Polymorphism Analyses of Hepatitis B Virus X Gene in Hepatocellular Carcinoma Patients from Southern China
Polymorphism Analyses of Hepatitis B Virus X Gene in Hepatocellular Carcinoma Patients from Southern China作者机构：Department of Infectious Diseases Xiangya Hospital Central South UniversityChangsha 410008 China The Seventh Hospital of ShenzhenShenzhen 518081 China
出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))
年 卷 期：2007年第39卷第4期
页 面：265-272页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：hepatocellular carcinoma hepatitis B virus HBx polymorphism mutation
摘 要：Chronic hepatitis B virus(HBV)infection is one of the major causes of hepatocellularcarcinoma(HCC),and the HBV X(HBx)gene plays a critical role in the molecular pathogenesis ofHBV-related *** have investigated whether there are particular HBx gene mutations associated withHCC in patients from southern *** HBx gene was examined in 51 paraffin-embedded tumor tissuesamples from patients with HCC and 25 serum samples from the HBV carrier by nested polymerase chainreaction(PCR),single-stranded conformational polymorphism and heteroduplex *** HBx geneswith potentially important mutations from tumor tissue samples were cloned,sequenced and aligned withthe published HBx gene *** genotypes in tumor tissue samples were analyzed by nested *** of HBx gene polymorphism showed that 31.3% of HBx gene fragments in tumor tissue sampleshad a special pattern.A common deletion at nt 382-400 of the HBx gene accompanied by 29 point mutationswas detected in four randomly selected tumor tissue samples with this pattern which caused a frame-shiftin the HBx open reading frame with a new stop codon at nt 1818,resulting in an HBx polypeptide chaintruncated at the C end in these *** the four randomly selected samples,three were HBV genotypeB,and one was not detected by our present *** another tumor tissue sample,amplification of thefull-length HBx gene yielded a shorter *** of this fragment revealed a 264 bp deletionbetween nt 1577 and 1840 of the HBV *** results suggest that HBx gene mutation occurs frequentlyin HCC samples,and the deletion at nt 382-400 of the HBx gene might play a role in carcinogenesis of HCCin southern China.
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