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Towards understanding the correlation between UO_2^(2+) extraction and substitute groups in 2,9-diamide-1,10-phenanthroline

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Science China Chemistry 2018年第10期61卷 1285-1292页

作者： xinrui zhang Liyong Yuan Zhifang Chai Weiqun Shi Laboratory of Nuclear Energy Chemistry Institute of High Energy Physics Chinese Academy of Sciences School for Radiological and Interdisciplinary Sciences (RAD-X) and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University

2,9-Diamide-1,10-phenanthroline(DAPhen)ligands represent a new family of tetradentate extractants given their strong affinity to actinides and the CHON *** this family,N,N′-diethyl-N,N′-ditolyl-2,9-diamide-1,10-phenanthroline(Et-TolDAPhen),initially reported by us,exhibits excellent selectivity towards actinides(U,Th,Am,Pu)over lanthanides and thus can be potentially applied in the group actinide extraction(GANEX)process for the group separation of *** this article,by tailoring the lengths of alkyl chains,we synthesized other four DAPhen ligands with different substitute groups in the diamide moieties,and characterized the relationship between properties and substitute groups of DAPhen *** extraction results show that three of the ligands exhibit high performance in UO_2^(2+)extraction from an acidic solution and the extracted UO_2^(2+)can be easily stripped by only using ultrapure *** titration confirms that UO_2^(2+)combined with all the four ligands in 1:1 *** extended X-ray absorption finestructure(EXAFS)study shows that six donor atoms comprise the first equatorial shell of the UO_2^(2+)ions bonded by the DAPhen ligands,among which two nitrogen and two oxygen atoms are from the DAPhen ligand,while other two oxygen atoms are from one nitrate *** article promises to provide basic data for assessing the feasibility of this kind of DAPhen ligands applied in actinides separation from nuclear wastes.

关键词： 2,9-diamide-1,10-phenanthroline UO2^2+ extraction UV-Vis titration EXAFS

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Study on enzymatic hydrolysis of soybean β-conglycinin using alkaline protease from Bacillus subtilis ACCC 01746 and antigenicity of its hydrolysates

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Grain & Oil Science and Technology 2021年第1期4卷 18-25页

作者： Haicheng Yin xinrui zhang Jin Huang College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

Due to its beneficial health effects,the use of soybean protein has shown a continuous increase,but concerns regarding the allergenicity of soybean antigenic protein have also *** study aimed to evaluate the hydrolytic effects of a non-commercial alkaline protease isolated from the Bacillus subtilis ACCC 01746 on soybeanβ-conglycinin and the allergenicity of its *** protease of the strain was separated by precipitation method of organic solvents,and theβ-conglycinin was separated by alkali-solution and acid-isolation and purified by use of gel *** the degree of hydrolysis(DH)and inhibition rate as evaluation indexes,the enzymatic hydrolysis parameters ofβ-conglycinin was optimized by single factor and L_(9)(3^(4))orthogonal tests,so as to explore the effect of the protease on the hydrolysis degree and the antigenicity ofβ-conglycinin *** results showed that the native enzyme existed as an 18.3 kDa monomer with a 430 U/g maximum *** purity ofβ-conglycinin was 84.8%.The single-factor test results showed that DH showed the oppostie trendency with the inhibition rate,and the increase of protein concentration causedmonotone increasing and monotone decreasing of the inhibition rate and the DH,and the optimal protein concentration was 30 mg/*** optimization results showed that pH had the largest impacts on both DH and the inhibition rate,followed by enzyme dosage,hydrolysis temperature and hydrolysis *** the optimum hydrolysis conditions of protein concentration 30mg/mL,enzymedosage0.7%,hydrolysis time40min,temperature 55°C and pH8.5,the DH reached the highest of 76.28%,and the inhibition rate was the lowest of 27.03%,which was reduced greatly compared with that before *** results suggested that alkaline protease appeared to show a relatively high effeciency in lowering soybean allergenicity,making it possible to produce low-allergenicity soybean protein.

