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Aurora B kinase activation requires survivin priming phosphorylation by PLK1

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Journal of Molecular Cell Biology 2011年第4期3卷 260-267页

作者： youjun Chu Phil y.yao Wenwen Wang Dongmei Wang Zhikai Wang Liangyu Zhang yuejia Huang yuwen Ke Xia Ding Xuebiao yao Anhui Key Laboratory of Cellular Dynamics and Chemical Biology University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA Department of Medicine Beijing University of Chinese MedicineBeijing 100086China

During cell division,chromosome segregation is orchestrated by the interaction of spindle microtubules with the *** attachment of spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin,INCENP and survivin(SUR).The current working model argues that SUR is responsible for docking Aurora B to the centromere whereas its precise role in Aurora B activation has been ***,we show that Aurora B kinase activation requires SUR priming phosphorylation at Ser20 which is catalyzed by polo-like kinase 1(PLK1).Inhibition of PLK1 kinase activity or expression of non-phosphorylatable SUR mutant prevents Aurora B activation and correct spindle microtubule *** PLK1-mediated regulation of Aurora B kinase activity was examined in real-time mitosis using fluorescence resonance energy transfer-based reporter and quantitative analysis of native Aurora B substrate *** reason that the PLK1-mediated priming phosphorylation is critical for orchestrating Aurora B activity in centromere which is essential for accurate chromosome segregation and faithful completion of cytokinesis.

关键词： Aurora B kinase sensor phospho-CENP-A PLK1 survivin

Mechanical Relaxation of a Ti36.2Zr30.3Cu8.3Fe4Be21.2 Bulk Metallic Glass: Experiments and Theoretical Analysis

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Acta Metallurgica Sinica(English Letters) 2019年第6期32卷 726-732页

作者： J.C.Qiao y.H.Chen G.J.Lyu K.K.Song J.M.Pelletier y.yao School of Mechanics Civil Engineering and ArchitectureNorthwestern Polytechnical UniversityXi'an 710072China School of Mechanical Electrical and Information EngineeringShandong University(Weihai)Weihai 264209China MATEIS UMR CNRS5510Bat.B.PascalINSA-LyonUniversite de Lyon69621 Villeurbanne CedexFrance

The dynamic mechanical relaxation behavior of Ti36.2Zr30.3Cu8.3Fe4Be21.2 bulk metallic glass with good glass-forming ability was investigated by mechanical *** mechanical relaxation behavior was analyzed in the framework of quasi-point defects *** experimental results demonstrate that the atomic mobility of the metallic glass is closely associated with the correlation factorχ.The physical aging below the glass transition temperature T g shows a non-Debye relaxation behavior,which could be well described by stretched Kohlrausch exponential *** Kohlrausch exponentβaging reflects the dynamic heterogeneities of the metallic *** concentration of"defects"and atomic mobility decrease caused by the in situ successive heating during the mechanical spectroscopy experiments.

关键词： Metallic glasses Mechanical relaxation Physical aging Quasi-point defects

Electronic structure of GaAs/A1GaAs quantum double rings in lateral electric field

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Chinese Optics Letters 2009年第10期7卷 882-885页

作者： y.yao T.Ochiai T.Mano T.Kuroda T.Noda N.Koguchi K.Sakoda National Institute for Materials Science University of Tsukuba L-NESS Universit di Milano Bicocca

A three-dimensional model of GaAs/A1GaAs quantum double rings in the lateral static electric field is investigated theoretically. The eigenvalue problem with the effective-mass approximation is solved by means of the finite-element method. The energy levels and wave functions of quantum-confined electrons and heavy holes are obtained and show an agreement with our previous theoretical and experimental studies. It is shown in the approximation of neglecting the Coulomb attraction between the electron and heavy hole that a relatively large Stark shift of exciton emission of 4 meV is attainable with an applied electric field of 0.7 kV/cm.

