Regulating the expression of bradyzoite specific genes in Toxoplasma gondii by AP2 transcription factors
作者单位：State key Laboratory of Agricultural MicrobiologyCollege of Veterinary MedicineHuazhong Agricultural University
会议名称：《中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛》
会议日期：2019年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
关 键 词：Toxoplasma gondii gene expression differentiation AP2 virulence
摘 要：A uniqueaspect of Toxoplasma gondii biology is its ability to interconvert between fast replicating tachyzoites and slowly growing bradyzoites,which are responsible for the acute and chronic stages of Toxoplasma infection *** interconversion is key for the transmission and pathogenesis of the *** very little in known about the underlying mechanisms that control this *** this study,we first purified nuclear proteins from tachyzoites and alkaline-induced *** proteomic comparisons identified a number of nuclear proteins that showed abundance difference between those two stages Subsequently we focused on a previously uncharacterized AP2 family transcription factor TgAP2 XII-5,whose transcription was increased at the bradyzoite ***2 XII-5 disruption had no discernible effect on tachyzoite replication but did resulted in a significant growth defect of *** addition,loss of AP2 XII-5 also resulted in a modest attenuation of parasite virulence,which was restored by ***-PCR and RNA-seq analyses indicated that AP2 XII-5 was involved in the regulation of gene expression in *** the bradyzoite specific marker LDH2 as a model,we showed that recombinant the AP2 domain of TgAP2 XII-5 was able to specifically bind to the promoter region of *** together,these results illuminated the roles of TgAP2 XII-5 in regulating bradyzoite gene expression and parasite virulence.