Research on rat models of hypobaric hypoxia-induced pulmonary hypertension
作者单位：Department of Geriatric MedicineBeijing Shijitan HospitalCapital Medical University Department of Hypoxia LaboratoryBeijing Shijitan HospitalCapital Medical University Department of PathologyBeijing Shijitan HospitalCapital Medical University Health Science Center of Peking University
会议名称：《青藏铁路运营十周年学术研讨会》
会议日期：2016年
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
关 键 词：Hypobaric hypoxia Pulmonary hypertension Animal models
摘 要：OBJECTIVE:Rat models of hypobaric hypoxia-induced pulmonary hypertension are commonly used in studies of chronic mountain sickness,while there are few researches specially focusing on these rats *** study aims to exploring possible pathogenesis of hypobaric hypoxia-induced pulmonary hypertension by experimenting on hypobaric hypoxiainduced PH rat models at different *** AND METHODS:32 healthy male SD rats were randomly divided into six groups of different degree and time period of hypobaric *** mean pulmonary arterial pressure（m PAP）,right ventricular pressure（RVSP）,the right ventricle（RV）,left ventricular（LV）,ventricular septal（S）,the right ventricular hypertrophy index（RVHI）[calculated under the formula of RV/（LV + S）],hematoxylin-eosin staining,elastic fibers staining,the ratio of the thickness of vascular wall to its outer diameter（MT%）,the ratio of the cross-sectional area of the middle vascular wall to the total vascular cross-sectional area（MA%）;the a-SMA,and the Ki6 expressions were detected to evaluated the pulmonary ***:There were significant differences of the mPAP,RVSP and RVHI value between the hypobaric hypoxia groups and the control group（p 0.05）.The mPAP,RVSP,RVHI,MT%,MA%,α-SMA,and Ki6 of rats in model groups at an altitude of 3KM were higher than those of the control group,which raised gradually with the number of weeks *** mPAP,RVSP,RV/（LV + S） value,MT%,MA%,a-SMA,and Ki67 of the 5KM-4W group were significantly higher than those of the control group（p 0.05）.CONCLUSIONS:Rat models with pulmonary hypertension at different altitudes have been successfully established by automatic adjusting hypobaric hypoxia *** to a low oxygen environment at a simulate-altitude of 3km for 8 weeks have caused the pathological remodeling of pulmonary vascular walls and pulmonary hypertension,and further led to a series of pathological changes,including right ventricular ***