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Fabrication of onion-like carbon from nanodiamond by annealing

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Science China(Technological Sciences) 2009年第12期52卷 3683-3689页

作者： Qin Zou MingZhi Wang yanguo li YuCheng Zhao liangHua Zou State Key Laboratory of Metastable Materials Science and Technology Yanshan University Qinhuangdao 066004 China

Onion-like carbon (OLC) was synthesized by annealing nanodiamond in low vacuum of 1 Pa and at annealing temperatures from 500°C to 1400°C. The high-resolution transmission electron microscope (HRTEM) images, X-ray diffraction (XRD) and Raman spectrum of the OLC showed that there was no OLC when the annealing temperature was lower than 900°C. Moreover, the fragment amorphous carbon existed on the surfaces of the nanodiamond particles. At the annealing temperature of 900°C, the OLC particles began appearing and the size of the OLC particles was smaller than 5 nm. When the annealing temperature was increased from 900°C to 1400°C, the nanodiamond was transformed into OLC gradually. At the annealing temperature of 1400°C, all the nanodiamond particles were transformed into OLC completely. The OLC exhibited similarity to the original nanodiamond particles in shape. A mechanism for the OLC synthesis by annealing was provided. The graphitization started at the surfaces of the nanodiamond particles. The formation process of the OLC includes formation of graphite fragments, connection and curvature of graphite sheets between diamond (111) planes and closure of the graphite layers.

关键词： onion-like carbon(OLC) nanodiamond annealing fabrication

A new approach to promote astaxanthin accumulation via Na_2WO_4 in Haematococcus pluvialis

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Journal of Oceanology and limnology 2019年第1期37卷 176-185页

作者： li yanguo CUI DANDan ZHUO Pinli ZHANG lin SUN Xue XU Nianjun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine Sciences Ningbo University

The optimum concentration of Na_2 WO_4 was explored in relation to the cell density and astaxanthin content in Haematococcus pluvialis. Then, the cellular morphology, nitrate reductase(NR) activity, soluble sugar and protein contents, and chlorophyll ?uorescence were measured, and the transcriptional expression of carotenogenic genes was determined by quantitative real-time PCR. The results showed that 3.0 mmol/L of Na_2 WO_4 was the optimum concentration to induce astaxanthin accumulation, with a maximum content of 49.41±0.13 pg/cell reached on the tenth day. The NR activity decreased signi?cantly and continually after Na_2 WO_4 treatment. The soluble sugar content increased gradually during the experimental period and was eventually signi?cantly higher than that in the control. The soluble protein content increased rapidly,reached a maximum in day 0.5 and day 1 and then decreased. The ef fective photochemical effciency of PSII( F v'/F m') and light saturation( E k) ?rst decreased and then tended to stabilize, and NADP +-glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene expression was correlated with photosynthesis. The transcriptional expression of ipi, psy and bkt was signi?cantly increased compared with that in the control after application of Na_2 WO_4, and the relative expression of ipi reached the highest level on the ?fth day, with a 98.03±1.92-fold increase. Our results describe a new approach to promote the ef fective accumulation of astaxanthin in H. pluvialis by NR inhibitor Na_2 WO_4.

关键词： Haematococcus pluvialis astaxanthin Na2WO4 nitrate reductase quantitative real-time PCR

eRNA-IDO:A One-stop Platform for Identification,Interactome Discovery,and Functional Annotation of Enhancer RNAs

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Genomics, Proteomics & Bioinformatics 2024年第4期22卷 169-179页

作者： Yuwei Zhang lihai Gong Ruofan Ding Wenyan Chen Hao Rong yanguo li Fawziya Shameem Korakkandan Arshad Ali Lei li Qi liao School of Public Health Health Science CenterNingbo UniversityNingbo 315211China Biomedical Big Data Center the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen 518107China School of Clinical Medicine Health Science CenterNingbo UniversityNingbo 315211China Institute of Drug Discovery Technology Ningbo UniversityNingbo 315211China

Growing evidence supports the transcription of enhancer RNAs(eRNAs)and their important roles in gene ***,their interactions with other biomolecules and their corresponding functionality remain poorly *** an attempt to facilitate mechanistic research,this study presents eRNA-IDO,the first integrative computational platform for the identification,interactome discovery,and functional annotation of human ***-IDO comprises two modules:eRNA-ID and ***,eRNA-ID can identify eRNAs from de novo assembled ***-ID includes eight kinds of enhancer makers,enabling users to customize enhancer regions flexibly and *** addition,eRNA-Anno provides cell-/tissue-specific functional annotation for both new and known eRNAs by analyzing the eRNA interactome from prebuilt or user-defined networks between eRNAs and protein-coding *** prebuilt networks include the Genotype-Tissue Expression(GTEx)-based co-expression networks in normal tissues,The Cancer Genome Atlas(TCGA)-based co-expression networks in cancer tissues,and omics-based eRNA-centric regulatory ***-IDO can facilitate research on the biogenesis and functions of *** eRNA-IDO server is freely available at http://***/eRNA_IDO/.

