Effects of palmatine on potassium and calcium currents in isolated rat hepatocytes
Effects of palmatine on potassium and calcium currents in isolated rat hepatocytes作者机构：DepartmentofPharmacologyTongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina DepartmentofPancreaticSurgeryCenterUnionHospitalTongjiMedicalCollegeofHuazhongUniversityofScienceandTechnologyWuhan430022HubeiProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2003年第9卷第2期
页 面：329-333页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床]
主 题：掌叶防己碱 肝实质细胞 钙离子电流 钾离子电流 细胞培养 作用机制
摘 要：AIM: To study the effects of palmatine, a known inhibitoron delayed rectifier potassium current and L-type calciumcurrent (ICa,L) in guinea pig ventricular myocytes, on thepotassium and calcium currents in isolated rat ***: Tight-seal wh ole-cell patch-clamp techniqueswere performed to investigate the effects of palmatine onthe delayed outward potassium currents (IK), inward rectifierpotassium current (IK1) and Ca2+ release-activated Ca2+current (ICRAC) in enzymatically isolated rat ***: Palmatine 0.3-100 μM reduced IK in a concentationdependent manner with EC50of 41.62±10.11 μM and nH,0.48±0.07 (n=8). The effect of the drug was poorly reversibleafter washout. When the bath solution was changed totetraethylammonium (TEA) 8 mM, IK was *** 10 μM and 100 μM shifted the I-V curves of IKdownward, and the block of IK was *** 0.3-100 μM also inhibited ICRAC in a concentration-dependent manner. The fitting parameters were as follows:ECs0=51.19±15.18 μM, and nH=0.46+0.07 (n=8). The peakvalue of ICRAC in the I-V relationship was decreased bypalmatine 10 μM and 100 μM. But the reverse potential ofIcRAcoCcurred at Voltage=0 mV in all cells. Palmatine 0.3-100 μM failed to have any significant effect on either inwardor outward components of IK1 at any membrane ***: The inhibitory effects on IK and ICRAC couldbe one of the mechanisms that palmatine exerts protectiveeffect on hepatocytes.
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