Hypoxia-mediated programmed cell death is involved in the formation of wooden breast in broilers

作     者：Xinrui Zhang Tong Xing Lin Zhang Liang Zhao Feng Gao 

作者机构：College of Animal Science and TechnologyKey Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu ProvinceJiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety ControlNanjing Agricultural UniversityNo.1 WeigangNanjingJiangsu 210095P.R.China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2024年第15卷第5期

页      面：1918-1931页

核心收录：

学科分类：1002[医学-临床医学] 0905[农学-畜牧学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(32072780 and 32272900) the Earmarked Fund for Jiangsu Agricultural Industry Technology System(JATS418) 

主　　题：Apoptosis Autophagy Broiler chicken Hypoxia Necroptosis Wooden breast 

摘      要：Background Wooden breast(WB)myopathy is a common myopathy found in commercial broiler chickens *** examination has revealed that WB myopathy is accompanied by damage to the pectoralis major(PM)***,the underlying mechanisms responsible for the formation of WB in broilers have not been fully *** study aimed to investigate the potential role of hypoxia-mediated programmed cell death(PCD)in the formation of WB *** Histological examination and biochemical analysis were performed on the PM muscle of the control(CON)and WB groups.A significantly increased thickness of the breast muscle in the top,middle,and bottom portions(P0.01)was found along with pathological structure damage of myofibers in the WB *** number of capillaries per fiber in PM muscle,and the levels of p O_(2) and s O_(2) in the blood,were significantly decreased(P0.01),while the levels of p CO_(2) and TCO_(2) in the blood were significantly increased(P0.05),suggesting hypoxic conditions in the PM muscle of the WB *** further evaluated the PCD-related pathways including autophagy,apoptosis,and necroptosis to understand the consequence response to enhanced hypoxic conditions in the PM muscle of birds with *** ratio of LC3 II to LC3 I,and the autophagy-related factors HIF-1α,BNIP3,Beclin1,AMPKα,and ULK1 at the m RNA and protein levels,were all significantly upregulated(P0.05),showing that autophagy occurred in the PM muscle of the WB *** apoptotic index,as well as the expressions of Bax,Cytc,caspase 9,and caspase 3,were significantly increased(P0.05),whereas Bcl-2 was significantly decreased(P0.05)in the WB-affected PM muscle,indicating the occurrence of apoptosis mediated by the mitochondrial ***,the expressions of necroptosis-related factors RIP1,RIP3,and MLKL,as well as NF-κB and the pro-inflammatory cytokines TNF-α,IL-1β,and IL-6,were all significantly enhanced(P0.05)in the WB-affected PM *** T