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The Crop Journal 2017年第5期5卷 438-447页

作者： Limin Liu Xiaomei Zhang fulu Chen Asia Adam Elzamzami Mahi Xiaoxia Wu Qingshan Chen yong-fu fu College of Agriculture Northeast Agricultural University MOA Key Lab of Soybean Biology (Beijing) National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences

Regulation of flowering is one of the key issues in crop yield. The Floiuering Loews T(FT) gene is a well-known florigen, which integrates various signals from multiple flowering-regulation pathways to initiate flowering. We previously reported that there are at least six FT genes(GmFTLl-6) in soybean displaying flowering activity. However, the individual functions of genes GmFTLl-6 remain to be identified. In this study, we cloned the GmFTL2 promoter(GmFTLpro) from soybean(Glycine max) cultivar Tianlong 1 and analyzed its motifs bioinformatically and its expression patterns using both a transgenic approach and quantitative RT-PCR(qRT-PCR). In GmFTLpro::GUS transgenic lines, GUS signals were enriched in cotyledons, hypocotyledons, pollen, embryos, and root tips in a photoperiod-independent manner. qRT-PCR confirmed the GUS reporter results. Our results suggest that GmFTL2 expression is regulated by developmental and tissue-specific clues and plays roles in seedling establishment and the development of micro game tophytes, embryos, and roots.

关键词： Flowering Locus T Cotyledon Microgametophyte Embryo Root tip

A callus transformation system for gene functional studies in soybean

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Journal of Integrative Agriculture 2017年第9期16卷 1913-1922页

作者： XU Kun ZHANG Xiao-mei FAN Cheng-ming CHEN fu-lu ZHU Jin-long ZHANG Shi-long CHEN Qing-shan fu yong-fu Key Lab of Soybean Biology(Beijing) Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Bijie Agricultural Science Research Institute of Guizhou Bijie 551700P.R.China College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China

Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants（e.g., soybean）, which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.

关键词： soybean callus gene function studies transformation

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拟南芥SUMO底物的研究进展

遗传 2013年第6期35卷 727-734页

作者：郭敏霞傅永福中国农业科学院作物科学研究所农业部北京大豆生物学重点实验室农作物基因资源与遗传改良国家重大科学工程北京100081

SUMO(Small ubiquitin-related modifier)化修饰是普遍存在于真核生物中的一种翻译后修饰,在很多细胞过程中发挥重要作用。文章阐述了拟南芥中SUMO底物的最新研究进展。首先介绍SUMO化修饰过程,SUMO底物的鉴定,包括鉴定方法的进展和鉴... 详细信息

SUMO(Small ubiquitin-related modifier)化修饰是普遍存在于真核生物中的一种翻译后修饰,在很多细胞过程中发挥重要作用。文章阐述了拟南芥中SUMO底物的最新研究进展。首先介绍SUMO化修饰过程,SUMO底物的鉴定,包括鉴定方法的进展和鉴定成果。然后依据底物的亚细胞定位、生理功能和参与的生理生化过程对其进行分类和分析,以期更好地理解蛋白质SUMO化修饰方式在植物生长发育中的功能。

关键词： SUMO化修饰底物鉴定翻译后修饰拟南芥

GmDRR1,a dirigent protein resistant to Phytophthora sojae in Glycine max (L.) Merr.

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Journal of Integrative Agriculture 2018年第6期17卷 1289-1298页

作者： CHEN Qing-shan YU Guo-long ZOU Jia-nan WANG Jing QIU Hong-mei ZHU Rong-sheng CHANG Hui-lin JIANG Hong-wei HU Zhen-bang LI Chang-yu ZHANG Yan-jiao WANG Jin-hui WANG Xue-ding GAO Shan LIU Chun-yan QI Zhao-ming fu yong-fu XIN Da-wei Key Laboratory of Soybean Biology Ministry of Education/College of AgricultureNortheast Agricultural University Key Laboratory of Soybean Biology (Beijing) Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural Sciences

Soil-borne pathogen Phytophthora sojae is an oomycete that causes devastating damage to soybean yield. To mine original resistant genes in soybean is an effective and environmentally-friend approach controlling the disease. In this study, soybean proteins were extracted from the first trifoliolates infected by predominant P. sojae race 1 and analyzed by twodimensional gel electrophoresis. Nineteen differently-expressed protein spots were detected, and 10 of them were further applied for Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry Assay. One protein containing a dirigent （DIR） domain was identified and belonged to the DIR-b/d family. Therefore, it was named as GmDRR1 （Glycine max Disease Resistance Response 1）. Then, GmDRR1 gene was pathologically confirmed to be involved in the resistant to P. sojae in soybean. GmDRR1-GFP （green fluorescent protein） fusion proteins localized in the cell membrane. qRTPCR results showed GmDRR1 gene expressed differently in P. sojae resistant- and susceptible-soybean cultivars. By the promoter analysis, we found a haplotype H8 was existing in most resistant soybean varieties, while a haplotype H77 was existing in most susceptible soybean varieties. The H77 haplotype had seven SNPs （C to A, G to C, C to A, T to A, T to C, T to C, and T to A） and two single nucleotide insertions. The results supported that the expression difference of GmDRR1 genes between P. sojae resistant- and susceptible-soybean cultivars might depend on the GmDRR1 promoter SNPs. The results suggested that GmDRR1 was a dirigent protein involved in soybean resistant to P. sojae and paved a novel way for investigation of the molecular regulatory mechanism of the defense response to P. sojae in soybean.

