Hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis

作     者：Yuejian Zhang Changxiang Li Conglu Sui Xiuping Zhang Ya'nan Guo Tiantian He Taoxiu Lin Xiaona Ma Yuejian Zhang;Changxiang Li;Conglu Sui;Xiuping Zhang;Ya'nan Guo;Tiantian He;Taoxiu Lin;Xiaona Ma

作者机构：The Third School of Clinical MedicineBeijing University of Chinese MedicineBeijing100029China School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing102488China Department of GynecologyBeijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2023年第10卷第2期

页      面：179-185页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study was supported by the National Natural Science Foundation of China(81973895) the Key Research Projects of Beijing University of Chinese Medicine(2020-JYB-ZDGG-143-3) 

主　　题：Hupo powder Endometriosis Menstrual blood-derived stem cells Autophagy Beclin1 LC3-II 

摘      要：Objective:To explore the effect of Hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,*** identified their stem cells’characteristics via adipogenic and osteogenic *** male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,*** used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best *** cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP *** analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western ***:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic *** then used the best conditiond 20% serum and 48 hdfor the subsequent *** contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(P0.001 and P=0.001,respectively),and higher expression of LC3-II and Beclin1(P=0.021 and P=0.019,respectively).Conclusion:Hupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects.