Yee Chun-Lin Qin Anne George jian-Jun Hao Division of Orthodontics Department of Craniofacial SciencesSchool of Dental MedicineUniversity of Connecticut Department of Biomedical Sciences Texas A&M Health Science CenterBaylor College of Dentistry Department of Genetics and Developmental Biology University of Connecticut Health Center Department of Oral Biology University of Illinois

Our previous studies have demonstrated that Fam20 C promotes differentiation and mineralization of odontoblasts,ameloblasts,osteoblasts and osteocytes during tooth and bone *** of the Fam20 C gene inhibits bone and tooth growth by increasing fibroblast growth factor 23 in serum and causing hypophosphatemia in conditional knockout ***,control and regulation of the expression of Fam20 C are still *** this study,we generated a transgenic reporter model which expresses green fluorescence protein（GFP） driven by the Fam20 C *** was used to insert a 16 kb fragment of the mouse Fam20 C gene（containing the 15 kb promoter and 1.1 kb of exon 1） intoa pBluescript SK vector with the topaz variant of GFP and a bovine growth hormone polyadenylation *** expression was subsequently evaluated by histomorphometry on cryosections from E14 to adult *** was evident in the bone and teeth as early as *** GFP signal was maintained stably in odontoblasts and osteoblasts until 4 weeks after *** expression of GFP was significantly reduced in teeth,alveolar bone and muscle by 8 weeks of *** also observed colocalization of the GFP signal with the Fam20 C antibody in postnatal 1- and 7-day-old *** generation of Fam20C-GFP transgenic mice will provide a unique model for studying Fam20 C gene expression and the biological function of this gene during odontogenesis and osteogenesis.

关键词： transgenic promoter pBluescript osteogenesis teeth transgene reporter Fluorescence insert alveolar

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Isolation and Culture of a kind of Derived Cells from Differentiation Group Of Murine ESC-like Cell and Their Traits in Culture

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生物医学工程学杂志 2005年第S1期22卷 177-178页

作者： Qi-Shuang GAO Ting yu Bang-You LI Yan-yun HAN Chun-Han AIChun-Fang WANG Jing-Fu LV jian-Ying wu(wuhan Institute of Pasturage and Veterinary,wuhan 430000,China) wuhan Institute of Pasturage and Veterinary Wuhan 430000 China

1 IntroductionIn the process of culturing ESC-like cell from murine blastula,we find that even if no inducing factor was added, ESC-like cell masses can different into many kinds of cell groups. Study on the biological traits, culturing and change of functional genetics of those cell groups will be of great meaning to find out the mechanism of differentiation and possible value of ESC-like cells. UP to now, there are not many references and reports on this aspect. This article introduces a kind of derived cells isolated from ESC-like cell, the change of its growth, and need of culturing conditions, which may be useful in further study on ESC-like cells.

关键词： ESC Isolation and Culture of a kind of Derived Cells from Differentiation Group Of Murine ESC-like Cell and Their Traits in Culture

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永磁同步电机电流扰动补偿无位置传感器控制

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电机与控制学报 2020年第4期24卷 80-86,95页

作者：吴建华吴航浙江大学电气工程学院杭州310000

电机负载扰动及参数变化等问题,会影响永磁同步电机无位置传感器控制中滑模观测器算法的电流计算值偏离实际值,进而影响转速和位置的准确估算。本文以小功率面贴式永磁同步电机为研究对象,提出一种采用电流扰动补偿的新型滑模观测器(CDC... 详细信息

电机负载扰动及参数变化等问题,会影响永磁同步电机无位置传感器控制中滑模观测器算法的电流计算值偏离实际值,进而影响转速和位置的准确估算。本文以小功率面贴式永磁同步电机为研究对象,提出一种采用电流扰动补偿的新型滑模观测器(CDC-SMO),通过构建带有扰动补偿电流与实际电流的误差状态方程,将扰动量和电流的微分项进行合并,构成等效扰动量以直接获得电流的误差,并用反正切函数替代理想滑动模态中的符号函数,实现带有电流扰动补偿的永磁同步电机无位置传感器控制,最后,利用李雅普诺夫稳定性判据证明了观测器的稳定性。仿真和实验表明,所提方法可以准确地估算永磁同步电机的转速和位置,有效提高无位置传感器控制的估算精度和抗干扰能力。

关键词：永磁同步电机无位置传感器控制滑模观测器电流扰动补偿等效扰动量抗干扰能力

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The Effect of Laser Acupuncture on the Levels of IL-2,IL-6 and TGF-β1in Serum of Rats with Atrophic Gastritis