关键词： dirigent domain GmDRR1 soybean Phytophthora sojae oomycete proteomics

Imperative for long-term management and surveillance in Kawasaki disease

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World Journal of Clinical Cases 2025年第4期13卷 61-63页

作者： Yan Pan fu-yong Jiao Department of Pediatrics The First Affiliated Hospital of Yangtze UniversityJingzhou 434000Hubei ProvinceChina Shaanxi Kawasaki Disease Diagnosis and Treatment Center Children’s HospitalShaanxi Provincial People’s Hospital of Xi’an Jiaotong UniversityXi’an 710000Shaanxi ProvinceChina

Kawasaki disease(KD)is a significant pediatric vasculitis known for its potential to cause severe coronary artery *** the effectiveness of initial treatments,such as intravenous immunoglobulin,KD patients can experience long-term cardiovascular issues,as evidenced by a recent case report of an adult who suffered a ST-segment elevation myocardial infarction due to previous KD in the World Journal of Clinical *** editorial emphasizes the critical need for long-term management and regular surveillance to prevent such *** drawing on recent research and case studies,we advocate for a structured approach to follow-up care that includes routine cardiac evaluations and preventive measures.

关键词： Kawasaki disease Long-term management Coronary artery aneurysm Surveillance Preventive care

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拟南芥SUA41基因的表达和功能分析

遗传 2013年第1期35卷 93-100页

作者：黄国文韩玉珍傅永福湖南科技学院生命科学和化学工程学院永州425100 中国农业大学生物学院北京100193 农业部北京大豆生物学重点实验室农作物基因资源与遗传改良国家重大科学工程中国农业科学院作物科学研究所北京100081

植物的开花受多条途径的控制,其中包括光周期途径、春化途径、赤霉素途径、自主途径和温敏途径。SUA41(SUMO substrate in Arabidopsis 41)是本实验室筛选到的、SUMO(small ubiquitin modifier)的潜在底物,并且前人的研究发现它参与自... 详细信息

植物的开花受多条途径的控制,其中包括光周期途径、春化途径、赤霉素途径、自主途径和温敏途径。SUA41(SUMO substrate in Arabidopsis 41)是本实验室筛选到的、SUMO(small ubiquitin modifier)的潜在底物,并且前人的研究发现它参与自主途径的开花调节,但其对开花时间的调节机制没有详细报道。文章对SUA41基因的表达、sua41突变体对不同环境条件的反应以及SUA41对开花时间调节的可能机制进行初步分析。结果显示,与野生型相比,sua41突变体在常温或低温、长日或者短日条件下均为早花,并且在低温和常温下的开花时间没有太大差别。过表达SUA41能够恢复sua41突变体的早花表型。SUA41基因在拟南芥的幼苗、根、茎、叶和花以及各个植物发育阶段都有表达,说明SUA41基因是一个组成型表达基因。SUA41基因的表达与GA处理无关,长日低温条件能够诱导SUA41基因的表达,且在温敏途径突变体fve和fca中SUA41基因的表达量减少。与野生型比较,sua41突变体中CO基因的mRNA表达量没有明显变化,FT和SOC1基因表达量增加且FT增加幅度更大,FLC的mRNA表达量减少。结果表明SUA41基因虽然在自主途径中起作用,但主要在温敏途径中参与拟南芥开花时间调节。

关键词：开花调节表达分析自主途径温敏途径

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大豆GmNF-YC2基因的克隆与功能分析

作物学报 2012年第9期38卷 1607-1616页

作者：曹岩张晓玫陈新建傅永福河南农业大学生命科学学院河南郑州450002 农业部北京大豆生物学重点实验室农作物基因资源与遗传改良国家重大科学工程/中国农业科学院作物科学研究所北京100081

NF-Y(nuclear factor-y)是真核生物中普遍存在的一种转录因子复合物,由3个不同的亚基(NF-YA、NF-YB和NF-YC)组成,并通过作用于其他调控因子的启动子来调节目的基因的表达。本文从大豆垦农18中克隆大豆NF-YC基因GmNF-YC2,并且利用实时荧... 详细信息

