Identification of Genes Related to Nasopharyngeal Carcinoma with the Help of Pathway-based Networks

作     者：Hui LI~1 Cai-Ping REN~1* Xiao-Jun TAN~2 Xu-Yu YANG~1 Hong-Bo ZHANG~1 Wen ZHOU~1 Kai-Tai YAO~1 1 Cancer Research Institute,Xiangya School of Medicine,Central South University,Changsha 410078,China 2 Reproductive and Stem Cell Engineering Institute,Xiangya School of Medicine,Central South University,Changsha 410078,China 

作者机构：Cancer Research Institute Xiangya School of Medicine Central South UniversityChangsha 410078 China Reproductive and Stem Cell Engineering Institute Xiangya School of Medicine Central South UniversityChangsha 410078 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2006年第38卷第12期

页      面：900-910页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by a grant from the Hunan Science and Technology Fund 

主　　题：nasopharyngeal carcinoma microarray real-time PCR RNA interference Ras-related nuclear protein (RAN) 

摘      要：cDNA microarray is a powerful tool to analyze simultaneously the expression levels of tens ofthousands of *** with normal nasopharynx (NP) tissues,2210 genes were highly differentiallyexpressed in nasopharyngeal carcinoma (NPC) tissues detected by cDNA *** signal pathwayis widely used to describe the complex relationship between genes,a pathway-based network was con-structed to visualize the connection between the genes obtained from microarray data in this *** the targeted genes that may have more important influence on this gene network with statisticalmethods and found that some genes might have significant influence on this network,especially Ras-relatednuclear protein (RAN),carboxyl ester lipase (CEL),v-rel reticuloendotheliosis viral oncogene homolog A(RELA) *** verify the results from pathway-based selection,reverse transcription-polymerase chainreaction (RT-PCR) and real-time RT-PCR were performed to detect the expression levels of RAN,CEL andRELA genes and it was found that the RAN and CEL genes were significantly up-regulated in more than 80%of NPC *** further elucidate the function of the RAN gene,RAN expression was specifically sup-pressed in a 5-8F NPC cell line by RNA interference (RNAi).As expected,the depletion of RAN couldeffectively block the proliferation of tumor ***,our study may open up a new way to analyze thevast microarray data.