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Chinese Journal of Biomedical Engineering(English Edition) 2015年第4期24卷 160-165页

作者： SHAO Xue-hui WANG jian-guo MA Yi-fei LI Dan-dan GAO jian-wu YANG yue-pin Hebei North University

Objective： To study the effect of three different methods such as medicine, acupuncture and laser acupuncture on the levels of interleukin 2 （IL-2）, inter- leukin 6 （IL-6） and transforming growth factor-β1 （TGF-β1） in rat＇s serum with chronic atrophic gastritis. Methods： 9 rats were randomly selected from 60 rats as normal con- trol group, and the other rats were used to replicate the animal model of chronic atrophic gastritis with comprehensive method, 5 rats were randomly inspected at the 8th week in the process of creating model for gastric mucosa pathological examination; when gastric mucosa shows CAG symptoms such as various degree of congestion, bleeding, atrophy, metaplasia, the model rats were randomly divided into model group, medicine group, acupuncture group and laser acupuncture group. The rats of medicine group were chron- ically administered with yanshenjianwei capsule and Kangfuxin Liquid; the groups of acupuncture and laser acupuncture selected Zusanli acupuncture as acupuncture points, after the treatment for 14 d, blood was removed, double antibody sandwich en- zyme linked immunosorbent assay （ELISA） was used to detect the levels of IL-2, IL-6 and TGF-β1 in serum. Results： Compared with normal group, the contents of IL-2, IL-6 and TGF-β1 in serum of model rats increased （P〈0.05 or P〈0.01）. Medicine, acupuncture and laser acupuncture could reduce the contents of IL-2, IL-6 and TGF-β1, IL-2 in serum of the medicine group and laser acupuncture group was signifi- cantly decreased （P〈0.05）; IL-6 in acupuncture group and laser acupuncture group were significantly decreased （P〈0.05 or P〈0.01）;and the most significant is laser acupuncture group （P〈0.01）. Medicine, acupuncture and laser acupuncture could significantly reduce TGF-β 1 content in serum （P〈0.05 or P〈0.01）, and the particularly significant was laser acupuncture（P〈0.01）. Conclusion： Laser acupuncture has the function of reducing the inflammatory response and adjusting immune function. It earl eff

关键词： chronic atrophic gastritis (CAG) laser acupuncture interleukin-2 (IL-2) interleukin-6 (IL-6) transforming growth factor-J51 (TGF-β1)

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Neuroprotective effects of long noncoding RNAs involved in ischemic postconditioning after ischemic stroke

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Neural Regeneration Research 2022年第6期17卷 1299-1309页

作者： Wei Ma Chun-Yan Li Si-Jia Zhang Cheng-Hao Zang Jin-Wei Yang Zhen wu Guo-Dong Wang Jie Liu Wei Liu Kuang-Pin Liu yu Liang Xing-Kui Zhang Jun-Jun Li jian-Hui Guo Li-Yan Li Institute of Neuroscience Kunming Medical UniversityKunmingYunnan ProvinceChina Second Department of General Surgery First People’s Hospital of Yunnan ProvinceKunmingYunnan ProvinceChina

During acute reperfusion,the expression profiles of long noncoding RNAs in adult rats with focal cerebral ischemia undergo broad ***,whether long noncoding RNAs are involved in neuroprotective effects following focal ischemic stroke in rats remains *** this study,RNA isolation and library preparation was performed for long noncoding RNA sequencing,followed by determining the coding potential of identified long noncoding RNAs and target gene *** expression analysis,long noncoding RNA functional enrichment analysis,and co-expression network analysis were performed comparing ischemic rats with and without ischemic postconditioning *** were subjected to ischemic postconditioning via the brief and repeated occlusion of the middle cerebral artery or femoral *** real-time reverse transcription-polymerase chain reaction was used to detect the expression levels of differentially expressed long noncoding RNAs after ischemic postconditioning in a rat model of ischemic *** results showed that ischemic postconditioning greatly affected the expression profile of long noncoding RNAs and mRNAs in the brains of rats that underwent ischemic *** predicted target genes of some of the identified long noncoding RNAs(cis targets)were related to the cellular response to ischemia and stress,cytokine signal transduction,inflammation,and apoptosis signal transduction *** addition,15 significantly differentially expressed long noncoding RNAs were identified in the brains of rats subjected to ischemic *** candidate long noncoding RNAs that may be related to ischemic postconditioning were identified by a long noncoding RNA expression profile and long noncoding RNA-mRNA co-expression network *** levels were verified by quantitative real-time reverse transcription-polymerase chain *** results suggested that the identified long noncoding RNAs may be involved in the neuropr

