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Dual RNA-seq provides novel insight into the roles of dks A from Pseudomonas plecoglossicida in pathogenhost interactions with large yellow croakers(Larimichthys crocea)

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Zoological Research 2020年第4期41卷 410-422页

作者： Lu-Ying Wang Zi-XuLiu Ling-Min Zhao Li-Xing Huang Ying-Xue Qin yong-Quan Su Wei-Qiang Zheng Fan Wang Qing-pi Yan Fisheries College Jimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker Breeding Ningde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extension Center FuzhouFujian 350003China

Pseudomonas plecoglossicida is a rod-shaped,gram-negative bacterium with *** causes visceral white spot disease and high mortality in Larimichthys crocea during culture,resulting in serious economic *** of transcriptome and quantitative real-time polymerase chain reaction(PCR)data showed that dks A gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides(log2 FC=3.12,P<0.001).RNAi of five sh RNAs significantly reduced the expression of dks A in ***,and the optimal silencing efficiency was 96.23%.Compared with wild-type strains,the symptoms of visceral white spot disease in *** infected with RNAi strains were reduced,with time of death delayed by 48 h and mortality reduced by 25%.The dks A silencing led to a substantial down-regulation in cellular component-,flagellum-,and ribosome assembly-related genes in ***,and the significant up-regulation of fli C may be a way in which virulence is maintained in *** GO and KEGG results showed that RNAi strain infection in *** led to the down-regulation of inflammatory factor genes in immune-related pathways,which were associated with multiple immune response *** also showed that dks A was a virulence gene in *** with the wild-type strains,RNAi strain infection induced a weaker immune response in ***.

关键词： mortality infection yellow

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人工智能刑事法治的基本问题

比较法研究 2018年第5期32卷 149-166页

作者：皮勇武汉大学法学院

人工智能将给社会带来重大变革,我国人工智能刑事法治面临新挑战,为此应树立正确的人工智能刑事法治理念,在维护人工智能的有益性发展的同时,保障合法权利,促进技术与行为规范的同步发展,全面规范人工智能产品与服务研发、提供和应用活... 详细信息

人工智能将给社会带来重大变革,我国人工智能刑事法治面临新挑战,为此应树立正确的人工智能刑事法治理念,在维护人工智能的有益性发展的同时,保障合法权利,促进技术与行为规范的同步发展,全面规范人工智能产品与服务研发、提供和应用活动。人工智能不应成为犯罪主体或者刑事责任主体。由于人工智能能替代部分人类智慧活动,其刑法地位有别于其他技术应用,引起新的刑法问题。人工智能犯罪是与人工智能系统研发、提供、应用和管理相关的犯罪,刑法应当对其进行全过程、全面惩治,不仅应惩治侵害人工智能系统安全犯罪和智能化的传统犯罪,还应惩治利用人工智能侵犯个人信息的犯罪、独立的外围人工智能犯罪和违反人工智能安全管理义务的犯罪。法律应跟随智能社会的发展与时俱进,人工智能的发展应用应坚持以法律为边界,避免偏离有益于人类社会发展进步的方向。

关键词：人工智能刑事法治理念犯罪主体地位人工智能犯罪

骨髓间充质干细胞移植治疗大鼠心肌梗死:急性和慢性运动的影响

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中国组织工程研究 2025年第23期29卷 4868-4877页

作者：冯强皮亦华黄华生黄德伦张艳广西中医药大学体育部广西壮族自治区南宁市530021 广西中医药大学基础医学院生理教研室广西壮族自治区南宁市530021

背景:干细胞移植对治疗心肌梗死具有可观的治疗前景,但移植细胞向心脏归巢效率及在心脏内的滞留率和存活率较低限制了干细胞疗效。运动疗法是心肌梗死患者心脏康复的重要有机组成部分,然而运动在干细胞移植治疗心肌梗死中的作用尚未明... 详细信息

