A callus transformation system for gene functional studies in soybean

作     者：XU Kun ZHANG Xiao-mei FAN Cheng-ming CHEN Fu-lu ZHU Jin-long ZHANG Shi-long CHEN Qing-shan FU Yong-fu 

作者机构：Key Lab of Soybean Biology(Beijing)Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Bijie Agricultural Science Research Institute of GuizhouBijie 551700P.R.China College of AgricultureNortheast Agricultural UniversityHarbin 150030P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2017年第16卷第9期

页      面：1913-1922页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the Transgenic Programs,China(2014ZX0800930B and 2016ZX08009-001) the National Natural Science Found of China(31371703) 

主　　题：soybean callus gene function studies transformation 

摘      要：Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants（e.g., soybean）, which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.