Combination of liver, hippocampus and serum metabolomics revealed therapeutical mechanism of Ziziphi Spinosae Semen on P-chlorophenylalanine induced insomnia rats
作者单位：Modern Research Center for Traditional Chinese Medicine Shanxi University School of Traditional Chinese Materia Medica Shanxi University of Chinese Medicine Shanxi Dayi Hospital affiliated to Shanxi Medical University
会议名称：《中国睡眠研究会第十五届全国学术年会》
会议日期：2023年
学科分类：100506[医学-中医内科学] 1006[医学-中西医结合] 1005[医学-中医学] 10[医学] 100602[医学-中西医结合临床]
摘 要：Purpose: Insomnia was a common sleep disorder with a current estimated global prevalence rate of ～10-20%.Many studies had suggested the etiology of insomnia may be related to many factors such as genetics, neuroendocrine, and *** sedative-hypnotics, such as benzodiazepine hypnotics, exhibited certain undesirable side effects, including tolerance, lethargy, and *** contrast, traditional Chinese medicines gained increasing attention in the treatment of insomnia due to their relative *** Spinosae Semen(ZSS), the dried and ripe seed of Ziziphus jujube ***(Bunge) Hu ex ***, had been used as a sedative for treating insomnia through nourishing the heart and liver, calming the mind and nerves, and condensing sweat and producing fluid, which had been verified both clinically and ***, the explanation of ZSS underlying mechanisms remained *** purpose of the study was to illustrate the abnormality of metabolic characteristics of liver, hippocampus, and serum of P-chlorophenylalanine(PCPA) induced insomnia rats and to demonstrate the anti-insomnia mechanisms of ***: Firstly, a classic insomnia rat model, the PCPA-induced insomnia model, was ***, hematoxylin-eosin(HE) staining was employed to observe the histopathological alterations in liver and brain tissues of *** levels of 5-HT, GABA, Glu, and DA in the hippocampus were quantified using an ELISA kit, while PGD2 concentration in serum was also ***, taking the targeted organ of insomnia, liver and hippocampus, as the objects, a combination metabolomics based on UPLC-Q-TOF-MS was *** with VIP values( 1.0) and statistical significance(P 0.1 and P 0.05 was filtered out as the most important metabolic pathway associated with insomnia and ***: We found that ZSS could reduce the extent of brain damage, restore the