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Taurine attenuates activation of hepatic stellate cells by inhibiting autophagy and inducing ferroptosis

World Journal of Gastroenterology 2024年第15期30卷 2143-2154页

作者： Sen Li qian-jun Ren Can-Hao Xie Yang Cui Li-Tao Xu yi-Dan Wang Su Li Xing-qiu Liang Bin Wen Ming-Kun Liang Xiao-Fang Zhao Department of Basic Science Guangxi University of Chinese MedicineNanning 541100Guangxi Zhuang Autonomous RegionChina Department of Science and Technology Ruikang Hospital Affiliated to Guangxi University of Chinese MedicineNanning 541100Guangxi Zhuang Autonomous RegionChina Traditional Chinese Medicine Specialty Office Ruikang Hospital Affiliated to Guangxi University of Chinese MedicineNanning 541100Guangxi Zhuang Autonomous RegionChina

BACKGROUND Liver fibrosis is a compensatory response during the tissue repair process in chronic liver injury,and finally leads to liver cirrhosis or even hepatocellular *** pathogenesis of hepatic fibrosis is associated with the progressive accumulation of activated hepatic stellate cells(HSCs),which can transdiffer-entiate into myofibroblasts to produce an excess of the extracellular matrix(ECM).Myofibroblasts are the main source of the excessive ECM responsible for hepatic ***,activated hepatic stellate cells(aHSCs),the principal ECM producing cells in the injured liver,are a promising therapeutic target for the treatment of hepatic *** To explore the effect of taurine on aHSC proliferation and the mechanisms *** Human HSCs(LX-2)were randomly divided into five groups:Normal control group,platelet-derived growth factor-BB(PDGF-BB)(20 ng/mL)treated group,mmol/L,respectively)with PDGF-BB(20 ng/mL)treated *** Counting Kit-8 method was performed to evaluate the effect of taurine on the viability of ***-linked immunosorbent assay was used to estimate the effect of taurine on the levels of reactive oxygen species(ROS),malondialdehyde,glutathione,and iron *** electron microscopy was applied to observe the effect of taurine on the autophagosomes and ferroptosis features in *** real-time polymerase chain reaction and Western blot analysis were performed to detect the effect of taurine on the expression ofα-SMA,Collagen I,Fibronectin 1,LC3B,ATG5,Beclin 1,PTGS2,SLC7A11,and *** Taurine promoted the death of aHSCs and reduced the deposition of the *** with taurine could alleviate autophagy in HSCs to inhibit their activation,by decreasing autophagosome formation,downregulating LC3B and Beclin 1 protein expression,and upregulating p62 protein ***,treatment with taurine triggered ferroptosis and ferritinophagy to eliminate aHSCs characterized b

关键词： Hepatic stellate cells Autophagy Ferroptosis Molecular docking Taurine

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The Total Synthesis of (±)-Hinesol and (±)-Agarospirol

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Chinese Chemical Letters 1997年第11期8卷 957-960页

作者： Jin jun WANG Chang Jin yue jun qiu (Department Of Fine Chemistry, Jilin Chemical Engineering College, Jilin, 132002)cang yi qian(Department of Chemistry, Qing Hua University, Beijing, 100084) Department of Fine Chemistry Jilin Chemical Engineering College Jilin 132002 China Department of Chemistry Qing Hua University Beijing 100084 China

A mild base-catalyzed retro-benzilic acid rearrangement of a proto-[2+2]photocycloadduct, formed in highly steroselective photoaddition of methyl 2,4-dioxo-pentanoate to 1,5-dimethyl-6-methylenecyclohexene, afforded a spiro[4, 5] decenedione from which hinesol and agarospirol were synthesized by means of reductive elimination of α-diketone and C1-homologation.

