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The direct and gut microbiota-mediated effects of dietary bile acids on the improvement of gut barriers in largemouth bass(Micropterus salmoides)

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Animal Nutrition 2023年第3期14卷 32-42页

作者： Rui Xia Qingshuang Zhang Dongmei Xia Qiang Hao Qianwen Ding Chao Ran yalin yang Aizhi Cao Zhen Zhang Zhigang Zhou China-Norway Joint Lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway-China Joint Lab on Fish Gut Microbiota Institute of BiologyNorwegian University of Science and TechnologyTrondheimNorway Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Shandong Longchang Animal Health Care Co. Ltd.Jinan 251100China Institute of Animal Husbandry and Veterinary Jiangxi Academy of Agricultural ScienceJiangxi 330000China

Fish gut barrier damage under intensive culture model is a significant concern for aquaculture *** study aimed to investigate the effects of bile acids(BAs)on gut barriers in Micropterus salmoides.A germ-free(GF)zebrafish model was employed to elucidate the effects of the direct stimulation of BAs and the indirect regulations mediated by the gut microbiota on gut barrier *** diets were formulated with BAs supplemented at 0,150,300 and 450 mg/kg,and these 4 diets were defined as control,BA150,BA300 and BA450,*** 5 weeks of feeding experiment,the survival rate of fish fed with BA300 diet was increased(P0.05).The results of the gut microbiota transferring experiment demonstrated an upregulation of gut barrier-related genes,including immunoglobulin Z/T(Ig Z/T),IL-6,IL-1βand IL-10,by the gut microbiota transferred from the BA300 group compared with the control(P<0.05).Feeding the BA300 diet directly to GF zebrafish resulted in enhanced expression of Ig M,Ig Z/T,lysozyme

关键词： Bile acids Gut barrier Gut microbiota Largemouth bass Germ-free zebrafish

Effects of dietary yeast nucleotides on growth, non-specific immunity intestine growth and intestinal microbiota of juvenile hybrid tilapia Oreochromis niloticus ♀ × Oreochromis aureus ♂

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Animal Nutrition 2015年第3期1卷 244-251页

作者： Li Xu Chao Ran Suxu He Jianli Zhang Jun Hu yalin yang Zhenyu Du Yanou yang Zhigang Zhou Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Feed Research Institute Chinese Academy of Agricultural Sciences Science of Animal and Technology College Anhui Agricultural University School of Life Sciences East China Normal University

This study investigated the effect of dietary supplementation of yeast nucleotides on the growth, nonspecific immunity, intestine growth and intestinal microbiota of juvenile hybrid tilapia. Tilapia(initial average weight of 8.02 g) was fed test diets supplemented with a yeast-originated nucleotide mixture(0,0.15, 0.30, 0.60, and 1.20 g/100 g diet) for 8 weeks. Fish fed the diet with 0.60% nucleotide had significantly higher weight gain than the control group(P < 0.05). Feed efficiency was improved in the fish fed 0.60 and 1.20% nucleotide compared with that in the control group. The optimal doses of nucleotides supplementation for growth and feed efficiency of fish were determined as 0.63 and 0.81%, *** growth was improved in the 0.30 and 0.60% groups, as indicated by significant increase in intestine length. The fish fed 0.60 and 1.20% nucleotide showed higher super oxide dismutase(SOD)activity and lower malondialdehyde(MDA) level in the liver than the control fish, indicating enhancement of the anti-oxidant status. Serum lysozyme activity was significantly increased in the 0.15 and 0.3%nucleotide supplementation groups, suggesting an enhancement effect on the non-specific immune response. Lastly, dietary nucleotides supplementation exerted moderate influence on the intestinal microbiota of hybrid tilapia. A reduction in the cumulative abundance of putative butyrate-producing species was observed in the intestinal microbiota of fish fed diets with 0.60% nucleotide compared with the control, implying an interaction between dietary nucleotides and butyrate production. Briefly, dietary supplementation with 0.60% nucleotide improve the growth performance, immune activity and intestine growth in tilapia.

关键词： Nucleotides Growth performance Hybrid tilapia Intestinal microbiota Denaturing gradient gel electrophoresis

Interactions between commensal bacteria and viral infection:insights for viral disease control in farmed animals

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Science China(Life Sciences) 2021年第9期64卷 1437-1448页

作者： Chao Ran Yu Li Xufa Ma Yadong Xie Mingxu Xie Yuting Zhang Wei Zhou yalin yang Zhen Zhang Li Zhou Kaijian Wei Zhigang Zhou Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Feed Research InstituteChinese Academy of Agricultural SciencesBeijing 100081China Sino-Norway Joint Lab on Fish Gut Microbiota Feed Research InstituteChinese Academy of Agricultural SciencesBeijing 100081China College of Fisheries Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyThe Innovation Academy of Seed DesignChinese Academy of SciencesWuhan 430072China

Viral diseases cause serious economic loss in farmed animals ***,the efficacy of remedies for viral infection in farmed animals is limited,and treatment strategies are generally lacking for aquatic *** of commensal microbiota and viral infection have been studied in recent years,demonstrating a third player in the interaction between hosts and ***,we discuss recent developments in the research of interactions between commensal bacteria and viral infection,including both promotion and inhibition effect of commensal bacteria on viral pathogenesis,as well as the impact of viral infection on commensal *** antiviral effect of commensal bacteria is mostly achieved through priming or regulation of the host immune responses,involving differential microbial components and host signaling pathways,and gives rise to various antiviral ***,we summarize studies related to the interaction between commensal bacteria and viral infection in farmed animals,including pigs,chickens,fish and invertebrate *** studies in this area will deepen our understanding of antiviral immunity of farmed animals in the context of commensal microbiota,and promote the development of novel strategies for treatment of viral diseases in farmed animals.

