Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants

作     者：Mao, Yanfei Zhang, Hui Xu, Nanfei Zhang, Botao Gou, Feng Zhu, Jian-Kang 

作者机构：Chinese Acad Sci Shanghai Ctr Plant Stress Biol Shanghai 200032 Peoples R China Univ Chinese Acad Sci CAS Shanghai 200032 Peoples R China Purdue Univ Dept Hort & Landscape Architecture W Lafayette IN 47907 USA 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第6期

页      面：2008-2011页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 081302[工学-建筑设计及其理论] 071007[理学-遗传学] 0813[工学-建筑学] 

基　　金：中国科学院项目 

主　　题：CAS系统 基因组工程 植物基因 应用 DNA双链断裂 重复序列 同源重组 核酸内切酶 

摘      要：Dear Editor, Recently, engineered endonucleases, such as Zinc-Finger Nucleases （ZFNs） （Carroll, 2011）, Transcription Activator-Like Effector Nucleases （TALENs） （Mahfouz et al., 2011; Li et al., 2012）, and Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）-associated （Cas） systems （Cong et al., 2013） have been successfully used for gene editing in a variety of species. These systems generate double-strand breaks （DSBs） at target loci to drive site-specific DNA sequence modifica- tions. The modifications include sequence insertion and deletion and other mutations in the host genomes via the error-prone non-homologous end joining （NHEJ） pathway or sequence correction or replacement through the error-free homologous recombination （HR） pathway （Symington and Gautier, 2011）. Here, we show that the CRISPR-Cas system can be applied to generate targeted gene mutations and gene corrections in plants, and the system can also be readily engi- neered to achieve deletion of large DNA fragments and for multiplex gene editing in plants.