A novel snoRNA can direct site-specific 2’-O-ribose methylation of snRNAs in Oryza Sativa
会议名称：《第三届全国暨2003年中外RNA研讨会》
会议日期：2003年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
关 键 词：2’-O-ribose methylation box C/D snoRNA snRNA oryza sativa plant
摘 要：正 Small nucleolar RNAs （snoRNA） are a kind of noncoding RNAs, and the vast majority of snoRNAs are involved in site-specific modifications of rRNAs. A novel box C/D snoRNA called snoR124 was found in oryza sativa, and it can direct 2’-O-ribose methylation of spliceosomal small nuclear RNAs （snRNAs）. The snoRNA has two antisense elements, and the results of primer extensions at different dNTP concentrations provide evidence that snoR124 guide 2’-O-methylations of C93 in the U4 snRNA and the T87 residue in the U5 snRNA. In addition, this snoRNA is located in a snoRNA gene cluster with another 7 snoRNAs which are identified to direct ribose methylations in rRNAs. This is consistent with the opinion that the snoRNA gene organization in plant is mainly gene cluster. The snoR124 is the first example of a snoRNA that directs modifications of RNAs other than rRNAs in plant;it will avail to get more insights into the function of snoRNAs in plant.