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Nitrogen rhizodeposition from corn and soybean,and its contribution to the subsequent wheat crops

Journal of Integrative Agriculture 2024年第7期23卷 2446-2457页

作者： Sainan Geng Lantao Li Yuhong Miao yinjie zhang Xiaona Yu Duo zhang Qirui Yang Xiao zhang Yilun Wang College of Resources and Environment Henan Agricultural UniversityZhengzhou 450002China

Nitrogen(N)is a key factor in the positive response of cereal crops that follow leguminous crops when compared to gramineous crops in rotations,with the nonrecyclable rhizosphere-derived N playing an important ***,quantitative assessments of differences in the N derived from rhizodeposition(NdfR)between legumes and gramineous crops are lacking,and comparative studies on their contributions to the subsequent cereals are *** this study,we conducted a meta-analysis of NdfR from leguminous and gramineous crops based on 34 observations published *** addition,pot experiments were conducted to study the differences in the NdfR amounts,distributions and subsequent effects of two major wheat(Triticum aestivum L.)-preceding crops,corn(Zea mays L.)and soybean(Glycine max L.),by the cotton wick-labelling method in the main wheat-producing areas of *** meta-analysis results showed that the NdfR of legumes was significantly greater by 138.93%compared to gramineous *** our pot experiment,the NdfR values from corn and soybean were 502.32 and 944.12 mg/pot,respectively,and soybean was also significantly higher than corn,accounting for 76.91 and 84.15%of the total belowground nitrogen of the plants,***,in different soil particle sizes,NdfR was mainly enriched in the large macro-aggregates(>2 mm),followed by the small macro-aggregates(2–0.25 mm).The amount and proportion of NdfR in the macro-aggregates(>0.25 mm)of soybean were 3.48 and 1.66 times higher than those of corn,respectively,indicating the high utilization potential of soybean *** the N accumulation of subsequent wheat,the contribution of soybean NdfR to wheat was approximately 3 times that of corn,accounting for 8.37 and 4.04%of the total N uptake of wheat,*** conclusion,soybean NdfR is superior to corn in terms of the quantity and distribution ratio of soil *** future field production,legume NdfR should be included in the nitrogen p

关键词： crop rotation nitrogen rhizodeposition meta-analysis soil aggregates nitrogen transfer

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Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils

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Journal of Integrative Agriculture 2024年第6期23卷 2083-2098页

作者： Shuo Yuan Ruonan Li yinjie zhang Hao'an Luan Jiwei Tang Liying Wang Hongjie Ji Shaowen Huang State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Agricultural Resources and Environment Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051China College of Resources and Environment Henan Agricultural UniversityZhengzhou 450002China College of Forestry Hebei Agricultural UniversityBaoding 071000China

Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management *** 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of different partial substitution treatments on crop yields and the transformation of nitrogen fractions in greenhouse vegetable *** treatments with equal N,P_(2)O_(5),and K_(2)O inputs were selected,including complete inorganic fertilizer N(CN),50%inorganic fertilizer N plus 50%pig manure N(CPN),50%inorganic fertilizer N plus 25%pig manure N and 25%corn straw N(CPSN),and 50%inorganic fertilizer N plus 50%corn straw N(CSN).Organic substitution treatments tended to increase crop yields since the 6th cropping period compared to the CN *** the 8th to the 22nd cropping periods,the highest yields were observed in the CPSN treatment where yields were 7.5-11.1%greater than in CN *** 11-year fertilization,compared to CN,organic substitution treatments significantly increased the concentrations of NO_(3)^(-)-N,NH_(4)^(+)-N,acid hydrolysis ammonium-N(AHAN),amino acid-N(AAN),amino sugar-N(ASN),and acid hydrolysis unknown-N(AHUN)in soil by 45.0-69.4,32.8-58.1,49.3-66.6,62.0-69.5,34.5-100.3,and 109.2-172.9%,*** analysis indicated that soil C/N and OC concentration significantly affected the distribution of N *** highest concentrations of NO_(3)^(-)-N,AHAN,AAN,AHUN were found in the CPSN *** substitution treatments increased the activities ofβ-glucosidase,β-cellobiosidase,N-acetyl-glucosamidase,L-aminopeptidase,and phosphatase in the *** substitution treatments reduced vector length and increased vector angle,indicating alleviation of constraints of C and N on soil *** substitution treatments increased the total concentrations of phospholipid fatty acids(PLFAs)in the soil by 109.9-205.3%,and increased the relative abundance of G^(+)bacteria and fungi taxa,but decreased the relat