关键词： cerebral infarction differential expression analysis expression profiling GO term ischemic postconditioning ischemic stroke KEGG pathway lncRNA mRNA RNA sequencing

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SYK-mediated epithelial cell state is associated with response to c-Met inhibitors in c-Met-overexpressing lung cancer

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Signal Transduction and Targeted Therapy 2023年第6期8卷 3098-3110页

作者： Ji Zhou Xu-Chao Zhang Shan Xue Mengdi Dai yueliang Wang Xia Peng jianjiao Chen Xinyi Wang Yanyan Shen Hui Qin Bi Chen yu Zheng Xiwen Gao Zuoquan Xie jian Ding Handong jiang Yi-Long wu Meiyu Geng Jing Ai Division of Antitumor Pharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China University of Chinese Academy of Sciences No.19A Yuquan RoadBeijing100049China Guangdong Provincial Key Laboratory of Translational Medicine in Lung Cancer Guangdong Lung Cancer InstituteGuangdong Provincial People’s Hospitaland Guangdong Academy of Medical SciencesSchool of MedicineSouth China University of TechnologyGuangzhou510080China Department of Respiratory Medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai200127China Department of Neurobiology Brain InstituteUniversity of PittsburghPittsburgh15213USA Department of Respiratory Medicine Affiliated Hospital of Xuzhou Medical UniversityXuzhou221000China Department of Respiratory Medicine Minhang HospitalFudan UniversityShanghai201199China School of Pharmaceutical Science and Technology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou310024P.R.China

Genomic MET amplification and exon 14 skipping are currently clinically recognized biomarkers for stratifying subsets of non-small cell lung cancer(NSCLC)patients according to the predicted response to c-Met inhibitors(c-Metis),yet the overall clinical benefit of this strategy is quite ***,c-Met protein overexpression,which occurs in approximately 20–25%of NSCLC patients,has not yet been clearly defined as a clinically useful *** optimized strategy for accurately classifying patients with c-Met overexpression for decision-making regarding c-Meti treatment is ***,we found that SYK regulates the plasticity of cells in an epithelial state and is associated with their sensitivity to c-Metis both in vitro and in vivo in PDX models with c-Met overexpression regardless of MET gene ***,TGF-β1 treatment resulted in SYK transcriptional downregulation,increased Sp1-mediated transcription of FRA1,and restored the mesenchymal state,which conferred resistance to ***,a subpopulation of NSCLC patients with c-Met overexpression coupled with SYK overexpression exhibited a high response rate of 73.3%and longer progression-free survival with c-Meti treatment than other *** negativity coupled with TGF-β1 positivity conferred de novo and acquired *** summary,SYK regulates cell plasticity toward a therapy-sensitive epithelial cell ***,our findings showed that SYK overexpression can aid in precisely stratifying NSCLC patients with c-Met overexpression regardless of MET alterations and expand the population predicted to benefit from c-Met-targeted therapy.

关键词： SYK alterations state

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碳分子线C_5在激光场中的含时密度泛函理论研究

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物理学报 2013年第12期62卷 217-222页

作者：王志萍陈健吴寿煜吴亚敏江南大学理学院无锡214122

运用含时密度泛函理论和分子动力学相结合的方法,研究了C5分子线在强激光场中的电离激发.研究发现,当考虑激光强度对C5分子线激发的影响时,激光强度越强,分子吸收的能量越多,电离也越早,最终电离的电子也越多,而且沿激光极化方向的偶极... 详细信息

运用含时密度泛函理论和分子动力学相结合的方法,研究了C5分子线在强激光场中的电离激发.研究发现,当考虑激光强度对C5分子线激发的影响时,激光强度越强,分子吸收的能量越多,电离也越早,最终电离的电子也越多,而且沿激光极化方向的偶极矩的变化及峰值也越大.关于激光极化方向对C5分子线激发的影响的研究表明,当激光极化方向沿着C5分子线轴向时,分子的电离大大增强,x方向的激光脉冲仅能激发起x方向的偶极振荡,而y方向的激光脉冲仅能激发起y方向的偶极振荡,而且x方向的激光脉冲激发的偶极振荡强.研究还表明,当激光极化方向沿着C5分子线轴向时,尽管由于电离增强而导致C5分子线C—C键振动的同步性变差,但在两种激光极化方向情况下,C5分子线的振动模式与中性C5分子线的振动模式相同.

