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Diversity and connectedness of brine shrimp viruses in global hypersaline ecosystems

Science China(Life Sciences) 2024年第1期67卷 188-203页

作者： Xuan Dong Cixiu Li yiting Wang Tao Hu Fan zh.ng Fanzeng Meng Meirong Gao Xuekai Han Guohao Wang Jiahao Qin Hans Nauwynck Edward CHolmes Patrick Sorgeloos Liying Sui Jie Huang Weifeng Shi State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesLaboratory for Marine Fisheries Science and Food Production ProcessesLaoshan LaboratoryKey Laboratory of Maricultural Organism Disease ControlMinistry of Agriculture and Rural AffairsQingdao Key Laboratory of Mariculture Epidemiology and BiosecurityQingdao 266071China Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan 250021China Key Laboratory of Emerging Infectious Diseases in Universities of Shandong Shandong First Medical University&Shandong Academy of Medical SciencesTaian 271000China Dalian Ocean University Dalian 116023China Asian Regional Artemia Reference Center Tianjin University of Science&TechnologyTianjin 300457China Ghent University Ghent 9000Belgium Sydney Institute for Infectious Diseases School of Medical SciencesThe University of SydneySydney 2006Australia Network of Aquaculture Centres in Asia-Pacific Bangkok 10900Thailand School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesJinan 250117China

Brine shrimp(Artemia)has existed on Earth for 400 million years and has major ecological importance in hypersaline *** a crucial live food in aquaculture,brine shrimp cysts have become one of the most important aquatic products traded ***,our understanding of the biodiversity,prevalence and global connectedness of viruses in brine shrimp is still very limited.A total of 143 batches of brine shrimp(belonging to seven species)cysts were collected from six continents including 21 countries and more than 100 geographic locations worldwide during 1977–*** total,55 novel RNA viruses were identified,which could be assigned to 18 different viral families and related *** viruses were dsRNA viruses,16 were+ssRNA viruses,and 28 were−ssRNA *** analyses of the RNA-directed RNA polymerase(RdRp)showed that brine shrimp viruses were often grouped with viruses isolated from other invertebrates and ***,most brine shrimp viruses were related to those from different hosts that might feed on brine shrimp or share the same ecological niche.A notable case was the novel brine shrimp noda-like virus 3,which shared 79.25%(RdRp)and 63.88%(capsid proteins)amino acid identity with covert mortality nodavirus(CMNV)that may cause losses in *** addition,both virome composition and phylogenetic analyses revealed global connectedness in certain brine shrimp viruses,particularly among Asia and Northern *** highlights the incredible species diversity of viruses in these ancient species and provides essential data for the prevalence of RNA viruses in the global aquaculture *** broadly,these findings provide novel insights into the previously unrecognized RNA virosphere in hypersaline ecosystems worldwide and demonstrate that human activity might have driven the global connectedness of brine shrimp viruses.

关键词： brine shrimp Artemia virus diversity novel virus discovery global connectedness hypersaline ecosystem

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血管内皮生长因子165/骨形态发生蛋白改善缺氧复氧状态下成骨细胞损伤

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中国组织工程研究 2024年第35期28卷 5669-5674页

作者：赵伊婷张裕祥马洁何雪娇新疆医科大学第六附属医院骨科新疆维吾尔自治区乌鲁木齐市830001 新疆医科大学第六附属医院检验科新疆维吾尔自治区乌鲁木齐市830001

背景:研究发现,血管内皮生长因子165和骨形态发生蛋白两种因子在缺氧复氧过程中相互作用,通过调节细胞内信号通路的活化,参与骨细胞损伤的修复过程。目的:进一步探究血管内皮生长因子165/骨形态发生蛋白与缺氧复氧成骨细胞损伤的关系分... 详细信息

