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Science China(Life Sciences) 2009年第3期52卷 205-211页

作者： SHI Yi & JIN youxin State key laboratory of molecular biology,Shanghai Institute of Biochemistry and Cell biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China State key laboratory of molecular biology Shanghai Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The regulation of gene expression by microRNAs(miRNAs) is a recently discovered pattern of gene regulation in animals and *** have been implicated in various aspects of animal development and cell differentiation,such as early embryonic development,neuronal development,muscle development,and lymphocyte development,by the analysis of genetic deletions of individual miRNAs in *** studies show that miRNAs are key regulators in animal development and are potential causes of human *** we review some recent discoveries about the functions of miRNAs in cell differentiation and development.

关键词： miRNA embryonic development neuronal development muscle development lymphocyte development

Metal-amyloid-β peptide interactions: a preliminary investigation of molecular mechanisms for Alzheimer’s disease

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Science China Chemistry 2007年第4期50卷 453-467页

作者： JIAO Yong & YANG Pin key laboratory of Chemical biology and molecular Engineering of Ministry of Education, Institute of molecular Science, Shanxi University, Taiyuan 030006, China 1. key laboratory of Chemical biology and molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Although humans have spent exactly 100 years combating Alzheimer’s disease (AD), the molecular mechanisms of AD remain unclear. Owing to the rapid growth of the oldest age groups of the popula-tion and the continuous increase of the incidence of AD, it has become one of the crucial problems to modern sciences. It would be impossible to prevent or reverse AD at the root without elucidating its molecular mechanisms. From the point of view of metal-amyloid-β peptide (Aβ) interactions, we review the molecular mechanisms of AD, mainly including Cu2+ and Zn2+ inducing the aggregation of Aβ, cata-lysing the production of active oxygen species from Aβ, as well as interacting with the ion-channel-like structures of Aβ. Moreover, the development of therapeutic drugs on the basis of metal-Aβ interactions is also briefly introduced. With the increasingly rapid progress of the molecular mechanisms of AD, we are now entering a new dawn that promises the delivery of revolutionary developments for the control of dementias.

关键词： Alzheimer’s disease (AD) amyloid-β peptide Cu2+ Zn2+ molecular mechanisms aggregation active oxygen species ion channel

Effects of La^(3+) and Eu^(3+) on outward potassium channels in Vicia guard cells

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Science China Chemistry 2009年第11期52卷 1821-1824页

作者： XUE ShaoWu & YANG Pin key laboratory of Chemical biology and molecular Engineering of Ministry of Education,Institute of molecular Science,Shanxi University,Taiyuan 030006,China 1. key laboratory of Chemical biology and molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan 030006 China

Effects of La3+ and Eu3+ on outward potassium channels(K+out) in Vicia guard cells have been studied by patch clamping *** La3+ inhibited K+out currents with a half-inhibition concentration(IC50) of 81 μmol·***,intracellular La3+ activated K+out currents at a free concentration of 1.13 × 10-14 mol·L-1,and inhibited K+out currents at a free concentration of 5.86 × 10-14 mol·*** Eu3+ also activated K+out currents at concentrations of 10 μmol·L-1 and 50 μmol·L-1,and inhibited K+out currents at concentrations of more than 1 mmol·*** effects of La3+ and Eu3+ on K+out currents may contribute to regulation of the plant water status,which may be one of the mechanisms of the biological effect of rare earth elements.

关键词： rare earth elements stomata guard cell outward potassium channel

Cleavage of Supercoiled Circular Double-stranded DNA Induced by a Eukaryotic Cambialistic Superoxide Dismutase from Cinnamomum camphora

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 609-617页

作者： Bao-Zhong WANG, Xu-Bin WEI&, and Wang-Yi LIU* State key laboratory of molecular biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200031, China State key laboratory of molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

A eukaryotic cambialistic superoxide dismutase (SOD) has been purified to homogeneity from mature seeds of the disease- and insect-resistant camphor tree (Cinnamomum camphora). Besides the known role of this SOD in protecting cells against oxidative stress, it can induce the cleavage of supercoiled double-stranded DNA into nicked and linear DNA. It can not cleave linear DNA or RNA, demonstrating there is no DNase or RNase in the purified cambialistic SOD. Furthermore, the SOD can linearize circular pGEM-4Z DNA that is relaxed by topoisomerase I. This result indicates that the DNA-cleaving activity re- quires substrates being topologically constrained. The supercoiled DNA-cleaving activity of the cambialistic SOD can be inhibited by either SOD inhibitor (azide) or catalase and hydroxyl radical scavengers (ethanol and mannitol). The chelator of iron, diethylenetriaminepentaacetic acid (DTPA), also inhibits the supercoiled DNA-cleaving activity. These results show that the dismutation activity is crucial for the supercoiled DNA cleavage. The modification of tryptophan residue of the cambialistic SOD with N-bromosuccinimide (NBS) shows that these two activities are structurally correlative. The reaction mechanism is proposed that the hydroxyl radical formed in a transition-metal-catalyzing Fenton-type reaction contributes to the DNA- cleaving activity. In addition, the cleavage sites in supercoiled pGEM-4Z DNA are random.