关键词： The Total Synthesis of Hinesol and Agarospirol

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NνDEx-100 conceptual design report

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Nuclear Science and Techniques 2024年第1期35卷 18-33页

作者： Xi-Guang Cao Yan-Long Chang Kai Chen Emilio Ciuffoli Li-Min Duan Dong-Liang Fang Chao-Song Gao Surja K.Ghorui Peng-Chong Hu qiang Hu Si-Yuan Huang Ze-Yu Huang Lei Lang Yu-Lan Li Zhi-Jie Li Tian-Yu Liang jun Liu Chen-Gui Lu Fa-Tai Mai Yuan Mei Hao qiu Xiang-Ming Sun Xiao-Xing Tang Hu-Lin Wang qian-Ming Wang Le Xiao Mu-Yun Xiao Jian-Yu Xin Nu Xu Peng Yang yi-Chen Yang Zhen Yang Zong-Yang Yu Dong-Liang Zhang jun-Wei Zhang Cheng-Xin Zhao Dou Zhu NνDEx-100 collaboration Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China Lanzhou University Lanzhou 730000China Central China Normal University Wuhan 430079China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Lawrence Berkeley National Laboratory BerkeleyCA 94720USA North China University of Water Resources and Electric Power Zhengzhou 450045China 不详

Observing nuclear neutrinoless double beta (0vββ) decay would be a revolutionary result in particle *** such a decay would prove that the neutrinos are their own antiparticles,help to study the absolute mass of neutrinos,explore the origin of their mass,and may explain the matter-antimatter asymmetry in our universe by lepton number *** propose developing a time projection chamber (TPC) using high-pressure ^(82)SeF_(6) gas and Topmetal silicon sensors for readout in the China Jinping Underground Laboratory (CJPL) to search for neutrinoless double beta decay of82Se,called the NvDEx *** being located at CJPL with the world’s thickest rock shielding,NvDEx combines the advantages of the high Qββ(2.996 MeV) of82Se and the TPC’s ability to distinguish signal and background events using their different topological *** makes NvDEx unique,with great potential for low-background and high-sensitivity 0 vββ***-100,a NvDEx experiment phase with 100 kg of SeF_(6)gas,is being built,with plans to complete installation at CJPL by *** report introduces 0 vββ physics,the NvDEx concept and its advantages,and the schematic design of NvDEx-100,its subsystems,and background and sensitivity estimation.

关键词： Neutrinoless double beta decay Time projection chamber ^(82)SeF_(6) China Jinping Underground Laboratory

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藏药高原毛茛质量标准研究

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西藏科技 2024年第4期46卷 13-21页

作者：邱毅兰钧达娃卓玛李倩西藏自治区食品药品检验研究院国家药品监督管理局中药(藏药)质量控制重点实验室拉萨850000 四川省药品检验研究院成都611731

目的修订提高藏药高原毛茛药材的质量标准,为高原毛茛的质量控制提供科学可靠的评价依据。方法对15批不同来源的高原毛茛药材进行性状、显微和薄层鉴别、水分、灰分和酸不溶性灰分检查、浸出物和化学成分初步分析。其中本试验中以槲... 详细信息

目的修订提高藏药高原毛茛药材的质量标准,为高原毛茛的质量控制提供科学可靠的评价依据。方法对15批不同来源的高原毛茛药材进行性状、显微和薄层鉴别、水分、灰分和酸不溶性灰分检查、浸出物和化学成分初步分析。其中本试验中以槲皮素作为高原毛茛药材薄层鉴别的对照品。结果高原毛茛药材显微特征明显;检测15批高原毛茛药材,样品薄层色谱图与槲皮素对照品色谱图一致,显相同颜色的荧光斑点;高原毛茛药材水分、总灰分、酸不溶性灰分和醇溶性浸出物范围分别为8.18%~11.01%、5.6~7.8%、0.69~1.20%、35.02%~39.04%;开展了本品种化学成分的初步分析研究,15批次样品均检出与槲皮素对照品和山柰素对照品保留时间相同的峰。结论本研究首次全面考察高原毛茛的各项质控指标,能有效评价高原毛茛的质量,为高原毛茛的质量控制提供参考。

关键词：高原毛茛质量标准质控指标显微鉴别

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Au@Ag@Pt core–shell nanorods regulating Ag release behavior endow titanium antibacterial activity and biocompatibility

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Rare Metals 2022年第2期41卷 630-638页

作者： Jia-jun qiu Ting-Ting Yang yi-Fan Li Wen-Hao qian Xuan-Yong Liu School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of Sciences School of Materials Science and Engineering Jiamusi University Smart Materials and Surfaces Laboratory Faculty of Engineering and EnvironmentNorthumbria University Shanghai Xuhui District Dental Center