背景:研究发现,血管内皮生长因子165和骨形态发生蛋白两种因子在缺氧复氧过程中相互作用,通过调节细胞内信号通路的活化,参与骨细胞损伤的修复过程。目的:进一步探究血管内皮生长因子165/骨形态发生蛋白与缺氧复氧成骨细胞损伤的关系分析。方法:取成骨细胞,建立缺氧复氧损伤模型,建模前后Real-Time PCR法和免疫印迹法检测血管内皮生长因子165、骨形态发生蛋白2的mRNA及蛋白表达。分别给予建模后成骨细胞不同质量浓度(10,20,40ng/mL)血管内皮生长因子165或骨形态发生蛋白2处理12,24,36,48,72 h,CCK-8法检测细胞增殖,DAPI检测细胞凋亡。结果与结论:(1)与建模前相比,建模后成骨细胞中血管内皮生长因子165、骨形态发生蛋白2的mRNA和蛋白表达量显著降低(P<0.05);(2)成骨细胞增殖率随着血管内皮生长因子165质量浓度的升高而明显升高(P<0.05);成骨细胞凋亡率随着血管内皮生长因子165质量浓度的升高而明显降低(P<0.05);(3)成骨细胞增殖率随着骨形态发生蛋白2质量浓度的升高而明显升高(P<0.05);成骨细胞凋亡率随着骨形态发生蛋白质量浓度的升高而明显降低(P<0.05);(4)结果表明,血管内皮生长因子165、骨形态发生蛋白在缺氧复氧成骨细胞损伤中低表达,给予血管内皮生长因子165、骨形态发生蛋白处理后缺氧复氧成骨细胞损伤明显降低,且呈浓度依赖性,提示血管内皮生长因子、骨形态发生蛋白对缺氧复氧成骨细胞损伤有明显保护作用。

关键词：血管内皮生长因子165 骨形态发生蛋白2 缺氧复氧成骨细胞细胞凋亡

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双相情感障碍患者血清Hepcidin,LncRNA MIAT和CRE的水平表达及与病情程度关系研究

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现代检验医学杂志 2024年第3期39卷 24-28,36页

作者：史心怡王一婷卢梅宝鸡市中心医院精神卫生科陕西宝鸡721000 宝鸡市中心医院检验科陕西宝鸡721000

目的探究双相情感障碍(bipolar disorder,BPD)患者血清铁调素(Hepcidin)、血清长链非编码RNA心肌梗死转录本(long non-coding RNA myocardial infarction-associated transcript,LncRNA MIAT)和肌酐(creatinine,CRE)表达及与病情程度的关系。方法选取2021年3月~2023年3月宝鸡市中心医院收治的150例BPD患者作为研究对象并纳入BPD组,另选取健康者150例为对照组,采用汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评价抑郁,采用杨氏躁狂量表(Young mania ratings scale,YMRS)评价躁狂程度,所有研究对象均接受血清Hepcidin,LncRNA MIAT和CRE水平的检测。对比对照组和BPD组血清Hepcidin,LncRNA MIAT和CRE水平;对比不同抑郁、躁狂程度BPD组血清Hepcidin,LncRNAMIAT和CRE水平;受试者工作特征(receiver operating characteristic,ROC)曲线分析血清Hepcidin,LncRNA MIAT和CRE对BPD的诊断价值;分析血清Hepcidin,LncRNA MIAT和CRE与BPD病情程度的关系。结果BPD组血清Hepcidin(46.17±9.17ng/L),LncRNA MIAT(11.92±2.65)和CRE(74.02±11.89μmol/L)水平均明显高于对照组(22.16±5.23 ng/L,4.11±0.95,57.16±8.61μmol/L),差异具有统计学意义(t=-27.856,-43.079,-14.066,均P<0.05);随着BPD患者抑郁、躁狂程度的增加,血清Hepcidin,LncRNA MIAT和CRE水平呈现升高的趋势(F=22.036,25.463,17.499;19.552,15.791,18.335,均P<0.05);血清Hepcidin,LncRNA MIAT和CRE联合检测(95%CI:0.866~0.949)对BPD诊断ROC曲线下面积为0.914,具有较高的特异度和敏感度,显著高于血清Hepcidin(95%CI:0.693~0.816),LncRNA MIAT(95%CI:0.696~0.819)和CRE(95%CI:0.587~0.701)单独检测(AUC=0.759,0.762,0.614,均P<0.05);血清Hepcidin,LncRNA MIAT和CRE与BPD患者抑郁严重程度呈显著正相关(r=0.784,0.771,0.826,均P<0.05);血清Hepcidin,LncRNA MIAT和CRE与BPD患者躁狂严重程度呈显著正相关(r=0.806,0.793,0.831,均P<0.05)。结论BPD患者血清Hepcidin,LncRNA MIAT和CRE水平相较正常人群更高,且与疾病严重程度呈显著正相关,联合检测对其具有较高诊断价值。

