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Identification of genes related to Nasopharyngeal Carcinoma with the help of Pathway-based Networks

第九届全国氢能学术会议

作者： y.r.zhu~(1,2),y.g.tang~1,j.h.yan~(1,2)and h.h.yang~(1,2) ~1 central south university,china ~2hunan Institute of Science and Technology,china

SrZryO particles were prepared by co-precipitation and solid phase method with TiO sol containing nitrogen source *** particles were further deposited by in situ photoreduction method to prepare heterojunction composite *** obtained composite catalysts were characterized by XrD,UV-Vis absorption spectrum and Fluorescence spectrum *** photocatalytic hydrogen generation from oxalic acid

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来源： cnki会议评论

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 900-910页

作者： hui LI~1 Cai-Ping rEN~1* Xiao-jun TAN~2 Xu-yu yang~1 hong-Bo ZhANg~1 Wen ZhOU~1 Kai-Tai yAO~1 1 Cancer research Institute,Xiangya School of Medicine,central south university,Changsha 410078,china 2 reproductive and Stem Cell Engineering Institute,Xiangya School of Medicine,central south university,Changsha 410078,china Cancer research Institute Xiangya School of Medicine Central South UniversityChangsha 410078 China reproductive and Stem Cell Engineering Institute Xiangya School of Medicine Central South UniversityChangsha 410078 China

cDNA microarray is a powerful tool to analyze simultaneously the expression levels of tens ofthousands of *** with normal nasopharynx (NP) tissues,2210 genes were highly differentiallyexpressed in nasopharyngeal carcinoma (NPC) tissues detected by cDNA *** signal pathwayis widely used to describe the complex relationship between genes,a pathway-based network was con-structed to visualize the connection between the genes obtained from microarray data in this *** the targeted genes that may have more important influence on this gene network with statisticalmethods and found that some genes might have significant influence on this network,especially ras-relatednuclear protein (rAN),carboxyl ester lipase (CEL),v-rel reticuloendotheliosis viral oncogene homolog A(rELA) *** verify the results from pathway-based selection,reverse transcription-polymerase chainreaction (rT-PCr) and real-time rT-PCr were performed to detect the expression levels of rAN,CEL andrELA genes and it was found that the rAN and CEL genes were significantly up-regulated in more than 80%of NPC *** further elucidate the function of the rAN gene,rAN expression was specifically sup-pressed in a 5-8F NPC cell line by rNA interference (rNAi).As expected,the depletion of rAN couldeffectively block the proliferation of tumor ***,our study may open up a new way to analyze thevast microarray data.

关键词： nasopharyngeal carcinoma microarray real-time PCr rNA interference ras-related nuclear protein (rAN)

Polymorphism Analyses of hepatitis B Virus X gene in hepatocellular Carcinoma Patients from southern china

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 265-272页

作者： Ping'an zhu~(1,2*) Deming TAN~1 Zhongtian PENg~1 Fei LIU~1 Lin SONg 1 Department of Infectious Diseases,Xiangya hospital,central south university,Changsha 410008,china 2 The Seventh hospital of Shenzhen,Shenzhen 518081,china Department of Infectious Diseases Xiangya Hospital Central South UniversityChangsha 410008 China The Seventh hospital of ShenzhenShenzhen 518081 China

Chronic hepatitis B virus(hBV)infection is one of the major causes of hepatocellularcarcinoma(hCC),and the hBV X(hBx)gene plays a critical role in the molecular pathogenesis ofhBV-related *** have investigated whether there are particular hBx gene mutations associated withhCC in patients from southern *** hBx gene was examined in 51 paraffin-embedded tumor tissuesamples from patients with hCC and 25 serum samples from the hBV carrier by nested polymerase chainreaction(PCr),single-stranded conformational polymorphism and heteroduplex *** hBx geneswith potentially important mutations from tumor tissue samples were cloned,sequenced and aligned withthe published hBx gene *** genotypes in tumor tissue samples were analyzed by nested *** of hBx gene polymorphism showed that 31.3% of hBx gene fragments in tumor tissue sampleshad a special pattern.A common deletion at nt 382-400 of the hBx gene accompanied by 29 point mutationswas detected in four randomly selected tumor tissue samples with this pattern which caused a frame-shiftin the hBx open reading frame with a new stop codon at nt 1818,resulting in an hBx polypeptide chaintruncated at the C end in these *** the four randomly selected samples,three were hBV genotypeB,and one was not detected by our present *** another tumor tissue sample,amplification of thefull-length hBx gene yielded a shorter *** of this fragment revealed a 264 bp deletionbetween nt 1577 and 1840 of the hBV *** results suggest that hBx gene mutation occurs frequentlyin hCC samples,and the deletion at nt 382-400 of the hBx gene might play a role in carcinogenesis of hCCin southern china.