关键词：含时密度泛函理论分子动力学分子电离碳分子线

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Temperature dependence of mode coupling effect in piezoelectric vibrator made of[001]c-poled Mn-doped 0.24PIN-0.46PMN-0.30PT ternary single crystals with high electromechanical coupling factor

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Chinese Physics B 2020年第7期29卷 363-368页

作者： Nai-Xing Huang En-Wei Sun Rui Zhang Bin Yang jian Liu Tian-Quan Lv Wen-wu Cao Department of Physics Northeast Petroleum UniversityDaqing 163318China The School of Instrumentation Science and Engineering Harbin Institute of TechnologyHarbin 150001China Department of Mathematics and Materials Research Institute The Pennsylvania State UniversityUniversity ParkPA 16802USA

The influence of temperature on mode coupling effect in piezoelectric vibrators remains *** this work,we discuss the influence of temperature on two-dimensional(2D)mode coupling effect and electromechanical coupling coefficient of cylindrical[001]c-poled Mn-doped 0.24PIN-0.46PMN-0.30PT piezoelectric single-crystal vibrator with an arbitrary configuration *** electromechanical coupling coefficient kt decreases with temperature increasing,whereas k33 is largely invariant in a temperature range of 25℃-55℃.With the increase of temperature,the shift in the‘mode dividing point’increases the scale of the poling direction of the piezoelectric *** temperature has little effect on coupling constantΓ.At a given temperature,the coupling constantΓof the cylindrical vibrator is slightly greater than that of the rectangular *** the temperature changes,the applicability index(M)values of the two piezoelectric vibrators are close to 1,indicating that the coupling theory can be applied to piezoelectric vibrators made of late-model piezoelectric single crystals.

关键词： mode coupling effect piezoelectric vibrator piezoelectric single crystal temperature dependence

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In Situ Loading and Delivery of Short Single- and Double-Stranded DNA by Supramolecular Organic Frameworks

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CCS Chemistry 2019年第2期1卷 156-165页

作者： Bo Yang Xiao-Dan Zhang jian Li Jia Tian Yi-Peng wu Fa-Xing yu Ruibing Wang Hui Wang Dan-Wei Zhang Yi Liu Lu Zhou Zhan-Ting Li Department of Chemistry Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and Collaborative Innovation Center of Chemistry for Energy Materials(iChEM)Fudan UniversityShanghai 200438China Department of Medicinal Chemistry School of PharmacyFudan UniversityShanghai 201203China Children’s Hospital and Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipaMacaoChina The Molecular Foundry Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA

Short DNA represents an important class of bioma-cromolecules that arewidely applied in gene therapy,editing,and ***,the development of simple and reliable methods for their intracellular delivery remains a ***,we describe that seven water-soluble,homogeneous supramole-cular organic frameworks(SOFs)with a well-defined pore size and high stability in water that can accom-plish in situ inclusion of single-stranded(ss)and double-stranded(ds)DNA(21,23,and 58 nt)and effective intracellular delivery(including two non-cancerous and six cancerous cell lines).

关键词： nucleic acid delivery homogeneous car-rier electrostatic interaction self-assembly porous polymer

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Numerical simulation of blood flow and interstitial fluid pressure in solid tumor microcirculation based on tumor-induced angiogenesis

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Acta Mechanica Sinica 2007年第5期23卷 477-483页

作者： Gaiping Zhao Jie wu Shixiong Xu M. W. Collins Quan Long Carola S. Konig yuping jiang jian Wang A. R. Padhani Department of Mechanics and Engineering Science Fudan University Shanghai 200433 China Brunel Institute for Bioengineering School of Engineering and Design Brunel University Uxbridge Middlesex UK Department of Neurology Huashan Hospital Fudan University Shanghai 200040 China Paul Strickland Scanner Centre Mount Vernon Hospital Rickmansworth Road Northwood Middlesex HA6 2RN UK

A coupled intravascular-transvascular-interstitial fluid flow model is developed to study the distributions of blood flow and interstitial fluid pressure in solid tumor microcirculation based on a tumor-induced microvascular network. This is generated from a 2D nine-point discrete mathematical model of tumor angiogenesis and contains two parent vessels. Blood flow through the microvascular network and interstitial fluid flow in tumor tissues are performed by the extended Poiseuille＇s law and Darcy＇s law, respectively, transvascular flow is described by Starling＇s law; effects of the vascular permeability and the interstitial hydraulic conductivity are also considered. The simulation results predict the heterogeneous blood supply, interstitial hypertension and low convection on the inside of the tumor, which are consistent with physiological observed facts. These results may provide beneficial information for anti-angiogenesis treatment of tumor and further clinical research.

关键词： Solid tumor pressure-Angiogenesis Blood flow - Interstitial Numerical simulation

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