关键词： cambialistic SOD Fenton-type reaction hydroxyl radical relaxed circular duplex DNA supercoiled DNA cleavage

Regulation of mammalian pre-mRNA splicing

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Science China(Life Sciences) 2009年第3期52卷 253-260页

作者： HUI JingYi State key laboratory of molecular biology,Institute of Biochemistry and Cell biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 1. State key laboratory of molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

In eukaryotes,most protein-coding genes contain introns which are removed by precursor messenger RNA(pre-mRNA) *** splicing is a process by which multiple messenger RNAs(mRNAs) are generated from a single pre-mRNA,resulting in functionally distinct *** genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene ***-regulation of splicing causes a wide range of human *** review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA *** also discusses emerging directions in the field of alternative splicing.

关键词： pre-mRNA splicing alternative splicing splicing regulation signal transduction microRNA

Cinnamomin——a Versatile Type Ⅱ Ribosome-inactivating Protein

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 169-176页

作者： Hong XU and Wang-Yi LIU State key laboratory of molecular biology, the Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China State key laboratory of molecular biology the Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Ribosome-inactivating proteins (RIPs) are a group of toxic proteins that can specifically act on the universally conserved sarcin/ricin domain (S/R domain) of the largest RNA in ribosome and thus irreversibly inactivate ribosome for protein synthesis. Cinnamomin is a multifunctional type II RIP isolated in our laboratory from the mature seeds of the camphor tree. This protein has been extensively studied with regard to its purification, characteristics, structure and function, genetic expression, enzymatic mechanism, physiological role in seed cell and toxicity to cancer cells and insect larvae. The research results of cinnamomin obtained in our laboratory are summarized in this review. Understanding of cinnamomin and the relative new proteins will help expand our knowledge of RIPs and may accelerate theoretical study and the development of their potential applications.

关键词： cinnamomin cinphorin ribosome-inactivating protein

Characterization and Crystal Structure of a New Holmium(III) Complex with the Macrocyclic Ligand Derived from 2,6-Diformyl-4-methylphenol and Diethylenetriamine

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Chinese Journal of Structural Chemistry 2007年第12期26卷 1429-1434页

作者：胡学雷邱立赵元弟key laboratory of Biomedical Photonics of Ministry of Education - Wuhan National laboratory for Optoelectronics - Hubei Bioinformatics and molecular Imaging key laboratory - key laboratory of molecular Biophysics of the Ministry of Education Huazhong University of Science and Technology 潘志权 key laboratory of Biomedical Photonics of Ministry of Education - Wuhan National laboratory for Optoelectronics - Hubei Bioinformatics and molecular Imaging key laboratory - key laboratory of molecular Biophysics of the Ministry of Education Huazhong University of Science and Technology Hubei key laboratory of Novel Chemical Reactor and Green Chemical Technology Wuhan Institute of Technology

The title compound （13,27-dimethyl-3,6,9,17,20,23-hexaazatricyclo-[23.***.1.1^11.15]- triaconta-l（29）,2,9,11,13,15（30）,16,23,25,27-decaene-29,30-diol-N^3,N^6,N^9,O^29,O^30）-bis（nitrato-O,O＇）- holmium（m） nitrate hydrate has been prepared and characterized by elemental analysis, infrared spectra, and electrospray mass spectra. Its crystal and molecular structures were determined by X-ray diffraction methods. The crystal crystallizes in the monoclinic system, space group C2/c with a = 23.737（12）, b = 14.237（7）, c = 19.801（10） A, β = 91.36（1）^o, Mr = 831.57, V = 6690（6） A^3, Z = 8, Dc = 1.651 g/cm^3, F（000） = 3344, R = 0.0482 and wR = 0.0923. The holmium ion is located in one of the compartments of the macrocyclic ligand and presents a distorted tricapped trigonal prismatic coordination geometry. The macrocycle is coordinated with two oxygen and three nitrogen atoms. Two nitrates are chelated in the opposite positions of the macrocycle, and the third one is ionic.

关键词： crystal structure lanthanide macrocycle

An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 571-576页

作者： Li-Qing XIAO, Ai-Hua LIU, and Yong-Lian ZHANG State key laboratory of molecular biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China State key laboratory of molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family.

关键词： Bin1b epididymis β-defensin double-copy protein polyclonal antiserum

Iron metabolism in the mononuclear phagocyte system

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Progress in Natural Science:Materials International 2008年第10期18卷 1197-1202页

作者： Weina Kong Xianglin Duan Zhenhua Shi Yanzhong Chang The key laboratory of Animal Physiology,Biochemistry and molecular biology of Hebei Province,Hebei Normal University,Shijiazhuang 050016,China The key laboratory of Animal Physiology Biochemistry and Molecular Biology of Hebei Province Hebei Normal University Shijiazhuang 050016 China

The maintenance of body iron homeostasis requires the coordination of multiple regulatory mechanisms of iron *** mononuclear phagocyte system (MPS,composed of monocytes,macrophages,and their precursor cells) is crucial in the maintenance of iron *** of iron is carried out by specialized macrophages via engulfment of aged *** iron stores of macrophages depend on the levels of recovered and exported ***,the molecular mechanisms underlying iron homeostasis in macrophages are poorly *** studies characterizing the function and regulation of natural resistance-associated mac- rophage protein I (Nrampl),divalent metal transporter 1 (DMTI),HLA-linked hemechromatosis gene (HFE),ferroportin 1 (FPNI), and hepcidin are rapidly expanding our knowledge on the molecular level of MPS iron *** studies are deepening our under- standing about the molecular mechanism of iron homeostasis and iron-related diseases.

关键词： Mononuclear phagocyte system CD 163 Natural resistance-associated macrophage protein 1 Divalent metal transporter 1 HFE Ferroportin 1 Hepcidin