关键词： GaAs Electronic structure of GaAs/A1GaAs quantum double rings in lateral electric field

3D-Stacked Multistage Inertial Microfluidic Chip for High-Throughput Enrichment of Circulating Tumor Cells

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Cyborg and Bionic Systems 2022年第1期2022卷 200-209页

作者： X.Xu X.Huang J.Sun J.Chen G.Wu y.yao N.Zhou S.Wang L.Sun Ministry of Education Key Laboratory of RF Circuits and Sytems Hangzhou Dianzi UniversityHangzhou310018 ZhejiangChina Institute for Translational Medicine Zhejang UniversityHangzhou310029 ZhejiangChina Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province School of EngineeringWestlake UniversityHangzhou310024 Zhejiang China National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610065China Clinical Research Center for Respiratory Disease West Chima HospitalSichuan UniversityChengdu 610065China Institute for Advanced Study Chengdu UniversityChengdu 610106China

Whether for cancer diagnosis or single-cell analysis,it remains a major dhallenge to isolate the target sample cells from a large background cell for high-efficiency downstream detection and analysis in an integrated ***,in this paper,we propose a 3D stacked multistage inertial microfluidic sorting chip for high-throughput enrichment of circulating tumor cells(CTCs)and convenient downstream *** this chip,the first stage is a spiral channel with a trapezoidal cross-section,which has better separation performance than a spiral channel with a rectangular *** second and third stages adopt symmetrical square serpentine channels with different rectangular cross-section widths for further separation and enrichment of sample cells reducing the outlet flow rate for easier downstream detection and *** multistage channel can separate 5 pum and 15 um particles with a separation efficiency of 92.37%and purity of 98.10%at a high inlet flow rate of 1.3 mL/***,it can separate tumor cells(SW480,A549,and Caki-1)from massive red blood cells(RBCs)with *** efficiency of>80%,separation purity of>90%,and a concentration fold of~*** proposed work is aimed at providing a high-throughput sample processing system that can be easily integrated with flowing sample detection methods for rapid CTC analysis.

关键词： fluid diagnosis separation

Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

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Journal of Molecular Cell Biology 2020年第8期12卷 654-665页

作者： Xu Liu Leilei Xu Junying Li Phil y.yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng yan Wenwen Wang Xing Liu Xuebiao yao MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical Biology CAS Center for Excellence in Molecular Cell ScienceHefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of ChinaHefei 230027China Department of Physiology Morehouse School of MedicineAtlantaGA 30310USA School of Basic Medical Sciences Beijing University of Chinese MedicineBeijing 100029China Department of Biology Duke University DurhamNC 27708USA Institute of ProteoGenomics Beijing 100029China Harvard Medical School BostonMA 02115USA

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase ***,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains *** the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric *** sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during *** real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability *** biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic *** inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the ***,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone *** findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle *** CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

关键词： organoids.cell division central spindle CENP-E syntelin PRC1

Isopiestic measurement and solubility prediction of the ternary system MgCl2-NaCl-H2O

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Isopiestic measurement and solubility prediction of the tern...

Research on rat models of hypobaric hypoxia-induced pulmonary hypertension

第十七届全国化学热力学和热分析学术会议

作者： H.Han D.Li O.Dong L.Guo y.yao D.Zeng Qinghai Institute of Salt Lakes Chinese Academy of Sciences College of Chemistry and Chemical Engineering Central South University

The thermodynamic properties of the ternary system Mg Cl2-Na Cl-H2 O are instructive for magnesium chloride purification from crude bischofite in salt lakes. According to our experience, the sodium content in bischofite cannot be decreased by recrystallization method. In order to explain this abnormal phenomenon, we need profound understanding the multi-temperature phase diagram of the ternary system Mg Cl2-Na Cl-H2 O. Since the crystallization field of Mg Cl2·6H2O is very narrow, the determination of the eutectic line between the Mg Cl2·6H2O and Na Cl phases is a challenge. Although a lot of solubility experiments have been reported by many authors, a great discrepancy exists among them. So we determined the water activities of the system Mg Cl2-Na Cl-H2 O at 323.15 and 348.15 K, and constructed the phase diagrams and the eutectic line between the Mg Cl2·6H2O and Na Cl phases of the system Mg Cl2-Na Cl-H2 O with the Pitzer’s ion interaction model from 298.15 K to 373.15 K, as shown in Figure 1. We carried out purification test using a new fractional crystallization method according to the simulated results, the sodium content in bischofite decreased from 1.21 % to 0.17 %.

关键词： Solubility Water activity Osmotic Thermodynamic model

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Research on rat models of hypobaric hypoxia-induced pulmonar...

