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Genetic analysis and ca.didate gene identifica.ion of salt tolerancerelated traits in maize

Journal of Integrative Agriculture 2024年第7期23卷 2196-2210页

作者： Hui Fang Xiuyi Fu Hanqiu Ge Mengxue Jia Jie Ji yizhou Zhao Zijian Qu Ziqian Cui Aixia Zhang Yuandong Wang Ping Li Baohua Wang Scientific Observing and Experimental Station of Maize in Plain Area of Southern Region Ministry of Agriculture and Rural Affairs/School of Life SciencesNantong UniversityNantong 226019China Maize Research Institute Beijing Academy of Agriculture and Forestry Sciences(BAAFS)Beijing 100097China

Soil salinization poses a threat to maize production worldwide,but the genetic mechanism of salt tolerance in maize is not well ***,identifying the genetic components underlying salt tolerance in maize is of great *** the current study,a teosinte-maize BC2F7 population was used to investigate the genetic basis of 21 salt tolerance-related *** total,125 QTLs were detected using a high-density genetic bin map,with one to five QTLs explaining 6.05–32.02%of the phenotypic variation for each *** total phenotypic variation explained(PVE)by all detected QTLs ranged from 6.84 to 63.88%for each *** all 125 QTLs,only three were major QTLs distributed in two genomic regions on chromosome 6,which were involved in three salt tolerance-related *** addition,10 pairs of epistatic QTLs with additive effects were detected for eight traits,explaining 0.9 to 4.44%of the phenotypic ***,18 QTL hotspots affecting 3–7 traits were *** one hotspot(L5),a gene cluster consisting of four genes(ZmNSA1,SAG6,ZmCLCg,and ZmHKT1;2)was found,suggesting the involvement of multiple pleiotropic ***,two important ca.didate genes,Zm00001d002090 and Zm00001d002391,were found to be associated with salt tolerance-related traits by a combination of linkage and marker-trait association ***00001d002090 encodes a ca.cium-dependent lipid-binding(ca.B domain)family protein,which may function as a ca.(2+)sensor for transmitting the salt stress signal downstream,while Zm00001d002391 encodes a ubiquitin-specific protease belonging to the C19-related *** findings provide valuable insights into the genetic basis of salt tolerance-related traits in maize and a theoretica. foundation for breeders to develop enhanced salt-tolerant maize varieties.

关键词： maize salt tolerance-related traits QTL mapping region-based association analysis

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Overexpression of tonoplast ca.(2+)-ATPase in guard cells synergistica.ly enhances stomatal opening and drought tolerance

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Journal of Integrative Plant Biology 2024年第8期66卷 1587-1602页

作者： Jinghan Su Bingqing He Peiyuan Li Baiyang Yu Qiwen Cen Lingfeng Xia Yi Jing Feibo Wu Rucha Karnik Dawei Xue Michael R.Blatt yizhou Wang Institute of Crop Science Zhejiang UniversityHangzhou 310058China Laboratory of Plant Physiology and Biophysics University of GlasgowGlasgow G128QQUK BGI Research Sanya 572025China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhou 311121China Zhejiang Provincial Key Laboratory of Crop Germplasm Zhejiang UniversityHangzhou 310058China

Stomata play a crucial role in plants by controlling water status and responding to drought ***,simultaneously improving stomatal opening and drought tolerance has proven to be a significa.t *** address this issue,we employed the OnGuard quantitative model,which accurately represents the mechanics and coordination of ion transporters in guard *** the guidance of OnGuard,we successfully engineered plants that overexpressed the main tonoplast ca.(2+)-ATPase gene,Aca.1,which promotes stomatal opening and enhances plant ***,these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their ***,OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the Aca.1 overexpressing *** study revealed that the overexpression of Aca.1 facilitated the accumulation of ca.(2+)in the vacuole,thereby influencing ca.(2+)storage and leading to an enhanced ca.(2+)elevation in response to abscisic *** regulatory ca.ca.e finely tunes stomatal responses,ultimately leading to enhanced drought *** findings underscore the importance of tonoplast ca.(2+)-ATPase in manipulating stomatal behavior and improving drought ***,these results highlight the diverse functions of tonoplast-loca.ized Aca.1 in response to different conditions,emphasizing its potential for future applica.ions in plant enhancement.

