Expression of Aspergillus niger 9891 Endoinulinase in Pichia pastoris

作     者：王建华 TENG Da YAO Yi Yang Yalin Zhang Fan 

作者机构：Laboratory of Gene Engineering Feed Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Department of Pharmaceutical Science University of Toronto Toronto Ontario M5S 2S2 CANADA 

出 版 物：《High Technology Letters》 (高技术通讯（英文版）)

年 卷 期：2004年第10卷第4期

页      面：52-56页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：国家高技术研究发展计划(863计划) 国家自然科学基金 

主　　题：曲霉菌 菊粉酶 催化作用 水解反应 果糖 Aspergillus niger 9891 

摘      要：An endoinulinase gene from Aspergillus niger 9891 (CGMCC0991) has been expressed in Pichia pastoris GS115 using pPIC9 vector. The recombinant endoinulinase was highly expressed and the optimization of the expression in a 7 liter of fermentor has been investigated. In fermented broth, the concentration of protein secreted is 2.15 mg/ml. The activity of endoinulinase is 1501 U/ml with sucrose as substrate and 291 U/ml with inulin as substrate, 105 and 273 times higher than that from the original strain respectively.