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Regulating the expression of bradyzoite specific genes in Toxoplasma gondii by AP2 transcription factors

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Regulating the expression of bradyzoite specific genes in To...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jingwen Zhang Lihong Zhang yanqin zhou Junlong Zhao Bang Shen State key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University

A uniqueaspect of Toxoplasma gondii biology is its ability to interconvert between fast replicating tachyzoites and slowly growing bradyzoites,which are responsible for the acute and chronic stages of Toxoplasma infection *** interconversion is key for the transmission and pathogenesis of the *** very little in known about the underlying mechanisms that control this *** this study,we first purified nuclear proteins from tachyzoites and alkaline-induced *** proteomic comparisons identified a number of nuclear proteins that showed abundance difference between those two stages Subsequently we focused on a previously uncharacterized AP2 family transcription factor TgAP2 XII-5,whose transcription was increased at the bradyzoite ***2 XII-5 disruption had no discernible effect on tachyzoite replication but did resulted in a significant growth defect of *** addition,loss of AP2 XII-5 also resulted in a modest attenuation of parasite virulence,which was restored by ***-PCR and RNA-seq analyses indicated that AP2 XII-5 was involved in the regulation of gene expression in *** the bradyzoite specific marker LDH2 as a model,we showed that recombinant the AP2 domain of TgAP2 XII-5 was able to specifically bind to the promoter region of *** together,these results illuminated the roles of TgAP2 XII-5 in regulating bradyzoite gene expression and parasite virulence.

关键词： Toxoplasma gondii gene expression differentiation AP2 virulence

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农村金融深化对农民福祉的传导机制研究

经济评论 2016年第3期199卷 86-94,107页

作者：李志平周言覃华中农业大学经济管理学院

本文旨在揭示金融深化对农民福祉的传导机制,为农村金融深化政策取向从收入向福祉转型提供支持。基于程序化事实-理论分析-实证分析的研究路线,使用四川、河南、河北、湖北、云南五省的入户调查数据,借助结构分析模型,从物质产品消费、... 详细信息

本文旨在揭示金融深化对农民福祉的传导机制,为农村金融深化政策取向从收入向福祉转型提供支持。基于程序化事实-理论分析-实证分析的研究路线,使用四川、河南、河北、湖北、云南五省的入户调查数据,借助结构分析模型,从物质产品消费、地位产品消费和公共产品消费方面设计三条相对独立的传导路径,探讨农村金融深化与农民福祉的相互关系,并试图解释农村"收入-福祉"悖论背后的原因。研究结果显示:这三条路径中,公共产品消费路径对农民福祉的影响力度大于地位产品消费路径,而后者又大于收入或者物质产品消费路径。最后提出农村金融深化政策目标从收入向福祉进行调整及其相关风险防控的政策建议。

关键词：金融深化政策农民福祉传导机制结构方程公共产品

Abnormal amylopectin accumulation is detrimental to Toxoplasma virulence and cyst development

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Abnormal amylopectin accumulation is detrimental to Toxoplas...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Congcong Lyu Xuke Yang Jichao Yang Lun Hou yanqin zhou Junlong Zhao Bang Shen State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University Key Laboratory of Preventive Medicine in Hubei Province Huazhong Agricultural University

Toxoplasma gondii is a ubiquitous pathogen infecting humans and numerous animals,leading to huge socio-economic *** unique aspect of this parasite that contributes to its transmission and pathogenesis is the ability to interconvert between fast replicating tachyzoites and nearly dormant *** bradyzoites accumulate high levels of amylopectin,which is barely seen in *** its physiological significance is not fully *** we identified a starch synthase (SS) that is key for amylopectin synthesis in *** genetic ablation of this enzyme only modestly affected tachyzoite replication and cyst morphology On the other hand,deletion of SS in the△cdpk2 mutant completely abolished its massive amylopectin accumulation and restored the cyst formation and virulence *** these results suggest that amylopectin synthesis is not required for chronic infection establishment,but its abnormal accumulation is deleterious to parasite virulence and cyst development.