关键词： virus intestinal microbiota commensal bacteria farmed animals probiotics

The Fish Microbiota:Research Progress and Potential Applications

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Engineering 2023年第10期29卷 137-146页

作者： Yinyin Luan Ming Li Wei Zhou Yuanyuan Yao yalin yang Zhen Zhang Einar Ringø Rolf Erik Olsen Jihong Liu Clarke Shouqi Xie Kangsen Mai Chao Ran Zhigang Zhou China–Norway Joint Lab on Fish Gastrointestinal Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway–China Joint Lab on Fish Gastrointestinal Microbiota Institute of BiologyNorwegian University of Science and TechnologyTrondheim 7491Norway NIBIO Norwegian Institute of Bioeconomy ResearchÅs 1431Norway State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Key Laboratory of Mariculture(Ministry of Education) Ocean University of ChinaQingdao 266003China

The gut microbiota plays an important role in host health and *** understanding of the fish microbiota lags far behind our knowledge of that of humans and other ***,research has highlighted the importance of the microbiota in the health,performance,and various physiological functions of *** microbiota has been studied in various fish species,including model animals,economic fish,and wild fish *** composition of the fish microbiota depends on host selection,diet,and environmental *** intestinal microbiota affects the nutritional metabolism,immunity,and disease resistance of the fish host,while the host regulates the intestinal microbiota in a reciprocal way through both immune and non-immune *** and novel gnotobiotic fish models have been developed,which are important for the mechanistic study of host–microbiota interactions in *** this review,we discuss recent progress in fish microbiota *** describe various aspects of this research,including both studies on fish microbiota variations and fundamental research extending our knowledge of host–microbiota interaction in *** on how fish microbiota research may benefit fish health and industrial sustainability are also discussed.

关键词： Gut microbiota Fish Host-microbiota interaction Aquaculture

Research advances in the structure,function,and regulation of the gill barrier in

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Water Biology and Security 2023年第2期2卷 13-22页

作者： Xingyu Chen Shubin Liu Qianwen Ding Tsegay Teame yalin yang Chao Ran Zhen Zhang Zhigang Zhou Sino-Norway Joint Lab on Fish Gut Microbiota Feed Research InstituteChinese Academy of Agricultural SciencesBeijing100081China Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Feed Research InstituteChinese Academy of Agricultural SciencesBeijing100081China

The gill is an important organ in fish that is involved in various physiological functions,including respiration,filter-feeding,ammonia nitrogen excretion,and *** physiological functions of gills are closely related to their structure,external feeding conditions,and environmental ***,we first briefly summarize the basic structure and functions of the gills of teleosts,then explain the structure and function of the gill barrier(as a microbial barrier,a chemical barrier,a physical barrier,and an immune barrier)in ***,we introduce the regulation of fish gill barrier health by ubiquitous feeding factors and environmental factors,and provide an outlook on the prospects of gill barrier *** found that improving health through modulating feed factors is more convenient than improving environmental *** review increases our understanding of how nutrient composition and level in fish diet influences gill health and overall growth performance of fish.

关键词： Fish gill Gill barrier Feed factor Environmental factor Gill microbiota

Effects of solid-state fermentation product of yeast supplementation on liver and intestinal health,and resistance of common carp(Cyprinus carpio)against spring viraemia carp virus

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Animal Nutrition 2024年第3期18卷 408-418页

作者： Mengxin Wang Dongmei Xia Lijuan Yu Qiang Hao Mingxu Xie Qingshuang Zhang Yajie Zhao Delong Meng yalin yang Chao Ran Tsegay Teame Zhen Zhang Zhigang Zhou China-Norway Joint Lab on Fish Gut Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Hubei Key Laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic UniversityWuhan 430000China Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Tigray Agricultural Research Institute Mekelle Agricultural Research CenterMekelleTigray 251Ethiopia

