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Asian Journal of Pharmaceutical Sciences 2018年第3期13卷 229-238页

作者： xiuping zhang Yang Shi Zhiyue zhang Zhenlei Yang Guihua Huang Department of Pharmaceutical Science Shandong University

Tetramethylpyrazine(TMP) is a traditional Chinese herbal medicine with strong antiinflammatory and cartilage protection activities, and thus a promising candidate for treating osteoarthritis. However, TMP is rapidly cleared from the joint cavity after intra-articular injection and requires multiple injections to maintain efficacy. The aim of this study was to encapsulate TMP into poly(lactic-co-glycolic acid)(PLGA) microspheres to enhance the TMP retention in the joint, reducing injection frequencies and decreasing dosage. TMP microspheres were prepared by emulsion/solvent evaporation method. The intra-articular retention of the drug was assessed by detecting the drug concentration distributed in the joint tissue at different time points. The therapeutic effect of TMP microspheres was evaluated by the swelling of knee joints and histologic analysis in papain-induced OA rat model. The prepared freezedried microspheres with a particle size of about 10 μm can effectively prolong the retention time of the drug in the articular cavity to 30 d, which is 4.7 times that of the TMP ***-articular injection of TMP microspheres efficiently relieved inflammatory symptoms,improved joint lesions and decreased the depletion of proteoglycan. In conclusion, intraarticular injection of TMP loaded microspheres was a promising therapeutic method in the treatment of OA.

关键词： Osteoarthritis Tetramethylpyrazine Intra-articular injection PLGA microspheres Retention Pharmacodynamics

Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

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Neural Regeneration Research 2012年第12期7卷 917-923页

作者： Fengguo Zhai xiuping zhang Yue Guan Xudong Yang Yang Li Gaochen Song Lixin Guan Department of Pharmacology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Endocrinology Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Thoracic Surgery Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China Department of Biochemistry Mudanjiang Medical University Mudanjiang 157011 Heilongjiang Province China

Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.

关键词： focal cerebral ischemia microRNA apoptosis bcl-2 caspase-3 neural regeneration

Exploration of the potential mechanism of Danggui Shaoyao powder in the treatment of endometriosis based on bioinformatics

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Journal of Traditional Chinese Medical Sciences 2019年第4期6卷 355-364页

作者： xiuping zhang Lulu zhang Qi Wang Xinhui Sun Yang Dong Yu Xing Xiaona Ma School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Department of Gynecology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China Harvard Medical School Brigham and Women’s Hospital02115 BostonMAUSA

Background:Endometriosis is a hormone-and organ-dependent disease with unclear *** Shaoyao powder(DSP)has been used for the treatment of endometriosis for many years and has been proven to be effective,but its underlying molecular mechanisms remain *** this study,we explored the molecular targets and pathways of DSP in the treatment of endometriosis mainly from the perspective of network pharmacology,and provided some novel ideas for managing this ***:In the present study,we focused on the underlying mechanisms between DPS and endometriosis,via oral bioavailability screening,drug similarity assessment,target identification,network analysis,drug relocation,and molecular docking using network ***:By bioinformatics,54 active components of DSP were predicted from the 534 components provided by the TCMSP *** then found 85 validated targets and 6059 predicted targets for herbal components,and 2241 targets for *** mapping,there were 255 targets in *** that,network analysis was performed to screen key networks and core *** indirect targets were collected from 14 direct *** and KEGG analyses identified 63 enriched functions and 10 pathways(P<.01).According to the disease target,dipyridamole could undergo drug repositioning for endometriosis(score?1).The mechanism of action of dipyridamole on endometriosis was compared with that of ***,eight targets,namely,AR,ESR1,HMGCR,NOS2,NR3C1,PPARG,PTGES,and PTGS2,were validated by molecular docking,suggesting that the probable molecular mechanisms by which DSP treats endometriosis are through seven validated pathways:hsa05200,hsa04915,hsa01100,hsa00900,hsa04920,hsa01130,and ***:In this study,we uncovered the mechanism by which DSP can treat endometriosis using a drugecomponentetarget interaction *** approach can provide some novel ideas for the development of Chinese medicine and the clinical managem

关键词： Danggui shaoyao powder Endometriosis Network pharmacology Molecular docking Mechanism

微波花椒鸡丁预制菜的工艺优化及其挥发性风味物质分析

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四川旅游学院学报 2025年第1期 30-35页

作者：刘明珠华玲张秀萍丁燃容刘媛媛四川旅游学院四川成都610100

为优化微波花椒鸡丁预制菜的加工工艺,本研究在单因素试验基础上,以感官评分为指标,对微波花椒鸡丁的加工工艺进行响应面试验设计以确定其最佳加工工艺,并采用气相色谱—离子迁移谱法(GC-IMS)分析微波花椒鸡丁的挥发性风味物质。结果表... 详细信息

