Enzymatic kinetics ofβ-conglycinin using alkaline protease from Bacillus subtilis ACCC 01746 and analysis of antigenicity of hydrolyzed peptide

作     者：Haicheng Yin Xinrui Zhang Zhixiang Yang Jin Huang Haicheng Yin;Xinrui Zhang;Zhixiang Yang;Jin Huang

作者机构：College of Biological EngineeringHenan University of TechnologyZhengzhou 450001China 

出 版 物：《Grain & Oil Science and Technology》 (粮油科技（英文版）)

年 卷 期：2021年第4卷第3期

页      面：141-148页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：Authors wish to thank to Grain&Corn Engineering Technology Research Center State Administration of Grain(GA2017004)for funding support 

主　　题：B.subtilis ACCC 01746 Alkaline protease β-Conglycinin Enzymatic kinetics Antigenicity 

摘      要：β-Conglycinin,the main protein of soybean,is a key allergen that causes soybean allergies,and hydrolysis is usually applied to lower its *** evaluated the enzymolysis characters ofβ-conglycinin from the perspective of enzymolysis kinetics using alkaline protease from *** ACCC 01746.A dynamic model describing the hydrolysis ofβ-conglycinin was proposed using the initial substrate concentration,enzyme dosage(enzyme to substrate ratio)and hydrolysis time as variables to illustrate the kinetic behavior of enzymatic *** hydrolysis of soybeanβ-conglycinin was carried out at 60 g/L protein concentration,0.6%enzyme dosage,55℃ and pH 8.5 to observe the peptides with anti-enzymatic *** hydrolysates were gradually fractionated by ultrafiltration through cut-off membranes with molecular weights of 40,30,20,and 10 kDa,and their antigenicities were evaluated using indirect competitive enzyme-linked immunosorbent *** results showed that the degree of hydrolysis(DH)ofβ-conglycinin decreased as theβ-conglycinin concentration(S0)increased,but increased with enzyme dosage(E0)***,the enzymatic hydrolysis ofβ-conglycinin followed the first-order kinetics *** hydrolysis rate(V)was(527.89C_(E0)-2.5533C_(S0))exp(-0.022DH),the DH-hydrolysis time was 45.454ln[1+(11.614C_(E0)/C_(S0)-0.0562)t],and the correlated kinetic constants k2 and kd were 527.89 min^(−1)and 8.6126 min^(−1),*** hydrolysis behavior ofβ-conglycinin varied considerably among theα ,α,andβ*** hydrolysis rates were observed for theα andαsubunits compared to theβ*** relative molecular weights of the intercepted peptides from the hydrolysates were 14.8-40.1 kDa,and the antigenicity of the peptides with smaller molecular weight was reduced,but not removed ***,the alkaline protease from the strain appeared to effectively reduce the allergenicity ofβ-***,it is possible to produce less allergenic soybean