关键词： hepatocellular carcinoma hepatitis B virus hBx polymorphism mutation

Landscape ecological assessment and eco-tourism development in the south Dongting Lake Wetland, china

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journal of Environmental Sciences 2003年第2期15卷 271-278页

作者： hE Ping 1,2, WANg Bao-zhong 1,3 (1.central south Forestry university, zhuzhou 412006, china 2.china International Engineering Consulting Corporation, Beijing 100044, china 3.Nanjing university, Nanjing 210093, china central south Forestry university Zhuzhou 412006

As an important resource and the living environment of mankind, wetland has become gradually a highlight, strongly concerned and intensively studied by scientists and sociologists. The governments in the world and the whole society have been paying more and more attention on it. The Dongting Lake of china is regarded as an internationally important wetland. For a rational development and protection of the wetland, an investigation and studied on its resources and its value to tourism in the south Dongting Lake was conducted, to create an assessment system of the ecological landscapes, and to evaluate qualitatively and quantitatively the value of wetland landscape to the eco-tourism. The results showed that the scenic value of the south Dongting Lake Wetland satisfied the criterion of AAAA grade of china national scenic attraction. The eco-tourism value of the landscape cultures in the south Dongting Lake Wetland was discussed with emphasis. It were formulated that a principle and frame of sustainable exploitation of the wetland landscapes and it was proposed as well that establishing a Wetland Park and developing eco-tourism in the south Dongting Lake. Wetland is a fragile ecosystem with low resistance to the impact of the exploitation. Thus, we must pay intensively attention to the influence of exploitation on the landscape, take the ecological risk in account to employ a right countermeasure and avoid the negative affection.

关键词： south Dongting Lake wetland landscape eco-tourism Wetland Park ecological assessment

Small Interfering rNA-mediated Caveolin-1 Knockout on Plasminogen Activator Inhibitor-1 Expression in Insulin-stimulated human Vascular Endothelial Cells

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 224-233页

作者： huiling yang~(1,3#) Shuya hE~(2,3#) Zhihua QUAN Weixia PENg~1 Bin yan~1 jianghua LIU~1 Fang WEN~1 renxian CAO~1 yangyan XU~3 gebo WEN~1 Weixin hU~2 1 Institute of Clinic Medicine,the First Affiliated hospital of Nanhua university,hengyang 421001,china 2 research Institute of Biochemistry and Molecular Biology.central south university Changsha 421001,china 3 Institute of Pharmacology and Pharmacy,Nanhua university,hengyang 421001,china Institute of Clinic Medicine the First Affiliated Hospital of Nanhua UniversityHengyang 421001 China Institute of Pharmacology and Pharmacy Nanhua UniversityHengyang 421001 China research Institute of Biochemistry and Molecular Biology Central South UniversityChangsha 421001 China

Using human vascular endothelial cells（ECV304）as the target,we studied the effect ofcaveolin（CAV）-1 in the course of insulin-stimulated expression of plasminogen activator inhibitor（PAl）-*** appropriate single-stranded oligonucleotides representing the rNAi CAV- 1 gene were analyzed by *** annealing to generate double-stranded oligonucleotides（ds oligo）,it was cloned into thepENTr/U6 entry vector containing rNA polymerase Ⅲ expression element by T4 DNA *** shorthairpin（shrNA）sequences transferred from the pENTr/U6 entry were cloned into the pLenti6/BLOCK-iT-DEST vector with an Lr recombination *** identification by sequencing,we successfully con-structed the CAV-1 rNAi lentiviral expression system using gateway *** efficiency wasassayed by real-time reverse transcription-polymerase chain reaction,immunofluorescence staining and ***304 cells were cultured in the medium containing different concentrations of insulin(1×10-9to1×10-7M)with the CAV-1 gene silenced or *** expression level and subcellular localization of PAI-1 andCAV-1 were compared using reverse transcription-polymerase chain reaction,immunofluorescence stainingand Western blot *** results showed that the potent inhibition of CAV-1 expression could reach 85%,and it was specific to the CAV-1-derived shrNA,not the S 100A13-derived *** was no dramaticdifference in PAI-1 expression between the rNAi+and rNAi-ECV304 cells incubated with physiologicalinsulin,but PAI-1 protein did accumulate under the cell *** the concentration of insulin increased,the expression of PAI-1 was up-regulated,whereas the expression of CAV-1 ***,PAI-1 clearly augmented after CAV-1 *** results indicated that hyperinsulinism could promotePAI-1 expression by inhibiting CAV-1,and stabilizing or up-regulating CAV-1 expression in endothelial cellsmight reduce complications of the great vessels and capillary vessels i

关键词： caveolin-1 plasminogen activator inhibitor-1 gene silencing insulin

Influence of Mg5ga2 compound on microstructures and electrochemical properties of Mg-5%hg-22%ga alloy

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Progress in Natural Science:Materials International 2011年第1期21卷 73-79页

作者： yan FENg~1,ri-chu WANg~1,Chao-qun PENg~1,hui-ping tang~2,hai-yan LIU~2 1.School of Materials Science and Engineering,central south university,Changsha 410083,china 2.Northwest Institute for Non-ferrous Metals research,Xi'an 710016,china School of Materials Science and Engineering Central South University Changsha 410083 China Northwest Institute for Non-ferrous Metals research Xi'an 710016 China

The Mg-5%hg-22%ga（mole fraction） alloy was *** at 573-673 K for different times was carried out to ensure the fine dispersion of the *** electron microscopy（SEM）,X-ray diffractometry（XrD） and energy spectrum analysis（ESA） were employed to characterize the microstructures and phases of the Mg-5%hg-22%ga *** electrochemical and corrosion behaviors of the above alloy were studied by potentiodynamic,galvanostatic and electrochemical impedance spectroscopy（EIS） *** relationship between the Mg5ga2compound and the electrochemical properties of the Mg-5%hg-22%ga alloy was *** results demonstrate that the heat treatment at 573-673 K for 24 h accelerates the growth of the intergranular eutectic Mg-Mg5ga2and the heat treatment for 200 h prompts the transition of eutectic to dispersed linear Mg phase and *** transition enhances the electrochemical activity and corrosion resistance of the Mg-5%hg-22%ga *** the galvanostatic tests with current density of 100 mA/cm2,the most negative potential,-1.991 V, occurs in the sample aged for 200 *** largest corrosion current density of 44.36 mA/cm2occurs in the sample aged for 24 h.

关键词： Mg-hg-ga alloys anodic behavior Mg5ga2 phase intergranular corrosion

Preparation of insoluble β-CD polymer and its application to enantiomers and isomers separation

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journal of central south university of Technology 2001年第1期8卷 57-59页

作者： yI jian ming 1,tang Kuo wen 2 (1.College of Chemistry and Chemical Engineering, central south university, Changsha 410083, china 2.Department of Chemical Engineering, yueyang Normal university, yueyang 414000, china) College of Chemistry and Chemical Engineering Central South University Changsha China Department of Chemical Engineering Yueyang Normal University Yueyang China

An insoluble β-cyclodextrin polymer cross-linked with epichlorohydrin was prepared, and its structure was identified with infrared spectrum. Colloid stationary phase was prepared by dissolving the polymer in the mixed solvent of diisopropyl ether, methylene dichloride and benzene and treated for 0.5 h by ultrasonication, and then was coated on a fused silica capillary column. The optimun reaction conditions are as follows: the mole ratio of epichlohydrin to β-cyclodextrin is 12.1:1, reacting at 65 °C for 24 h. The Chromatographic performance such as column efficiency, thermal stabilities and polarity were studied, two kinds of disubstituted benzene isomers and eight pairs of enantiomers were separated on the capillary column. The results show that the β-cyclodextrin polymer is suitable for use as a capillary gas chromatographic stationary phase, and possess excellent chromatographic properties in separating enantiomers and position isomers.

关键词： insoluble β cyclodextrin polymer capillary gas chromatography enantiomer position isomer separation

Simulation of moving boundary of the reaction shaft in a flash smelting furnace

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journal of central south university 2001年第3期13卷 213-218页

作者： ChEN zhuo 1,MEI Chi 1,ChEN hong-rong 1,MO jiao 2 (1 Institute on Simulation and Optimization of Pyro-installation, central Sout h university, Changsha 410083, china 2 Science School, Beijing university of Posts and Telecommunications, Beijing 1 Institute on Simulation and Optimization of Pyro-installation Central South University Changsha China Science School Beijing University of Posts and Telecommunications Beijing China

As to solve the online monitoring of the inner temperatur e and freezing profile of the reaction shaft of flash smelting furnace, simulation of the wall in the reaction shaft in a flash smelting furnace was made through numerical computation. The computational results are very near the data got in s ite. The error of the moving boundary is approximately 3%, and that of the tempe rature is less than 5%. It is proved that the simulation software is applicable for practice to monitor the temperature and moving boundary inside the hearth on line. Based on a large number of the data computed, the relation between the cha nge of the moving boundary and inner temperature is summarized, and the great in fluence of the cooling system on the forming and stability of the moving boundar y inside the hearth is emphasized, which provide the theoretical bases for optim izing the flash smelting operation.

关键词： flash smelting furnace moving boundary numerical computation simulation

rECOVEry OF CADMIUM AND NICKEL FrOM SCrAP Ni-Cd BATTErIES

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Acta Metallurgica Sinica(English Letters) 2001年第4期14卷 303-312页

作者： j.X. zhu1), B. yu2), j.h. Li1) and y.F. Nie1) 1) Department of Environmental Engineering, Tsinghua university, Beijing 100084, china 2) Institute of Nuclear Energy Technology, Tsinghua university, Beijing 100084, china Dept. of Environ. Eng. Tsinghua Univ. Beijing 100084 China

Several typical methods for the recovery of Ni-Cd batteries are described in detail. Based on the comparison between the hydrometallurgical process and the pyrometallurgical process, the latter is selected as the suit... 详细信息

关键词： Cadmium Nickel recovery Scrap metal

Biocompatibility evaluation in vitro. Part III: Cytotoxicity expression of human and animal osteoblasts on the biomaterials

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journal of central south university 2001年第3期13卷 147-155页

作者： rUAN jian-ming 1,M.h. grant 2 (1. State Key Laboratory for Powder Metallurgy, central south university, Changs ha 410083, china 2. Bioengineering Unit, Strathclyde university, glasgow, g4 0NW, UK) State Key Laboratory for Powder Metallurgy Central South University Changsha China Bioengineering Unit Strathclyde University Glasgow UK

Exclusion and inflammation in the clinic are observe d for various reas ons including material che- mical composition, physical properties as well a s macr o- and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue i n current biocompatibility to screen the potential implant materials and drugs. This study was aimed at investigation reaction between the potential implant mat erials and surround tissue. Cytotoxicity of human and rat osteoblast in the mate rial extracts was determinated by testing standards such as ghS assay, MTT assay , alkaline phosphatase activity assay, LDh assay, and Lowry assay. research resu lts demonstrated that compared with the control condition polystyrene culture pl a te both human and rat osteoblast cells have normal phenotypic expression in hydr oxyapatite extract, and this expression was statistically restricted in hydroxya patite-spinel extract. how- ever, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.

关键词： biomaterial cytotoxicity biological molecular technique