关键词： Aca.1 drought guard cells modeling stomata

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二维范德华磁体的拓扑自旋结构的研究进展

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Science Bulletin 2024年第11期69卷 1612-1616页

作者：许洪军刘艺舟 Giovanni Finocchio Kang LWang 于国强 Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100190China RIKEN Center for Emergent Matter Science(CEMS) Wako 351-0198Japan Department of Mathematica. and Computer Sciences Physical Sciences and Earth SciencesUniversity of MessinaMessina 98166Italy Department of Electrica. Engineering University of CaliforniaLos Angeles CA 90095USA

The importance of topologica. spin textures(TSTs), such as skyrmions, merons, hopfions, etc., is due to their static and dynamic properties [1]. They ca.ry a topologica. number that characterizes the homotopy group Π_(n)(S^(2))(n ∈ Z) and classifies maps from S^(n) to S^(2)(e.g., the skyrmion winding number corresponds to Π_(2)(S^(2))).

关键词：自旋结构 topologica. homotopy

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A Rapid Synthesis of High Aspect Ratio Silver Nanowires for High-Performance Transparent Electrodes

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中国化学:英文版 2015年第1期33卷 147-151页

作者： Tao Cheng yizhou Zhang Wenyong Lai Yao Chen Wei Huang Key Laboratory for Organic Electronics&Information Displays(KLOEID) Institute of Advanced Materials(IAM)Nanjing University of Posts and TelecommunicationsNanjingJiangsu 210023China

A rapid,simple and cost-effective polyol method has been developed for the synthesis of silver nanowires with high aspect ratio and high *** aspect ratios of the silver nanowires as high as ca.1000(average length 40μm and some even as long as 80μm,diameter 50-100 nm)were obtained via optimizing the reaction *** electrodes with excellent optoelectronic performances(optica. transmittance of 90%,sheet resistance of 23.2Ω/□and optica. transmittance of 87%,sheet resistance of 19.7Ω/□)comparable to commercial ITO were fab-rica.ed via simple spin coating the resulting silver nanowires onto the glass *** high optoelectronic per-formances and the facile all-solution process of the as-prepared transparent electrodes render them rather promising ca.didates for use in cost-effective large-area optoelectronic devices.

关键词： silver nanowires transparent electrodes high aspect ratio all-solution process large-area electronics

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Assessment of the Tidal Current Energy Resources and the Hydrodynamic Impacts of Energy Extraction at the PuHu Channel in Zhoushan Archipelago,China

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Journal of Ocean University of China 2021年第3期20卷 478-488页

作者： WU He YU Huaming FANG yizhou ZHOU Qingwei ZHUO Fengxuan KELLY Ryan M. National Ocean Technology Center Tianjin 300112China College of Oceanic and Atmospheric Sciences Ocean University of ChinaQingdao 266100China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China Rykell Scientific Editorial PlacentiaCA 92871USA

An unstructured model FVCOM(The Unstructured Grid Finite Volume Community Ocean Model)with sink momentum term was applied to simulate the tidal current field in Zhoushan Archipelago,China,with focus on the region named PuHu Channel between Putuo Island and Hulu *** model was ca.ibrated with several measurements in the channel,and the model perform-ance was *** examination of the spatial and temporal distributions of tidal energy resources based on the numerica. simula-tion revealed that the greatest power density of tidal energy during spring tide is 3.6kWm^(−2)at the northern area of the *** parameters were introduced to characterize the generation duration of the tidal array that ca.ses the temporal variation of tidal current *** annual average available energy in the channel was found to be approximately *** annual generating hours at rated power was found to be 1800 h when the installed ca.acity of tidal array is approximately 12MW.A site for the tidal array with 25 turbines was selected,and the layout of the array was configured based on the EMEC *** influence due to the deployment of the tidal array was simulated by the modified FVCOM *** simulation showed that the tidal level did not significa.tly change beca.se of the operation of the tidal *** velocity reduction covered a 2km^(2)area of the downstream the tidal array,with a maximum velocity reduction of 8cms−1 at mid-flood tide,whereas the streamwise velocity on both sides of the farm increased slightly.

关键词： tidal current energy resources assessment numerica. simulation hydrodynamic effects

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Effects of palmatine on potassium and ca.cium currents in isolated rat hepatocytes

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World Journal of Gastroenterology 2003年第2期9卷 329-333页

作者： FangWang Hong-yizhou LanCheng GangZhao JiZhou Li-YingFu Wei-XingYao DepartmentofPharmacology TongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina DepartmentofPancreaticSurgeryCenter UnionHospitalTongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430022HubeiProvinceChina