为优化微波花椒鸡丁预制菜的加工工艺,本研究在单因素试验基础上,以感官评分为指标,对微波花椒鸡丁的加工工艺进行响应面试验设计以确定其最佳加工工艺,并采用气相色谱—离子迁移谱法(GC-IMS)分析微波花椒鸡丁的挥发性风味物质。结果表明:当花椒添加量5.0g,水淀粉添加量18.0g,微波加热时间7.50min,辣椒粉添加量5.0g,微波功率1000W时,微波花椒鸡丁色香味最佳。GC-IMS共鉴定出71种挥发性风味物质,其中萜烯类和酯类的种类繁多、含量丰富,是微波花椒鸡丁风味的主要贡献者。

关键词：花椒鸡丁微波工艺响应面法挥发性风味物质

Hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis

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Journal of Traditional Chinese Medical Sciences 2023年第2期10卷 179-185页

作者： Yuejian zhang Changxiang Li Conglu Sui xiuping zhang Ya'nan Guo Tiantian He Taoxiu Lin Xiaona Ma The Third School of Clinical Medicine Beijing University of Chinese MedicineBeijing100029China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Department of Gynecology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China

Objective:To explore the effect of Hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,*** identified their stem cells’characteristics via adipogenic and osteogenic *** male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,*** used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best *** cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP *** analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western ***:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic *** then used the best conditiond 20% serum and 48 hdfor the subsequent *** contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(P<0.001 and P=0.001,respectively),and higher expression of LC3-II and Beclin1(P=0.021 and P=0.019,respectively).Conclusion:Hupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects.

关键词： Hupo powder Endometriosis Menstrual blood-derived stem cells Autophagy Beclin1 LC3-II

Potential mechanism of Huatan Qushi decoction on improving phlegm-dampness constitution using microRNA array and RT-qPCR targeting on hsa-miR-1237e3p

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Journal of Traditional Chinese Medical Sciences 2021年第1期8卷 43-51页

作者： xiuping zhang Ji Wang Baoxing Liu Haiqiang Yao Yu Chen Yuqing Yin Xuejing Yang Lingru Li Department of Gynecology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China Center for Studies in Constitution Research of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 100029China Department of Andrology China-Japan Friendship HospitalBeijing 100029China Department of Nutriology China-Japan Friendship HospitalBeijing 100029China Memorial Sloan Kettering Cancer Center New YorkNY10065USA

Background:Metabolic diseases pose considerable burden on the healthcare system worldwide,indicating the significance of prevention and *** constitution theory of traditional Chinese medicine,phlegm-dampness constitution(PDC)is the common basis of metabolic *** clinical practice,Huatan Qushi(HTQS)decoction targeting on PDC can effectively improve metabolic ***,its underlying biochemical mechanism still remains ***:Eight PDC participants received HTQS decoction for three *** blood was collected at baseline and 1 and 3 months after intervention *** biomedical indicators were ***-throughput sequencing and RT-qPCR were used for *** to the missing data,repeated measures with missing values in mixed models were ***:After 3-month treatment,HDL-C level increased(P<.001)and FBG,FINS,and HbA1c all showed decreasing trend at different time points(all P<.05).After miRNA high-throughput sequencing,compared with the baseline,differential miRNAs at 1 and 3 months were screened,and target gene prediction and KEGG pathway enrichment analysis were *** results displayed that metabolic disease-related pathways mainly included pathways in cancer,PI3K-Akt signaling pathway,***,RT-qPCR showed that hsa-miR-1237e3p differed statistically(P=.008).Then we validated the target genes of hsa-miR-1237e3p in the“Pathways in Cancer”pathway including SDF1,AC,CRK,and HGF,also known as upstream target genes of PI3K/AKT *** results showed that two indicators of CRK and HGF were in statistical significance(P=.045 and P=.036,respectively).Conclusion:PDC serves as a common basis for various metabolic *** adjusting PDC,HTQS decoction can improve biomedical indicators including blood glucose,HbA1c,insulin,and *** target pathway is“Pathways in cancer”.Specifically,HTQS decoction acts on targets of CRK and HGF by regulating hsa-miR-1237e3p,and probably exerts effects on t

关键词： Chinese herbal formula Constitution Metabolic disorders Pathways in cancer

Huatan Qushi formula alleviates non-alcoholic fatty liver disease via PI3K/Akt signaling and gut microbiota modulation

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Journal of Traditional Chinese Medical Sciences 2024年第4期11卷 443-455页

