检索结果-南通市图书馆

245 GHz subharmonic receiver with on-chip antenna for gas spectroscopy application

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Semiconductors 2018年第12期39卷 160-165页

作者： Yanfei Mao Shiju E Klaus Schmalz J.Christoph Scheytt Engineering College Zhejiang Normal University IHP Microelectronics Heinz Nixdorf Institute Paderborn University

A 2 nd transconductance subharmonic receiver for 245 GHz spectroscopy sensor applications has been proposed. The receiver consists of a 245 GHz on-chip folded dipole antenna, a CB（common base） LNA, a 2 nd transconductance SHM（subharmonic mixer）, and a 120 GHz push-push VCO with 1/64 divider. The receiver is fabricated in f;/f;= 300/500 GHz SiGe:C BiCMOS technology. The receiver dissipates a low power of 288 mW.Integrated with the on-chip antenna, the receiver is measured on-chip with a conversion gain of 15 dB, a bandwidth of 15 GHz, and the chip will be utilized in PCB board design for gas spectroscopy sensor application.

关键词： SiGe BiCMOS CB LNA 2nd transconductance subharmonic receiver 245 GHz on-chip antenna gas spectroscopy

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第7期10卷 1011-1013页

作者： Mugui Wang Yanfei Mao Yuming Lu Xiaoping Tao Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence inMolecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, The class 2/type Ⅱ clustered regularly interspaced short palindromic repeat （CRISPR）/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs （sgRNAs） to achieve multiplex gene editing in plant cells.

关键词： TARGETED MUTAGENESIS CPF1

Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2018年第8期60卷 626-631页

作者： Mugui Wang Yanfei Mao Yuming Lu Zhidan Wang Xiaoping Tao Jian-Kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.

关键词： Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems PAM Figure LEA

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第7期10卷 1007-1010页

作者： Mugui Wang Yuming Lu Jose Ramon Botella Yanfei Mao Kai Hua Jian-kang Zhu Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai 201602 China Plant Genetic Engineering Laboratory School of Agriculture and Food Sciences University of Queensland Brisbane QLD 4072 Austraria Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Dear Editor, Rice （Oryza sativa） is the staple food for more than half of the world＇s population. Technologies enabling precise and efficient DNA knock-in or replacement, hereinafter referred to as KI, have the pot... 详细信息

关键词： DNA REPLICONS GENOME

A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2015年第15期60卷 1332-1347页

作者： Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu Yanfei Mao School of Life Sciences Chongqing University Shanghai Center for Plant Stress Biology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences State Key Laboratory of Silkworm Genome Biology Southwest University Department of Horticulture and Landscape Architecture Purdue University School of Agriculture and Food Sciences University of Queensland

The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications.

关键词： RNA编辑基因组拟南芥转基因载体 DNA裂解介导程序协议描述

Common features in patients with intracerebral hemorrhage following superficial temporal artery-middle cerebral artery bypass in steno-occlusive cerebrovascular disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第20期7卷 1585-1590页

作者： Zhiqi mao Meng Li Yan Ma yanfei Chen Hongqi Zhang Feng Ling Department of Neurosurgery Xuanwu Hospital Capital Medical University Beijing 100053 China

Five patients treated for intracranial cerebral hemorrhage after superficial temporal artery-middle cerebral artery bypass in Xuwu Hospital, Capital Medical University, Beijing, China, from 2005-2011 were included in this study. Prior to superficial temporal artery-middle cerebral artery bypass, all patients showed diminished cerebrovascular reactivity and an ipsilateral ischemic lesion. Intracranial cerebral hemorrhage developed within 1-4 days following superficial temporal artery-middle cerebral artery bypass. Transcranial Doppler showed increased middle cerebral artery velocity of 50-100% in the operated hemisphere. These findings suggested that focal hyperperfusion, an ipsilateral ischemic lesion and diminished cerebrovascular reactivity are the important characteristics of intracerebral hemorrhage following superficial temporal artery-middle cerebral artery bypass in patients with steno-occlusive cerebrovascular disease.