Ultrafast Martensitic Phase Transition Driven by Intense Terahertz Pulses

青藏铁路运营十周年学术研讨会

作者： T.-T.MA y WANG X.-L.ZHOU H.JIANG R.GUO L.-N.JIA H.CHANG y GAO X.-y.yao Z.-M.GAO L.PAN Department of Geriatric Medicine Beijing Shijitan HospitalCapital Medical University Department of Hypoxia Laboratory Beijing Shijitan HospitalCapital Medical University Department of Pathology Beijing Shijitan HospitalCapital Medical University Health Science Center of Peking University

OBJECTIVE:Rat models of hypobaric hypoxia-induced pulmonary hypertension are commonly used in studies of chronic mountain sickness,while there are few researches specially focusing on these rats *** study aims to exploring possible pathogenesis of hypobaric hypoxia-induced pulmonary hypertension by experimenting on hypobaric hypoxiainduced PH rat models at different *** AND METHODS:32 healthy male SD rats were randomly divided into six groups of different degree and time period of hypobaric *** mean pulmonary arterial pressure（m PAP）,right ventricular pressure（RVSP）,the right ventricle（RV）,left ventricular（LV）,ventricular septal（S）,the right ventricular hypertrophy index（RVHI）[calculated under the formula of RV/（LV + S）],hematoxylin-eosin staining,elastic fibers staining,the ratio of the thickness of vascular wall to its outer diameter（MT%）,the ratio of the cross-sectional area of the middle vascular wall to the total vascular cross-sectional area（MA%）;the a-SMA,and the Ki6 expressions were detected to evaluated the pulmonary ***:There were significant differences of the mPAP,RVSP and RVHI value between the hypobaric hypoxia groups and the control group（p ＜ 0.05）.The mPAP,RVSP,RVHI,MT%,MA%,α-SMA,and Ki6 of rats in model groups at an altitude of 3KM were higher than those of the control group,which raised gradually with the number of weeks *** mPAP,RVSP,RV/（LV + S） value,MT%,MA%,a-SMA,and Ki67 of the 5KM-4W group were significantly higher than those of the control group（p ＜ 0.05）.CONCLUSIONS:Rat models with pulmonary hypertension at different altitudes have been successfully established by automatic adjusting hypobaric hypoxia *** to a low oxygen environment at a simulate-altitude of 3km for 8 weeks have caused the pathological remodeling of pulmonary vascular walls and pulmonary hypertension,and further led to a series of pathological changes,including right ventricular ***

关键词： Hypobaric hypoxia Pulmonary hypertension Animal models

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Ultrafast Science 2023年第1期3卷 27-32页

作者： B.Q.Song X.yang C.Sundahi J-H.Kang M.Mootz y.yao I.E.Perakis L.Luo C.B.Eom J.Wang Department of Physics and Astronomy lowa State UniversityAmesIA 50011USA Ames National Laboratory of U.S.DOE AmesIA 50011USA Department of Materials Science and Engineering.University of Wisconsin-Madison MadisonWI 53706USA Department of Physics University of Alabama at BirminghamBirminghamAL35294-1170USA

We report on an ultrafast nonequilibrium phase transition with a strikingly long-lived martensitic anomaly driven by above-threshold single-cycle terahertz pulses with a peak field of more than 1 MV/cm.A nonthermal,terahertz-induced depletion of low-frequency conductivity in Nb_(3)Sn indicates increased gap splitting of high-energy Г_(12) bands by removal of their degeneracies,which induces the martensitic phase above their equilibrium transition *** contrast,optical pumping leads to a Г_(12) gap thermal *** light-induced nonequilibrium martensitic phase exhibits a substantially enhanced critical temperature up to~100 K,i.e.,more than twice the equilibrium temperature,and can be stabilized beyond technologically relevant,nanosecond time *** with first-principle simulations,we identify a compelling terahertz tuning mechanism of structural order via Г_(12) phonons to achieve the ultrafast phase transition to a metastable electronic state out of equilibrium at high temperatures far exceeding those for equilibrium states.

关键词： martensitic equilibrium nonequilibrium

Striatopallidal dysfunction underlies repetitive behavior in Shank3 model of autism

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Striatopallidal dysfunction underlies repetitive behavior in...