Although titanium and its alloys are extensively used in orthopedics and dentistry fields,implant failures still happen because of implant-associated ***,Au@Ag@Pt core–shell nanorods with noble metal combination were fabricated and assembled on medical titanium surface and the antibacterial activity and biocompatibility were *** results showed that antibacterial rates of Ti–Au@Ag@Pt against *** and *** were 89.7%and 92.7%,***,Ti–Au@Ag@Pt showed no obvious cell toxicity with MC3 T3-E1 cells grew well on the sample *** was discovered that the Pt shell layer on Ti–Au@Ag@Pt slowed down the Ag ion release rate which endowed medical titanium surface with both antibacterial activity and good biocompatibility.

关键词： Titanium Silver Nanorod Antibacterial Biocompatibility

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髓内棒固定对先天性胫骨假关节患儿骺板骨桥发生的影响

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中华小儿外科杂志 2024年第1期45卷 57-64页

作者：许思宇梅海波朱光辉刘昆谭谦严安肖汉仇君简书浪蔡毅湖南省儿童医院骨科长沙410007

目的研究先天性胫骨假关节(congenital pseudarthrosis of tibia,CPT)患儿通过髓内棒经骺板固定治疗后,胫骨远端骺板骨桥的发生情况和影响因素。方法回顾性分析2011年9月至2020年12月收治于湖南省儿童医院骨科的209例采用髓内棒固定治疗... 详细信息

目的研究先天性胫骨假关节(congenital pseudarthrosis of tibia,CPT)患儿通过髓内棒经骺板固定治疗后,胫骨远端骺板骨桥的发生情况和影响因素。方法回顾性分析2011年9月至2020年12月收治于湖南省儿童医院骨科的209例采用髓内棒固定治疗的CPT患儿临床资料,其中男133例,女76例;手术时年龄为(3.8±2.6)岁,范围在1.0~14.7岁。术后随访(4.7±2.2)年,定期复查X线片观察患儿是否发生骨桥,根据末次随访时是否发生骨桥将患儿分为骨桥组(13例)和无骨桥组(196例),比较两组患儿术前、术中及术后随访的观察指标,通过单因素分析和倾向性评分匹配探究骨桥发生的影响因素。结果患儿骨桥发生率为6.2%(13/209),骨桥发生中位时间为11.0(7.0,14.5)个月,范围在术后2.0~17.0个月。两组患儿髓内棒固定的次数比较,差异有统计学意义(P=0.014),即髓内棒固定累计次数≥3次时,CPT患儿术后骨桥发生的可能性较0.05),进一步行倾向性评分匹配后未发现其他影响骨桥发生的因素。结论CPT患儿髓内棒固定术后骨桥发生率低,髓内棒固定累计次数是术后骨桥发生的影响因素。

关键词：假关节先天性胫骨假关节骺板

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重组酶聚合酶扩增技术检测南美白对虾急性肝胰腺坏死病

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农业生物技术学报 2024年第4期32卷 961-973页

作者：金东升漏懿荣王瑶华段丽君李其昂陈琛闫茂仓陈炯周前进宁波大学农产品质量安全危害因子与风险防控国家重点实验室宁波大学海洋学院浙江省海洋水产养殖研究所浙江省近岸水域生物资源开发与保护重点实验室

急性肝胰腺坏死病(acute hepatopancreatic necrosis disease, AHPND)是一种新近出现的对虾(Penaeidae)细菌性疾病，给世界范围内的对虾养殖业造成了巨大的经济损失。AHPND主要由携带毒力基因pirA和pirB的弧菌(Vibrio spp.)引起。本研究... 详细信息