背景:干细胞移植对治疗心肌梗死具有可观的治疗前景,但移植细胞向心脏归巢效率及在心脏内的滞留率和存活率较低限制了干细胞疗效。运动疗法是心肌梗死患者心脏康复的重要有机组成部分,然而运动在干细胞移植治疗心肌梗死中的作用尚未明确。目的:探讨运动(包括急性和慢性运动)对骨髓间充质干细胞移植治疗大鼠心肌梗死的影响。方法:取80只雌性SD大鼠,采用随机数字表法分为假手术组、模型组、移植组或联合组,每组20只。模型组、移植组或联合组大鼠通过冠状动脉结扎术制作心肌梗死模型,造模24 h后,联合组进行8周有氧运动(慢性运动,30 min/d,每周运动5 d),于首次运动(急性运动)后5 min内,移植组和联合组尾静脉输注绿色荧光蛋白标记的SD大鼠骨髓间充质干细胞,首次运动后24 h取部分大鼠,检测大鼠心肌内移植的干细胞存活率、Y染色体性别决定区mRNA表达、归巢因子蛋白表达以及氧化应激和炎症反应参数;末次运动后72 h取剩余大鼠,检测心脏结构与功能、心肌组织学变化和Ki67^(+)细胞数量。结果与结论:(1)急性运动后:与假手术组比较,模型组心肌活性氧、丙二醛、肿瘤坏死因子α和白细胞介素1β水平升高(P <0.05),超氧化物歧化酶活性降低(P <0.05);与模型组比较,移植组和联合组活性氧、丙二醛水平及肿瘤坏死因子α和白细胞介素1β蛋白表达降低(P <0.05),超氧化物歧化酶活性及基质细胞衍生因子1α、CXC趋化因子受体4蛋白表达升高(P <0.05);与移植组比较,联合组活性氧、丙二醛水平及肿瘤坏死因子α和白细胞介素1β蛋白表达降低(P <0.05),干细胞存活率、Y染色体性别决定区mRNA表达、超氧化物歧化酶活性及基质细胞衍生因子1α和CXC趋化因子受体4蛋白表达升高(P <0.05)。(2)慢性运动后:与假手术组比较,模型组心肌细胞横截面积、胶原含量增加(P <0.05),左心室射血分数、左心室短轴缩短率下降(P <0.05);与模型组比较,移植组心肌细胞横截面积与胶原含量降低(P <0.05),Ki67+细胞升高(P <0.05);与移植组比较,联合组胶原含量降低(P <0.05),心肌细胞横截面积、左心室射血分数、左心室短轴缩短率、Ki67+细胞升高(P <0.05)。(3)急性运动通过促进干细胞归巢、改善心肌微环境提高外源性干细胞存活率,慢性运动可刺激干细胞移植后心肌细胞增殖、抑制心脏重塑并增强心功能,提示运动有助于优化大鼠心肌梗死后干细胞移植疗效。

关键词：急性运动慢性运动干细胞移植骨髓间充质干细胞心肌梗死归巢

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论自动驾驶汽车生产者的刑事责任

比较法研究 2022年第1期36卷 55-70页

作者：皮勇同济大学上海国际知识产权学院

自动驾驶汽车是人类研发、制造、使用和管理的智能产品,不是犯罪主体或刑事责任主体。在自动驾驶汽车自主控制状态下发生交通事故的,其生产者、使用者和其他人员难以按照我国现有刑法的罪名定罪处罚。除非道路交通安全法和刑法有专门的... 详细信息

自动驾驶汽车是人类研发、制造、使用和管理的智能产品,不是犯罪主体或刑事责任主体。在自动驾驶汽车自主控制状态下发生交通事故的,其生产者、使用者和其他人员难以按照我国现有刑法的罪名定罪处罚。除非道路交通安全法和刑法有专门的规定,驾驶位人员不接管汽车或接管后无力改变交通事故结果的,不构成交通肇事罪或其他管理过失犯罪。驾驶位人员的注意义务是阻止自动驾驶汽车自主控制下发生交通事故,其注意义务不应过高。允许的风险、紧急避险理论不能为自动驾驶汽车紧急路况处理算法的生产与应用提供合法、合理的解决方案,生产者遵守算法安全标准仅可以使生产行为合法化。鉴于现行刑法不适应自动驾驶汽车应用的特性,我国应当建立以生产者全程负责为中心的新刑事责任体系,使之在自动驾驶汽车生产和应用两个阶段承担安全管理责任,生产者拒不履行自动驾驶汽车应用安全管理义务且情节严重的,应当承担刑事责任。