关键词： Alkaline protease Bacillus subtilis ACCC 01746 β-conglycinin Hydrolysis degree Antigenicity

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Hypoxia-mediated programmed cell death is involved in the formation of wooden breast in broilers

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1918-1931页

作者： xinrui zhang Tong Xing Lin zhang Liang Zhao Feng Gao College of Animal Science and Technology Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu ProvinceJiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety ControlNanjing Agricultural UniversityNo.1 WeigangNanjingJiangsu 210095P.R.China

Background Wooden breast(WB)myopathy is a common myopathy found in commercial broiler chickens *** examination has revealed that WB myopathy is accompanied by damage to the pectoralis major(PM)***,the underlying mechanisms responsible for the formation of WB in broilers have not been fully *** study aimed to investigate the potential role of hypoxia-mediated programmed cell death(PCD)in the formation of WB *** Histological examination and biochemical analysis were performed on the PM muscle of the control(CON)and WB groups.A significantly increased thickness of the breast muscle in the top,middle,and bottom portions(P<0.01)was found along with pathological structure damage of myofibers in the WB *** number of capillaries per fiber in PM muscle,and the levels of p O_(2) and s O_(2) in the blood,were significantly decreased(P<0.01),while the levels of p CO_(2) and TCO_(2) in the blood were significantly increased(P<0.05),suggesting hypoxic conditions in the PM muscle of the WB *** further evaluated the PCD-related pathways including autophagy,apoptosis,and necroptosis to understand the consequence response to enhanced hypoxic conditions in the PM muscle of birds with *** ratio of LC3 II to LC3 I,and the autophagy-related factors HIF-1α,BNIP3,Beclin1,AMPKα,and ULK1 at the m RNA and protein levels,were all significantly upregulated(P<0.05),showing that autophagy occurred in the PM muscle of the WB *** apoptotic index,as well as the expressions of Bax,Cytc,caspase 9,and caspase 3,were significantly increased(P<0.05),whereas Bcl-2 was significantly decreased(P<0.05)in the WB-affected PM muscle,indicating the occurrence of apoptosis mediated by the mitochondrial ***,the expressions of necroptosis-related factors RIP1,RIP3,and MLKL,as well as NF-κB and the pro-inflammatory cytokines TNF-α,IL-1β,and IL-6,were all significantly enhanced(P<0.05)in the WB-affected PM *** T

关键词： Apoptosis Autophagy Broiler chicken Hypoxia Necroptosis Wooden breast

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Unveiling the protective role of biofilm formation on the photoaging of microplastics

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Science China(Technological Sciences) 2024年第10期67卷 3067-3078页

作者： zhang HaiYu LIU Peng zhang JiaYu DAI JiaMin zhang xinrui zhang ZiXuan JIA HanZhong Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China Ministry of AgricultureCollege of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100China Key Laboratory of Organic Compound Pollution Control Engineering School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China

In natural water,microplastics(MPs)inevitably undergo microbial colonization to form biofilm,while the effect of biofilm formation on the photoaging of MPs remains *** study systematically investigated the photoaging behavior of disposable box-derived polypropylene(PPMPs)and polystyrene(PSMPs)mediated by formed biofilm in *** incubating in Weihe water samples for 105 d,the biofilm was validated to form in PPMPs and PSMPs,with higher formation in *** particular,biofilm formation inhibited the photoaging of PPMPs and PSMPs,with an 11.1%and 50.6%decrease in photooxidation compared to their virgin counterparts after 20 d of ultraviolet(UV)***,the photoaging route of MPs such as the reaction priority of functional groups(e.g.,C–H,C=O,and benzene ring)was altered,indicating the important role of biofilm formation in the photoaging process of *** protective effect of the biofilm was mainly caused by the role of optical light filters that absorbed UV energy by sacrificing ***,as the main attacker on MPs,the production of reactive oxygen species(ROS,mainly^(1)O_(2)and·OH)was inhibited by biofilm,which was mainly responsible for the decreased photoaging of *** study revealed the important role of biofilm in the photoaging process of MPs and suggested a higher resistance of(micro)plastics in natural water than that in the laboratory using pure MPs,which contributed to a deeper understanding of the environmental fate and pollution of MPs.

关键词： microplastics biofilm photoaging physical shielding reactive oxygen species

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大鼠脂肪间充质干细胞及其外泌体的蛋白组学对比分析

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中国组织工程研究 2025年第13期29卷 2683-2689页