AIM: To study the effects of palmatine, a known inhibitoron delayed rectifier potassium current and L-type ca.ciumcurrent (Ica.L) in guinea pig ventricular myocytes, on thepotassium and ca.cium currents in isolated rat ***: Tight-seal wh ole-cell patch-clamp techniqueswere performed to investigate the effects of palmatine onthe delayed outward potassium currents (IK), inward rectifierpotassium current (IK1) and ca.+ release-activated ca.+current (ICRAC) in enzymatica.ly isolated rat ***: Palmatine 0.3-100 μM reduced IK in a concentationdependent manner with EC50of 41.62±10.11 μM and nH,0.48±0.07 (n=8). The effect of the drug was poorly reversibleafter washout. When the bath solution was changed totetraethylammonium (TEA) 8 mM, IK was *** 10 μM and 100 μM shifted the I-V curves of IKdownward, and the block of IK was *** 0.3-100 μM also inhibited ICRAC in a concentration-dependent manner. The fitting parameters were as follows:ECs0=51.19±15.18 μM, and nH=0.46+0.07 (n=8). The peakvalue of ICRAC in the I-V relationship was decreased bypalmatine 10 μM and 100 μM. But the reverse potential ofIcRAcoCcurred at Voltage=0 mV in all cells. Palmatine 0.3-100 μM failed to have any significa.t effect on either inwardor outward components of IK1 at any membrane ***: The inhibitory effects on IK and ICRAC couldbe one of the mechanisms that palmatine exerts protectiveeffect on hepatocytes.

关键词：掌叶防己碱肝实质细胞钙离子电流钾离子电流细胞培养作用机制

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Inhibitory effects of berberine on ion channels of rat hepatocytes

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World Journal of Gastroenterology 2004年第19期10卷 2842-2845页

作者： FangWang Hong-yizhou GangZhao Li-YingFu LanCheng Jian-GuoChen Wei-XingYao DepartmentofPharmacology TongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina PancreaticSurgeryCenter UnionHospitalTongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430022HubeiProvinceChina

AIM: To examine the effects of berberine, an isoquinoline alkaloid with a long history used as a tonic remedy for liver and heart, on ion channels of isolated rat ***: Tight-seal whole-cell patch-clamp techniques were performed to investigate the effects of berberine on the delayed outward potassium currents (Ik), inward rectifier potassium currents (Ik1) and ca.2+ release-activated ca.+ currents (ICRAC) in enzymatica.ly isolated rat ***: Berbenne 1-300 μmol/L reduced/K in a concentration-dependent manner with EC50 of 38.86=1=5.37 μmol/L and nH of 0.82±0.05 (n = 8). When the bath solution was changed to tetraethylammonium (TEA) 8 retool/L,IK was *** 30 μmol/L reduced/K at all examined membranepote ntials, especially at potentials positive to +60 mV (n = 8,Pca.cium currents in isolated rat hepatocytes, which may be involved in hepatoprotection.

关键词：抑制作用黄连素离子通路老鼠肝细胞钾离子

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经颅重复针刺对血管性痴呆模型大鼠认知功能及海马突触超微结构的影响

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上海针灸杂志 2024年第1期43卷 89-95页

作者：于国强孙婧妍关莹唐祎周杨添淞石光煜冯秋菊曹燚吴壮张良李红伟黑龙江中医药大学附属第二医院哈尔滨150001 黑龙江中医药大学哈尔滨150040 黑龙江中医药大学附属第一医院哈尔滨150040 孙申田名老中医工作室哈尔滨150006

目的观察经颅重复针刺对血管性痴呆(vascular dementia, VaD)大鼠认知功能及海马ca.区突触素(synaptophysin, SYN)、微管相关蛋白-2(microtubule associated protein-2, MAP-2)表达的影响。方法将56只SD大鼠随机分为空白组(14只)和造模组(42只)。造模组建立VaD模型,将造模成功大鼠随机分为模型组(14只)、常规针刺组(14只)和经颅重复针刺刺激(repetitive transcranial acupuncture stimulation, rTAS)组(14只)。造模成功后第2天,rTAS组采用经颅重复针刺;常规针刺组仅给予常规针刺手法;模型组与空白组给予以同等条件抓取及固定,不予针刺。采用水迷宫评价大鼠学习记忆能力,采用旷场实验评价大鼠探索行为和自主活动能力,免疫印迹法检测海马ca.区SYP、MAP-2蛋白表达水平,透射电镜观察海马组织中突触超微结构。结果与空白组比较,模型组逃避潜伏时间明显增加(P0.05)。与空白组比较,模型组穿越格子数与后肢站立数明显减少(P<0.05);与模型组比较,常规针刺组与rTAS组穿越格子数与后肢站立数明显增加(P<0.05);与常规针刺组比较,rTAS组穿越格子数与后肢站立数明显增加(P<0.05)。与空白组比较,模型组SYP、MAP-2表达水平显著降低(P<0.05);与模型组比较,常规针刺组与rTAS组SYP、MAP-2表达水平显著增高(P<0.05);与常规针刺组比较,rTAS组SYP、MAP-2表达水平显著增高(P<0.05)。电镜下,模型组突触结构欠清晰,突触间隙模糊不清,突触小泡较少,突触前致密物质减少,胞浆内细胞器稀疏,线粒体变形肿大明显;而rTAS组突触结构较完整,突触间隙、突触前后膜分界清晰,突触前膜内囊泡较多,突触后膜均匀增厚,周边线粒体丰富、结构完整。结论经颅重复针刺较常规针刺可能通过调控SYP、MAP-2表达,更有效促进突触再生,改善突触可塑性,修复受损神经元,从而改善认知功能。