关键词：自动驾驶汽车行为性质注意义务算法决策新刑事责任体系

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论中国网络空间犯罪立法的本土化与国际化

比较法研究 2020年第1期34卷 135-154页

作者：皮勇武汉大学法学院武汉大学网络治理研究院

网络空间犯罪是计算机犯罪、网络犯罪在网络平台时代的新相态,为了有效打击网络空间犯罪,中国刑法既要坚持本土化发展,也要借鉴国外立法的成功经验,积极推动国际社会相关立法协调一致。计算机数据和信息系统安全是独立的公共法益,应当... 详细信息

网络空间犯罪是计算机犯罪、网络犯罪在网络平台时代的新相态,为了有效打击网络空间犯罪,中国刑法既要坚持本土化发展,也要借鉴国外立法的成功经验,积极推动国际社会相关立法协调一致。计算机数据和信息系统安全是独立的公共法益,应当给予全面的刑法保护。惩治网络化的传统犯罪应当坚持科学防控和法治原则,避免以刑代管,更不应突破法律规定解释立法。妨害信息网络安全管理秩序犯罪是独立的、具有"积量构罪"特征的正当立法,司法适用中应坚持独立适用和实质正当原则,合理缩限解释构成要件要素,对情节要件进行必要的类型化。充分保护个人信息符合中国国家利益、公共利益和公众利益,应在平衡个人信息法律保护和合法利用的基础上,为公民个人信息提供更全面的法律保护。

关键词：网络空间犯罪计算机数据及信息系统安全传统犯罪网络化妨害信息网络安全管理秩序犯罪个人信息

Novel insights into host-pathogen interactions of large yellow croakers(Larimichthys crocea)and pathogenic bacterium Pseudomonas plecoglossicida using time-resolved dual RNA-seq of infected spleens

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Zoological Research 2020年第3期41卷 314-327页

作者： Yi Tang Ge Xin Ling-Min Zhao Li-Xing Huang Ying-Xue Qin yong-Quan Su Wei-Qiang Zheng Bin Wu Nan Lin Qing-pi Yan Fisheries College Key Laboratory of Healthy Mariculture for the East China SeaMinistry of AgricultureJimei UniversityXiamenFujian 361021China State Key Laboratory of Large Yellow Croaker Breeding Ningde Fufa Aquatic Products Co.Ltd.NingdeFujian 352000China Fujian Provincial Fishery Technical Extention Center FuzhouFujian 350003China

Host-pathogen interactions are highly complex,involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, timeresolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection(dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study hostpathogen interactions between L. crocea and ***. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor,Toll-like receptor signaling, and other immunerelated pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune *** P. plecoglossicida, the expression levels of motility-related genes and flagellum assemblyrelated genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and ***.

关键词： Larimichthys crocea Pseudomonas plecoglossicida Dual RNA-seq Host-pathogen interactions Dynamic transcriptome

Extinction coefficients of surface atmospheric aerosol above LHAASO

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Chinese Physics C 2019年第8期43卷 117-123页

作者： Wen-Xuan pi Min Huang Feng-Rong Zhu Yu He Ning Xie yong Zhang Qi-Hui Chen Huan-Yu Jia School of Physical Science and Technology Southwest Jiaotong UniversityChengdu 611756China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