作者：张鑫瑞韩悦雷蕾刘建宇耿承奎云南中医药大学第二临床医学院云南中医药大学呈贡校区云南省昆明市650000 昆明医科大学附属延安医院骨科云南省昆明市650000

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外... 详细信息

背景:从蛋白组学方面分析脂肪间充质干细胞及其来源外泌体促进组织修复的研究较多,但缺少二者之间的蛋白组学对比分析。目的:对脂肪间充质干细胞及其外泌体进行蛋白组学分析。方法:分离培养大鼠脂肪间充质干细胞,从细胞上清液中提取外泌体并进行鉴定;运用DIA蛋白组学分析大鼠脂肪间充质干细胞及其外泌体差异表达蛋白,对差异表达蛋白进行GO和KEGG分析。结果与结论:①脂肪间充质干细胞以长梭形为主,类似成纤维样;②经BCA法测定外泌体悬液的蛋白质量浓度为7.66 mg/mL;③外泌体呈典型的茶杯型,可见双层膜的囊泡结构,中央为低电子密度成分,外泌体粒径分布峰值为112.2 nm,浓度为7.5×10^(11)粒子/mL,平均直径在70-140 nm之间;④外泌体有表面标记蛋白CD9、TSG101高表达;⑤GO分析脂肪间充质干细胞及其外泌体的差异表达蛋白主要集中在细胞外基质和突触等位置,与离子结合和核糖体的结合能力相关,在细胞黏附和翻译过程中最为显著;⑥KEGG通路分析结果显示,差异表达蛋白主要参与细胞外基质受体交互、核糖体和细胞因子受体相互作用等,并且与胆固醇和甲状腺等多种代谢性疾病有关。

关键词：脂肪间充质干细胞外泌体蛋白组学对比分析组织修复

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Growth of Single-walled Carbon Nanotubes on Substrates Using Carbon Monoxide as Carbon Source

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Chemical Research in Chinese Universities 2021年第5期37卷 1125-1129页

作者： ZHAO Xue zhang xinrui LIU Qidong zhang Zeyao LI Yan Beijing National Laboratory for Molecular Science Key Laboratory for the Physics and Chemistry of NanodevicesState Key Laboratory of Rare Earth Materials Chemistry and ApplicationsCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing100871P.R.China Peking University Shenzhen Institute Shenzhen518057P.R.China College of Engineering Peking UniversityBeijing100871P.R.China PKU-HKUST ShenZhen-HongKong Institution Shenzhen518057P.R.China

The growth of single-walled carbon nanotubes(SWCNTs)on substrates has attracted great interests because of the potential applications in various *** monoxide(CO)was used as the carbon source for the growth of SWCNTs on silicon *** or oriented SWCNTs can be produced by varying the CO flow *** the flow rate of CO was as low as 20 sccm(sccm:standard cubic centimeter per minute).

关键词： SWCNTs Carbon walled

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Enzymatic kinetics ofβ-conglycinin using alkaline protease from Bacillus subtilis ACCC 01746 and analysis of antigenicity of hydrolyzed peptide

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Grain & Oil Science and Technology 2021年第3期4卷 141-148页

作者： Haicheng Yin xinrui zhang Zhixiang Yang Jin Huang College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

β-Conglycinin,the main protein of soybean,is a key allergen that causes soybean allergies,and hydrolysis is usually applied to lower its *** evaluated the enzymolysis characters ofβ-conglycinin from the perspective of enzymolysis kinetics using alkaline protease from *** ACCC 01746.A dynamic model describing the hydrolysis ofβ-conglycinin was proposed using the initial substrate concentration,enzyme dosage(enzyme to substrate ratio)and hydrolysis time as variables to illustrate the kinetic behavior of enzymatic *** hydrolysis of soybeanβ-conglycinin was carried out at 60 g/L protein concentration,0.6%enzyme dosage,55℃ and pH 8.5 to observe the peptides with anti-enzymatic *** hydrolysates were gradually fractionated by ultrafiltration through cut-off membranes with molecular weights of 40,30,20,and 10 kDa,and their antigenicities were evaluated using indirect competitive enzyme-linked immunosorbent *** results showed that the degree of hydrolysis(DH)ofβ-conglycinin decreased as theβ-conglycinin concentration(S0)increased,but increased with enzyme dosage(E0)***,the enzymatic hydrolysis ofβ-conglycinin followed the first-order kinetics *** hydrolysis rate(V)was(527.89C_(E0)-2.5533C_(S0))exp(-0.022DH),the DH-hydrolysis time was 45.454ln[1+(11.614C_(E0)/C_(S0)-0.0562)t],and the correlated kinetic constants k2 and kd were 527.89 min^(−1)and 8.6126 min^(−1),*** hydrolysis behavior ofβ-conglycinin varied considerably among theα',α,andβ*** hydrolysis rates were observed for theα'andαsubunits compared to theβ*** relative molecular weights of the intercepted peptides from the hydrolysates were 14.8-40.1 kDa,and the antigenicity of the peptides with smaller molecular weight was reduced,but not removed ***,the alkaline protease from the strain appeared to effectively reduce the allergenicity ofβ-***,it is possible to produce less allergenic soybean

关键词： B.subtilis ACCC 01746 Alkaline protease β-Conglycinin Enzymatic kinetics Antigenicity