关键词： Enhancer RNA Identification Interactome Functional annotation Web server

Characterization of Cancer Stem Cells in Laryngeal Squamous Cell Carcinoma by Single-cell RNA Sequencing

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Genomics, Proteomics & Bioinformatics 2024年第4期22卷 119-132页

作者： yanguo li Chen lin Yidian Chu Zhengyu Wei Qi Ding Shanshan Gu Hongxia Deng Qi liao Zhisen Shen The Affiliated lihuili Hospital Ningbo UniversityNingbo 315211China School of Public Health Health Science CenterNingbo UniversityNingbo 315211China Institute of Drug Discovery Technology Ningbo UniversityNingbo 315211China The Ningbo Diagnostic Pathology Center Ningbo 315021China

Cancer stem cells(CSCs)constitute a pivotal element within the tumor microenvironment(TME),driving the initiation and progression of ***,the identification of CSCs and their underlying molecular mechanisms in laryngeal squamous cell carcinoma(LSCC)remains a formidable ***,we employed single-cell RNA sequencing of matched primary tumor tissues,paracancerous tissues,and local lymph nodes from three LSCC patients to comprehensively characterize the CSCs in *** distinct clusters of stem cells originating from epithelial populations were delineated and verified as CSCs and normal stem cells(NSCs),*** were abundant in the paracancerous tissues compared to those in the tumor *** showed high expression of stem cell marker genes such as PROM1,ALDH1A1,and SOX4,and increased the activity of tumor-related hypoxia,Wnt/β-catenin,and Notch signaling *** then explored the intricate crosstalk between CSCs and the TME cells and identified targets within the TME that related with *** also found eight marker genes of CSCs that were correlated significantly with the prognosis of LSCC ***,bioinformatics analyses showed that drugs such as erlotinib,OSI-027,and ibrutinib selectively targeted the CSC-specifically expressed *** conclusion,our results represent the first comprehensive characterization of CSC properties in LSCC at the single-cell level.

关键词： Laryngeal squamous cell carcinoma Cancer stem cell Single-cell RNA sequencing Cell-cell communication Therapeutic target.

Comprehensive landscape of resistance mechanisms for neoadjuvant therapy in esophageal squamous cell carcinoma by single-cell transcriptomics

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Signal Transduction and Targeted Therapy 2023年第9期8卷 3920-3922页

作者： Yushang Yang yanguo li Haopeng Yu Zhenyu Ding Longqi Chen Xiaoxi Zeng Shunmin He Qi liao Yi Zhao Yong Yuan Department of Thoracic Surgery West China HospitalSichuan UniversityChengduSichuanChina Zhejiang Key Laboratory of Pathophysiology Health Science CenterNingbo UniversityNingboZhejiangChina West China Biomedical Big Data Center West China HospitalSichuan UniversityChengduSichuanChina

Dear Editor,Preoperative neoadjuvant therapy combined with surgical resection is the standard treatment for locally advanced esopha-geal squamous cell carcinoma(ESCC).However,more than half of patients having a partial response to neoadjuvant therapy,which is considered as a therapy-resistant phenotype and the mechan-ism is still *** heterogeneity of the ESCC with surgery alone therapy were characterized by single-cell RNA sequencing(scRNA-seq)previously,but few report was about ESCC patients with neoadjuvant therapy.1 It is emergent to illustrate the comprehensive hallmarks of neoadjuvant therapy-resistance in ESCC at single cell level.

关键词： neoadjuvant squamous esophageal

light collection of the POLAR detector

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Science China(Physics,Mechanics & Astronomy) 2010年第S1期53卷 43-46页

作者： DONG YongWei,li yanguo,WU BoBing,ZHANG YongJie & ZHANG ShuangNan Key Laboratory of Particle Astrophysics,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing China

POLAR is a compact polarimeter dedicated to measuring the polarization of GRBs between 50-350 *** light collection of 200 mm×6 mm×6 mm plastic bars has been simulated and optimized in order to get uniform response to X-rays at different points along one single *** to the Monte Carlo results,the amplitude uniformity strongly depends on the level of polishing of the scintillator surface and the covering.A uniformity of 89% is achieved with a prototype constructed by a non position-sensitive PMT and an array of 4×4 bars.