关键词：针刺疗法经颅重复针刺痴呆,血管性突触海马认知功能大鼠

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Results of robot-assisted minimally invasive esophagectomy in 111 consecutive patients:an Asia cohort study

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Holistic Integrative Oncology 2023年第1期2卷 291-299页

作者： Shuliang Zhang yizhou Huang Maohui Chen Taidui Zeng Guanglei Huang Wei Zheng Wei Li Chun Chen Bin Zheng Fujian Key Laboratory of ca.diothoracic Surgery(Fujian Medica. University) Fuzhou 350001FujianChina Department of Thoracic Surgery Fujian Medical University Union HospitalNo.29Xinquan RoadFuzhou 350001FujianChina

Background Robot-assisted minimally invasive esophagectomy(RAMIE)is becoming increasingly popular as a treatment for esophageal *** purpose of this study was to use the da Vinci robotic system(Intuitive Surgica.,Sunnyvale,ca.USA)to perform 111 consecutive ca.es of radica. esophageal ca.cer and report the perioperative data of surgery and postoperative complica.ions and short-term oncologica. *** We retrospectively analyzed 111 patients who underwent RAMIE conducted at Fujian Medica. University Union Hospital from August 2016 to January *** patient’s characteristics,clinicopathologica. stage,postoperative pathologica. stage,surgery outcome,postoperative recovery,and short-term oncologica. outcomes were *** Of the 111 patients who underwent RAMIE,77 were male and 34 were female,and the mean age was 62.1±8.8 ***-seven patients(24.3%)received preoperative neoadjuvant therapies,the most popular of which was preoperative induction chemotherapy in 16 ca.es(14.4%),followed by preoperative induction radiotherapy in 8 ca.es(7.2%)and preoperative induction chemotherapy plus immunization in 3 ca.es(2.7%).The vast majority of patients(110/111,99.1%)underwent radica. resection,with a mean intraoperative bleeding amount of 99.9±68.4 mL and a mean operative time of 271.9±70.0 *** mean total number of lymph nodes removed was 40.9±16.7,including 21.8±9.0 thoracic lymph ***-five(49.6%)patients had lymph node metastases,including 17(15.3%)with lymph node metastases in the left recurrent laryngeal nerve,24(21.6%)with lymph node metastases in the right recurrent laryngeal nerve,and 7(6.3%)with lymph node metastases in the bilateral recurrent laryngeal *** positive rate of left recurrent nerve lymph nodes was 4.77%,and he positive rate of right recurrent nerve lymph nodes was 8.38%.The main postoperative complica.ions included pulmonary infection in 24 ca.es(21.6%),celiac disease in 3 ca.es(2.7%),tracheoesophageal fistula i

关键词： Robot-assisted surgery esophageal ca.cer Minimally invasive surgery Complica.ions Short-term outcomes

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Rapid actions of androgen on PSD95 expression mediated by ca.(2+)/ca.KI pathway in HT22 cells

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解剖学杂志 2021年第S1期44卷 77-78页

作者： Mi Shixiong Li Sha Zhang yizhou Chen Meiqin Du J uan Wang Chang Ma Min Cui Huixian Department of Anatomy Hebei Medical UniversityShijiazhuang 050017China

The rapid actions of androgen on synaptic plasticity have been extensively ***,the underlying mechanisms remain *** this study,we used mouse hippoca.pal neuron cell line HT22 cells as a cell research model to investigate whether ca.(2+)/ca.KII mediates androgen rapid actions on synaptic plasticity,especially for synaptic protein PSD95 *** ca.cium imaging,we verified incubation with 100 nmol/L testosterone for 20 min had the most significa.t effect on intracellular ca.(2+)*** addition,the depolarization of resting membrane potential after testosterone stimulation was observed by patch *** found that the depolarization of resting membrane potential was to-40 mV.

关键词： expression. stimulation PSD95

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