Gene editing in plants:progress and challenges

作     者：Yanfei Mao Jose Ramon Botella Yaoguang Liu Jian-Kang Zhu Yanfei Mao;Jose Ramon Botella;Yaoguang Liu;Jian-Kang Zhu

作者机构：Shanghai Center for Plant Stress BiologyCAS Center of Excellence in Molecular Plant Sciences Chinese Academy of Sciences School of Agriculture and Food Sciences University of Queensland State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of Life Sciences South China Agricultural University Department of Horticulture and Landscape Architecture Purdue University 

出 版 物：《National Science Review》 (国家科学评论(英文版))

年 卷 期：2019年第6卷第3期

页      面：421-437页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the Chinese Academy of Sciences the Youth Innovation Promotion Association 

主　　题：CRISPR Cas9 genome editing base editing gene targeting crop breeding 

摘      要：The clustered regularly interspaced short palindromic repeat(CRISPR)-associated protein 9(Cas9)genome editing system is a powerful tool for targeted gene modifications in a wide range of species,including plants. Over the last few years, this system has revolutionized the way scientists perform genetic studies and crop breeding, due to its simplicity, flexibility, consistency and high efficiency. Considerable progress has been made in optimizing CRISPR/Cas9 systems in plants, particularly for targeted gene mutagenesis. However, there are still a number of important challenges ahead, including methods for the efficient delivery of CRISPR and other editing tools to most plants, and more effective strategies for sequence knock-ins and replacements. We provide our viewpoint on the goals, potential concerns and future challenges for the development and application of plant genome editing tools.