This study aimed to investigate the effects of solid-state fermentation products of yeast(SFPY)on liver and intestinal health and disease resistance of common carp(Cyprinus carpio).A total of 200 common carp with an initial average weight of 2.55±0.004 g were divided into 5 groups(4 replications per group and 10 fish per replication),and were fed with one of five diets,including a control diet and 4 diets supplemented with 2‰(Y2),3‰(Y3),4‰(Y4),or 5‰(Y5)SFPY,respectively,for 8 *** indicated that,the addition of SFPY to the diet of common carp did not affect the growth performance or survival rate of fish(P=0.253).Interestingly,with the addition of SFPY,the triacylglycerol(TAG)content of the liver presented a linear decreasing tendency(P=0.004),with significantly decreased in Y4 and Y5 groups(P=0.035)compared with *** lipopolysaccharide(LPS)content and diamine oxidase(DAO)activity presented a negative linear relationship with the addition of SFPY(P=0.015,P=0.030),while serum lipopolysaccharide binding protein(LBP)content first decreased and then increased(P<0.001).The total antioxidant capacity(T-AOC)in the intestine of fish increased continuously with increasing SFPY supplementation(P=0.026),reaching the highest level in Y5 *** villus height in all experimental groups were significantly higher than that in the control group(P<0.001).Furthermore,compared to the control,adding 3‰SFPY to the control diet of common carp significantly increased the relative abundance of Fusobacteria(P=0.018)and decreased that of Proteobacteria(P=0.039)at phylum level,and increased the relative abundance of Cetobacterium(P=0.018)and decreased that of Shewanella(P=0.013)at genus *** with the control,the relative mRNA expression level of spring viraemia of carp virus N protein(SVCV-n)in the kidney was lower than that of the control group without significance and bottomed out in Y4 group(P=0.138).In conclusion,dietary SFPY enhanced the SVCV resistance capa

关键词： Yeast Solid-state fermentation product Probiotic Immunity Gut microbiota Common carp

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略论新时期小城镇建设的战略问题

理论前沿 2007年第12期 29-30页

作者：杨雅琳武汉大学政治与公共管理学院

发展小城镇要适应全面建设小康社会和社会主义新农村建设的要求,以科学发展观为统领,贯彻构建社会主义和谐社会的理念,站在对历史负责的高度,依靠科学的城乡规划来加强对小城镇建设的调控,从战略上把握新时期小城镇建设的路径,从而系统... 详细信息

发展小城镇要适应全面建设小康社会和社会主义新农村建设的要求,以科学发展观为统领,贯彻构建社会主义和谐社会的理念,站在对历史负责的高度,依靠科学的城乡规划来加强对小城镇建设的调控,从战略上把握新时期小城镇建设的路径,从而系统有序地推进小城镇持续发展。

关键词：新农村小城镇战略问题

Gene cloning,expression and characterization of an exo-chitinase with high β-glucanase activity from Aeromonas veronii B565

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Gene cloning,expression and characterization of an exo-chiti...

Cloning and expression of a novel chitinase gene from Citrobacter sp. P isolated from the intestine content of large yellow croakers and characterization of its protein product, an exo-chitinase with broad pH stability

中国生物工程学会第六次全国会员代表大会暨第九届学术年会

作者：霍凤敏冉超 yalin yang Jun Hu Zhigang Zhou 首都医科大学附属北京胸科医院中国农业科学院饲料研究所 Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Feed Research InstituteChinese Academy of Agricultural Sciences

Chitinases(EC 3.***.1.14) are the primary enzymes that catalyze the hydrolysis of recalcitrant chitin into valuable soluble *** have a very broad range of applications in ***,the use of chitinase in aquafeed has rarely been *** veronii strain B565 is a gram-negative bacterium isolated from aquaculture pond sediment in China,and was found to have the ability to produce chitinase to control fungal or Myxozoa-related *** B565 encodes five chitinases,namely Chi92(AEB48885.1),chitinase A(AEB48887.1),ChiB565(AEB48892.1),and two other chitinases(AEB48889.1,AEB50059.1).In this study,the chitinase gene chi92 was cloned from *** strain B565 and expressed in Pichia pastoris *** recombinant chitinase(Chi92) was purified and the biochemical properties were ***92 was supplemented in diets containing *** powder and fed to zebrafish for 14 *** comparing with the control group,effect of Chi92 supplementation on growth,feed utilization,microvilli morphology,and disease resistance was *** complete gene sequence encoded a polypeptide with 864 amino *** sequence alignment indicated that Chi92 contained the consensus substrate binding sequence SXGG(Ser270-Val271-Gly272-Gly273) and catalytic sequence DXXDXDXE(Asp307-Gly308-Val309-Asp310-Ile311-Asp312-Trp313-Glu314) of family 18 ***92 was successfully expressed in *** with high chitinase activity(69.4 U/mL).Chi92 exhibited optimal activity at pH 6.0 and 40 °***92 was resistant to protease trypsin,or pepsin and sensitive to chymotrypsin or tilapia intestinal *** Chi92 activity was increased by Mn2+(5 mmol/L) and mercaptoethanol(1 mmol/L),and strongly inhibited by Cr,Cu,and Fe(1 mmol/L or 5 mmol/L),and EDTA(5 mmol/L).Thin-layer chromatography and electrospray ionization–coupled mass spectrometry revealed that N-diacetylglucosamine was the dominant product of Chi92 when colloidal chitin was

关键词： Chitinase Chi92 Aeromonas veronii B565 Zebrafish

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Cloning and expression of a novel chitinase gene from Citrob...