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鳖蛋黄血管紧张素转化酶抑制肽分离纯化及活性分析

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食品科学 2025年第1期46卷 40-48页

作者：刘华宇廖彭莹张天丰张新锐邓纭宁李耀华韦金锐陈俊广西中医药大学药学院广西南宁530200 广西中医药大学广西高校中药提取纯化与质量分析重点实验室中药学国家级实验教学示范中心广西南宁530200 广西中医药大学教学实验实训中心广西南宁530200 广西中医药大学广西中医药科学实验中心广西南宁530200

为从鳖蛋黄酶解物中筛选具有血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制活性的肽段,以ACE抑制活性为评价指标,采用超滤和凝胶过滤色谱技术进行分离纯化。采用液相色谱-串联质谱技术对活性组分进行肽段鉴定,借助生物信息... 详细信息

为从鳖蛋黄酶解物中筛选具有血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制活性的肽段,以ACE抑制活性为评价指标,采用超滤和凝胶过滤色谱技术进行分离纯化。采用液相色谱-串联质谱技术对活性组分进行肽段鉴定,借助生物信息学工具进行活性评估。优选预测活性较高的肽段进行合成和活性验证,并用分子对接工具分析活性肽与ACE的相互作用。结果表明,鳖蛋黄菠萝蛋白酶酶解物的水解度为(17.70±0.34)%,抑制ACE的半抑制浓度(half maximal inhibitory concentration,IC_(50))值为(0.210±0.019)mg/mL。对酶解产物进行分离纯化,从活性组分F3中鉴定出36条肽段,选择6条活性评分较高的肽段进行合成,其中肽段YNGIWPRD和ASDILPKK的IC_(50)值分别为(0.019 00±0.000 36)、(0.170 0±0.001 3)mg/mL。分子对接结果表明,二者均通过多条氢键与ACE紧密结合。综上,从鳖蛋黄中筛选出2条新的ACE抑制活性肽。

关键词：鳖蛋黄血管紧张素转化酶抑制肽分离纯化生物信息学分子对接

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Pore Formation Mechanism in W-C Hard Coatings Using Directed Energy Deposition on Tungsten Alloy

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Acta Metallurgica Sinica(English Letters) 2024年第1期37卷 89-101页

作者： xinrui zhang Weijie Fu Chen Wang Zhenglong Lei Haoran Sun Xudong Li State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin150001China

Porosity is a common phenomenon and can significantly hinder the quality of the ***,the pore formation mechanism and the characteristics of the single tracks of the W-C coating using directed energy deposition(DED)are systematically *** forming quality of the tracks,the distribution of the pores,and the elemental distribution near the pores are analyzed by the observations of the cross-sections of the *** temperature field of the melt pool is discussed comprehensively to reveal the pore formation *** results confirm that Ni and Co evaporated during the DED process due to the high temperature of the melt *** were continuously produced adjacent to the fusion line when the melt pool was about to solidify since the temperature at the solidification front was higher than the boiling point of *** vaporization area at the fusion line was proposed,where Ni could also evaporate at the time the melt pool started to *** relationship between the solidification rate,the size of the vaporization area and the DED parameters(laser power and scanning speed)was established to discuss the causes of severe pores above the fusion *** work contains a practical guide to reduce or eliminate the porosity in the coating preparation process on the surface of the tungsten alloy.

关键词： Directed energy deposition(DED)process Tungsten alloy W-C coating Pores Binder phase

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Molecular design of recyclable thermosetting polyimide and its composite with excellent mechanical and tribological properties

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Friction 2024年第3期12卷 452-461页

作者： Xiaoyue WANG Zenghui YANG Tingmei WANG Qihua WANG xinrui zhang Song LI Key Laboratory of Science and Technology on Wear and Protection of Materials Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

Recyclability of thermosetting polymers and their composites is a challenge for alleviating environmental pollution and resource *** this study,solvent-recyclable thermosetting polyimide(PI)and its composite were successfully *** tensile strength,elongation at break,and Young’s modulus of PI are 108.70±7.29 MPa,19.35%±3.89%,and 2336.42±128.00 MPa,*** addition of reduced graphene oxide(RGO)not only enhances the mechanical properties of PI but also endows it with excellent tribological *** PI illustrates a high recycling efficiency of 94.15%,but the recycled composite exhibits inferior mechanical *** recycling and utilization of PI and its composite are realized through imine bonds(-C=N),which provides new guidance for solving the problem of environmental pollution and resource waste and is potential application in the field of sustainable tribology.

关键词： thermosetting polyimide(PI) recyclability dynamic covalent bonds friction and wear polymer composites

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