急性肝胰腺坏死病(acute hepatopancreatic necrosis disease, AHPND)是一种新近出现的对虾(Penaeidae)细菌性疾病，给世界范围内的对虾养殖业造成了巨大的经济损失。AHPND主要由携带毒力基因pirA和pirB的弧菌(Vibrio spp.)引起。本研究以pirA和pirB为检测靶标，分别设计和筛选特异性引物和探针，建立重组酶聚合酶扩增(recombinase polymerase amplification, RPA)方法。RPA反应在39℃下进行，反应时间设定为20 min。结果显示，针对pirA和pirB基因分别筛选到1套引物/探针组合。应用该引物/探针组合对RPA方法进行系列测试，发现引物/探针组合均能够特异性检出目标毒力基因，对溶藻弧菌(Vibrio alginolyticus)、副溶血弧菌(V. parahaemolyticus)，以及虾肝肠孢虫(Enterocytozoon hepatopenaei)等13种微生物的基因组DNA未出现扩增。灵敏度实验结果发现，使用pirA的引物/探针组合最低能够稳定检测出0.01 fg/μL的重组质粒pirAB-pMD19，而使用pirB的引物/探针组合最低能够稳定检测出0.1 fg/μL的重组质粒pirAB-pMD19。经优化，检测pirA和pirB的RPA反应的最优引物浓度为0.4μmol/L，探针浓度为0.16μmol/L。使用优化的引物/探针组合对可疑南美白对虾(Penaeus vannamei)组织样品利用RPA方法进行检测，结果显示，利用建立的RPA方法能够准确地从对虾组织样品中检测到pirA和pirB，综合检测结果与世界动物卫生组织(World Organisation for Animal Health, WOAH)推荐的PCR方法结果一致。综上，本研究分别针对pirA和pirB建立的PRA方法能够快速、准确地检出ANPND病原，而且操作简便、耗时短，有望成为AHPND病害日常监测和快速检测的重要补充。

关键词：重组酶聚合酶技术(RPA) 急性肝胰腺坏死病(AHPND) pirA pirB 检测

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MANAGEMENT OF CEREBROSPINAL FLUID LEAKAGE FOLLOWING CERVICAL SPINE SURGERY

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Chinese Medical Sciences Journal 2008年第2期23卷 121-125页

作者： Ye Tian Ke-yi Yu yi-peng Wang jun qian Gui-xing qiu Department of Orthopaedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆Peking Union Medical College Beijing 100730

Objective To investigate the management and outcome of cerebrospinal fluid leakage(CSFL)after cervical *** Medical records of 642 patients who underwent cervical surgery between December 1999 and December 2005 at our hospital were retrospectively *** patients complicated by CSFL after surgery were enrolled,of which 4 cases were complicated after ossified posterior longitudinal ligament or posterior vertebral osteophyte resection directly injuring the dura,and 1 case after posterior cervical double-door laminoplasty without observed dural injury during *** the 5 CSFL cases,4 cases occurred at 1-3 days after operation and 1 case at 9 days after *** 5 postoperative CSFL cases were treated through wound drainage removal,wound sutures,prophylactic antibiotics,and continuous subarachnoid drainage in the elevated head *** All 5 CSFL cases experienced leakage cessation within 1-3 days and wound healing within 4-8 days,and subarachnoid drainage lasted 11-16 days with an average volume of 320 mL(range,150-410 mL).Four cases experienced headache,nausea and vomiting,1 case suffered from somnolence and hyponatremia,and symptoms subsided after symptomatic treatment and intravenous fluid *** patients were followed up for an average of 32 months(range,22-50 months).No occurrence of cerebrospinal fluid cyst or wound infection was *** produced no significant negative effects upon neuromuscular function *** Continuous subarachnoid cavity drainage in combination with elevated head position is a simple and safe non-surgical method in treatment of CSFL following cervical surgery.

关键词： cervical vertebra cerebrospinal fluid leakage subarachnoid drainage

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A new cell death program regulated by toll-like receptor 9 through p38 mitogen-activated protein kinase signaling pathway in a neonatal rat model with sepsis associated encephalopathy

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Chinese Medical Journal 2022年第12期135卷 1474-1485页

作者： Ruixi Zhou junjie ying Xia qiu Luting Yu Yan yue qian Liu Jing Shi Xihong Li yi Qu Dezhi Mu Department of Pediatrics West China Second University HospitalSichuan UniversityChengduSichuan 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children Sichuan UniversityMinistry of EducationChengduSichuan 610041China