关键词： plastic scintillator light collection gamma-ray burst polarimeter

SVOM gamma ray monitor

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Science China(Physics,Mechanics & Astronomy) 2010年第S1期53卷 40-42页

作者： DONG YongWei,WU BoBing,li yanguo,ZHANG YongJie & ZHANG ShuangNan Key Laboratory of Particle Astrophysics,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China Key Laboratory of Particle Astrophysics Institute of High Energy Physics Chinese Academy of Sciences Beijing China

The space-based multi-band astronomical Variable Object Monitor(SVOM) mission is dedicated to the detection,localization and broad-band study of gamma-ray bursts(GRBs) and other high-energy transient *** gamma ray monitor(GRM) onboard is designed to observe GRBs up to 5 *** this instrument,one of the key GRB parameters,Epeak,can be easily measured in the hard X-ray *** can achieve a detection rate of 100 GRBs per year which ensures the scientific output of SVOM.

关键词： gamma-ray burst Epeak triple phoswich

Fabrication of Onion-like Carbon Using Nanodiamond by Annealing at Lower Temperature and Vacuum

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Journal of Wuhan University of Technology(Materials Science) 2009年第6期24卷 935-939页

作者：邹芹 State Key Laboratory of Metastable Materials Science and Technology Yanshan University

Onion-like carbon （OLC） was fabricated by annealing nanodiamond at 1000 ℃ for 2 hours in low vacuum （1 Pa）. The OLC was characterized by X-ray diffraction （XRD）, high resolution transmission electron microscopy （HRTEM）, Fourier transform infrared spectroscopy （FTIR）, Raman spectroscopy and differential scanning calorimetry （DSC）. The experimental results show that the OLC exhibits similarity to the original nanodiamond particles in shape. The size of the OLC is found to be approximately 5 nm. The transformation mechanism of the OLC from nanodiamond was discussed also.

关键词： nanodiamond onion-like carbon annealing

The High Energy X-ray telescope (HE) onboard the Insight-HXMT astronomy satellite

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Science China(Physics,Mechanics & Astronomy) 2020年第4期63卷 20-34页

作者： CongZhan liu YiFei Zhang XuFang li XueFeng Lu Zhi Chang ZhengWei li AiMei Zhang YongJie Jin HuiMing Yu Zhao Zhang MinXue Fu YiBao Chen JianFeng Ji YuPeng Xu JingKang Deng RenCheng Shang GuoQing liu FangJun Lu ShuangNan Zhang YongWei Dong TiPei li Mei Wu yanguo li HuanYu Wang BoBing Wu YongJie Zhang Zhi Zhang Shaolin Xiong Yuan liu Shu Zhang HongWei liu YiJung Yang Fan Zhang Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Beijing 100049China

The Insight-Hard X-ray Modulation Telescope(Insight-HXMT) is a broadband X-ray and γ-ray(1-3000 ke V) astronomy satellite. One of its three main telescopes is the High Energy X-ray telescope(HE). The main detector plane of HE comprises 18 Na I(Tl)/Cs I(Na) phoswich detectors, where Na I(Tl) is used as the primary detector to measure ~ 20-250 ke V photons incident from the field of view(FOV) defined by collimators, and Cs I(Na) is used as the active shielding detector to Na I(Tl) by pulse shape discrimination. Additionally, Cs I(Na) is used as an omnidirectional γ-ray monitor. The HE collimators have a diverse FOV,i.e. 1.1°×5.7°(15 units), 5.7°×5.7°(2 units), and blocked(1 unit). Therefore, the combined FOV of HE is approximately5.7°×5.7°. Each HE detector has a diameter of 190 mm resulting in a total geometrical area of approximately 5100 cm2, and the energy resolution is ~15% at 60 ke V. For each recorded X-ray event by HE, the timing accuracy is less than 10 μs and the deadtime is less than 10 μs. HE is used for observing spectra and temporal variability of X-ray sources in the 20-250 ke V band either by pointing observations for known sources or scanning observations to unveil new sources. Additionally, HE is used for monitoring the γ-ray burst in 0.2-3 Me V band. This paper not only presents the design and performance of HE instruments but also reports results of the on-ground calibration experiments.

关键词： X-ray telescope HXMT calibration scintillation detector