关键词：双相情感障碍铁调素长链非编码RNA 心肌梗死转录本肌酐

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个体间生物学特征和采血时间对肌钙蛋白T浓度影响的研究

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中华检验医学杂志 2024年第3期47卷 259-263页

作者：林依婷周琰周佳烨张春燕王蓓丽潘柏申郭玮上海市老年医学中心检验科上海201100 复旦大学附属中山医院检验科上海200032

目的探讨性别、年龄、采血时间及季节和 cTnT 检测值变化的相关性。方法本研究选取了 2019 年 1月1日至12月31日, 复旦大学附属中山医院就诊的 3 548 例非心血管疾病的人群。收集基础资料, 包括性别、年龄、采血时间、病史、临床诊断和 ... 详细信息

目的探讨性别、年龄、采血时间及季节和 cTnT 检测值变化的相关性。方法本研究选取了 2019 年 1月1日至12月31日, 复旦大学附属中山医院就诊的 3 548 例非心血管疾病的人群。收集基础资料, 包括性别、年龄、采血时间、病史、临床诊断和 cTnT 检测结果, 最终入组了男性1 840例, 女性1 708例, 年龄分布为65(53, 75)岁。使用 Kolmogorov-Smirnov(K-S)检验评估数据分布, 其中非正态分布的数据以M(Q1, Q3)表示。使用 Mann-WhitneyU 检验比较男性与女性之间的 cTnT 浓度, 分析性别因素对cTnT检测结果的影响, 使用 Kruskal-Wallis 检验比较各性别组之间的 cTnT 水平, 分析不同采血时间、季节等因素与 cTnT 浓度的相关性。结果在 60 岁以上的人群中, 男性和女性的cTnT 浓度均随着年龄的增长而逐渐升高, cTnT 在 90 岁以上的个体中达到最高水平(男性为 0.028 ng/ml, 女性为 0.018 ng/ml)。全年龄组分析中显示, 男性cTnT 水平(0.012 ng/ml)均高于女性(0.009 ng/ml)(H=6.340, Png/ml 和 0.012 ng/ml)。分析季节对cTnT分泌的生理性影响, 结果显示春季和冬季的cTnT 水平(0.012 ng/ml)通常高于夏、秋两季(0.010 ng/ml)。结论 cTnT 检测值与性别、年龄、采血时间和季节等因素相关。临床工作中分析cTnT检测结果时, 应综合考虑个体的性别和年龄, 以及采血时间点和季节因素的影响。

关键词：肌钙蛋白生物学因素性别年龄采血时间季节变化

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肿瘤科护士循证护理能力现状调查及影响因素分析

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中华现代护理杂志 2024年第15期30卷 2051-2055页

作者：李梦璐王安龙徐慧骆易婷浙江省肿瘤医院胸外科杭州310022

目的了解肿瘤科护士循证护理能力现状及影响因素,为提高肿瘤科护士循证护理能力提供参考。方法采用便利抽样法,于2022年9月—2023年1月选取在浙江省肿瘤医院从事护理工作的在职护士300名为研究对象,采用中文版循证实践信念-知识-实施测... 详细信息

目的了解肿瘤科护士循证护理能力现状及影响因素,为提高肿瘤科护士循证护理能力提供参考。方法采用便利抽样法,于2022年9月—2023年1月选取在浙江省肿瘤医院从事护理工作的在职护士300名为研究对象,采用中文版循证实践信念-知识-实施测评量表对其进行调查。采用单因素分析和多重线性回归分析对护士循证护理能力的影响因素进行分析。结果300名肿瘤科护士循证护理能力得分为(44.50±10.78)分。3个维度中,信念维度条目均分最高,实施维度条目均分最低。多重线性回归分析结果显示,性别、年龄、接受循证培训、参加循证项目、阅读专业文献、5年内论文发表情况、医学统计学水平是肿瘤科护士循证护理能力的影响因素(均P<0.05)。结论肿瘤科护士循证护理能力处于较低水平,护理管理者和教育者应关注女性、高龄护士群体,加大循证护理培训力度,以提升肿瘤科护士循证护理能力及整体护理队伍科研素质。