关键词： Toxoplasma chronic infection cyst amylopectin starch synthase

Interactions between Toxoplasma gondii micronemal protein MIC3 and host proteins Spata3 and Dkk2

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Interactions between Toxoplasma gondii micronemal protein MI...

中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Yifan Wang Rui Fang Yuan Yuan Ming Pan Min Hu yanqin zhou Bang Shen Junlong Zhao State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University Key Laboratory of development of veterinary diagnostic products Ministry of AgricultureHuazhong Agricultural University

As an obligate intracellular protozoan,Toxoplasma gondii is a successful pathogen infecting a variety of animals and *** host infection,micronemal proteins(MICs) are released to parasite surface,where they bind host cells to mediate gliding motility and host-cell *** an adhesin,the micronemal protein MIC3 plays important roles in host cell attachment,as well as modulation of host EGFR signaling ***,the specific host proteins that interact with MIC3 are unknown and the identification of such proteins will increase our understanding of how MIC3 exerts its *** this study,we sought to identify host proteins interacting with MIC3 by yeast two-hybrid *** MIC3 as bait,we screened a library expressing mouse proteins and found five mouse proteins that showed positive interactions with *** of which,spermatogenesis-associated protein 3(Spata3) and dickkopf-related protein 2(Dkk2),were further confirmed to interact with MIC3 both in vitro and in vivo by additional protein-protein interaction *** also found that the tandem repeat EGF domains of MIC3 were critical in mediating the interactions with the identified host *** is the first study to show that MIC3 interacts with host proteins that are involved in reproduction,growth and *** results will help us to better understand the functions of adhesion-associated micronemal proteins in Toxoplasma gondii.

关键词： Toxoplasma gondii MIC3 Yeast two-hybrid Dkk2 Spata3

The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang zhou Ting Li Wei Zhang Jian Wu Hui Hong Wei Quan Xinyu Qiao Chun Cui Chenmeng Qiao Weijiang Zhao yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon

An R-Smad from Haemonchus contortus(Hc-DAF-8) mediates development via the TGF-β signalling pathway

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An R-Smad from Haemonchus contortus(Hc-DAF-8) mediates devel...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Fangfang Li Robin B.Gasser Jianan Shan Wenda Di Li He Caixian zhou Chunqun Wang yanqin zhou Rui Fang Junlong Zhao Min Hu State Key Laboratory of Agricultural Microbiology Key Laboratory for the Development of Veterinary ProductsMinistry of AgricultureCollege of Veterinary MedicineHuazhong Agricultural University Melbourne Veterinary School Faculty of Veterinary and Agricultural SciencesThe University of Melbourne

Proteins of the Smad family are essential cellular mediators of the transforming growth factor-β(TGF-β) superfamily that directly transmit incoming signals from the cell surface receptors to the *** spite of their functional importance,almost nothing is known about Smad proteins in parasitic *** the present study,we identified a homologueofDAF-8 representing the receptor-regulated Smad(R-Smad) subfamily of the free-living nematode Caenorhabditis elegans,called Hc-DAF-8,in the parasitic nematode Haemonchus *** features of Hc-DAF-8(502 amino acids),including conserved R-Smad domains,and characteristic residues in the L3 loop involved in Smad-anchoring for receptor activation(SARA),are consistent with a TGF-β type I receptor-activated *** Hc-daf-8 gene was transcribed in all developmental stages of *** studied,with a high level in fourth-stage(L4) females and the highest level in adult *** ***,this protein was localised to the platymyarian muscular cells,intestine and reproductive system of L4 and adult *** assessment showed that Hc-daf-8 was able to rescue gene function in a daf-8 deficient mutant strain of ***,leading to a resumption of normal development in this free-living worm.A specific inhibitor of human Smad3(SIS3) was shown to affect *** development in *** together,these results suggest that Hc-DAF-8,encoded by the gene Hc-daf-8,is an important cellular mediator of *** development via the TGF-βsignalling *** findings underpin future explorations of this pathway in *** and other important parasitic nematodes.