关键词： organic substitution management greenhouse vegetable production N fractions microbial community composition extracellular enzyme activity enzyme stoichiometry coefficients

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miR-200b-3p accelerates progression of pituitary adenomas by negatively regulating expression of RECK

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Oncology Research 2024年第5期32卷 933-941页

作者： XIAOXI WANG YANFEI JIA QIANG LI QIANG YANG YINGFENG LIU BEIFENG WEI XIANG NIU yinjie zhang XIAODONG LUO ZIYU ZHAO PENG WANG Department of Neurosurgery Tianshui First People’s HospitalTianshuiChina Department of Neurosurgery The Second Affiliated Hospital of Lanzhou UniversityLanzhouChina

MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is *** study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for *** was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and *** well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene *** expression of miR-200b-3p in clinical samples was analyzed by in situ *** of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by ***3 cell viability and proliferation were detected using CCK8 and EdU *** was detected by flow cytometry and western *** healing and Transwell assays were used to detect cell migration and *** effects of miR-200b-3p and RECK on GH3 cells were verified using salvage ***-200b-3p was highly expressed in pituitary tumor *** of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix ***,miR-200b-3p negatively regulated *** expression of RECK in pituitary adenoma tissues was lower than that in neighboring ***-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK *** summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of *** findings provide a new target for the treatment of pituitary adenoma.

关键词： Pituitary adenomas miR-200b-3p RECK Matrix metalloproteinase

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施钾对夏花生产量、品质及光温生理特性的影响

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中国农业科学 2024年第7期57卷 1335-1349页

作者：杨启睿李岚涛张潇张倩张银杰张铎王宜伦河南农业大学资源与环境学院

【目的】探究不同施钾水平对夏花生产量、品质及生育期钾素积累动态、光温生理特性和根系形态的影响，为花生合理施钾提供科学依据。【方法】2021—2022年在河南省温县进行钾肥用量田间试验，供试品种为豫花22，设施钾量0(K0)、45 kg·h... 详细信息

【目的】探究不同施钾水平对夏花生产量、品质及生育期钾素积累动态、光温生理特性和根系形态的影响，为花生合理施钾提供科学依据。【方法】2021—2022年在河南省温县进行钾肥用量田间试验，供试品种为豫花22，设施钾量0(K0)、45 kg·hm-2(K45)、90 kg·hm-2(K90)、135 kg·hm-2(K135)和180 kg·hm-2(K180)5个处理，于成熟期测定夏花生荚果产量和品质，并分别于苗期、花针期、结荚期、饱果期测定叶片SPAD值、冠层光合有效辐射和冠层温度，分析植株钾积累量和根系形态。【结果】随施钾量增加，两年度花生荚果产量可分别用“线性+平台”和“一元二次方程”拟合，适宜施钾量分别为164和135 kg·hm-2，施钾处理平均增产17%。成熟期籽粒粗蛋白、含油量和氨基酸含量均随施钾量增加呈先升高后趋于稳定趋势。与不施钾相比，施钾处理籽粒粗蛋白、含油量和氨基酸含量两年度平均增幅分别为7.85%、3.98%和13.97%，效果显著。运用Logistic方程对夏花生钾素积累量进行非线性回归拟合，得出施钾主要提高了花生钾素最大积累速率(Vmax)和平均积累速率(Vmean)，推迟吸收峰值的出现(Tmax)，延长快速积累期(Δt)与活跃积累期(Taas)，促进夏花生持续快速生长发育。此外，各生育时期冠层最高温、最低温与平均温度均随施钾量的增加显著降低；施钾135 kg·hm-2可显著增加花生叶片SPAD值与冠层光合有效辐射量(APAR)和分量(FPAR)，并对根系形态具有积极影响。【结论】合理施钾可显著提高夏花生产量、改善品质、促进钾素积累利用并显著改善生育期光温生理性能。本试验条件下夏花生推荐施钾量为135—160 kg·hm-2。

关键词：夏花生施钾量产量品质冠层温度

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针刺疗法联合中医定向透药治疗腰椎间盘突出症临床研究

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河南中医 2024年第3期44卷 427-432页

作者：李海涛张殷杰陈辉江树上海市崇明区城桥镇社区卫生服务中心上海202150 上海交通大学医学院附属新华医院崇明分院上海202150 高安市人民医院江西高安330899