作者： xiuping zhang Linghui Zhu Jinchen Ma Yi Zheng Xuejing Yang Lingling Yang Yang Dong Yan zhang Baoxing Liu Lingru Li Department of Gynecology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China Memorial Sloan Kettering Cancer Center 1275 York AvenueNew YorkNY 10065USA Department of Hepatobiliary Spleenand Stomach DiseasesHenan Province Hospital of TCMZhengzhou450002China Monitoring and Statistical Research Center National Administration of Traditional Chinese MedicineBeijing100027China National Institute of Traditional Chinese Medicine Constitution and Preventive Treatment of Diseases Beijing University of Chinese MedicineBeijing100029China Department of Andrology China-Japan Friendship HospitalBeijing100029China a r t i c l e i n f o Article history:Received 21 May 2024 Received in revised form 29 August 2024 Accepted 31 August 2024 Available online 6 September 2024 Keywords:Non-alcoholic fatty liver disease Huatan Qushi formula Network pharmacology PI3K/Akt signaling pathway 16S rRNA sequencing Gut microbiota

Objective:To provide the mechanism-based pharmacotherapy of the Huatan Qushi formula(HTQS for-mula),for the health management and treatment of non-alcoholic fatty liver disease(NAFLD).Methods:A rat model of NAFLD was employed to examine the efficacy and safety of the HTQS *** vivo active components and potential mechanisms of the HTQS formula were identified using UPLC‒MS/MS combined with network *** influence of the HTQS formula on the dominating proteins in PI3K/Akt pathway was validated in vivo using western ***,16S rRNA sequencing of the gut microbiome was conducted followed by targeted metabolomics detecting fecal short-chain fatty acids(SCFAs)and bile acids to determine the impact of the HTQS formula on gut ***:The HTQS formula reduced weight gain and hepatic steatosis in NAFLD rats and decreased serum total cholesterol(TC),triglycerides,blood glucose,and insulin resistance(IR)without causing liver or kidney *** detected 28 components using UPLC‒MS/MS and identified 439 shared targets be-tween NAFLD and the HTQS ***,we focused on the PI3K/Akt signaling pathway based on protein‒protein interaction network *** validated that the HTQS formula inhibited liver stea-tosis and inflammation by increasing the phosphorylation levels of PI3K,AKT,P27,GSK3b in the PI3K/Akt signaling pathway.16S rRNA sequencing revealed that the HTQS formula reduced the abundance of the genus Family_XIII_AD3011_group,which was positively correlated with IR and taurodeoxycholic *** addition,Lachnospiraceae_UCG_010 inversely correlated with TC and five bile acids,which could be essential to the therapeutic effect of the HTQS formula against ***:The HTQS formula proved to be an effective pharmacotherapy for NAFLD without causing liver or kidney *** potent components of the HTQS formula could alleviate liver steatosis and lipid metabolism disorder by modulating the PI3K/Akt signaling pathway an

关键词： Non-alcoholic fatty liver disease Huatan Qushi formula Network pharmacology PI3K/Akt signaling pathway 16S rRNA sequencing Gut microbiota

The Study on the Sharing Information in Supply Chains

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Chinese Business Review 2005年第7期4卷 31-34页

作者： xiuping zhang Dong Li Minjie Zhao Ju zhang Central University for Nationalities Peking University China Life Insurance Company/Renmin University of China Inner Mongolia Finance and Economics College

In this paper, we discuss the relationship between the sharing of information of supply chains and the supply chain management; we also discuss the function of information share, and the obstacles in sharing information. As we all know, information share between enterprises in supply chains is necessary and possible, but the existence of obstacles of sharing information in supply chains is also a severe challenge in practice. This paper tries to find right ways for enterprises to build the real trade partners＇ relationship and realize transferring the information smoothly in all these three levels among the partners in supply chains.

关键词： share information supply chain

Ligands induce the mutual movements between the HP1 and TM4 of the glutamate transporter EAAT1

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Ligands induce the mutual movements between the HP1 and TM4 ...

生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： Shaogang Qu xiuping zhang Department of Immunology Southern Medical University Teaching Center of Experimental Medicine Southern Medical University

Excitatory amino acid transporter 1(EAAT1) plays an importance role in uptaking glutam-ate from the synaptic cleft and keeping the extracellular glutamate concentration under the neurotoxic *** reentrant helical hairpin(HP) 1 is part of the intracellular gate of the *** of this intracellular gate,the transporter releases sodium and substrate to the ***

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公允价值计量与会计目标的理念分歧

江淮论坛 2011年第3期 47-53页

作者：张修平中国人民大学商学院北京100872

公允价值计量属性自引入会计准则以来一直是会计界的热点问题。本文从公允价值的起源、发展和引入会计准则的目标开始,分析其问题所在,旨在得出这样的结论:公允价值计量属性不仅与会计目标存在事实上的分歧,不能实现其引入初衷,而且在... 详细信息

公允价值计量属性自引入会计准则以来一直是会计界的热点问题。本文从公允价值的起源、发展和引入会计准则的目标开始,分析其问题所在,旨在得出这样的结论:公允价值计量属性不仅与会计目标存在事实上的分歧,不能实现其引入初衷,而且在一定程度上损害了会计的传统职能。本文对此给出了一些针对性的解决思路。

关键词：公允价值财务报表税法企业内在价值管理活动论