Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly

作     者：Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao Xu Liu;Leilei Xu;Junying Li;Phil Y.Yao;Wanjuan Wang;Hazrat Ismail;Haowei Wang;Bryce Liao;Zhihong Yang;Tarsha Ward;Ke Ruan;Jianchun Zhang;Quan Wu;Ping He;Xia Ding;Dongmei Wang;Chuanhai Fu;Zhen Dou;Feng Yan;Wenwen Wang;Xing Liu;Xuebiao Yao

作者机构：MOE Key Laboratory for Cellular Dynamics&Anhui Key Laboratory for Chemical BiologyCAS Center for Excellence in Molecular Cell ScienceHefei National Science Center for Physical Sciences at Microscale&University of Science and Technology of ChinaHefei 230027China Department of PhysiologyMorehouse School of MedicineAtlantaGA 30310USA School of Basic Medical SciencesBeijing University of Chinese MedicineBeijing 100029China Department of BiologyDuke University DurhamNC 27708USA Institute of ProteoGenomicsBeijing 100029China Harvard Medical SchoolBostonMA 02115USA 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2020年第12卷第8期

页      面：654-665页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This work was supported in part by the National NaturalScience Foundation of China(31430054,31320103904,31621002,31671405,31601097,91854203,91753000,and91853115) 'Strategic Priority Research Program'of the ChineseAcademy of Sciences(XDB19000000) the National Key Researchand Development Program of China(2017YFA0503600 and2016YFA-0100500) MOE Innovative Team project(IRT_17R102) and the US National Institutes of Health(CA164133,DK56292,and DK115812) 

主　　题：organoids.cell division central spindle CENP-E syntelin PRC1 

摘      要：Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase ***,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains *** the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric *** sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during *** real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability *** biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic *** inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the ***,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone *** findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle *** CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.