目的:观察针刺疗法联合中医定向透药治疗腰椎间盘突出症(lumbar disc herniation,LDH)的临床疗效。方法:选择上海市崇明区城桥镇社区卫生服务中心中医科2020年8月至2021年12月门诊就诊的LDH患者90例,按照随机数字表法分为针刺药透组、... 详细信息

目的:观察针刺疗法联合中医定向透药治疗腰椎间盘突出症(lumbar disc herniation,LDH)的临床疗效。方法:选择上海市崇明区城桥镇社区卫生服务中心中医科2020年8月至2021年12月门诊就诊的LDH患者90例,按照随机数字表法分为针刺药透组、单纯针刺组、单纯药透组,每组各30例。单纯针刺组给予针刺疗法治疗,单纯药透组给予中医定向透药治疗,针刺药透组给予针刺疗法联合中医定向透药治疗。观察3组患者治疗前后疼痛视觉模拟评分(visual analogue score,VAS)、腰椎功能障碍指数量表(the oswestry disability index,ODI)评分、日本骨科学会下腰痛量表(Japanese orthopaedic association waist disorders,JOA)评分、血清代谢物(β-隐黄素、柠檬酸、丙酮酸、赖氨酸)变化情况及临床疗效、不良反应发生情况。结果:针刺药透组治疗2周后、治疗4周后、治疗后2周VAS评分、ODI评分均低于单纯针刺组、单纯药透组,差异均有统计学意义(P<0.05)。针刺药透组治疗2周后、治疗4周后、治疗后2周JOA评分高于单纯针刺组、单纯药透组,差异有统计学意义(P<0.05)。单纯针刺组有效率为73.33%,单纯药透组有效率为76.67%,针刺药透组有效率为96.67%,针刺药透组有效率高于单纯针刺组、单纯药透组,差异有统计学意义(P<0.05)。针刺药透组治疗后β-隐黄素、柠檬酸、丙酮酸、赖氨酸水平均高于单纯针刺组、单纯药透组,差异有统计学意义(P<0.05)。结论:针刺疗法联合中医定向透药治疗LDH,可减轻患者疼痛程度,改善腰椎功能。

关键词：腰椎间盘突出症针刺疗法中医定向透药

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大剂量维生素B6对大鼠严重创伤后应激性肝细胞死亡方式的影响及可能机制

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中华创伤杂志 2024年第6期40卷 558-568页

作者：张寅杰王之怀唐雪琳周海洋高鹏朱春富贾中芝岳茂兴秦锡虎南京医科大学研究生院南京210000 南京医科大学附属常州第二人民医院肝胆外科常州213000 南京医科大学附属常州第二人民医院ICU 常州213000 南京医科大学附属常州第二人民医院创伤中心常州213000 南京医科大学附属常州第二人民医院介入血管科常州213000

目的探讨大剂量维生素B6对大鼠严重创伤后应激性肝细胞死亡方式的影响及可能机制。方法选取32只雄性SD大鼠,按随机数字表法分为假手术组、假手术+B6组、创伤组、创伤+B6组,每组8只。采用腹壁创伤、双侧股骨骨折、单侧颅脑损伤和股动脉抽... 详细信息

