Interactions between Toxoplasma gondii micronemal protein MIC3 and host proteins Spata3 and Dkk2
作者单位：State Key Laboratory of Agricultural MicrobiologyCollege of Veterinary MedicineHuazhong Agricultural University Key Laboratory of development of veterinary diagnostic productsMinistry of AgricultureHuazhong Agricultural University
会议名称：《中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会》
会议日期：2015年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：Toxoplasma gondii MIC3 Yeast two-hybrid Dkk2 Spata3
摘 要：As an obligate intracellular protozoan,Toxoplasma gondii is a successful pathogen infecting a variety of animals and *** host infection,micronemal proteins(MICs) are released to parasite surface,where they bind host cells to mediate gliding motility and host-cell *** an adhesin,the micronemal protein MIC3 plays important roles in host cell attachment,as well as modulation of host EGFR signaling ***,the specific host proteins that interact with MIC3 are unknown and the identification of such proteins will increase our understanding of how MIC3 exerts its *** this study,we sought to identify host proteins interacting with MIC3 by yeast two-hybrid *** MIC3 as bait,we screened a library expressing mouse proteins and found five mouse proteins that showed positive interactions with *** of which,spermatogenesis-associated protein 3(Spata3) and dickkopf-related protein 2(Dkk2),were further confirmed to interact with MIC3 both in vitro and in vivo by additional protein-protein interaction *** also found that the tandem repeat EGF domains of MIC3 were critical in mediating the interactions with the identified host *** is the first study to show that MIC3 interacts with host proteins that are involved in reproduction,growth and *** results will help us to better understand the functions of adhesion-associated micronemal proteins in Toxoplasma gondii.