关键词： cerebral hemorrhage STA-MCAo ,cclusion cerebrovascuiar disordersS uperfiCihy alperperfusionte mp ral artery-middlis echemic lesion cerebrovascular reactivityC erebral artery bypass stenosis

Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2023年第4期8卷 597-605页

作者： Xue Yang Zhitao mao Jianfeng Huang Ruoyu Wang Huaming Dong yanfei Zhang Hongwu Ma Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin300308China National Technology Innovation Center of Synthetic Biology Tianjin300308China School of Environmental Ecology and Biological Engineering Wuhan Institute of TechnologyWuhan430205China

Metabolic network models have become increasingly precise and accurate as the most widespread and practical digital representations of living cells.The prediction functions were significantly expanded by integrating cellular resources and abiotic constraints in recent years.However,if unreasonable modeling methods were adopted due to a lack of consideration of biological knowledge,the conflicts between stoichiometric and other constraints,such as thermodynamic feasibility and enzyme resource availability,would lead to distorted predictions.In this work,we investigated a prediction anomaly of EcoETM,a constraints-based metabolic network model,and introduced the idea of enzyme compartmentalization into the analysis process.Through rational combination of reactions,we avoid the false prediction of pathway feasibility caused by the unrealistic assumption of free intermediate metabolites.This allowed us to correct the pathway structures of L-serine and L-tryptophan.A specific analysis explains the application method of the EcoETM-like model and demonstrates its potential and value in correcting the prediction results in pathway structure by resolving the conflict between different constraints and incorporating the evolved roles of enzymes as reaction compartments.Notably,this work also reveals the trade-off between product yield and thermodynamic feasibility.Our work is of great value for the structural improvement of constraints-based models.

关键词： Genome-scale metabolic network models (GEMs) Enzymatic and thermodynamic constraints Thermodynamic driving force(MDF) Compartmentalization Multifunctional enzymes Enzyme complexes

DIW1 encoding a cladeⅠPP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2023年第8期65卷 1918-1936页

作者： Jingyi Wang Chaonan Li Long Li Lifeng Gao Ge Hu yanfei Zhang Matthew P.Reynolds Xueyong Zhang Jizeng Jia Xinguo mao Ruilian Jing National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China International Maize and Wheat Improvement Center Texcoco 56237Mexico

Drought seriously impacts wheat production(Triticum aestivum L.),while the exploitation and utilization of genes for drought tolerance are insufficient.Leaf wilting is a direct reflection of drought tolerance in plants.Clade A PP2Cs are abscisic acid(ABA)co-receptors playing vital roles in the ABA signaling pathway,regulating drought response.However,the roles of other clade PP2Cs in drought tolerance,especially in wheat,remain largely unknown.Here,we identified a gain-of-function drought-induced wilting 1(DIW1)gene from the wheat Aikang 58 mutant library by map-based cloning,which encodes a cladeⅠprotein phosphatase 2C(TaPP2C158)with enhanced protein phosphatase activity.Phenotypic analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought resistance.We found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it,thus inactivating the TaSnRK1.1–Ta AREB3 pathway.TaPP2C158 protein phosphatase activity is negatively correlated with ABA signaling.Association analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature,and seedling survival rate under drought stress.Our data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding history.This work benefits us in understanding the molecular mechanism of wheat drought tolerance,and provides elite genetic resources and molecular markers for improving wheat drought tolerance.

关键词： association analysis drought elite genetic resources map-based clone PP2C protein phosphatase wheat wilting

Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2013年第6期6卷 2008-2011页

作者： mao, yanfei Zhang, Hui Xu, Nanfei Zhang, Botao Gou, Feng Zhu, Jian-Kang Chinese Acad Sci Shanghai Ctr Plant Stress Biol Shanghai 200032 Peoples R China Univ Chinese Acad Sci CAS Shanghai 200032 Peoples R China Purdue Univ Dept Hort & Landscape Architecture W Lafayette IN 47907 USA

Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases （ZFNs）（Carroll, 2011）, Transcription Activator-Like Effector Nucleases （TALENs）（Mahfouz et al., 2011; Li et al., 2012）, and Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）-associated （Cas） systems （Cong et al., 2013） have been successfully used for gene editing in a variety of species. These systems generate double-strand breaks （DSBs） at target loci to drive site-specific DNA sequence modifica- tions. The modifications include sequence insertion and deletion and other mutations in the host genomes via the error-prone non-homologous end joining （NHEJ） pathway or sequence correction or replacement through the error-free homologous recombination （HR） pathway （Symington and Gautier, 2011）. Here, we show that the CRISPR-Cas system can be applied to generate targeted gene mutations and gene corrections in plants, and the system can also be readily engi- neered to achieve deletion of large DNA fragments and for multiplex gene editing in plants.

关键词： CAS系统基因组工程植物基因应用 DNA双链断裂重复序列同源重组核酸内切酶