关键词：肿瘤护士循证护理能力影响因素

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紫苏精油和姜汁对烤武昌鱼的风味影响

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现代食品科技 2024年第7期40卷 193-202页

作者：黄丽琪胡传峰鲁怡婷刘煊魏凌云乔宇汪兰吴文锦石柳武汉工程大学环境生态与生物工程学院湖北武汉430205 湖北省农业科学院农产品加工与核农技术研究所湖北武汉430064 湖北工业大学生物工程与食品学院湖北武汉430064

为探究紫苏和姜汁作为去腥剂对烤武昌鱼的风味影响,该实验选用紫苏精油和姜汁腌制武昌鱼后经烤箱加工制成样品,通过电子鼻、电子舌等仪器以及对挥发性风味成分、游离氨基酸、呈味核苷酸的检测对其风味物质进行了研究分析。结果表明紫苏... 详细信息

为探究紫苏和姜汁作为去腥剂对烤武昌鱼的风味影响,该实验选用紫苏精油和姜汁腌制武昌鱼后经烤箱加工制成样品,通过电子鼻、电子舌等仪器以及对挥发性风味成分、游离氨基酸、呈味核苷酸的检测对其风味物质进行了研究分析。结果表明紫苏精油和姜汁的腌制可以使烤武昌鱼在色泽、气味和滋味上均呈现较大差异。与对照组相比,紫苏精油和姜汁可以减少腥味物质的形成,并产生掩盖腥味的物质,其中1-辛烯-3-醇含量减少为0.74 ng/g,α-松油醇、芳樟醇、莰烯、β-蒎烯等香料物质的增加,从而使得武昌鱼的风味更丰富。紫苏&姜汁组的烤武昌鱼的鲜味物质GMP的含量高达87.75 mg/g,苦味物质HxR的含量低至为4.49 mg/g,且苦味氨基酸含量远低于其他组,同时其EUC(味精当量)含量最高为224.83 g MSG/100 g。综上所述,紫苏精油和姜汁可以改善烤武昌鱼的风味和滋味。

关键词：武昌鱼紫苏精油姜汁风味滋味

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Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae

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Molecular Plant 2019年第4期12卷 474-488页

作者： Yi Xin Chen Shen yiting She Hong Chen Cong Wang Li Wei Kangsup Yoon Danxiang Han Qiang Hu Jian Xu Single-Cell Center CAS Key Laboratory of Biofuels and Shandong Laboratory of Energy Genetics Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong 266101 China Center for Microalgal Biotechnology and Biofuels Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei 430072 China Core Laboratory Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong 266101 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao Shandong 266237 China University of Chinese Academy of Sciences Beijing 100049 China

The composition of polyun saturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health ben efits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in si Iico, ex vivo, and in vivo profili ng of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respective!y. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops.

关键词： microalgae polyunsaturated fatty acids diacylglycerol acyltransferase Nannochloropsis oceanica

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Shear-responsive boundary-lubricated hydrogels attenuate osteoarthritis

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Bioactive Materials 2022年第10期7卷 472-484页

作者： yiting Lei Xingkuan Wang Junyi Liao Jieliang Shen Yuling Li zh.ngwei Cai Ning Hu Xiaoji Luo Wenguo Cui Wei Huang Department of Orthopedics The First Affiliated Hospital of Chongqing Medical UniversityOrthopedic Laboratory of Chongqing Medical UniversityNo.1 Youyi RoadYuzhong DistrictChongqing400016PR China Department of Orthopaedics Affiliated Hospital of North Sichuan Medical CollegeNo.1 the South of Maoyuan RoadNanchongSichuan637000PR China Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai200025PR China

Lipid-based boundary layers formed on liposome-containing hydrogels can facilitate ***,these boundary layers can be damaged by shear,resulting in decreased ***,a shear-responsive boundary-lubricated drug-loaded hydrogel is created by incorporating celecoxib(CLX)-loaded liposomes within dynamic covalent bond-based hyaluronic acid(HA)hydrogels(CLX@Lipo@HA-gel).The dynamic cross-linked network enables the hydrogel to get restructured in response to shear,and the HA matrix allows the accumulation of internal liposome microreservoirs on the sliding surfaces,which results in the formation of boundary layers to provide stable ***,hydration shells formed surrounding the hydrogel can retard the degradation process,thus helping in sustaining ***,in vitro and in vivo experiments found that CLX@Lipo@HA-gels can maintain anabolic-catabolic balance,alleviate cartilage wear,and attenuate osteoarthritis progression by delivering CLX and shear-responsive boundary ***,CLX@Lipo@HA-gels can serve as shear-responsive boundary lubricants and drug-delivery vehicles to alleviate friction-related diseases like osteoarthritis.