We investigate the extinction coefficients of the surface atmospheric aerosol over the Large High Altitude Air Shower Observatory (LHAASO), located at the Haizi Mountain, Daocheng County, China. To this end, we utilize the Longtin model, Mie scattering theory, and experimental data obtained by the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO). Our theoretical calculations show that the total extinction coefficients of the atmospheric aerosol at the wavelength of 200–500 nm are inversely proportional to the laser wavelength, and influenced by the wind speed. From July 2015 to October 2016, the extinction coefficient of the surface atmospheric aerosols at 532 nm wavelength reached 0.04 km^-1 with no wind, while it increased to 0.1 km^-1 with gusts. In this period, the extinction coefficients of the surface atmospheric aerosol at 532 nm wavelength, obtained by the CALIPSO, change from 0.01 to 0.07 km^-1, which is less than the values obtained the theoretical calculation and larger than the average of Tibetan Plateau in 2006-2016. These calculations and experimental evidence provide important arguments to the model of atmospheric aerosol to be applied in the calibration of LHAASO. Our results suggest that the extinction coefficients over LHAASO require further study, including research on the size distribution, shape, concentration of aerosols particles, wind dependence, relative humidity dependence, etc.

关键词： LHAASO extinction coefficient atmospheric aerosol

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网络黑灰产刑法规制实证研究

国家检察官学院学报 2021年第1期29卷 18-40页

作者：皮勇同济大学上海国际知识产权学院

网络黑灰产活动通常指称具有一定独立性、常业性的非法牟利型网络违法犯罪。利用对传统犯罪立法进行合理扩张解释的方法,能解决相当多网络黑灰产犯罪案件的法律适用问题,但也不应过度依赖扩张解释方法,对相关构成要件的解释应在合理限... 详细信息

网络黑灰产活动通常指称具有一定独立性、常业性的非法牟利型网络违法犯罪。利用对传统犯罪立法进行合理扩张解释的方法,能解决相当多网络黑灰产犯罪案件的法律适用问题,但也不应过度依赖扩张解释方法,对相关构成要件的解释应在合理限度内。当传统犯罪和侵犯公民个人信息罪不能规制网络黑灰产犯罪时,可以按手段行为入罪,适用侵犯计算机信息系统安全或妨害信息网络管理秩序相关罪名,后者对惩治新型网络黑灰产犯罪发挥了重要作用。当前侵犯计算机信息系统安全犯罪立法有不足之处,司法实践中存在泛化适用问题。妨害信息网络安全管理秩序犯罪是具有积量构罪构造的独立犯罪,不应以共犯或预备犯的构成条件来限制其适用。同时,为了避免司法实践中对其泛化适用,对相关构成要件的解释应当限制在合理范围内。

关键词：网络黑灰产合理扩张解释手段行为积量构罪实证研究

High-sensitivity phase imaging eddy current magneto-optical system for carbon fiber reinforced polymers detection

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Journal of Electronic Science and Technology 2023年第4期21卷 48-59页

作者： Jiang-Shan Ai Quan Zhou Yi-ping Liang Chun-rui Feng Bing Long Li-Bing Bai yong-Gang Wang Chao Ren School of Automation Engineering University of Electronic Science and Technology of ChinaChengdu611731China Department of Mechanical Engineering Catholic University of LeuvenLeuven3001Belgium Chengdu Aircraft Industry Company Ltd. Chengdu610091China

This paper proposed a high-sensitivity phase imaging eddy current magneto-optical (pi-ECMO) system for carbon fiber reinforced polymer (CFRP) defect detection. In contrast to other eddy current-based detection systems, the proposed system employs a fixed position excitation coil while enabling the detection point to move within the detection region. This configuration effectively mitigates the interference caused by the lift-off effect, which is commonly observed in systems with moving excitation coils. Correspondingly, the relationship between the defect characteristics (orientation and position) and the surface vertical magnetic field distribution (amplitude and phase) is studied in detail by theoretical analysis and numerical simulations. Experiments conducted on woven CFRP plates demonstrate that the designed pi-ECMO system is capable of effectively detecting both surface and internal cracks, as well as impact defects. The excitation current is significantly reduced compared with traditional eddy current magneto-optical (ECMO) systems.

关键词： Carbon fiber reinforced polymers Defect detection Eddy current magneto-optical Nondestructive testing Phase imaging