目的探讨大剂量维生素B6对大鼠严重创伤后应激性肝细胞死亡方式的影响及可能机制。方法选取32只雄性SD大鼠,按随机数字表法分为假手术组、假手术+B6组、创伤组、创伤+B6组,每组8只。采用腹壁创伤、双侧股骨骨折、单侧颅脑损伤和股动脉抽血4 ml的方法构建大鼠严重创伤模型。假手术+B6组、创伤+B6组予生理盐水+大剂量维生素B6治疗;假手术组、创伤组仅滴注生理盐水治疗。在伤后36 h收集大鼠肝脏组织:(1)二代测序筛选创伤组与创伤+B6组大鼠肝组织的差异基因,并分析可能参与的细胞死亡方式。(2)验证大剂量维生素B6能否影响假手术组、假手术+B6组、创伤组、创伤+B6组大鼠肝细胞的各种细胞死亡方式,包括通过脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)染色验证凋亡;通过混合谱系激酶结构域样蛋白(MLKL)免疫组化染色验证坏死性凋亡;通过透射电镜验证自噬;通过检测各组大鼠肝组织内丙二醛(MDA)与氧化型谷胱甘肽水平、二氨基联苯氨(DAB)加强法普鲁士蓝染色、透射电镜、酰基辅酶A合成酶长链家族成员4(ACSL4)免疫组化染色验证铁死亡。(3)生物信息学分析[基因本体(GO)、京都基因与基因组百科全书(KEGG)、基因富集分析(GSEA)]创伤组与创伤+B6组大鼠肝组织测序结果代表的生物学过程及信号通路。结果(1)创伤组与创伤+B6组大鼠肝组织存在显著差异表达的基因有344个(上调137个,下调207个),涉及与凋亡、自噬、坏死性凋亡、铁死亡和焦亡相关的18个基因。(2)假手术组、假手术+B6组、创伤组、创伤+B6组TUNEL染色未体现明显凋亡差异;创伤后肝组织MLKL蛋白表达量增加,其中创伤+B6组MLKL蛋白表达量低于创伤组;电镜下可见创伤后大鼠肝细胞的自噬活动显著增加,其中创伤+B6组的细胞自噬活动低于创伤组;假手术组、假手术+B6组、创伤组、创伤+B6组大鼠肝组织内MDA水平分别为(0.20±0.05)nmol/mg、(0.17±0.07)nmol/mg、(0.69±0.11)nmol/mg、(0.52±0.07)nmol/mg(P<0.01),其中创伤组水平最高,创伤+B6组较创伤组降低;上述四组大鼠肝组织内氧化型谷胱甘肽水平分别为(11.75±2.09)μmol/g、(11.69±1.66)μmol/g、(19.75±3.40)μmol/g、(14.51±1.46)μmol/g(P<0.01),其中创伤组水平最高,创伤+B6组较创伤组降低;创伤后肝组织内铁沉积显著增加,其中创伤+B6组的肝组织内铁沉积低于创伤组;电镜下创伤+B6组线粒体膜密度显著低于创伤组;创伤+B6组ACSL4蛋白表达量低于创伤组。(3)GO、KEGG、GSEA富集分析提示大剂量维生素B6可能通过增强肝细胞胆固醇合成代谢、减轻氧化应激,实现减轻创伤后肝细胞损伤,恢复肝细胞正常功能。结论大剂量维生素B6通过作用于坏死性凋亡、自噬、铁死亡来减轻大鼠严重创伤后应激性肝损伤,其分子机制可能与增强肝细胞胆固醇合成代谢、减轻氧化应激有关。

关键词：维生素B6 应激障碍创伤性肝功能不全细胞死亡

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Functional annotation from the genome sequence of the giant panda

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Protein & Cell 2012年第8期3卷 602-608页

作者： Tong Huo yinjie zhang Jianping Lin College of Life Sciences Nankai UniversityTianjin 300071China College of Pharmacy Nankai UniversityTianjin 300071China State Key Laboratory of Medicinal Chemical Biology Nankai UniversityTianjin 300071China

The giant panda is one of the most critically endangered species due to the fragmentation and loss of its *** the functions of proteins in this animal,especially specific trait-related proteins,is therefore necessary to protect the *** this work,the functions of these proteins were investigated using the genome sequence of the giant *** on 21,001 proteins and their functions were stored in the Giant Panda Protein Database,in which the proteins were divided into two groups:20,179 proteins whose functions can be predicted by GeneScan formed the known-function group,whereas 822 proteins whose functions cannot be predicted by GeneScan comprised the unknown-function *** the known-function group,we further classified the proteins by molecular function,biological process,cellular component,and tissue *** the unknown-function group,we developed a strategy in which the proteins were filtered by cross-Blast to identify panda-specific proteins under the assumption that proteins related to the panda-specific traits in the unknown-function group *** this filtering procedure,we identified 32 proteins(2 of which are membrane proteins)specific to the giant panda genome as compared against the dog and horse *** on their amino acid sequences,these 32 proteins were further analyzed by functional classification using SVM-Prot,motif prediction using MyHits,and interacting protein prediction using the Database of Interacting *** proteins were predicted to be zinc-binding proteins,thus affecting the activities of nucleic *** 32 panda-specific proteins will be further investigated by structural and functional analysis.