关键词： Haemonchus contortus R-Smads TGF-β signalling pathway Development Gene rescue Specific inhibitor of human Smad3(SIS3)

顶空气相色谱法测定工业废水中乙醇和异丙醇研究

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环境科学与管理 2015年第9期40卷 154-156页

作者：周燕琴江苏省常州市武进区环境监测站江苏常州213159

建立了测定工业废水中乙醇和异丙醇的顶空毛细管气相色谱法.采用顶空进样技术,分析过程不使用有机试剂,避免了对分析人员和环境的危害.乙醇和异丙醇在3.00～ 20.0mg/L浓度范围内呈良好线性关系,水样测定结果重现性较好,相对标准偏差范... 详细信息

建立了测定工业废水中乙醇和异丙醇的顶空毛细管气相色谱法.采用顶空进样技术,分析过程不使用有机试剂,避免了对分析人员和环境的危害.乙醇和异丙醇在3.00～ 20.0mg/L浓度范围内呈良好线性关系,水样测定结果重现性较好,相对标准偏差范围均小于2％,乙醇加标回收率为93.6％～104.0％,异丙醇加标回收率为94.0％～ 102.0％.本方法操作简单快速,灵敏度高,能满足工业废水中乙醇和异丙醇的监测.

关键词：工业废水乙醇和异丙醇顶空气相色谱法

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基于LabVIEW的小波去噪算法的研究

电子测试 2010年第2期21卷 46-49页

作者：朱艳芹郭鑫惠州经济职业技术学院广东惠州516000 郑州江宇水务工程有限公司河南郑州450002

近年来,小波理论得到了迅速的发展。小波分析是目前国际公认的信号信息获取与处理领域的高新技术,是多学科关注的热点,是信号处理的前沿课题。由于小波变换具有低熵性、多分辨性、去相关性、选基灵活性等特性,使它成为在信号去噪领域内... 详细信息

近年来,小波理论得到了迅速的发展。小波分析是目前国际公认的信号信息获取与处理领域的高新技术,是多学科关注的热点,是信号处理的前沿课题。由于小波变换具有低熵性、多分辨性、去相关性、选基灵活性等特性,使它成为在信号去噪领域内的有力工具。本文应用小波变换算法,并将其结合LabVIEW虚拟仪器开发平台、MATLAB中小波去噪工具箱的小波去噪函数进行仿真,用模拟信号验证了其去噪算法的有效性。

关键词：小波去噪 LabVIEW MATLAB

Identification and characterization of a novel 34 kDa merozoite protein in Babesia orientalis

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Identification and characterization of a novel 34 kDa merozo...

中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Lan He Lizhe Fan Qin Liu Jinfang Hu Xiaoyan Miao Yuan Huang Pei He Junwei He Long Yu Muhammad Kasib Khan yanqin zhou Junlong Zhao State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University Key Laboratory of Development of Veterinary Diagnostic Products Ministry of AgricultureHuazhong Agricultural University National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention

A novel gene encoding a 34 kDa protein of Babesia orientalis(designated BoP34) was obtained by immunoscreening of a cDNA expression library with *** infected water buffalo *** complete nucleotide sequence of the BoP34 was 1088 bp,which contained one open reading frame(ORF),two untranslated regions(UTRs) and a poly(A) *** length of ORF was 933 bp,encoding a polypeptide of 310 aa with a predicted size of 34 *** analysis showed that the nucleotide sequence of BoP34 had 71%similarity with the sequence of Babesia bovis nuclear movement family protein(XM01611335).This suggested that BoP34 is a homologous of the movement family *** structure analysis of BoP34 amino acid sequence showed a CS domain which may interact with the ATPase domain of the heat shock protein *** sequence of BoP34 was truncated and cloned into the expression vector pET-32 a,and subsequently expressed in *** Rosetta(DE3) pLysS stain as a Trx-fusion protein(designated rBoP34-T).Antibodies in the serum of a ***-intected water buffalo specifically recognized this protein in immunoblot *** rabbit antibodies raised against the rBoP34-T detected native BoP34(34kDa) in ***-infected water buffalo *** results suggested that BoP34 might be a potential antigen for the specific detection of *** antibody in *** research is required to explore the biological function of this molecule and to establish ELISA for the diagnosis of *** in cattle.

关键词： Babesia orientalis nuclear movement protein polyclonal antibody