Background:Sepsis,a serious condition with high mortality,usually causes sepsis associated encephalopathy(SAE)that involves neuronal cell ***,the cell death programs involved and their underlying mechanisms are not *** study aimed to explore the regulatory mechanisms of different cell death programs in ***:A neonatal rat model of SAE was established by cecal ligation and *** rate and vital signs(mean arterial pressure and heart rate)were monitored,nerve reflexes were evaluated,and cortical pathological changes were observed by hematoxylin and eosin *** expression of pyroptosis,apoptosis,and necroptosis(PANoptosis)-related proteins,mitogen-activated protein kinase(MAPK),and its upstream regulator toll-like receptor 9(TLR9)were *** expression of TLR9 in neurons was observed by immunofluorescence *** ultrastructure of neurons was observed by transmission electron ***:First,PANoptosis was found in cortical nerve cells of the SAE ***,the subunits of MAPKs,p38 MAPK,jun N-terminal kinase,and extracellular signal-regulated kinase(ERK)were *** pharmacologically inhibiting each of the subunits,only p38 MAPK was found to be associated with ***,blocking the p38 MAPK signaling pathway activated necroptosis but inhibited apoptosis and *** necroptosis was pharmacologically inhibited,apoptosis and pyroptosis were ***,we found that the expression of TLR9,a regulator of MAPKs,was significantly increased in this *** down-regulation of TLR9,p38 MAPK,and ERK signaling pathways were inhibited,which led to the inhibition of *** analysis found that down-regulation of TLR9 improved the survival rate and reduced the pathological changes in SAE ***:Our study showed that the programs comprising PANoptosis are activated simultaneously in SAE ***9 activated PANoptosis through the p38 MAPK signaling pathw

关键词： Sepsis associated encephalopathy TLR9 Apoptosis Pyroptosis Necroptosis p38 mitogen-activated protein kinase

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甘薯和芋头在闽东山羊瘤胃中的降解特性及表面附着微生物群落变化

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草业学报 2024年第9期33卷 169-184页

作者：顾明明姜幸慧马志毅邱水玲刘浩宇张洺瑞卢佳宁丘宇俊王本治甘乾福福建农林大学动物科学学院(蜂学学院) 福建福州350002

本试验旨在研究甘薯和芋头在闽东山羊瘤胃中的降解特性及表面附着微生物群落动态变化。选用4头14月龄健康装有永久瘤胃瘘管的闽东公山羊[平均体重(26.60±2.35)kg],采用尼龙袋法分别在2、4、8、16、24、36和48 h测定甘薯和芋头的营养成... 详细信息

本试验旨在研究甘薯和芋头在闽东山羊瘤胃中的降解特性及表面附着微生物群落动态变化。选用4头14月龄健康装有永久瘤胃瘘管的闽东公山羊[平均体重(26.60±2.35)kg],采用尼龙袋法分别在2、4、8、16、24、36和48 h测定甘薯和芋头的营养成分的动态降解率及降解过程中表面附着微生物变化。结果表明:1)甘薯和芋头的瘤胃降解率随滞留时间的延长均逐渐升高。干物质(DM)有效降解率分别为66.48%和62.88%,粗蛋白(CP)有效降解率分别为34.28%和32.62%,淀粉有效降解率分别为53.44%和43.60%,其中甘薯淀粉有效降解率显著高于芋头(P0.05)。门水平上,两组间优势菌均为拟杆菌门和厚壁菌门。属水平上,两组间优势菌属均为普雷沃氏菌属。Mantel检验进一步表明,假丁酸弧菌属与甘薯和芋头瘤胃降解率均呈显著正相关(r>0,P<0.05)。3)功能预测分析表明,甘薯和芋头通过磷酸戊糖途径、乙醛酸和二羧酸代谢以及丙酸代谢调控瘤胃微生物相互作用利用淀粉的功能。综上所述,甘薯和芋头具有较好的瘤胃降解性能,其中甘薯DM和淀粉降解率更佳。在降解过程中,两种饲料均不会破坏瘤胃微生物的动态平衡,因此甘薯和芋头是具有潜力的精饲料资源。

关键词：甘薯芋头闽东山羊瘤胃降解率瘤胃微生物

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