关键词： Giant panda GPPD cross-Blast

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The evaluation of voting rules based on voting networks:Weber problem with support domain

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Journal of Control and Decision 2021年第4期8卷 444-452页

作者： Chunxiang Guo yinjie zhang Ruili Shi Dongzhi Wang Business School Sichuan UniversityChengduPeople’s Republic of China Department of Business Management Chongqing Business Vocational CollegeChongqingChongqingPeople’s Republic of China School of Computer Southwest University of Science and TechnologyMianyangPeople’s Republic of China

This study focuses on improving the Weber model to obtain a more optimised solution, by whichwe can measure effectively the quality of the voting rules. By introducing the voter’s supportingdomain and the candidate’s supported degree to depict the emotional factors of voters and thereal voting network, we propose the concept of validity for a candidate. Upon the traditionalWeber model, two improved models are presented and the corresponding global optimal candidateis used as the evaluation benchmark for the voting rules. The experiments show that theoptimal solution of the two models has better robustness in complex voting networks, can beused as a standard to evaluate the voting rules. When the support degree of voter is the mainfactor, the Condorcet rule is optimal in most cases, and when the validity of the candidate is takenas the main factor, the Approval rule must be the best.

关键词： Weber networks network

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New nsp8 isoform suggests mechanism for tuning viral RNA synthesis

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Protein & Cell 2010年第2期1卷 198-204页

作者： Shuang Li Qi Zhao yinjie zhang Yang zhang Mark Bartlam Xuemei Li Zihe Rao National Laboratory of Macromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Structural Biology Laboratory Tsinghua UniversityBeijing 100084China Tianjin Key Laboratory of Protein Science College of Life SciencesNankai UniversityTianjin 300071China

During severe acute respiratory syndrome coronavirus(SARS-CoV)infection,the activity of the replication/transcription complexes(RTC)quickly peaks at 6 hours post infection(h.p.i)and then diminishes significantly in the late post-infection ***“down-up-down”regulation of RNA synthesis distinguishes different viral stages:primary translation,genome replication,and finally viron *** the nsp8 as the primase in RNA synthesis,we confirmed that the proteolysis product of the primase(nsp8)contains the globular domain(nsp8C),and indentified the resectioning site that is notably conserved in all the three groups of *** subsequently crystallized the complex of SARS-CoV nsp8C and nsp7,and the 3-D structure of this domain revealed its capability to interfuse into the hexadecamer *** specific proteolysis may indicate one possible mechanism by which coronaviruses to switch from viral infection to genome replication and viral assembly stages.

关键词： nsp8 SARS-CoV RNA primase viral life cycle

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Combination of liver, hippocampus and serum metabolomics revealed therapeutical mechanism of Ziziphi Spinosae Semen on P-chlorophenylalanine induced insomnia rats

Combination of liver, hippocampus and serum metabolomics rev...

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中国睡眠研究会第十五届全国学术年会

作者： Yingxin Chu yinjie zhang Jiaxing Liu Chenhui Du Yan Yan Modern Research Center for Traditional Chinese Medicine Shanxi University School of Traditional Chinese Materia Medica Shanxi University of Chinese Medicine Shanxi Dayi Hospital affiliated to Shanxi Medical University

Purpose: Insomnia was a common sleep disorder with a current estimated global prevalence rate of ～10-20%.Many studies had suggested the etiology of insomnia may be related to many factors such as genetics, neuroendocrine, and *** sedative-hypnotics, such as benzodiazepine hypnotics, exhibited certain undesirable side effects, including tolerance, lethargy, and *** contrast, traditional Chinese medicines gained increasing attention in the treatment of insomnia due to their relative *** Spinosae Semen(ZSS), the dried and ripe seed of Ziziphus jujube ***(Bunge) Hu ex ***, had been used as a sedative for treating insomnia through nourishing the heart and liver, calming the mind and nerves, and condensing sweat and producing fluid, which had been verified both clinically and ***, the explanation of ZSS underlying mechanisms remained *** purpose of the study was to illustrate the abnormality of metabolic characteristics of liver, hippocampus, and serum of P-chlorophenylalanine(PCPA) induced insomnia rats and to demonstrate the anti-insomnia mechanisms of ***: Firstly, a classic insomnia rat model, the PCPA-induced insomnia model, was ***, hematoxylin-eosin(HE) staining was employed to observe the histopathological alterations in liver and brain tissues of *** levels of 5-HT, GABA, Glu, and DA in the hippocampus were quantified using an ELISA kit, while PGD2 concentration in serum was also ***, taking the targeted organ of insomnia, liver and hippocampus, as the objects, a combination metabolomics based on UPLC-Q-TOF-MS was *** with VIP values(> 1.0) and statistical significance(P 0.1 and P < 0.05 was filtered out as the most important metabolic pathway associated with insomnia and ***: We found that ZSS could reduce the extent of brain damage, restore the

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