关键词： Boundary lubrication Injectable hydrogels Shear-responsive Liposomes Osteoarthritis

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NLRP3 Inflammasome in Relation to Glucose and Lipid Metabolism, and Insulin Resistance in Diabetes and Pre-Diabetes

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Yangtze Medicine 2023年第1期7卷 1-10页

作者： Shujuan Hu Dang Liu Yinqi zh.ng yiting Ding Bingqing Li Xianwang Wang School of Education and Physical Education Yangtze University Jingzhou China School of Physical Education and Science Jishou University Jishou China Department of Biochemistry and Molecular Biology Center for Molecular Medicine Health Science Center Yangtze University Jingzhou China Clinical Laboratory The Second School of Clinical Medicine and Jingzhou Central Hospital Yangtze University Jingzhou China

Aims: To investigate the relationship among NLRP3 inflammasome, glucose and lipid metabolism, and insulin resistance (IR) in the serum of patients with diabetes and pre-diabetes. Methods: A total of 100 patients with abnormal blood glucose divided into the pre-diabetes mellitus (PDM) group (N = 46) and the type 2 diabetes mellitus (T2DM) group (N = 54). 20 normoglycemic subjects (ng, N = 20) were selected as a control group. The serum levels of glucose and lipid metabolism, IR, and the expression of NLRP3, ASC and Caspase-1 were measured. Besides, the correlations of NLRP3 inflammasome with glucose and lipid metabolism, and IR were analyzed. Results: Compared with the ng group, the levels of NLRP3, ASC, Caspase-1, FBG, HbA1C, TG, LDL-C, FINs, and HOMA-IR were higher (P β were lower (P P β were seen (P P β. Regression analysis further showed that blood glucose related indexes, FINs, and NLRP3 have made a decisive contribution to IR. Conclusions: Collectively, this evidence suggested that NLRP3 is closely related to glucose and lipid metabolism, and IR, and activated in PDM and T2DM.

关键词： Type 2 Diabetes Mellitus Pre-Diabetes Mellitus NLRP3 Glucose and Lipid Metabolism Insulin Resistance

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Research on the Spillover Effects of Digital Transformation on the Sustainable Growth of Green Schools

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Proceedings of Business and Economic Studies 2023年第6期6卷 16-23页

作者： yiting Qiu Qiuhua Chen Poh Soon Joseph ng zhejiang Technical Institute of Economics Hangzhou 3018Zhejiang ProvinceChina Innovation and Technology Program Engineering and Quantity SurveyingINTI International UniversityNilai 71800Negeri SembilanMalaysia Faculty of Data Science and Information Technology INTI International UniversityNilai 71800Negeri SembilanMalaysia

The development of society and the advancement of science and technology have led to the widespread integration of digital transformation in the field of ***,the current establishment of green schools faces various challenges,including non-environmental building facilities,high renovation costs,low organizational management efficiency,high energy consumption,outdated office tools,and insufficient environmental awareness among teachers and *** thorough research and analysis,it becomes evident that digital technology can play a pivotal role in addressing these challenges and contribute to all aspects of green school *** incorporation of digital thinking concepts is essential for the construction of ecologically civilized campuses and inclusive *** process of digital design and transformation proves instrumental in optimizing both software and hardware facilities within the campus,thereby reducing energy ***,comprehensive digital teaching management enhances overall efficiency in management and service *** digital teaching and learning models emerge as transformative tools,providing new avenues to create low-carbon,green classrooms for both teachers and *** exploring the application of digital transformation in establishing green schools and examining the resulting spillover effects,valuable insights can be *** insights,in turn,serve as reference points for building diversified digital technology paths on campus and fostering the creation of green schools.

关键词： Digital transformation Green schools Spillover effect

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