NF-Y(nuclear factor-y)是真核生物中普遍存在的一种转录因子复合物,由3个不同的亚基(NF-YA、NF-YB和NF-YC)组成,并通过作用于其他调控因子的启动子来调节目的基因的表达。本文从大豆垦农18中克隆大豆NF-YC基因GmNF-YC2,并且利用实时荧光定量PCR(qRT-PCR)分析其表达模式,发现GmNF-YC2的表达受光调控,并在开花期第3复叶中表达量最高。结果表明,GmNF-YC2在大豆光周期开花调节中起重要作用。拟南芥原生质体瞬时表达实验证实,GmNF-YC2蛋白定位在细胞核中。这为大豆NF-YC家族基因的功能研究提供重要依据。

关键词： NF—Y转录因子开花 CONSTANS 光周期大豆

拟南芥核膜孔蛋白Nup160基因的突变体表型分析

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中国农业科技导报 2015年第3期17卷 29-34页

作者：刘文瑄沈应柏傅永福北京林业大学生物科学与技术学院林木育种国家工程实验室北京100083 中国农业科学院作物科学研究所国家农作物基因资源与遗传改良重大科学工程农业部北京大豆生物学重点实验室北京100081

植物开花受环境条件的影响,其分子调控机制在转录、翻译和表观遗传等层次已有较广泛而深入的研究;核膜孔复合物是真核细胞中细胞质与细胞核之间物质交换的重要通道。筛选到拟南芥核膜孔蛋白Nup(Nucleoporin)160基因的3个突变体nup160-1/... 详细信息

植物开花受环境条件的影响,其分子调控机制在转录、翻译和表观遗传等层次已有较广泛而深入的研究;核膜孔复合物是真核细胞中细胞质与细胞核之间物质交换的重要通道。筛选到拟南芥核膜孔蛋白Nup(Nucleoporin)160基因的3个突变体nup160-1/-2/-3,均表现出发育的多种表型,最显著的表型为早花,而且是不依赖于环境条件(光周期和温度)的早花。但是,突变体在长日下的开花时间依然早于短日条件下的开花时间,暗示突变体仍然对光周期有反应。结果表明,在拟南芥中Nup160作为一个植物开花的负调控因子,主要在温敏途径和光周期途径中参与拟南芥开花时间的调节。

关键词：核膜孔蛋白 Nup160突变体温敏途径光周期途径早花

Two Nucleoporin 98 homologous genes jointly participate in the regulation of senescence and flowering in Arabidopsis

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Two Nucleoporin 98 homologous genes jointly participate in t...

Energy Method in the Safety Assessment of a Building under Workshop Vibration

第三届全国植物开花·衰老与采后生物学大会

作者： Long Xiao Shanshan Jiang Penghui Huang fulu Chen Chunyang Liu Xiaoxia Wu Qingshan Chen Xiao-Mei Zhang yong-fu fu College of Agriculture Northeast Agricultural University Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Flowering and senescence are two important and closely related plant development events and regulated by a complicated network. In this study, we present findings that Nucleoporin 98（Nup98） may participate in regulation of both senescence and flowering in Arabidopsis thaliana. We found that Nup98a and Nup98b redundantly function in the early steps of starch degradation, the nup98a nup98b double mutant accumulates more starch than wild type and has a severe early senescence phenotype compared to wild type. The results suggest a role for Nup98 in starch degradation conferring to growth regulation in Arabidopsis. On the other hand, the nup98a nup98b double mutant also displays early flowering. Significantly, Nup98a and Nup98b gate flowering in a CONSTANS（CO）-independent mode and the nup98a nup98b co triple mutant remains early flowering similar to the nup98a nup98b double mutant. But the ft mutant can rescue the early flowering phenotype and the nup98a nup98b ft triple mutant flower late. Therefore, Nup98a and Nup98b bypass the CO checkpoint in photoperiodic signaling and integrated signals from other flowering pathways to directly target FLOWERING LOCUS T（FT） for flowering control. What is more, the ft mutant cannot rescue early senescence phenotype of the nup98a nup98b mutant because the nup98a nup98b ft triple mutant still has senescence characters as the nup98a nup98b double mutant does. Our results provide a line of evidence for uncoupling the mechanism of flowering and senescence at Nup98a and Nup98b genes in Arabidopsis, which are classically recognized as two coupled developmental events.

关键词： Nucleoporin 98 Flowering Senescence Starch FLOWERING LOCUS T Arabidopsis

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International Journal of Plant Engineering and Management 2002年第1期7卷 39-44页

作者： SUN yong 1, fu Ming fu 1, YANG Guo tai 1, HUANG Ju hua 1, ZHANG Ming hui 2, 1 Nanchang University, 330029 2 Jiangxi Medical Institute, 330006 Nanchang University 3300292 Jiangxi Medical Institute 330006

A Forging machine with its vibration isolation rig, its foundation, and the workshop building constitute a complex non linear dynamic system. The vibration caused by forging, propagating through the ground, will seriously affect the safety of the workshop building. A new kind of energy method is developed for the safety assessment of a building under workshop vibration.

关键词： workshop vibration safety assessment energy method